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Novel growth factor of Bombyx mori granulocyte

Research Project

Project/Area Number 18380039
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

KIKUO Iwabuchi  Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Professor (00203399)

Co-Investigator(Kenkyū-buntansha) SATO Ryoich  Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Associate Professor (30235428)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥11,080,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2007: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2006: ¥6,400,000 (Direct Cost: ¥6,400,000)
KeywordsBombyx mori / hemocyte / granulocyte / growth factor / insulin / IDGF / カイコ / 細胞・組織 / 生理活性
Research Abstract

Insect hemocytes are thought to be supplied by hemopoiesis in the hematopoietic organs or by mitotic division of circulating hemocytes. Our previous study demonstrated that Bombyx mori granulocytes are produced by prohemocyte differentiation and granulocyte division. Moreover, bombyxin acts cooperatively with hemolymph factor(s) to stimulate mitotic division of granulocytes. Bovine insulin is also active and substitutes for bombyxin. This research was carried out to identify the Bombyx mori granulocyte growth factor(s). In the concrete, (1) analysis of the factor(s) from larval hemolymph and (2) determination of BmIDGF based on the genetic data base of DmIDGF which acts cooperatively with insulin in Drosophila are carried out with in vitro biological assay.
A previous study showed that the factor(s) may be high molecular weight protein with thermal stability and hemolymph collected from L5D2 larvae is most active. The factor(s) has been purified by a combination of ultrafiltration, ammonium sulfate fractionation and the HPLC system of DEAE ion-exchange column and then gel filtration column. The granulocyte growth factor was composed of two molecules, approximately 20kDa protein which acts in collaboration with insulin and approximately 95 kDa protein acting without other molecules. The amino acid analysis revealed that both were novel growth factors.
The DNA sequence of Bombyx mori imaginal disc growth factor (BmIDGF) was determined by using cDNA library made from B. mori fat body mRNA and primers designed based on the DNA sequence database of DmIDGF. BmIDGF peptide made by using Baculovirus expression system with Sf9 cells did not show growth promoting activity, suggesting that the granulocyte growth factor(s) are the molecules described above.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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