Herbivore-plant interaction by proteome analysis

• Project/Area Number: 18380042
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: SUGIMURA Yukio Kyoto Institute of Technology, 工芸科学研究科, 教授 (20273542)
• Co-Investigator(Kenkyū-buntansha): OYAMA Hiroshi 日本薬科大学, 薬学部, 教授 (50221700) ; NAGAOKA Sumiharu 京都工芸繊維大学, 工芸科学研究科, 助教 (00303933)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥8,680,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥1,380,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2006: ¥2,700,000 (Direct Cost: ¥2,700,000)
• Keywords: 植物 / 昆虫 / タンパク / プロテオーム / 酵素 / クワウレアーゼ / ウレアーゼ結合タンパク質 / クワ葉タンパク質の取り込み / カイコ体液中の外来タンパク質 / プロテオーム解析

Research Abstract

クワ-カイコ実験系を用いて、タンパク質レベルでの相互関係を明らかにした。カイコ体内に取込まれるクワウレアーゼのaccessory proteins (ureD, ureF, ureG)遺伝子を単離・同定した。また、クワ幼苗でのウレアーゼ活性とそれら遺伝子の発現パターンを明らかにした。一方、カイコに取込まれる7種の新規タンパク質を見出し、その1つはsulfotransferase類似タンパク質であると同定した。

Research Products

• [Journal Article] Localization of mucilaginous polysaccarides in mulberry leaves (2008)
  • Author(s): Katayama,H., Takano,R., Sugimura,Y
  • Journal Title: Protoplasma 233 Pages: 157-163
  • Peer Reviewed
• [Journal Article] Ultrastructural and immunochemical features of cell wall sac formed in mulberry (Morus alba) idioblasts (2008)
  • Author(s): Katayama,H., Fujibayashi,Y., Nagaoka,S., Sugimura,Y
  • Journal Title: J. Plant Res. 121 Pages: 201-205
  • NAID: 10021087862
  • Peer Reviewed
• [Journal Article] Localization of mucilaginous polysaccharides in mulberry leaves (2008)
  • Author(s): Katayama, H.
  • Journal Title: Protoplasma 233 Pages: 157-163
  • Peer Reviewed
• [Journal Article] Ultrastructural and immunochemical features of the cell wall sac formed in mulberry (Morus alba) idioblasts. (2008)
  • Author(s): Katayama, H.
  • Journal Title: J. Plant Res. 121 Pages: 201-205
  • Peer Reviewed
• [Journal Article] Novel inhibitor for prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis and details of substrate-recognition mechanism (2008)
  • Author(s): Xu, Y.
  • Journal Title: J. Mol. Biol. 375 Pages: 708-719
  • Peer Reviewed
• [Journal Article] Cytological changes during cell wall sac formation in mulberry idioblasts (2007)
  • Author(s): Sugimura,Y., Nitta,I
  • Journal Title: Protoplasma 231 Pages: 123-125
  • Peer Reviewed
• [Journal Article] Cell wall sheath surrounding calcium oxalate crystals in mulberry idioblasts (2007)
  • Author(s): Katayama,H., Fujibayashi,Y., Nagaoka,S., Sugimura,Y
  • Journal Title: Protoplasma 231 Pages: 245-248
  • Peer Reviewed
• [Journal Article] Molecular characterization of a cDNA encoding extracellular dsRNase and its expression in the silkworm, Bombyx mori (2007)
  • Author(s): Arimatsu, Y.
  • Journal Title: Insect Biochem. Mol. Biol. 37 Pages: 176-183
  • Peer Reviewed
• [Journal Article] Purification and properties of double-stranded RNA-degrading nuclease, dsRNase, from the digestive juice of the silkworm, Bombyx mori (2007)
  • Author(s): Arimatsu, Y.
  • Journal Title: J. Insect Biotechnol. Sericol. 76 Pages: 57-62
  • Peer Reviewed
• [Journal Article] Cytological changes during cell wall sac formation in mulberry idioblasts (2007)
  • Author(s): Sugimura, Y.
  • Journal Title: Protoplasma 231 Pages: 123-125
  • Peer Reviewed
• [Journal Article] Cell wall sheath surrounding calacium oxalate crystals in mulberry Idioblasts (2007)
  • Author(s): Katayama, H.
  • Journal Title: Protoplasma. 231 Pages: 245-248
  • Peer Reviewed
• [Journal Article] Oligonucleotide transformation for the study of mutagenic specificities of DNA lesions in yeast (2007)
  • Author(s): Negishi, K.
  • Journal Title: Nucleic Acids Symp. Ser. (Oxf) 51 Pages: 211-212
• [Journal Article] 耐熱性セリン-カルボキシルプロテアーゼ、kumamolisinの構造と機能 (2007)
  • Author(s): 尾山 廣
  • Journal Title: 化学と生物. 45 Pages: 156-158
• [Journal Article] クワ葉におけるカルシウム集積の場-その構造と機能- (2007)
  • Author(s): 杉村順夫
  • Journal Title: 蚕糸・昆虫バイオテック 76 Pages: 137-140
  • NAID: 10019832998
• [Journal Article] Molecular characterization of a cDNA encoding extracellular dsRNase and its expression in the silkworm, Bombyx mori. (2007)
  • Author(s): Arimatsu, Y. et al.
  • Journal Title: Insect Biochem. Mol. Biol. 37 Pages: 176-183
• [Journal Article] Calcium carbonate deposition in a cell wall sac formed in mulberry idioblasts (2006)
  • Author(s): Nitta,I., Kida,A., Fujibayashi,Y.,Katayama, H., Sugimura,Y
  • Journal Title: Protoplasma 228 Pages: 201-208
  • Peer Reviewed
• [Journal Article] Purification and properties of two types of soluble trehalases from embryonic larvae of the silkworm, Bombyx mori. (2006)
  • Author(s): Huang, L. et al.
  • Journal Title: J. Insect Biotech. Sericology 75 Pages: 1-8
• [Journal Article] Calcium carbonate deposition in a cell wall sac formed in mulberry idioblasts. (2006)
  • Author(s): Nitta, I. et al.
  • Journal Title: Protoplasma 228 Pages: 201-208
• [Journal Article] Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site. (2006)
  • Author(s): Okubo, A. et al.
  • Journal Title: FEBS Journal 273 Pages: 2563-2576
• [Presentation] 絹糸腺由来の無細胞翻訳系におけるPlautia stall intestine virus (PSIV)-IRESの機能解析 (2009)
  • Author(s): 長岡純治
  • Organizer: 日本蚕糸学会第79回学術講演会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2009-03-22
• [Presentation] カイコ幼虫体内へ取り込まれるクワ葉タンパク質の同定 (2008)
  • Author(s): 大西祐作・間宮寛之・長岡純治・杉村順夫
  • Organizer: 日本蚕糸学会九州支部・関西支部合同大会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-11-14
• [Presentation] カイコ幼虫体内へ取り込まれるクワ葉タンパク質の同定 (2008)
  • Author(s): 大西祐作
  • Organizer: 日本蚕糸学会第64回九州支部・第74回関西支部合同大会
  • Place of Presentation: 九州大学
  • Year and Date: 2008-11-14
• [Presentation] クワ巨細胞におけるCdおよびZnの局在性 (2008)
  • Author(s): 馬場奈中央登
  • Organizer: 日本蚕糸学会第64回九州支部・第74回関西支部合同大会
  • Place of Presentation: 九州大学
  • Year and Date: 2008-11-14
• [Presentation] カイコガ無細胞翻訳系におけるタンパク質合成能力に関する改善方法の検討 (2008)
  • Author(s): 長岡純治
  • Organizer: 日本蚕糸学会第64回九州支部・第74回関西支部合同大会
  • Place of Presentation: 九州大学
  • Year and Date: 2008-11-13
• [Presentation] カイコ中腸組織からのウレアパク質の分離 (2008)
  • Author(s): 大西祐作・間宮寛之・長岡純治・杉村順夫
  • Organizer: 日本蚕糸学会第78回大会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-03-21
• [Presentation] Molecular characterization of a c-DNA encoding extracellular dsRNase and its expression in the silkworm, Bombyx mori. (2008)
  • Author(s): Arimatsu, Y.
  • Organizer: Asia-Pacific Congress of Sericulture and Insect Biotechnology (2008)
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-03-21
• [Presentation] Biomineralization function in mulberry leaves (2008)
  • Author(s): Sugimura, Y.
  • Organizer: Asia-Pacific Congress of Sericulture and Insect Biotechnology (2008)
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-03-21
• [Presentation] カイコ中腸組織からのウレアーゼ結合タンパク質の分離 (2008)
  • Author(s): 大西祐作, ら
  • Organizer: 日本蚕糸学会第78回大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-03-20
• [Presentation] クワ種子発芽時におけるウレアーゼ活性に関わる遺伝子の発現 (2007)
  • Author(s): 中山美緒・間宮寛之・長岡純治・杉村順夫
  • Organizer: 日本蚕糸学会九州支部・関西支部合同大会
  • Place of Presentation: 京都市
  • Year and Date: 2007-11-09
• [Presentation] クワ種子発芽時におけるウレアーゼ活性に関わる遺伝子の発現 (2007)
  • Author(s): 中山美緒, ら
  • Organizer: 日本蚕糸学会関西・九州支部合同大会
  • Place of Presentation: 京都工芸繊維大学
  • Year and Date: 2007-11-09
• [Presentation] カイコ体液から検出されるクワ葉タンパク質 (2007)
  • Author(s): 間宮寛之・長岡純治・杉村順夫・平山 力
  • Organizer: 日本蚕糸学会第77回大会
  • Place of Presentation: 筑波市
  • Year and Date: 2007-04-04
• [Presentation] クワ種子発芽期におけるウレアーゼ活性の発現 (2007)
  • Author(s): 中山美緒・間宮寛之・長岡純治・杉村順夫
  • Organizer: 日本蚕糸学会第77回大会
  • Place of Presentation: 筑波市
  • Year and Date: 2007-04-04
• [Presentation] カイコ体液から検出されるクワ葉タンパク質 (2007)
  • Author(s): 間宮寛之, ら
  • Organizer: 日本蚕糸学会第77回大会
  • Place of Presentation: 農林水産技術会議事務局筑波事務所(筑波)
  • Year and Date: 2007-04-04
• [Presentation] クワ種子発芽時におけるウレアーゼ活性の発現 (2007)
  • Author(s): 中山美緒, ら
  • Organizer: 日本蚕糸学会第77回大会
  • Place of Presentation: 農林水産技術会議事務局筑波事務所(筑波)
  • Year and Date: 2007-04-04
• [Presentation] 種子発芽時におけるクワウレアーゼ、UreG遺伝子の単離および発現 (2006)
  • Author(s): 長岡純治・中山美緒・高山尊之・間宮寛之・杉村順夫
  • Organizer: 日本蚕糸学会九州支部・関西支部合同大会
  • Place of Presentation: 福岡市
  • Year and Date: 2006-11-10
• [Book] クワタンパク質を利したカイコの生存戦略. 虫たちが語る生物学の未来 (2009)
  • Author(s): 間宮寛之・杉村順夫
  • Publisher: (財)衣笠会
• [Book] Plant-herbivore interaction, with special references to mulberry leaves and the silkworm, Bombyx mori. Insect Physiology: New Research (ed. R.P.Maes) (2008)
  • Author(s): Sugimura,Y., Nagaoka,S
  • Publisher: Nova Sci. Publishers, New York
• [Book] Insect Physiology : New research (2008)
  • Author(s): Sugimura, Y.
  • Publisher: Nova Science Publishers