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Gene expression network analysis for developing advanced utilization technology of cultured insect cells

Research Project

Project/Area Number 18380044
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionYamaguchi University

Principal Investigator

KOBAYASHI Jun  Yamaguchi University, 農学部, 教授 (70242930)

Project Period (FY) 2006 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥17,320,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2006: ¥9,000,000 (Direct Cost: ¥9,000,000)
Keywords昆虫細胞 / 遺伝子発現ネットワーク / カイコ / ゲノム / マイクロアレイ / 培養細胞 / Ras / piggyBac / transposase / アセチルコリンエステラーゼ / 脂質修飾 / 濃核病ウイルス / promoting protein / トランスポゼース / ピリダリル / アセチルコチンエステラーゼ / 代謝 / RAPD / クワコ
Research Abstract

As fundamental knowledge for developing advanced utilization technology of cultured insect cells, gene expression networks of the silkworm cells have been analyzed comrehensively using the oligo-DNA microarray and a number of genes changing their expression levels under different culture conditions have been successfully detected. In addition, piggyBac vector system required for establishing stable transformed cells, and transient expression vector-based in vitro assay systems effective for developing safer insecticides and elucidating infection mechanisms of insect viruses have been successfully produced.

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report   Self-evaluation Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (56 results)

All 2010 2009 2008 2007 Other

All Journal Article (13 results) (of which Peer Reviewed: 12 results) Presentation (35 results) Book (5 results) Remarks (3 results)

  • [Journal Article] Bombyx mori Ras proteins, BmRas1, BmRas2 and BmRas3 are neither farnesylated nor palmitoylated but are geranylgeranylated2009

    • Author(s)
      Moriya, K., Tsubota, T., Ishibashi, N., Yafune, A., Suzuki, T., Kobayashi, J., Shiotsuki, T., Utsumi, T.
    • Journal Title

      Insect Molecular Biology (published online)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] cDNA cloning of aquaporin gene expressed in the digestive tract of the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera; Rhinotermitidae)2009

    • Author(s)
      Kambara, K., Takematsu, Y., Azuma, T., Kobayashi, J.
    • Journal Title

      Applied Entomology and Zoology 44

      Pages: 3-770

    • NAID

      130000143010

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bombyx mori Ras proteins, BmRas1, BmRas2 and BmRas3 are neither farnesylated norpalmitoylated but are geranylgeranylated.2009

    • Author(s)
      Koko Moriya, et al.
    • Journal Title

      Insect Molecular Biology (published online)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] cDNA cloning of aquaporin gene expressed in the digestive tract of the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera ; Rhinotermitidae).2009

    • Author(s)
      Kohei Kambara, et al.
    • Journal Title

      Applied Entomology and Zoology 44

      Pages: 315-321

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a novel preparation method of recombinant proteoliposomes using baculovirus gene expression systems2008

    • Author(s)
      Fukushima, H., Mizutani, M., Imamura, K., Morino, K., Kobayashi, J., Okumura, K., Tsumoto, K., Yoshimura, T.
    • Journal Title

      Journal of Biochemistry 144

      Pages: 763-770

    • NAID

      10024914034

    • Related Report
      2009 Final Research Report 2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Pyridalyl inhibits cellular protein syntesis in insect, but not mammlian, cell lines2008

    • Author(s)
      Moriya, K., Hirakura, S., Kobayashi, J., Ozoe, Y., Saito, S., Utsumi, T.
    • Journal Title

      Archives of Insect Biochemistry and Physiology 69

      Pages: 22-31

    • Related Report
      2009 Final Research Report 2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Pyridalyl inhibits cellular protein syntesis in insect, but not mammlian cell lines2008

    • Author(s)
      Koko Moriya, et al.
    • Journal Title

      Archives of Insect Biochemistry and Physiology 69

      Pages: 22-31

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a novel preparation method of recombinant proteoliposomes using baculovirus gene expression systems.2008

    • Author(s)
      Hidetaka. Fukushiuma, et al.
    • Journal Title

      Journal of Biochemistry 144

      Pages: 763-770

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pyridalyl inhibits cellular protein syntesis in insect,but not mammlian cell lines2008

    • Author(s)
      Koko Moriya, et. al.
    • Journal Title

      Archives of Insect Biochemistry and Physiology 68(in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nucleotide sequence analysis of PstI D fragment of Antheraea pernyi nucleopolyhedrvirus clone A and identification of Escherichia coli insertion sequence2007

    • Author(s)
      Huang, Y., Mitsutake, H., Itakura, M., Wang, X., Kobayashi, J.
    • Journal Title

      International Journal of Wild Silkmoth & Silk 12

      Pages: 1-8

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 野蚕を宿主とするバキュロウイルスベクター系の魅力と実用化への挑戦2007

    • Author(s)
      小林淳
    • Journal Title

      蚕糸・昆虫バイオテック 76

      Pages: 189-196

    • NAID

      10021221382

    • Related Report
      2009 Final Research Report
  • [Journal Article] Sequence Analysis of PstI D Fragment of Antheraea pernyi Nucleopolyhedrovirus Clone D and Identification of Escherichia coli Insertion Sequence2007

    • Author(s)
      Yuan Jiao Huang, et. al.
    • Journal Title

      International Journal of Wild Silkworm&Silk 12

      Pages: 1-8

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 野蚕を宿主とするバキュロウイルスベグター系の魅力と実用化への挑戦2007

    • Author(s)
      小林 淳
    • Journal Title

      蚕糸・昆虫バイオテック 76

      Pages: 189-196

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 形質転換カイコ培養細胞のクローニングおよび導入遺伝子の安定性解析2010

    • Author(s)
      光武宏, 小林淳
    • Organizer
      日本蚕糸学会第80回記念大会
    • Place of Presentation
      信州大学
    • Year and Date
      2010-04-04
    • Related Report
      2009 Final Research Report
  • [Presentation] 形質転換カイコ培養細胞のクローニングおよび導入遺伝子の安定性解析2010

    • Author(s)
      光武宏, ほか
    • Organizer
      日本蚕系学会第80回記念大会
    • Place of Presentation
      信州大学
    • Year and Date
      2010-04-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] カイコras遺伝子の解析とRasタンパク質の脂質修飾解析2010

    • Author(s)
      塩月孝博, 坪田拓也, 守屋康子, 石橋直人, 八舟宏典, 小林淳, 鈴木崇, 小倉岳彦, 内海俊彦
    • Organizer
      日本蚕糸学会第80回記念大会
    • Place of Presentation
      信州大学
    • Year and Date
      2010-04-03
    • Related Report
      2009 Final Research Report
  • [Presentation] 昆虫培養細胞におけるDNV-2抵抗性および感受性遺伝子の発現2010

    • Author(s)
      津田恵寛, 門野敬子, 城所久良子, 今西重雄, 伊藤克彦, 小林淳
    • Place of Presentation
      信州大学
    • Year and Date
      2010-04-03
    • Related Report
      2009 Final Research Report
  • [Presentation] 昆虫培養細胞におけるDNV-2抵抗性および感受性遺伝子の発現2010

    • Author(s)
      津田恵寛, ほか
    • Organizer
      日本蚕系学会第80回記念大会
    • Place of Presentation
      信州大学
    • Year and Date
      2010-04-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] カイコras遺伝子の解析とRasタンパク質の脂質修飾解析2010

    • Author(s)
      塩月孝博, ほか
    • Organizer
      日本蚕系学会第80回記念大会
    • Place of Presentation
      信州大学
    • Year and Date
      2010-04-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] サクサン核多角体病ウイルスの全ゲノム解読と他の分離株との比較2010

    • Author(s)
      津田恵寛, Huang, Y., 光武宏, 板倉真, 王学英, 小林淳
    • Organizer
      第54回日本応用動物昆虫学会大会
    • Place of Presentation
      千葉大学
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 転移酵素mRNAを用いたpiggyBacで形質転換した昆虫細胞における転移酵素活性残存期間の短縮2009

    • Author(s)
      光武宏, 小林淳
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Genome sequence of Antheraea pernyi nucleopolyhedrovirus used for baculovirus expression vector system2009

    • Author(s)
      Tsuda, Y., Mitsutake, H., Itakura, M., Huang, Y., Wang, X., Kobayashi, J.
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 転移酵素mRNAを用いたpiggyBacで形質転換した昆虫培養細胞における転移酵素活性残存期間の解析2009

    • Author(s)
      光武宏, ほか
    • Organizer
      日本蚕系学会第75回関西支部・第65回九州支部合同大会昆虫機能利用学術講演会
    • Place of Presentation
      山口大学
    • Year and Date
      2009-11-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] バキュロウイルス遺伝子発現ベクター系を利用したBmDNV-2抵抗性および感受性遺伝子産物の生産2009

    • Author(s)
      津田恵寛, ほか
    • Organizer
      日本蚕系学会第75回関西支部・第65回九州支部合同大会昆虫機能利用学術講演会
    • Place of Presentation
      山口大学
    • Year and Date
      2009-11-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] Development of a novel preparation method of recombinant proteoliposomes using baculovirus gene expression systems2009

    • Author(s)
      Fukushima, H., Mizutani, M., Imamura, K., Morino, K., Kobayashi, J., Okumura, K., Tsumoto, K., Yoshimura, T.
    • Organizer
      第82回生化学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-10-23
    • Related Report
      2009 Final Research Report
  • [Presentation] Development of a novel preparation method of recombinant proteoliposomes using baculovirus geneexpression systems2009

    • Author(s)
      Hidetaka Fukushima, et al.
    • Organizer
      第82回生化学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] 転移酵素mRNAを用いたpiggyBacによる昆虫培養細胞の形質転換技術の改良2009

    • Author(s)
      光武宏, 小林淳
    • Organizer
      日本蚕糸学会第79回大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2009-03-22
    • Related Report
      2009 Final Research Report
  • [Presentation] 転移酵素mRNAを用いたpiggyBacによる昆虫培養細胞の形質転換技術の改良2009

    • Author(s)
      光武宏, ほか
    • Organizer
      日本蚕糸学会第79回大会
    • Place of Presentation
      東京農工大学農学部府中
    • Year and Date
      2009-03-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] バキュロウイルス遺伝子発現ベクター系を利用したDNV-2抵抗性遺伝子産物の生産2009

    • Author(s)
      津田恵寛, 門野敬子, 城所久良子, 伊藤克彦, 小林淳
    • Organizer
      日本蚕糸学会第79回大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2009-03-21
    • Related Report
      2009 Final Research Report
  • [Presentation] BmDNV-2感受性遺伝子( +n s d- 2)の強制発現による感受性誘導の試み2009

    • Author(s)
      門野敬子, 城所久良子, 津田恵寛, 今井啓太, 光武宏, 小林淳, 伊藤克彦, 瀬筒秀樹, 内野恵郎, 小林功, 田村俊樹, 三田和英
    • Organizer
      日本蚕糸学会第79回大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2009-03-21
    • Related Report
      2009 Final Research Report
  • [Presentation] BmDNV-2感受性遺伝子(+^<nsd-2>)の強制発現による感受性誘導の試み2009

    • Author(s)
      門野敬子, ほか
    • Organizer
      日本蚕糸学会第79回大会
    • Place of Presentation
      東京農工大学農学部府中
    • Year and Date
      2009-03-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] バキュロウイルス遺伝子発現ベクター系を利用したDNV-2抵抗性遺伝子産物の生産2009

    • Author(s)
      津田恵寛, ほか
    • Organizer
      日本蚕糸学会第79回大会
    • Place of Presentation
      東京農工大学農学部府中
    • Year and Date
      2009-03-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] 転移酵素mRNAを用いたpiggyBacによる昆虫培養細胞の形質転換2008

    • Author(s)
      光武宏, 小林淳
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-10
    • Related Report
      2009 Final Research Report 2008 Self-evaluation Report
  • [Presentation] 転移酵素mRNAを用いたpiggyBacによる昆虫培養細胞の形質転換2008

    • Author(s)
      光武宏, ほか
    • Organizer
      第31回に本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド神戸
    • Year and Date
      2008-12-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] カイコ体液promoting proteinによるKel細胞におけるBmNPV感染促進2008

    • Author(s)
      今井啓太, ほか
    • Organizer
      日本蚕糸学会第64回九州支部・第74回関西支部合同大会
    • Place of Presentation
      九州大学西新プラザ福岡
    • Year and Date
      2008-11-14
    • Related Report
      2008 Annual Research Report
  • [Presentation] piggyBacを利用した形質転換における転移酵素mRNAの5'UTRの重要性2008

    • Author(s)
      光武宏, ほか
    • Organizer
      日本蚕糸学会第64回九州支部・第74回関西支部合同大会
    • Place of Presentation
      九州大学西新プラザ福岡
    • Year and Date
      2008-11-14
    • Related Report
      2008 Annual Research Report
  • [Presentation] Attractive properties of baculovirus expression vector systems using wild silkmoths2008

    • Author(s)
      Kobayashi, J.
    • Organizer
      The 5th International Conference on Wild Silkmoths
    • Place of Presentation
      Phoenix Hotel (瀋陽, 中国)
    • Year and Date
      2008-09-10
    • Related Report
      2009 Final Research Report
  • [Presentation] Attractive properties of baculovirus expression vector systems using wild silkmoths2008

    • Author(s)
      Jun Kobayashi
    • Organizer
      The 5th International Conference on Wild Silkmoths
    • Place of Presentation
      Phoenix Hotel, Shenyang, CHINA
    • Year and Date
      2008-09-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] Effects of promoting protein on baculovirus susceptibility in Bombyx mori cell line NIAS-Ke-12008

    • Author(s)
      Imai, K., Kanaya, T., Imanishi, S., Kobayashi, J
    • Organizer
      Asia-Pacific Congress of Sericulture and Insect Biotechnology
    • Place of Presentation
      名古屋大学
    • Year and Date
      2008-03-22
    • Related Report
      2009 Final Research Report 2008 Self-evaluation Report
  • [Presentation] Performances of RAPD and DNA barcoding in idenfication and phylogenetic analysis of insect cell lines2008

    • Author(s)
      Seto, H., Fukuhara, A., Imanishi, S., Kobayashi, J.
    • Organizer
      Asia-Pacific Congress of Sericulture and Insect Biotechnology
    • Place of Presentation
      名古屋大学
    • Year and Date
      2008-03-22
    • Related Report
      2009 Final Research Report
  • [Presentation] Improvement of piggyBac-mediated stable transformation of cultured insect cells2008

    • Author(s)
      Mitsutake, H., Kobayashi, J.
    • Organizer
      Asia-Pacific Congress of Sericulture and Insect Biotechnology
    • Place of Presentation
      名古屋大学
    • Year and Date
      2008-03-22
    • Related Report
      2009 Final Research Report
  • [Presentation] バキュロウイルス感染細胞で特異的に発現するアシル化タンパク質の解析2007

    • Author(s)
      城所 智広, ほか
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] カイコバキュロウイルスベクター系を用いた殺虫剤抵抗性アセチルコリンエステラーゼの生産2007

    • Author(s)
      板倉真, 冨田隆史, 古崎利紀, 日堂修, 河野義明, 小林淳
    • Organizer
      日本蚕糸学会第77回大会
    • Place of Presentation
      農林水産技術会議事務局筑波事務所
    • Year and Date
      2007-04-04
    • Related Report
      2009 Final Research Report
  • [Presentation] 新たに作出した数種昆虫培養細胞のRAPD分析2007

    • Author(s)
      福原明子, 小林淳, 今西重雄
    • Organizer
      日本蚕糸学会第77回大会
    • Place of Presentation
      農林水産技術会議事務局筑波事務所
    • Year and Date
      2007-04-04
    • Related Report
      2009 Final Research Report
  • [Presentation] piggyBacベクターの改変と形質転換カイコ培養細胞における染色体挿入特性2007

    • Author(s)
      光武宏, 小林淳
    • Organizer
      日本蚕糸学会第77回大会
    • Place of Presentation
      農林水産技術会議
    • Year and Date
      2007-04-04
    • Related Report
      2009 Final Research Report
  • [Presentation] カイコバキュロウイルスベクター系を用いた殺虫剤抵抗性アセチルコリンエステラーゼの生産2007

    • Author(s)
      板倉 真, ほか
    • Organizer
      平成19年度蚕糸・昆虫機能利用学術講演会・日本蚕糸学会第77回大会
    • Place of Presentation
      農林水産技術会議事務局筑波事務所(茨城県つくば市)
    • Year and Date
      2007-04-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] 新たに作出した数種昆虫培養細胞のRAPD分析2007

    • Author(s)
      福原 明子, ほか
    • Organizer
      平成19年度蚕糸・昆虫機能利用学術講演会・日本蚕糸学会第77回大会
    • Place of Presentation
      農林水産技術会議事務局筑波事務所(茨城県つくば市)
    • Year and Date
      2007-04-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] piggyBacベクターの改変と形質転換カイコ培養細胞における染色体挿入特性2007

    • Author(s)
      光武 宏, ほか
    • Organizer
      平成19年度蚕糸・昆虫機能利用学術講演会・日本蚕糸学会第77回大会
    • Place of Presentation
      農林水産技術会議事務局筑波事務所(茨城県つくば市)
    • Year and Date
      2007-04-04
    • Related Report
      2007 Annual Research Report
  • [Book] の事典(渡邉信ほか編)(III. 5. 7バキュロウイルスベクターによる有用タンパク質の生産)2008

    • Author(s)
      小林淳
    • Publisher
      朝倉書店(東京)
    • Related Report
      2009 Final Research Report
  • [Book] 微生物の事典2008

    • Author(s)
      渡邊信, 他
    • Total Pages
      732
    • Publisher
      朝倉書店
    • Related Report
      2008 Annual Research Report
  • [Book] バイオプロセスハンドブック(有限会社ブッカーズ編)(第1編第7章昆虫工学の基礎)2007

    • Author(s)
      小林淳
    • Publisher
      NTS(東京)
    • Related Report
      2009 Final Research Report
  • [Book] 第1編第7章昆虫工学の基礎(有限会社ブッカーズ編)2007

    • Author(s)
      小林淳
    • Publisher
      バイオプロセスハンドブックNTS(東京)
    • Related Report
      2008 Self-evaluation Report
  • [Book] バイオプロセスハンドブック2007

    • Author(s)
      小林淳他126名
    • Total Pages
      724
    • Publisher
      株式会社 NTS
    • Related Report
      2006 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.yamaguchi-u.ac.jp/~member/kobayashi/p.html

    • Related Report
      2009 Final Research Report
  • [Remarks] 準備中

    • URL

      http://www.agr.yamaguchi-u.ac.jp/~member/kobayashi/p.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.yamaguchi-u.ac.jp/~member/kobayashi/p.html

    • Related Report
      2008 Self-evaluation Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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