Gene expression network analysis for developing advanced utilization technology of cultured insect cells
| Project/Area Number |
18380044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KOBAYASHI Jun Yamaguchi University, 農学部, 教授 (70242930)
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| Project Period (FY) |
2006 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥17,320,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2006: ¥9,000,000 (Direct Cost: ¥9,000,000)
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| Keywords | 昆虫細胞 / 遺伝子発現ネットワーク / カイコ / ゲノム / マイクロアレイ / 培養細胞 / Ras / piggyBac / transposase / アセチルコリンエステラーゼ / 脂質修飾 / 濃核病ウイルス / promoting protein / トランスポゼース / ピリダリル / アセチルコチンエステラーゼ / 代謝 / RAPD / クワコ |
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| Research Abstract |
As fundamental knowledge for developing advanced utilization technology of cultured insect cells, gene expression networks of the silkworm cells have been analyzed comrehensively using the oligo-DNA microarray and a number of genes changing their expression levels under different culture conditions have been successfully detected. In addition, piggyBac vector system required for establishing stable transformed cells, and transient expression vector-based in vitro assay systems effective for developing safer insecticides and elucidating infection mechanisms of insect viruses have been successfully produced.
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Report
(6 results)
Research Products
(56 results)
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[Journal Article] Bombyx mori Ras proteins, BmRas1, BmRas2 and BmRas3 are neither farnesylated nor palmitoylated but are geranylgeranylated2009
Author(s)
Moriya, K., Tsubota, T., Ishibashi, N., Yafune, A., Suzuki, T., Kobayashi, J., Shiotsuki, T., Utsumi, T.
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Journal Title
Insect Molecular Biology (published online)
Related Report
Peer Reviewed
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[Journal Article] Development of a novel preparation method of recombinant proteoliposomes using baculovirus gene expression systems2008
Author(s)
Fukushima, H., Mizutani, M., Imamura, K., Morino, K., Kobayashi, J., Okumura, K., Tsumoto, K., Yoshimura, T.
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Journal Title
Journal of Biochemistry 144
Pages: 763-770
NAID
Related Report
Peer Reviewed
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[Journal Article] Pyridalyl inhibits cellular protein syntesis in insect, but not mammlian, cell lines2008
Author(s)
Moriya, K., Hirakura, S., Kobayashi, J., Ozoe, Y., Saito, S., Utsumi, T.
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Journal Title
Archives of Insect Biochemistry and Physiology 69
Pages: 22-31
Related Report
Peer Reviewed
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[Presentation] Genome sequence of Antheraea pernyi nucleopolyhedrovirus used for baculovirus expression vector system2009
Author(s)
Tsuda, Y., Mitsutake, H., Itakura, M., Huang, Y., Wang, X., Kobayashi, J.
Organizer
第32回日本分子生物学会年会
Place of Presentation
パシフィコ横浜
Year and Date
2009-12-11
Related Report
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[Presentation] Development of a novel preparation method of recombinant proteoliposomes using baculovirus gene expression systems2009
Author(s)
Fukushima, H., Mizutani, M., Imamura, K., Morino, K., Kobayashi, J., Okumura, K., Tsumoto, K., Yoshimura, T.
Organizer
第82回生化学会大会
Place of Presentation
神戸国際会議場
Year and Date
2009-10-23
Related Report
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[Presentation] BmDNV-2感受性遺伝子( +n s d- 2)の強制発現による感受性誘導の試み2009
Author(s)
門野敬子, 城所久良子, 津田恵寛, 今井啓太, 光武宏, 小林淳, 伊藤克彦, 瀬筒秀樹, 内野恵郎, 小林功, 田村俊樹, 三田和英
Organizer
日本蚕糸学会第79回大会
Place of Presentation
東京農工大学
Year and Date
2009-03-21
Related Report
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