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Comprehensive development of bacterial ability for the degradation ofchlorinated environmental pollutants using unexplored genetic sources

Research Project

Project/Area Number 18380054
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionTohoku University

Principal Investigator

NAGATA Yuji  Tohoku University, 大学院・生命科学研究科, 准教授 (30237531)

Co-Investigator(Kenkyū-buntansha) TSUDA Masataka  東北大学, 大学院・生命科学研究科, 教授 (90172022)
Research Collaborator OHTSUBO Yoshiyuki  東北大学, 大学院・生命科学研究科, 助教
JIRI Damborsky  マサリク大学(チェコ共和国), 教授
SENDA Toshiya  産業技術総合研究所, 主任研究員
TANOKURA Masaru  東京大学, 大学院・農学生命科学研究科, 教授
TAKEYAM Haaruko  早稲田大学, 理工学術院, 教授
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥16,730,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2007: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2006: ¥10,100,000 (Direct Cost: ¥10,100,000)
Keywords環境微生物学 / 酵素 / 細菌 / バイオテクノロジー / 微生物 / 環境汚染物質
Research Abstract

環境汚染が深刻な代表的な有機塩素系殺虫剤に焦点をあて、(i) 難培養性微生物の遺伝情報も含む未開拓な遺伝子資源から新規の分解酵素遺伝子の取得、(ii) 有機塩素系環境汚染物質分解の鍵酵素である脱ハロゲン酵素の機能改良、(iii) 直接の分解酵素以外で分解活性の発現に必須な細胞因子の同定、(iv) 有効な有機塩素系化合物の生分解システムの構築、を実施し、環境細菌が有する高度難分解性有機塩素系化合物の分解能力に関する重要な基礎的知見を得ると共に、実際の環境浄化へ結びつけるための基盤を整備した。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (111 results)

All 2009 2008 2007 2006

All Journal Article (45 results) (of which Peer Reviewed: 38 results) Presentation (62 results) Book (4 results)

  • [Journal Article] 環境細菌ゲノムの構造と可塑性-難分解性化合物分解の総合職と専門職の場合-2009

    • Author(s)
      大坪嘉行, 永田裕二, 津田雅孝
    • Journal Title

      化学と生物 47(1)

      Pages: 35-42

    • NAID

      10026434544

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis ofa novel haloalkane dehalogenase DbeA fromBradyrhizobium elkani USDA94.2009

    • Author(s)
      Prudnikova, T. T. Mozga, P. Rezacova, R.Chaloupkova, Y. Sato, Y. Nagata, J. Brynda,M. Kuty, J. Damborsky, and I. K. Smatanova.
    • Journal Title

      Acta Crystallographica Section F 65

      Pages: 353-356

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA942009

    • Author(s)
      T. Prudnikova, et.al.
    • Journal Title

      Acta Crystallographica Section F 65

      Pages: 353-356

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 環境細菌ゲノムの構造と可塑性-難分解性化合物分解の総合職と専門職の場合-2009

    • Author(s)
      大坪嘉行ら
    • Journal Title

      化学と生物 47

      Pages: 35-42

    • NAID

      10026434544

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 多重染色体性のBurkholderia multivoransのゲノム構造と土壌でのゲノム情報発現2008

    • Author(s)
      津田雅孝, 西山依里, 宮腰昌利, 湯原悟志, 永田裕二, 大坪嘉行
    • Journal Title

      土と微生物 62 (2)

      Pages: 93-97

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] GenomeMatcher : A graphicaluser interface for DNA sequence comparison2008

    • Author(s)
      Ohtsubo, Y., W. Ikeda-Ohtsubo, Y. Nagata, and M. Tsuda
    • Journal Title

      BMC Bioinformatics 9

      Pages: 376-376

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the traD operonof naphthalene-catabolic plasmid NAH7 ahost-range modifier in conjugative transfer2008

    • Author(s)
      Miyazaki, R., Y. Ohtsubo, Y. Nagata, and M. Tsuda
    • Journal Title

      J. Bacteriol 190

      Pages: 6281-6289

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Construction of signature-taggedmutant library in Mesorhizobium loti as apowerful tool for functional genomics2008

    • Author(s)
      Shimoda, Y., H. Mitsui, H. Kamimatsuse,K. Minamisawa, E. Nishiyama, Y. Ohtsubo, Y.Nagata, M. Tsuda, S. Shinpo, A. Watanabe, M.Kohara, M. Yamada, Y. Nakamura, S. Tabata and S. Sato
    • Journal Title

      DNAResearch 15

      Pages: 297-308

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Insertion sequence-based cassette PCR cultivation-independent isolation of γ-hexachlorocyclohexane-degrading genes fromsoil DNA2008

    • Author(s)
      Fuchu, G., Y. Ohtsubo, M. Ito, R.Miyazaki, A. Ono, Y. Nagata, and M. Tsuda
    • Journal Title

      Appl. Microbiol.Biotechnol 79

      Pages: 627-632

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pleiotropic roles of iron-responsivetranscriptional regulator Fur in Burkholderiamultivorans2008

    • Author(s)
      Yuhara, S., H. Komatsu, H. Goto, Y.Otsubo, Y. Nagata, and M. Tsuda
    • Journal Title

      Microbiology 154

      Pages: 1763-1774

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pleiotropic roles of iron-responsive transcriptional regulator Fur in Burkholderia multivorans2008

    • Author(s)
      S. Yuhara, et.al.
    • Journal Title

      Microbiology 154

      Pages: 1763-1774

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insertion sequence-based cassette PCR : cultivation-independent isolation of γ-hexachlorocyclohexane-degrading genes from soil DNA2008

    • Author(s)
      G. Fuchu, et.al.
    • Journal Title

      Appl. Microbiol. Biotechnol. 79

      Pages: 627-632

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of signature-tagged mutant library in Mesorhizobium loti as a powerful tool for functional genomics2008

    • Author(s)
      Y. Shimoda, et.al.
    • Journal Title

      DNA Research 15

      Pages: 297-308

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the traD operon of naphthalene-catabolic plasmid NAH7 : a host-range modifier in conjugative transfer2008

    • Author(s)
      R. Miyazaki, et.al.
    • Journal Title

      J. Bacteriol. 190

      Pages: 6281-6289

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome Matcher : A graphical user interface for DNA sequence comparison2008

    • Author(s)
      Y. Ohtsubo, et.al.
    • Journal Title

      BMC Bioinformatics 9

      Pages: 376-376

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 多重染色体性のBurkholderia multivoransのゲノム構造と土壌でのゲノム情報発現2008

    • Author(s)
      津田雅孝ら
    • Journal Title

      土と微生物 62

      Pages: 93-97

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 土壌環境での細菌の生き様を探る(2008年度日本生物工学会大会報告)2008

    • Author(s)
      永田裕二
    • Journal Title

      日本生物工学会誌 86

      Pages: 549-549

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Complete sequencedetermination combined with analysis oftransposition/site-specific recombination eventsto explain genetic organization of IncP-7 TOLplasmid pWW53 and related mobile geneticelements2007

    • Author(s)
      Yano, H., C.E. Garruto, M. Sota, Y.Ohtsubo, Y. Nagata, G.J. Zylstra, P.A.Williams, and M. Tsuda
    • Journal Title

      J. Mol. Biol 369

      Pages: 11-26

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization ofgenes encoding a putative ABC-type transporteressential for the utilization of γ-hexachlorocyclohexane in Sphingobiumjaponicum UT262007

    • Author(s)
      Endo, R., Y. Ohtsubo, M. Tsuda, and Y.Nagata
    • Journal Title

      J. Bacteriol 189

      Pages: 3712-3720

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of β-hexachlorocyclohexane byhaloalkane dehalogenase LinB from γ-hexachlorocyclohexane-utilizing bacteriumSphingobium sp2007

    • Author(s)
      Ito, M., Z. Prokop, M. Klvana, Y. Otsubo, M. Tsuda, J. Damborsky, and Y. Nagata
    • Journal Title

      MI1205. Arch Microbiol 188

      Pages: 313-325

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aerobic degradation of lindane(γ-hexachlorocyclohexane)in bacteria and itsbiochemical and molecular basis. Appl2007

    • Author(s)
      Nagata, Y., R. Endo, M. Ito, Y. Ohtsubo and M. Tsuda
    • Journal Title

      Microbiol. and Biotechnol 76

      Pages: 741-752

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The identificationof catalytic pentad in the haloalkanedehalogenase DhmA from Mycobacterium aviumN85 : reaction mechanism and molecularevolution2007

    • Author(s)
      Pavlova, M., Klavana, A. Jesenska, Z.Prokop, H. Konecna, T. Sato, M. Tsuda, Y.Nagata, and J. Damborsky
    • Journal Title

      J. Struct. Biol 157

      Pages: 384-392

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation andcharacterization of naphthalene-catabolic genesand plasmids from oil-contaminated soil by usingtwo cultivation-independent approaches2007

    • Author(s)
      Ono, A., R. Miyazaki, M. Sota, Y. Ohtsubo,Y. Nagata, and M. Tsuda
    • Journal Title

      Appl.Microbiol. Biotechnol 74

      Pages: 501-510

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Senda.Crystallization and preliminary crystallographicanalysis of a haloalkane dehalogenase DbjA fromBradyrhizobium japonicum USDA1102007

    • Author(s)
      Sato, Y., R. Natsume, M. Tsuda, J. Damborsky, Y. Nagata and T
    • Journal Title

      ActaCryst Section F 63

      Pages: 294-296

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Weak activity ofhaloalkane dehalogenase LinB with1, 2, 3-trichloropropane revealed by X-raycrystallography and microcalorimetry2007

    • Author(s)
      Monincova, M., Z. Prokop, J. Vevodova, Y.Nagata and J. Damborsky
    • Journal Title

      Appl.Envirom. Microbiol 73

      Pages: 2005-2008

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 機能発現に基づく環境汚染物質分解酵素遺伝子の生態系からの直接的取得と解析2007

    • Author(s)
      津田雅孝、小野玲、宮崎亮、府中玄樹、永田裕二
    • Journal Title

      J. Environ. Biotechnol 7(2)

      Pages: 75-78

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 自然環境で実際に機能する微生物遺伝子の遺伝学的手法による検索と解析2007

    • Author(s)
      津田雅孝、西山依里、永田裕二、大坪嘉行
    • Journal Title

      化学と生物 45

      Pages: 557-563

    • NAID

      10019578182

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-ray crystallography and microcalorimetry2007

    • Author(s)
      Monincova, M., et. al.
    • Journal Title

      Appl. Envirom. Microbiol. 73

      Pages: 2005-2008

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary crystallographic analysis of a haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA1102007

    • Author(s)
      Sato, Y., et. al.
    • Journal Title

      Acta Cryst Section F 63

      Pages: 294-296

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete sequence determination combinedwith analysis of transposition/site-specific recombination events toexplain genetic organization of IncP-7 TOL plasmid pWW53 and related mobile genetic elements2007

    • Author(s)
      Yano, H., et. al.
    • Journal Title

      J. Mol. Biol. 369

      Pages: 11-26

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of genes encoding a putative ABC-type transporter essential for the utilization of γ-hexachlorocyclohexane in Sphingobium Japonicum UT262007

    • Author(s)
      Endo, R., et. al.
    • Journal Title

      J. Bacteriol. 189

      Pages: 3712-3720

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of β-hexachlorocyclohexane by haloalkane dehalogenase LinB from γ-hexachlorocyclohexane-utilizing bacterium Sphingobium sp. MI12052007

    • Author(s)
      Ito, M., et. al.
    • Journal Title

      Arch. Microbiol. 188

      Pages: 313-325

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aerobic degradation of lindane (γ-hexachlorocyclohexane)in bacteria and its biochemical and molecular basis2007

    • Author(s)
      Nagata, Y., et. al.
    • Journal Title

      Appl. Microbiol. and Biotechnol. 76

      Pages: 741-752

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 自然環境で実際に機能する微生物遺伝子の遺伝学的手法による検索と解析2007

    • Author(s)
      津田雅孝, ら
    • Journal Title

      化学と生物 45

      Pages: 557-563

    • NAID

      10019578182

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 機能発現に基づく環境汚染物質分解酵素遺伝子の生態系からの直接的取得と解析2007

    • Author(s)
      津田雅孝, ら
    • Journal Title

      J. Environ. Biotechnol. 7

      Pages: 75-78

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of naphthalene-catabolic genes and plasmids from oil-contaminated soil by using two cultivation-independent approaches2007

    • Author(s)
      Ono, A., et al.
    • Journal Title

      Appl. Microbiol. Biotechnol. 74

      Pages: 501-510

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The identification of catalytic pentad in the haloalkane dehalogenase DhmA from Mycobacterium avium N85 : reaction mechanism and molecular evolution2007

    • Author(s)
      Pavlova, M., et al.
    • Journal Title

      J. Struct. Biol. 157

      Pages: 384-392

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Completenucleotide sequence of an exogenously isolatedplasmid pLB1 involved in the degradation ofγ-hexachlorocyclohexane2006

    • Author(s)
      Miyazaki, R., Y. Sato, M. Ito, Y. Ohtsubo,Y. Nagata and M. Tsuda
    • Journal Title

      Appl. Envirom.Microbiol 72

      Pages: 6923-6933

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a responseregulator gene for catabolite control from aPCB-degrading β-proteobacteria, Acidovorax sp. KKS1022006

    • Author(s)
      Ohtsubo, Y., H. Goto, Y. Nagata, T. Kudo,and M. Tsuda
    • Journal Title

      Mol. Microbiol 60

      Pages: 1563-1575

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] ハロアルカンデハロゲナーゼの構造と機能2006

    • Author(s)
      永田裕二、津田雅孝
    • Journal Title

      J. Environ. Biotechnol 6

      Pages: 87-92

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 境汚染物質を分解する酵素遺伝子の土壌からの直接的取得と解析2006

    • Author(s)
      小野玲、宮崎亮、永田裕二、津田雅孝
    • Journal Title

      バイオインダストリー11月号 23(11)

      Pages: 44-49

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a response regulator gene for catabolite control from a PCB-degrading β-proteobacteria, Acidovorax sp. KKS1022006

    • Author(s)
      Ohtsubo, Y., et al.
    • Journal Title

      Mol. Microbiol. 60

      Pages: 1563-1575

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Complete nucleotide sequence of an exogenously isolated plasmid pLB1 involved in the degradation of γ-hexachlorocyclohexane2006

    • Author(s)
      Miyazaki, R., et al.
    • Journal Title

      Appl. Envirom. Microbiol. 72

      Pages: 6923-6933

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 環境汚染物質を分解する酵素遺伝子の土壌からの直接的取得と解析2006

    • Author(s)
      小野玲, ら
    • Journal Title

      バイオインダストリー 23(11)

      Pages: 44-49

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ハロアルカンデハロゲナーゼの構造と機能2006

    • Author(s)
      永田裕二, 津田雅孝
    • Journal Title

      J. Environ. Biotechnol. 6

      Pages: 87-92

    • Related Report
      2006 Annual Research Report
  • [Presentation] Burkholderia multivorans ATCC 17616株のFurを介した一酸化窒素(NO)に対する適応機構2009

    • Author(s)
      湯原悟志, ら
    • Organizer
      第3回日本ゲノム微生物学会総会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] 自己伝達性プラスミドの接合伝達過程における転写誘導2009

    • Author(s)
      宮腰昌利, ら
    • Organizer
      第3回日本ゲノム微生物学会総会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] GenomeMatcher : 比較ゲノムソフトウエアとその付属機能2009

    • Author(s)
      大坪嘉行, ら
    • Organizer
      第3回日本ゲノム微生物学会総会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] ナフタレン分解プラスミドNAH7の接合伝達に関与する受容菌側因子の解析2009

    • Author(s)
      井上慧, ら
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] Agrobacterium tumefaciens C58株由来の新規ハロアルカンデハロゲナーゼDatAの解析2009

    • Author(s)
      石塚真祐子, ら
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] Burkholderia multivorans ATCC 17616の愛媛土壌特異的発現遺伝子の鹿島台土壌における発現誘導性の検討2009

    • Author(s)
      山本泰弘, ら
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] IncP-7群プラスミドの分配機構による宿主特異性2009

    • Author(s)
      宮腰昌利, ら
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] 有機塩素系殺虫剤gamma-HCHモデル汚染土壌由来のHCH分解菌の単離と解析2009

    • Author(s)
      府中玄樹, ら
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] 芳香族化合物による土壌の汚染化に対する土壌細菌集団の応答2009

    • Author(s)
      遠藤諒, ら
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] Sphingomonas sp. MM-1株のgamma-HCH分解関連プラスミドの構造解析2009

    • Author(s)
      田端理朗, ら
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] Burkholderia multivorans ATCC 17616株のカタボライト調節における抑制基質認識メカニズムの解明2009

    • Author(s)
      鈴木信太郎, ら
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] 比較ゲノムソフトウエアGenomeMatcherとその付属機能2009

    • Author(s)
      大坪嘉行, ら
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] Pseudoalteromonas atlantica AR06株の産生するアルギン酸リアーゼAlyAの解析2009

    • Author(s)
      松嶋良次, ら
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] Molecular genetic approaches for elucidating adaptive strategies of bacteria in soil2008

    • Author(s)
      Nagata, Y., Y. Ohtsubo, and M. Tsuda
    • Organizer
      ISSM satellite symposium Environmental genomics
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2008-11-15
    • Related Report
      2008 Final Research Report
  • [Presentation] Molecular genetic approaches for elucidating adaptive strategies of bacteria in soil2008

    • Author(s)
      Y. Nagata, et.al.
    • Organizer
      ISSM satellite symposium "Environmental aenomics"
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2008-11-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] Adaptation and evolution of bacteria in naturalenvironments2008

    • Author(s)
      Nagata, Y., Y. Ohtsubo, and M. Tsuda
    • Organizer
      Memorial Symposium of the 24th International Prize for Biology -Ecology for the Changing World
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] 分子遺伝学的手法による細菌の土壌環境適応戦略の解明2008

    • Author(s)
      大坪嘉行, 西山衣里, 宮腰昌利, 永旦裕二, 津田雅孝
    • Organizer
      2008年度日本生物工学会大会シンポジウム
    • Place of Presentation
      仙台
    • Related Report
      2008 Final Research Report
  • [Presentation] 重染色体性のBurkholderia multivoransのゲノム構造と土壌でのゲノム情報発現日本土壌微生物学会2008

    • Author(s)
      津田雅孝多
    • Organizer
      2008年度大会シンポジウム
    • Place of Presentation
      静岡
    • Related Report
      2008 Final Research Report
  • [Presentation] Characterization of plasmids from gamma-hexachlorocyclohexane-degrading bacterium Sphingomonas sp. MM-12008

    • Author(s)
      M. Tabata, et.al.
    • Organizer
      International Plasmid Biology Conference 2008
    • Place of Presentation
      Gdansk, Poland
    • Related Report
      2008 Annual Research Report
  • [Presentation] Reconstruction of transcription networks by the conjugative transfer of IncP plasmids2008

    • Author(s)
      M. Miyakoshi, et.al.
    • Organizer
      International Plasmid Biology Conference 2008
    • Place of Presentation
      Gdansk, Poland
    • Related Report
      2008 Annual Research Report
  • [Presentation] Characterization of the traD operon in Incp-9 plasmid NAH7 : a host-range modifier in conjugative transfer2008

    • Author(s)
      R. Miyazaki, et.al.
    • Organizer
      International Plasmid Biology Conference 2008
    • Place of Presentation
      Gdansk, Poland
    • Related Report
      2008 Annual Research Report
  • [Presentation] Analysis of alginate lyase and its gene from Pseudoalteromonas atlantica AR062008

    • Author(s)
      R. Matsushima, et.al.
    • Organizer
      5th World Fisheries Congress
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2008 Annual Research Report
  • [Presentation] Adaptation and evolution of bacteria in natural environments2008

    • Author(s)
      Y. Nagata, et.al.
    • Organizer
      Memorial Symposium of the 24th International Prize for Biology-Ecology for the Changing World
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2008 Annual Research Report
  • [Presentation] 分子遺伝学的手法による細菌の土壌環境適応戦略の解明2008

    • Author(s)
      大坪嘉行, ら
    • Organizer
      日本生物工学会大会シンポジウム
    • Place of Presentation
      仙台
    • Related Report
      2008 Annual Research Report
  • [Presentation] GenomeMatcher比較ゲノム用ソフトウエア2008

    • Author(s)
      大坪嘉行, ら
    • Organizer
      第2回日本ゲノム微生物学会年会
    • Place of Presentation
      大阪大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] Pseudomonas putida KT2440株のゲノミックアイランドに存在するparAホモログのIncP-7群プラスミドによる転写誘導2008

    • Author(s)
      宮腰昌利, ら
    • Organizer
      第2回日本ゲノム微生物学会年会
    • Place of Presentation
      大阪大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] プラスミドの接合伝達に関与する受容菌側因子の同定2008

    • Author(s)
      宮崎亮, ら
    • Organizer
      第2回日本ゲノム微生物学会年会
    • Place of Presentation
      大阪大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] スフィンゴモナス属類縁細菌群の多彩な難分解性物質分解能を支えるゲノム的基盤2008

    • Author(s)
      永田 裕二, ら
    • Organizer
      第2回日本ゲノム微生物学会年会
    • Place of Presentation
      大阪大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] スロット部の改変により1,2,3-trichloropropane分解活性を強化した変異型ハロアルカン脱ハロゲン酵素DhaAの解析2008

    • Author(s)
      M. Pavlova, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] gamma-HCH分解細菌Sphingomonas sp. MM-1株のgamma-HCH分解関連プラスミドの解析2008

    • Author(s)
      田端理朗, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] モデル土壌の有機塩素系殺虫剤gamma-HCHによる人工的汚染化に関する研究2008

    • Author(s)
      府中玄樹, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] Signature-tagged mutagenesisによるBurkholderia multivorans ATCC17616株の土壌環境生残必須遺伝子の同定2008

    • Author(s)
      宮腰昌利, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] ナフタレン分解プラスミドNAH7の接合伝達に関与する受容菌側因子の同定2008

    • Author(s)
      宮崎亮, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] 2種の土壌細菌においてカタボライト調節をおこす抑制基質の解析2008

    • Author(s)
      石塚真祐子, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] ハロアルカン脱ハロゲン酵素LinBの数アミノ酸の分岐を促した環境要因の探索2008

    • Author(s)
      伊藤通浩, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] 比較ゲノム解析用ソフトウエアGenomeMatcherの開発2008

    • Author(s)
      大坪嘉行, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] Pseudomonas putida mt-2由来のTn3型トランスポゾンTn4651のcointegrate-resolutionに関与するシス・エレメントの解析2008

    • Author(s)
      矢野大和, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] スフィンゴビウム属細菌の細胞外膜安定性に関わるABCトランスポーター2008

    • Author(s)
      遠藤諒, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] Pseudomonas putidaHS1株由来のトルエン分解プラスミドpDK1のゲノム解析2008

    • Author(s)
      長田和也, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] 生態系での細菌ゲノム情報発現遺伝学的手法を用いた発現遺伝子群の探索極限環境微生物学会2007

    • Author(s)
      津田雅孝, 西山依里, 宮腰昌利, 永田裕二, 大坪嘉行
    • Organizer
      第8回シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2007-07-03
    • Related Report
      2008 Final Research Report
  • [Presentation] 生態系での細菌ゲノム情報発現-遺伝学的手法を用いた発現遺伝子群の探索-2007

    • Author(s)
      津田雅孝, ら
    • Organizer
      極限環境微生物学会第8回シンポジウム
    • Place of Presentation
      東京工業大学すずかけ台キャンパス
    • Year and Date
      2007-07-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] 機能発現に基づく環境汚染物質分解酵素遺伝子の生態系からの直接的取得と解析2007

    • Author(s)
      津田雅孝, 小野玲, 宮崎亮, 府中玄樹, 永田裕二
    • Organizer
      第59回日本生物工学会大会シンポジウム「メタゲノム研究と環境バイオテクノロジー」
    • Place of Presentation
      広島大学東広島キャンパス総合科学部
    • Related Report
      2008 Final Research Report
  • [Presentation] 培養非依存的手法による土壌環境からの環境汚染物質分解酵素遺伝子の取得2007

    • Author(s)
      永田裕二、小野玲、宮崎亮、府中玄樹、大坪嘉行、津田雅孝
    • Organizer
      日本微生物生態学会第23回大会シンポジウムI : 微生物生態学における環境ゲノミクス
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2008 Final Research Report
  • [Presentation] 環境での微生物ゲノム情報発現2007年度日本農芸化学会中四国・西日本支部合同大会2007

    • Author(s)
      津田雅孝
    • Organizer
      シンポジウム「ゲノムからの旅立ち」
    • Place of Presentation
      山口
    • Related Report
      2008 Final Research Report
  • [Presentation] Characterization of the traD gene cluster involved in the conjugative transfer of naphthalene-catabolic plasmid NAH72007

    • Author(s)
      Miyazaki, R., Y. Ohtsubo, Y. Nagata, M.Tsuda
    • Organizer
      ASM Conference on Pseudomonas 2007 Seattle
    • Place of Presentation
      Washington, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Haloalkane Dehalogenases Possess Two Different Structural Bases for Their Enantioselectivity2007

    • Author(s)
      Prokop, Z., Y. Sato, T. Mozga, P. Jerabek,R. Natsume, J. Florian, M. Tsuda, Y. Nagata,T. Senda, and J. Damborsky
    • Organizer
      BIOTRANS 2007 - 8th International Symposium on Biocatalysis and Biotransformations Oviedo
    • Place of Presentation
      Spain
    • Related Report
      2008 Final Research Report
  • [Presentation] Functional screening of genes for aromatic ring-hydroxylating oxygenases from soil metagenomic libraries Metagenomics 2007 San Diego2007

    • Author(s)
      Ono, A., Y. Ohtsubo, Y. Nagata, M. Tsuda
    • Organizer
      California
    • Place of Presentation
      USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Haloalkane Dehalogenases Possess Two Different Structural Bases for Their Enantioselectivity2007

    • Author(s)
      Z. Prokop, ら
    • Organizer
      BIOTRANS 2007-8th International Symposium on Biocatalysis and Biotransformations
    • Place of Presentation
      Oviedo, Spain
    • Related Report
      2007 Annual Research Report
  • [Presentation] Functional screening of genes for aromatic ring-hydroxylating oxygenases from soil metagenomic libraries2007

    • Author(s)
      A. Ono, ら
    • Organizer
      Metagenomics 2007
    • Place of Presentation
      San Diego, California, USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] Characterization of the traD gene cluster involved in the conjugative transfer of naphthalene-catabolic plasmid NAH72007

    • Author(s)
      R. Miyazaki, ら
    • Organizer
      ASM Conference on Pseudomonas 2007
    • Place of Presentation
      Seattle, Washington, USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] 培養非依存的手法による土壌環境からの環境汚染物質分解酵素遺伝子の取得2007

    • Author(s)
      永田裕二, ら
    • Organizer
      日本微生物生態学会第23回大会シンポジウム
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] 有機塩素系殺虫剤gamma-HCHによる土壌の試験的汚染化に関する研究2007

    • Author(s)
      府中玄樹, ら
    • Organizer
      日本微生物生態学会第23回大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] Signature-tagged mutagenesis法によるBurkholderia multivorans ATCC17616株の土壌環境必須遺伝子の同定2007

    • Author(s)
      宮腰昌利, ら
    • Organizer
      第23回日本微生物生態学会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] カイメン共在細菌メタゲノム由来のメタ開裂酵素遺伝子の取得と解析2007

    • Author(s)
      渡邊洋平, ら
    • Organizer
      第23回日本微生物生態学会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] メタゲノム的手法による土壌からの環境汚染物質分解酵素遺伝子の取得と解析2007

    • Author(s)
      小野玲, ら
    • Organizer
      第23回日本微生物生態学会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] 機能発現に基づく環境汚染物質分解酵素遺伝子の生態系からの直接的取得と解析2007

    • Author(s)
      津田雅孝, ら
    • Organizer
      第59回日本生物工学会大会シンポジウム
    • Place of Presentation
      広島大学東広島キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] カイメン共在細菌メタゲノム中のハロアルカン脱ハロゲン酵素遺伝子相同配列の解析2007

    • Author(s)
      荒井保亮, ら
    • Organizer
      第59回日本生物工学会大会
    • Place of Presentation
      広島大学東広島キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] インドの高濃度HCH汚染根圏土壌から取得されたγ-HCH分解資化細菌Sphingomonas sp. MM-1株のγ-HCH分解関与遺伝子群に関する研究2007

    • Author(s)
      田端理朗, ら
    • Organizer
      第59回日本生物工学会大会
    • Place of Presentation
      広島大学東広島キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] スフィンゴモナス属細菌の細胞膜の安定性に関わる新規ABCトランスポーター2007

    • Author(s)
      遠藤諒, ら
    • Organizer
      第30回分子生物学会年会・第80回生化学会大会合同大会
    • Place of Presentation
      横浜市 パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] 細菌由来の脱ハロゲン酵素の構造一機能相関と応用への展望2006

    • Author(s)
      永田裕二
    • Organizer
      平成18年度日本生物工学会北日本支部七タシンポジウム「新しいバイオ技術の創製を目指して」
    • Place of Presentation
      仙台
    • Year and Date
      2006-08-05
    • Related Report
      2008 Final Research Report
  • [Presentation] Novel ABC-type transporter genes essential for the γ-hexachlorocyclohexane utilization in Sphingobium japonicum UT262006

    • Author(s)
      Endo, R., Y. Ohtsubo, M. Tsuda, and Y.Nagata
    • Organizer
      International Symposium on Environmental Biotechnology
    • Place of Presentation
      Leipzig, Germany
    • Related Report
      2008 Final Research Report
  • [Presentation] Direct isolation of genes and a plasmid for the degradation of γ-hexachlorocyclohexane from contaminated soil2006

    • Author(s)
      Nagata, Y., M. Ito, R. Miyazaki, G. Fuchu, Y. Ohtsubo, and M. Tsuda
    • Organizer
      International Symposium on Environmental Biotechnology
    • Place of Presentation
      Leipzig, Germany
    • Related Report
      2008 Final Research Report
  • [Book] 難培養性細菌も研究対象とするメタゲノム解析「バイオフィルムの基礎と制御」2008

    • Author(s)
      永田裕二、津田雅孝
    • Publisher
      103-111株式会社エヌ・ティー・エス
    • Related Report
      2008 Final Research Report
  • [Book] バイオフィルムの基礎と制御(部分執筆)2008

    • Author(s)
      永田裕二、津田雅孝
    • Total Pages
      399
    • Publisher
      株式会社エヌ・ティー・エス
    • Related Report
      2007 Annual Research Report
  • [Book] 「比較ゲノム学から読み解く生命システム-基本概念から最新ゲノム情報まで(細胞工学別冊)」2007

    • Author(s)
      津田雅孝、永田裕二、大坪嘉行
    • Publisher
      土壌環境細菌の比較ゲノム
    • Related Report
      2008 Final Research Report
  • [Book] 比較ゲノム学から読み解く生命システム〜基本概念から最新ゲノム情報まで(部分執筆)2007

    • Author(s)
      津田雅孝, ら
    • Total Pages
      196
    • Publisher
      秀潤社
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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