Studies on breeding technology using natural clonal and polyploid fish as genetic resources

• Project/Area Number: 18380108
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: ARAI Katsutoshi Hokkaido University, 大学院・水産科学研究院, 教授 (00137902)
• Co-Investigator(Kenkyū-buntansha): ABE Shuichi 北海道大学, 大学院・水産科学研究院, 教授 (80125278) ; YAMAHA Etsuro 北海道大学, 北方生物圏フィールド科学センター, 教授 (60191376) ; MURAKAMI Masaru 麻布大学, 獣医学部, 教授 (80271360)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,490,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥2,490,000); Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2007: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2006: ¥6,700,000 (Direct Cost: ¥6,700,000)
• Keywords: クローン / 倍数体 / 雌性発生 / 雄性発生 / 性転換 / 減数分裂 / 単為発生 / 非還元配偶子

Research Abstract

ドジョウは両性生殖で繁殖するのが普通であるが、通常の減数分裂によらず非還元二倍体の卵を産み、かつ、これらの卵が雌性発生により父親の遺伝的関与なく発生するクローン系統、および偶然の精子核取り込みにより生じる自然三倍体系統が野生集団に見られる。本研究は、このような特殊な生殖の機構と起源を明らかにするとともに、このような変異体を遺伝資源(育種素材)として、交雑・染色体操作と組み合わせることにより育種技術開発を行った。

Research Products

• [Journal Article] ドジョウの倍数性とクローン、それらの特殊な生殖様式 (2009)
  • Author(s): 荒井克俊
  • Journal Title: 動物遺伝育種 37(1)(印刷中)
  • NAID: 10024932803
  • Peer Reviewed
• [Journal Article] A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species (2009)
  • Author(s): Yasui GS, Arias-Rodriguez L, Fujimoto T, Arai K
  • Journal Title: Animal Reproduction Science (DOI 10.1016/j.anireprosci.) (in press)
  • NAID: 120001788328
  • Peer Reviewed
• [Journal Article] Chromosome doubling in early spermatogonia produces diploid spermatozoa in a natural clonal fish (2009)
  • Author(s): Yoshikawa H, Morishima K, Fujimoto T, Saito T, Kobayashi T, Yamaha E, Arai K
  • Journal Title: Biology of Reproduction (DOI10.1095/biolreprod.108.075150) 80 Pages: 973-979
  • Peer Reviewed
• [Journal Article] Inter-populational difference in microsatellite-centromere mapdistances in the loach, Misgurnus anguillicaudatus (2009)
  • Author(s): Arias-Rodriguez L, Morishima K, Arai K
  • Journal Title: Ichthyol Res (DOI 10.1007/s10228-008-0077-7) 56 Pages: 126-132
  • Peer Reviewed
• [Journal Article] Disruption of normal meiosis in artificial inter-populational hybrid females of Misgurnus loach (2009)
  • Author(s): Arias-Rodriguez L, Yasui GS, Arai K
  • Journal Title: Genetica (DOI 10.1007/s10709-008-9299x) 136 Pages: 49-56
  • NAID: 120002083305
  • Peer Reviewed
• [Journal Article] Simple and inexpensive method for cryopreservation of fish sperm combining straw and powdered dry ice (2008)
  • Author(s): Yasui GS, Arias-Rodriguez L, Fujimoto T, Arai K
  • Journal Title: CryoLetters 29(5) Pages: 383-390
  • NAID: 120001492944
  • Peer Reviewed
• [Journal Article] Genetic and reproductive potential of spermatozoa of diploid and triploid males obtained from interspecific hybridization of Misgurnus anguillicaudatus female and M.mizolepis male (2008)
  • Author(s): Fujimoto T, Yasui GS, Yoshikawa H, Yamaha E, Arai K
  • Journal Title: J Appl Ichthyol (DOI 10.1111/j.1439-0426.2008.01131.x) 24 Pages: 430-437
  • Peer Reviewed
• [Journal Article] Ploidy manipulation using diploid sperm in the loach, Misgurnus anguillicaudatus : a review (2008)
  • Author(s): Yoshikawa H, Morishima K, Fujimoto T, Arias-Rodriguez L, Yamaha E, Arai K
  • Journal Title: J Appl Ichthyol (DOI 10.1111/j.1439-0426.2008.01129.x) 24 Pages: 410-414
  • NAID: 120001442845
  • Peer Reviewed
• [Journal Article] Meiotic hybridogenesis in triploid Misgurnus loach derived from a clonal lineage (2008)
  • Author(s): Morishima K, Yoshikawa H, Arai K
  • Journal Title: Heredity (DOI 10.1038/hdy.2008.17) 100 Pages: 581-586
  • NAID: 120001054061
  • Peer Reviewed
• [Journal Article] 中国におけるドジョウ倍数体の分布に関する研究 (2008)
  • Author(s): 李雅娟, 印傑, 王嘉博, 袁旭, 魏傑, 孫効文, 荒井克俊
  • Journal Title: 日本水産学会誌 74(2) Pages: 177-182
  • NAID: 110006644795
  • Peer Reviewed
• [Journal Article] Genetic linkage map of the loach Misgurnus anguillicaudatus (2008)
  • Author(s): Morishima K, Nakayama I, Arai K
  • Journal Title: Genetica (DOI 10.1007/s10709-007-9165-2) 132 Pages: 227-241
  • Peer Reviewed
• [Journal Article] Cryptic clonal lineages and genetic diversity in the loach Misgurnus anguillicaudatus (Teleostei : Cobitidae) inferred from nuclear and mitochondrial DNA analyses (2008)
  • Author(s): Morishima K, Nakamura-Shiokawa Y, Bando E, Li YJ, Boron A, Khan MMR, Arai K
  • Journal Title: Genetica (DOI 10.1007/s10709-007-9158-1) 132 Pages: 159-171
  • Peer Reviewed
• [Journal Article] Simple and inexpensive method for cryopreservation of fish sperm combining straw and powdered dry ice (2008)
  • Author(s): Yasui GS, Arias-Rodriguez L. Fujimoto T. Arai K
  • Journal Title: CryoLetters 29 Pages: 383-390
  • NAID: 120001492944
  • Peer Reviewed
• [Journal Article] Genetic and reproductive potential of spermatozoa of diploid and triploid malesobtained from interspecific hybridization of Misgurnus anguillicaudatus female and M. mizolepls male (2008)
  • Author(s): Fujimoto T. Yasui GS, Yoshikawa H, Yamaha E, Arai K
  • Journal Title: Journal of Applied Ichthyology 24 Pages: 430-437
  • Peer Reviewed
• [Journal Article] Ploidy manipulation using diploid sperm in the loach, Alisugurims anguillicaudatus : a review (2008)
  • Author(s): Yoshikawa H, Morishima K, Fujimoto T, Arias-Rodiriguez L, Yamaha E, Arai K
  • Journal Title: Journal of Applied Ichthyology 24 Pages: 410-414
  • Peer Reviewed
• [Journal Article] Meiotic hybridogenesis in triploid Alisgurnus anguillicaudatus derived from a clonal lineage (2008)
  • Author(s): Morishima K, Yoshikawa H, Arai K
  • Journal Title: Heredity 100 Pages: 581-586
  • Peer Reviewed
• [Journal Article] 中国におけるドジョウ倍数体の分布に関する研究 (2008)
  • Author(s): 李雅娟・印傑・王嘉博・袁旭・魏傑・孫効文・荒井克俊
  • Journal Title: 日本水産学会誌 74 Pages: 177-182
  • NAID: 110006644795
  • Peer Reviewed
• [Journal Article] Cryptic clonal lineages and genetic diversity in the loacb Misgurnus anguillicaudatus(Teleostei: Cobitidae)inferred from nuclear and mitochondrial DNA analyses (2008)
  • Author(s): K Morishima, Y Nakamura-Shiokawa, E Bando, YJ Li, A Boron, MMR Khan, K Arai
  • Journal Title: Genetica 132 Pages: 159-171
  • Peer Reviewed
• [Journal Article] Genetic linkage map of the loach Misgurnus anguillicaudatus(Teleostei: Cobitidae) (2008)
  • Author(s): K Morishima, I Nakayama, K Arai
  • Journal Title: Genetica 132 Pages: 227-241
  • Peer Reviewed
• [Journal Article] Evaluation of different doses of UV irradiation to loach eggs for genetic inactivation of the maternal genome (2007)
  • Author(s): Fujimoto T, Sakao S, Yamaha E, Arai K
  • Journal Title: J Exp Zool(DOI 10.1002/jez.398) 307A Pages: 449-462
  • Peer Reviewed
• [Journal Article] Simultaneous formation of haploid, diploid and triploid eggs in diploid-triploid mosaic loaches (2007)
  • Author(s): Yoshikawa H, Morishima K, Fujimoto T, Yamaha E, Arai K
  • Journal Title: J Fish Biology (DOI 10.1111/j.1095-8649.2009.01608.x) 71(Supplement B) Pages: 250-263
  • Peer Reviewed
• [Journal Article] Evaluation of different doses of UV irradiation to loach eggs for genetic inactivation of the maternal genome (2007)
  • Author(s): T Fujimoto, S Sakao, E Yamaha, K Arai
  • Journal Title: Journal of Experimental Zoology Part A 307A Pages: 449-462
  • Peer Reviewed
• [Journal Article] Simultaneous formation of haploid, diploid and triploid eggs in diploid-triploid mosaic loaches (2007)
  • Author(s): H Yoshikawa, K Morishima, T Fujimoto, E Yamaha, K Arai
  • Journal Title: Journal of Fish Biology 71(SuppLB) Pages: 250-263
  • Peer Reviewed
• [Presentation] 外来倍数体ドジョウの起源 (2009)
  • Author(s): 李雅娟, 荒井克俊
  • Organizer: 平成21(2009)年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス, 東京都
  • Year and Date: 2009-03-28
• [Presentation] Molecular cytogenetics of viable and inviable salmonid hybrids (2008)
  • Author(s): Abe S, Fujiwara A, Ito D, Sato K, Yamaguchi M, Zheng L, Kimura S, Yamaha E
  • Organizer: 5^<th> World Fisheries Congress
  • Place of Presentation: Pacifico Yokohama, Yokohama
  • Year and Date: 2008-10-22
• [Presentation] Genetic markers, mapping and manipulation for analyses of mechanisms of atypical reproduction in the loach, Misgurnus anguillicaudatus (2008)
  • Author(s): Arai K, Morishima K, Yoshikawa H, Arias-Rodriguez L, Fujimoto T, Yamaha E
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Pacifico Yokohama, Yokohama
  • Year and Date: 2008-10-22
• [Presentation] Molecular cytogenetics of viable and inviable salmonidhybrids (2008)
  • Author(s): Abe S, Fujiwara A, Ito D, S ato K, Yamaaguchi M, Zheng L, Kimura S, Yamaha E
  • Organizer: 5^<th> Wsorld Fisheries Conference
  • Place of Presentation: Pacifico横浜、横浜市
  • Year and Date: 2008-10-22
• [Presentation] Genetic markers, mapping and manipulation for-analyses of me chanisms of atypical reproduction in the loach, MiSgYIBUS an guillicaudatus (2008)
  • Author(s): Arai K, Morishima K, Yoshikawa H, Arias-Rodriguez I, Fuiimoto T. Yamaha E
  • Organizer: 5^<th> Wsorld Fisheries Conference
  • Place of Presentation: Pacifico横浜、横浜市
  • Year and Date: 2008-10-22
• [Presentation] クローンドジョウの起源 (2008)
  • Author(s): 荒井克俊, 森島輝, 吉川廣幸, 李雅娟
  • Organizer: 日本動物学会北海道支部第54回大会
  • Place of Presentation: 北海道大学学術交流会館, 札幌
  • Year and Date: 2008-08-09
• [Presentation] クローンドジョウの起源 (2008)
  • Author(s): 荒井克俊・森島輝・吉川廣幸・李雅娟
  • Organizer: 日本動物学会北海道支部会第54回大会
  • Place of Presentation: 北海道大学学術交流会館、札幌市
  • Year and Date: 2008-08-09
• [Presentation] Genetic marker, map and manipulation in aquatic species (2008)
  • Author(s): Arai K
  • Organizer: World Aquaculture 2008
  • Place of Presentation: Busan, Korea
  • Year and Date: 2008-05-23
• [Presentation] Genetic marker, map and manipulation in aquatic species (2008)
  • Author(s): Arai K
  • Organizer: World Aquaculture 2008
  • Place of Presentation: プサン・コンベンションセンター、大韓民国釜山市
  • Year and Date: 2008-05-23
• [Presentation] 雑種と異質三倍体は借り腹生産の不妊化宿主として有効か (2008)
  • Author(s): 藤本貴史, Yasui GS, 吉川廣幸, 山羽悦郎, 荒井克俊
  • Organizer: 平成20年度日本水産学会春季大会
  • Place of Presentation: 東海大学海洋学部, 静岡市
  • Year and Date: 2008-03-30
• [Presentation] ドジョウ集団間におけるマーカー-動原体間地図距離の相違 (2008)
  • Author(s): アリアス-ロドリゲスレニン, 森島輝, 荒井克俊
  • Organizer: 平成20年度日本水産学会春季大会
  • Place of Presentation: 東海大学海洋学部, 静岡市
  • Year and Date: 2008-03-28
• [Presentation] Genetic differentiation inferred from sequences of mitochondrial DNA control region of the loach Misgurnus anguillicaudatus in China (2008)
  • Author(s): Li YJ, Sun XW, Yuan X, Li Y, Arai K
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Pacifico Yokohama, Yokohama
• [Presentation] Genetic differentiation inferred from sequences of mitochondrial DNA control region of the loach Misgurnus anguillicaudatus in China (2008)
  • Author(s): Li YJ, Sun XW, Yuan X, Li Y, Arai K
  • Organizer: 5^<th> Wsorld Fisheries Conference
  • Place of Presentation: Pacifico横浜、横浜市
• [Presentation] ドジョウ雌とカラドジョウ雄間の雑種・異質三倍体は不妊か? (2007)
  • Author(s): 藤本貴史・Yasui GS, 吉川廣幸
  • Organizer: 平成19年度日本水産学会秋季大会
  • Place of Presentation: 北海道大学水産学部, 函館市
  • Year and Date: 2007-09-27
• [Presentation] クローン四倍体ドジョウは減数分裂により二倍体精子をつくる (2007)
  • Author(s): 吉川廣幸, 森島輝, 藤本貴史, 荒井克俊
  • Organizer: 平成19年度日本水産学会秋季大会
  • Place of Presentation: 北海道大学水産学部, 函館市
  • Year and Date: 2007-09-27
• [Presentation] ストローとドライアイスを用いた冷却速度制御による精子凍結保存の新方法 (2007)
  • Author(s): Yasui GS, 藤本貴史, 荒井克俊
  • Organizer: 平成19年度日本水産学会秋季大会
  • Place of Presentation: 北海道大学水産学部, 函館市
  • Year and Date: 2007-09-27
• [Presentation] クローン四倍体ドジョウは減数分裂により二倍体精子をつくる (2007)
  • Author(s): (吉川廣幸)吉川廣幸, 他3名
  • Organizer: 平成19年度日本水産学会秋季大会
  • Place of Presentation: 北大、函館市
  • Year and Date: 2007-09-27
• [Presentation] Sperm cryopreservation in the loach new method to control coaling rates with the straw and powdered dry-ice (2007)
  • Author(s): (GS Yasui)GS Yasui, 他2名
  • Organizer: 平成19年度日本水産学会秋季大会
  • Place of Presentation: 北大、函館市
  • Year and Date: 2007-09-27
• [Presentation] ドジョウ雌とカラドジョウ雄間の雑種・異質倍数体は不妊か? (2007)
  • Author(s): (藤本貴史)藤本貴史, 他4名
  • Organizer: 平成19年度日本水産学会秋季大会
  • Place of Presentation: 北大、函館市
  • Year and Date: 2007-09-26
• [Presentation] Genetic and reproductive traits of spermatozoa from diploid and triploid hybrid males between Misgurnus anguillicaudatus female and M. mizolepis male. (2007)
  • Author(s): (藤本貴史)T Fujimoto, GS Yasui, H Yoshikawa. E Yamaha, K Arai
  • Organizer: The 1st International Workshop on the Biology of Fish Sperm
  • Place of Presentation: Vodnany, Czechチェコ
  • Year and Date: 2007-08-30
• [Presentation] Genetic and reproductive potential of spermatozoa of diploid and triploid males obtained from interspecific hybridization of Misgurnus anguillicaudatus female and M.mizolepis male (2007)
  • Author(s): Fujimoto T, Yasui GS, Yoshikawa H, Yamaha E, Arai K
  • Organizer: The 1st International Workshop on the Biology of Fish Sperm
  • Place of Presentation: Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech
  • Year and Date: 2007-08-29
• [Presentation] Ploidy manipulation using diploid sperm in the loach, Misgurnus anguillicaudatus : a review (2007)
  • Author(s): Yoshikawa H, Morishima K, Fujimoto T, Arias-Rodriguez L, Yamaha E, Arai K
  • Organizer: The 1^<st> International Workshop on the Biology of Fish Sperm
  • Place of Presentation: Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech
  • Year and Date: 2007-08-29
• [Presentation] Ploidy manipulation using diploid sperm in the loach, Misgurnus anguillicaudatus: A review (2007)
  • Author(s): (荒井克俊)H Yoshikawa、 K Morishima, T Fujimoto, 他3名
  • Organizer: The 1st International Workshop on the Biology of Fish Sperm
  • Place of Presentation: Vodnany, Czechチェコ
  • Year and Date: 2007-08-29
• [Presentation] 自然クローンドジョウの非還元卵形成とゲノムセット構成の関係 (2007)
  • Author(s): 森島輝, 荒井克俊
  • Organizer: 平成19年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学、東京
  • Year and Date: 2007-03-28
• [Presentation] ドジョウの地域集団間雑種第一代に見られた非還元2n卵形成 (2007)
  • Author(s): アリアスーロドリゲスレニン, 荒井克俊
  • Organizer: 平成19年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学、東京
  • Year and Date: 2007-03-28
• [Presentation] ドジョウの地域集団間雑種はごく少量の1nおよび2n精子をつくる (2007)
  • Author(s): アリアスーロドリゲスレニン, 楠田聡, 荒井克俊
  • Organizer: 平成19年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学、東京
  • Year and Date: 2007-03-28
• [Presentation] 借り腹生産における三倍体の不妊宿主としての有効性 (2007)
  • Author(s): 藤本貴史, 吉川廣幸, 森島輝, 山羽悦郎, 荒井克俊
  • Organizer: 平成19年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学、東京
  • Year and Date: 2007-03-28
• [Presentation] Recent progress in reproductive biology and genetics of Misgurnus loaches (2006)
  • Author(s): Morishima K, Fujimoto T, Yoshikawa H, Arai K
  • Organizer: 3^<rd> International Conference, Loaches of the Genus Cobitis and Related Genera
  • Place of Presentation: Sibenik, Croatia
  • Year and Date: 2006-09-25
• [Presentation] A genetic map of the loach Misgurnus anguillicaudatus (Teleostei : Cobitidae) (2006)
  • Author(s): Morishima K, Arias-Rodriguez L, Nakayama I, Arai K
  • Organizer: 9^<th> International Symposium Genetics in Aquaculture
  • Place of Presentation: Montpellier, France
• [Presentation] Hybridogenesis-like oogenesis in triploid females derived from the clone lineage of Misgurnus loach
  • Author(s): Morishima K, Oshima K, Arai K
  • Organizer: 9^<th> International Symposium Genetics in Aquaculture
  • Place of Presentation: Montpellier, France