Studies on the cellular mechanism of collagen fibril alignment in the regenerating scales of goldfish : toward the bioinspired fabrication of artificial cornea

• Project/Area Number: 18380109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: TAKAGI Yasuaki Hokkaido University, 大学院・水産科学研究院, 教授 (10212002)
• Co-Investigator(Kenkyū-buntansha): URA Kazuhiro 北海道大学, 大学院・水産科学研究院, 助教 (90360940) ; IKOMA Toshiyuki 独立行政法人物質・材料研究機構, 生体材料研究センター (20370306)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥14,520,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥1,620,000); Fiscal Year 2008: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2007: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2006: ¥7,500,000 (Direct Cost: ¥7,500,000)
• Keywords: コラーゲン / 配列制御 / 変性温度 / 組織再生医療用材料 / 角膜再生 / 骨再生 / ウロコ / キンギョ / 組織再生医療 / 硬骨魚類

Research Abstract

ウロコのコラーゲンを用いて再生医療用人工基質の合成をめざして、生物学と材料科学の両面から研究を推進した。生物学的アプローチにより鱗形成細胞分化の分子機構、コラーゲン配向を制御する候補分子、組織ごとのコラーゲンα鎖組成を明らかにした。また、材料科学的アプローチにより、ブタ及びティラピアコラーゲン線維配向ゲルを高磁場内で実現するとともに、湿潤環境下による乾燥により10~20wt%の高密度化に成功した。

Research Products

• [Journal Article] ナノサーチ顕微鏡による細胞外マトリックスの観察 (2008)
  • Author(s): 小暮亮雅、粉川良平、藤井岳直、紋川亮、生駒俊之、竹村太郎、花形信孝、田中順三
  • Journal Title: 島津評論 64(3・4) Pages: 131-136
• [Journal Article] Scale regeneration and calcification in the goldfish Carassius auratus : quantitative and morphological process (2007)
  • Author(s): Ohira Y, Shimizu M, Ura K, Takagi Y
  • Journal Title: Fisheries Science 73 Pages: 46-54
  • NAID: 10018292208
  • Peer Reviewed
• [Journal Article] Teleost fish scales : a unique biological model for the fabrication of materials for corneal stroma regeneration (2007)
  • Author(s): Takagi Y, Ura K
  • Journal Title: Journal of Nanoscience and Nanotechnology 7 Pages: 757-762
  • Peer Reviewed
• [Journal Article] 魚類のウロコに認められる角膜様のコラーゲン配列とその形成メカニズムの解明 (2007)
  • Author(s): 都木靖彰
  • Journal Title: マテリアルインテグレーション 20(11) Pages: 20-26
• [Journal Article] Three-dimensional porous hydroxyapatite/collagen composite with rubber-like elasticity (2007)
  • Author(s): Yunoki S, Ikoma T, Monkawa A, Marukawa E, Sotome S, Shinomiya K, Tanaka J
  • Journal Title: Journal of Biomaterials Science-Polymer Edition 18(4) Pages: 393-409
  • Peer Reviewed
• [Journal Article] Phenotype and gene expression pattern of osteoblast-like cells cultured on polystyrene and hydroxyapatite with pre-adsorbed type I collagen (2007)
  • Author(s): Hanagata N, Takemura T, Monkawa A, Ikoma T, Tanaka J
  • Journal Title: Journal of Biomedical Materials, Part A 83A(2) Pages: 362-371
  • Peer Reviewed
• [Journal Article] ホメオスタシスを調整する生体無機材料 (2007)
  • Author(s): 生駒俊之、花方信孝、利根川亨、吉岡朋彦、田中順三
  • Journal Title: マテリアルインテグレーション 20(11) Pages: 14-19
• [Journal Article] Scale regeneration and calcification in the goldfish Carassius auratus : quantitative and morphological process (2007)
  • Author(s): Ohira, Y., M. Shimizu, K. Ura and Y. Takagi
  • Journal Title: Fish. Sci. 73 Pages: 46-54
  • NAID: 10018292208
  • Peer Reviewed
• [Journal Article] Teleost fish scales : a unique biological model for the fabrication of materials for corneal stroma regeneration (2007)
  • Author(s): Takagi, Y. and K. Ura
  • Journal Title: J. Nanosci. Nanotech. 7 Pages: 757-762
  • Peer Reviewed
• [Journal Article] 硬骨魚類の鱗の石灰化機構を探る (2007)
  • Author(s): 小川展弘・都木靖彰
  • Journal Title: バイオインダストリー 24(12) Pages: 47-55
• [Journal Article] 硬骨魚類の鱗の石灰化機構を探る. (2007)
  • Author(s): 小川展弘・都木靖彰
  • Journal Title: バイオインダストリー 24 Pages: 47-55
• [Journal Article] 魚類のウロコに認められる角膜様のコラーゲン配列とその形成メカニズムの解明 (2007)
  • Author(s): 都木靖彰
  • Journal Title: マテリアルインテグレーション 20 Pages: 20-26
• [Journal Article] Scale regeneration and calcification in the goldfish Carassius auratus : quantitative and morphological process. 73 : 46-54. (2007)
  • Author(s): Ohira, Y., M.Shimizu, K.Ura, Y.Takagi
  • Journal Title: Fisheries Science 73 Pages: 46-54
• [Journal Article] Teleost fish scales : a unique biological model for the fabrication of materials for corneal stroma regeneration. (2007)
  • Author(s): Takagi, Y., K. Ura
  • Journal Title: Journal of Nanoscience and Nanotechnology 7 Pages: 757-762
• [Journal Article] A dewetting process to nano-pattern collagen on hydroxyapatite (2006)
  • Author(s): Monkawa A, Ikoma T, Yunoki S, Ohta K, Tanaka J
  • Journal Title: Materials Letters 60(29-30) Pages: 3647-3650
  • Peer Reviewed
• [Journal Article] うろこコラーゲンを用いた角膜再建材料への展開 (2006)
  • Author(s): 生駒俊之、柚木俊二、田中順三
  • Journal Title: 高分子7月号 55(7) Pages: 506-506
  • NAID: 10017616825
  • Peer Reviewed
• [Journal Article] In vivo biological responses and bioresorption of tilapia scale collagen as a potential biomaterial
  • Author(s): Sugiura H, Yunoki S, Kondo E, Ikoma T, Tanaka J, Yasuda K
  • Journal Title: Journal of Biomaterials Science-Polymer Edition (in press)
  • Peer Reviewed
• [Presentation] キンギョの鱗再生機構の研究-1鱗再生初期における増殖細胞の分布 (2009)
  • Author(s): 都木靖彰
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学, 東京
  • Year and Date: 2009-03-29
• [Presentation] キンギョの鱗再生機構の研究-2鱗移植実験による鱗再生開始要因に関する研究 (2009)
  • Author(s): 下田健吾
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学, 東京
  • Year and Date: 2009-03-29
• [Presentation] キンギョの鱗再生機構の研究-3鱗形成細胞培養系の確立とその性状解析 (2009)
  • Author(s): 赤路佐希子
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学, 東京
  • Year and Date: 2009-03-29
• [Presentation] キンギョの鱗再生機構の研究-4線維層板を形成する非コラーゲン性タンパク質の性状解析 (2009)
  • Author(s): 小松典彦
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学, 東京
  • Year and Date: 2009-03-29
• [Presentation] キンギョのウロコ再生過程で相対含有量が変動するEDTA可溶性タンパク質 (2008)
  • Author(s): 浅井理沙
  • Organizer: 第3回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京大学弥生講堂, 東京
  • Year and Date: 2008-12-13
• [Presentation] 再生初期のウロコの石灰化は血液由来のHDLによって誘導される (2008)
  • Author(s): 小川展弘
  • Organizer: 第3回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京大学弥生講堂, 東京
  • Year and Date: 2008-12-13
• [Presentation] Expression pattern of Runx2 and SPARC during scale regeneration in goldfish (2008)
  • Author(s): Iimura, K, Tohse H, Ura K, Takagi Y
  • Organizer: The 21st Century COE program 7^<th> International Symposium "Innovative Marine Science for Tree Es, Edibles, Environment and Education in 21st Century"
  • Place of Presentation: Conference Hall, Hokkaido University Sapporo Campus, Sapporo, Japan
  • Year and Date: 2008-11-17
• [Presentation] Expression pattern of Runx2 and SPARC during scale regeneration in goldfish, November 2008, Poster presentation (2008)
  • Author(s): Iimura, K.
  • Organizer: The 21st Century COE program 7^<th> International Symposium "Innovative Marine Science for Trek Es Edibles,Environment and Education in 21st Century"
  • Place of Presentation: Conference Hall, Hokkaido University Sapporo Campus, Sapporo, Japan
  • Year and Date: 2008-11-17
• [Presentation] High density lipoprotein is incorporated into the matrix of fish scale and may act as mineralization inducer (2008)
  • Author(s): Ogawa, N.
  • Organizer: The 21st Century COE program 7^<th> International Symposium "Innovative Marine Science for free Es, Edibles, Environment and Education in 21st Century"
  • Place of Presentation: Conference Hall, Hokkaido University Sapporo Campus, Sapporo, Japan
  • Year and Date: 2008-11-17
• [Presentation] (2008)Plasma high density lipoprotein is incorporated into the matrix of fish scale and may act as mineral ization inducer (2008)
  • Author(s): Ogawa, N., H. Tohse, K. Ura and Y. Takagi
  • Organizer: Gordon Research Conference on Biomineralization
  • Place of Presentation: Colby-Sawyer College, New London, NH, USA.(Poster presentation)
  • Year and Date: 2008-08-11
• [Presentation] Plasma high density lipoprotein is incorporated into the matrix of fish scale and may act as mineralization inducer (2008)
  • Author(s): Ogawa, N.
  • Organizer: Gordon Research Conference on Biomineralization
  • Place of Presentation: Colby-Sawyer College, New London, NH, USA
  • Year and Date: 2008-08-11
• [Presentation] 鱗再生過程における骨芽細胞分化因子および骨基質タンパク質の発現解析 (2007)
  • Author(s): 飯村九林・当瀬秀和・浦和寛・都木靖彰
  • Organizer: 第2回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京大学弥生講堂,東京
  • Year and Date: 2007-12-01
• [Presentation] 人為的石灰化誘導法による再生鱗の石灰化過程の解析 (2007)
  • Author(s): 小川展弘・浦和寛・都木靖彰
  • Organizer: 第2回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京大学弥生講堂,東京
  • Year and Date: 2007-12-01
• [Presentation] 鱗の再生初期の石灰化は高密度脂質タンパク質により誘導される (2007)
  • Author(s): 小川展弘・当瀬秀和・浦和寛・都木靖彰
  • Organizer: 第2回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京大学弥生講堂,東京
  • Year and Date: 2007-12-01
• [Presentation] 魚の鱗はバイオミネラリゼーション研究のユニークなモデルである (2007)
  • Author(s): 都木靖彰・大平康晴・小川展弘・飯村九林・当瀬秀和・浦和寛
  • Organizer: 第2回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京大学弥生講堂,東京
  • Year and Date: 2007-12-01
• [Presentation] Teleost fish scale is a unique model for studying regeneration of dermal skeletons, Gordon Research Conference (2007)
  • Author(s): Takagi Y, Ohira Y, Ogawa N, Iimura K, Tohse H, Ura K
  • Organizer: Gordon Research Conference "Bones and Teeth,"
  • Place of Presentation: University of New England, Biddford, ME, USA
  • Year and Date: 2007-07-15
• [Presentation] mRNA expression patterns of BMP2, Runx2 and SPARC during scale regeneration in goldfish (2007)
  • Author(s): Iimura K, Tohse H, Ura K, Takagi Y
  • Organizer: Gordon Research Conference "Bones and Teeth,"
  • Place of Presentation: University of New England, Biddford, ME, USA
  • Year and Date: 2007-07-15
• [Presentation] (2007)Screening and characterization of calcification-regulating proteins in the fish scale, a dermal skeleton similar to mammalian bone/dentin (2007)
  • Author(s): Ogawa, N., H. Tohse, K. Ura and Y. Takagi
  • Organizer: Gordon Research Conference "Bones and Teeth"
  • Place of Presentation: University of New England, Biddford, ME, USA(Poster presentation)
• [Presentation] (2007)mRNA expression patterns of BMP2, Runx2 and SPARC during scale regeneration in goldfish (2007)
  • Author(s): Iimura, K., H. Tohse, K. Ura and Y. Takagi
  • Organizer: Gordon Research Conference "Bones and Teeth"
  • Place of Presentation: University of New England, Biddford, ME, USA(Poster presentation)
• [Presentation] (2007)Teleost fish scale is a unique model for studying regeneration of dermal skeletons (2007)
  • Author(s): Takagi, Y., Y. Ohira, N. Ogawa, K. Iimura, H. Tohse and K. Ura
  • Organizer: Gordon Research Conference"Bones and Teeth"
  • Place of Presentation: University of New England, Biddford, ME, USA(Poster presentation)
• [Presentation] Screening and characterization of calcification-regulating proteins in the fish scale, a dermal skeleton similar to mammalianbone/dentin. (2007)
  • Author(s): Ogawa, N., H. Tohse, K. Ura and Y. Takagi
  • Organizer: Gordon Research Conference "Bones and Teeth"
  • Place of Presentation: University of New England, Biddford, ME
• [Presentation] mRNA expression patterns of BMP2, Runx2 and SPARC during scale regeneration in goldfish. (2007)
  • Author(s): Iimura, K., H. Tohse, K. Ura and Y. Takagi
  • Organizer: Gordon Research Conference "Bones and Teeth"
  • Place of Presentation: University of New England, Biddford, ME
• [Presentation] Teleost fish scale is a unique model for studying regeneration of dermal skeletons. (2007)
  • Author(s): Takagi, Y., Y. Ohira, N. Ogawa, K. Iimura, H. Tohse and K. Ura
  • Organizer: Gordon Research Conference "Bones and Teeth"
  • Place of Presentation: University of New England, Biddford, ME
• [Presentation] キンギョのBMP2, Runx2およびSPARC mRNAの鱗再生過程における発現解析 (2007)
  • Author(s): 飯村九林・浦和寛・都木靖彰
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前大学文京町キャンパス,弘前
• [Presentation] 鱗再生過程における骨芽細胞分化因子および骨基質タンパク質の発現解析 (2007)
  • Author(s): 飯村九林・浦和寛・都木靖彰
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 北海道大学水産学部,函館
• [Presentation] 魚類の再生鱗における石灰化制御タンパク質の同定 (2007)
  • Author(s): 小川展弘・当瀬秀和・浦和寛・都木靖彰
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 北海道大学水産学部,函館
• [Presentation] Hard tissue research and application in aquatic animals (2006)
  • Author(s): Takagi, Y
  • Organizer: The 6th International Symposium, "Symposium on Development of Fisheries Science in Asia", 21st Century COE Program, Marine Bio-Manipulation, Frontier for Food Production
  • Place of Presentation: Shanghai Fisheries University, Shanghai, People's Republic of China
  • Year and Date: 2006-12-18
• [Presentation] Potential application of fish scale collagen in tissue engineering (2006)
  • Author(s): Takagi Y, Tohse H, Ura K
  • Organizer: 7th Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
  • Place of Presentation: National Fisheries Research and Development Institute, Busan, Korea
  • Year and Date: 2006-10-20
• [Presentation] Molecular tools to study scale forming cell differentiation -cDNA cloning and expression patterns of BMP2, Runx2 and SPARC- (2006)
  • Author(s): Iimura K, Tohse H, Ura K, Takagi Y
  • Organizer: 7th Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
  • Place of Presentation: National Fisheries Research and Development Institute, Busan, Korea
  • Year and Date: 2006-10-20
• [Presentation] (2006)Discovery of matrix substance involved in hydroxyapatite formation from regenerating fish scales (2006)
  • Author(s): Ogawa, N., H. Tohse, K. Ura, M. Shimizu and Y. Takagi
  • Organizer: Gordon Research Conference on Biomineralization
  • Place of Presentation: Colby-Sawyer College, New London,NH, USA.(Poster presentation)
• [Presentation] (2006)Potential application of fish scale collagen in tissue engineering (2006)
  • Author(s): Takagi, Y., H. Tohse and K. Ura
  • Organizer: 7th Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
  • Place of Presentation: National Fisheries Research and Development Institute,Busan, Korea(Poster presentation)
• [Presentation] (2006)Molecular tools to study scale forming cell differentiation -cDNA cloning and expression patterns of BMP2, Runx2 and SPARC- (2006)
  • Author(s): Iimura K., H. Tohse, K. Ura and Y. Takagi
  • Organizer: 7^<th> Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
  • Place of Presentation: National Fisheries Research and Development Institute, Busan, Korea(Poster presentation)