Development of RNA vaccine with fish retrovirus, a substitution of DNA vaccine, for fish viral diseases

• Project/Area Number: 18380110
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: NISHIZAWA Toyohiko Hokkaido University, 大学院・水産科学研究院, 准教授 (10222184)
• Co-Investigator(Kenkyū-buntansha): YOSHIMIZU Mamoru 北海道大学, 大学院・水産科学研究院, 教授 (40122915)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥14,540,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥1,440,000); Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2006: ¥8,300,000 (Direct Cost: ¥8,300,000)
• Keywords: 魚病 / ワクチン / 魚類ウイルス病 / 感染防御 / レトロウイルス / 魚類ウイルス / 伝染性造血器壊死症ウイルス / ウイルス性神経壊死症ウイルス / ウイルス性出血性配意血症ウイルス / ウイルス性出血性敗血症ウイルス / 遺伝子パッキング

Research Abstract

「RNAワクチン」とは、ウイルス感染防御抗原遺伝子をmRNAとしてウイルス粒子にパッキング後供給し、免疫を誘導するものである。本研究では、魚類のレトロウイルスであるSnRVを用い、魚類病原ウイルスである伝染性造血器壊死症ウイルスおよび神経壊死症ウイルスに対するRNAワクチンの開発を試み、in vitroでのRNAワクチン粒子の構築および発現に成功した。また、「mRNA型」ワクチンの発展型である「ds-RNA型」ワクチンを考案し、その感染防御効果をin vivoで確認した。

Research Products

• [Journal Article] A mixed infection in sevenband grouder Epinephelus septemfasci- antus affected with viral nervous necrosis(VNN) (2008)
  • Author(s): Kokawa, Y, Takami, I., Nishizawa, T, Yoshimizu, M.
  • Journal Title: Aquaculture 284 Pages: 41-45
  • Peer Reviewed
• [Journal Article] Detection of specific antibodies against infectious hematopoietic necrosis virus from rainbow trout sera by ELISA using two novirhabdoviruses (2008)
  • Author(s): Kim, W.-S., Mochizuki, M., Nishizawa, T. and Yoshimizu, M.
  • Journal Title: Fish Pathol 43 Pages: 112-116
  • NAID: 10024481030
  • Peer Reviewed
• [Journal Article] Enhanced propagation of fish nodaviruses in BF-2 cells persistently infected with snaked-head retrovirus(SnRV) (2008)
  • Author(s): Nishizawa, T., Kokawa, Y., Wakayama, T., Kinoshita, S. and Yoshimizu, M.
  • Journal Title: Dis. Aquat. Org 79 Pages: 19-25
  • Peer Reviewed
• [Journal Article] A mixed infection in sevenband grouder Epinephelus septemfasciantus affected with viral nervous necrosis(VNN) (2008)
  • Author(s): Kokawa, Y., Takami, I., Nishizawa, T. and Yoshimizu, M.
  • Journal Title: Aquaculture 284 Pages: 41-45
  • Peer Reviewed
• [Journal Article] Enhanced propagation of fish nodaviruses in BF-2 cells persistently infected with snakedhead retrovirus(SnRV) (2008)
  • Author(s): Nishizawa, T., Kokawa, Y., Wakayama, T., Kinoshita, S. and Yoshimizu, M.
  • Journal Title: Dis. Aquat. Org. 79 Pages: 19-25
  • Peer Reviewed
• [Journal Article] Enhanced propagation of fish nodaviruses in BF-2 cells persistently infected with snakedhead retrovirus (SnRV) (2008)
  • Author(s): Nishizawa, T.
  • Journal Title: Diseases of Aquatic Organisms 79 Pages: 19-25
  • Peer Reviewed
• [Journal Article] Detection of specific antibodies against infectious hematopoietic necrosis virus from rainbow trout sera by ELISA using two novirhabdoviruses (2008)
  • Author(s): Kim, W. S.
  • Journal Title: Fish Pathology 43 Pages: 112-116
  • Peer Reviewed
• [Journal Article] A mixed infection in sevenband grouder Epinephelus septemfasciantus affected with viral nervous necrosis (VNN) (2008)
  • Author(s): Kokawa, Y.
  • Journal Title: Aquaculture 248 Pages: 41-45
  • Peer Reviewed
• [Journal Article] Non-specific adsorption of fish immunoglobulin M (IgM) to blocking reagents on ELISA plate wells (2007)
  • Author(s): Kim, W.-S., Nishizawa, T. and Yoshimizu, M.
  • Journal Title: Dis. Aquat. Org 78 Pages: 55-59
  • Peer Reviewed
• [Journal Article] Genotyping of Korean isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene (2007)
  • Author(s): Kim, W.-S., Nishizawa, T., Oh, M.-J. Park, J.-W., Kurath, G. and Yoshimizu, M.
  • Journal Title: Arch. Virol 152 Pages: 2119-2124
  • Peer Reviewed
• [Journal Article] トラフグ口白症に対する感染防御誘導の試み (2007)
  • Author(s): 高見生雄・西澤豊彦・吉水 守
  • Journal Title: 魚病研究 42 Pages: 67-69
  • NAID: 10018909389
  • Peer Reviewed
• [Journal Article] 口白症トラフグ脳磨砕濾液を用いた人為感染試験によるブリでの発症 (2007)
  • Author(s): 高見生雄・粉川愉記・西澤豊彦・吉水 守
  • Journal Title: 魚病研究 42 Pages: 35-39
  • NAID: 10018909324
  • Peer Reviewed
• [Journal Article] 口白症感染耐過トラフグ血清を用いた口白症関連タンパク質の検出 (2007)
  • Author(s): 高見生雄・粉川愉記・西澤豊彦・吉水 守
  • Journal Title: 魚病研究 42 Pages: 29-34
  • NAID: 10018909311
  • Peer Reviewed
• [Journal Article] Genotyping of Korean isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene (2007)
  • Author(s): Kim, W.-S., Nishizawa, T., Oh, M.-J. Park, J.-W., Kurath, G. and Yoshimizu, M.
  • Journal Title: Arch. Virol. 152 Pages: 2119-2124
  • Peer Reviewed
• [Journal Article] Genotyping of Korean isolates of infectious hematopoietic necrosis virus(IHNV)based on the glycoprotein gene (2007)
  • Author(s): Kim, W.-S.
  • Journal Title: Archives of Virology 152 Pages: 2119-2124
  • Peer Reviewed
• [Journal Article] Non specific reaction of fish antibodies with blocking reagents in antibody detection ELISA system (2007)
  • Author(s): Kim, W.-S.
  • Journal Title: Diseases of Aquatic Organisms 87 Pages: 55-59
  • Peer Reviewed
• [Journal Article] 口白症感染耐過トラフグ血清を用いた口白症関連タンパク質の検出 (2007)
  • Author(s): 高見生雄
  • Journal Title: 魚病研究 42 Pages: 29-34
  • Peer Reviewed
• [Journal Article] 口白症トラフグ脳磨砕濾液を用いた人為感染試験によるブリでの発症 (2007)
  • Author(s): 高見生雄
  • Journal Title: 魚病研究 42 Pages: 35-39
  • Peer Reviewed
• [Journal Article] トラフグ口白症に対する感染防御誘導の試み (2007)
  • Author(s): 高見生雄
  • Journal Title: 魚病研究 42 Pages: 67-69
  • Peer Reviewed
• [Journal Article] Nucleotide diversity of Japanese isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene (2006)
  • Author(s): Nishizawa, T., Kinoshita, S., Higashi, S., Kim, W.-S. and Yoshimizu, M.
  • Journal Title: Dis. Aquat. Org 71 Pages: 267-272
  • Peer Reviewed
• [Journal Article] Genotyping and pathogenicity of viral hemorrhagic septicemia virus (VHSV) from free-living turbot Psetta maxima in Turkish coastal area of the Black Sea (2006)
  • Author(s): Nishizawa, T., Savas, H., Isdan, H., Ustundag, C., Iwamoto, H. and Yoshimizu, M.
  • Journal Title: Appl. Environ. Microbiol 72 Pages: 2373-2378
  • Peer Reviewed
• [Journal Article] A new genotype of lymphocystivirus, LCDV-RF, from lymphocystis diseased rock- fish (2006)
  • Author(s): Kitamura, S.-I., Jung, S.-J., Kim, W.-S., Nishizawa, T., Yoshimizu, M. and Oh, M.-J.
  • Journal Title: Arch. Virol 151 Pages: 607-615
  • Peer Reviewed
• [Journal Article] Genotyping and pathogenicity of viral hemorrhagic septicemia virus (VHSV) from free-living turbot Psetta maxima in Turkish coastal area of the Black Sea (2006)
  • Author(s): Nishizawa, T., Savas, H., Isidan, H., Ustundag, C., Iwamoto, H. and Yoshimizu, M.
  • Journal Title: Appl. Environ. Microbiol 72 Pages: 2373-2378
  • Peer Reviewed
• [Journal Article] Nucleotide diversity of Japanese isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene (2006)
  • Author(s): Nishizawa, T., Kinoshita, S., Higashi, S., Kim, W.-S. and Yoshimizu, M.
  • Journal Title: Dis. Aquat. Org. 71 Pages: 267-272
  • Peer Reviewed
• [Journal Article] A new genotype of lymphocystivirus, LCDV-RF, from lymphocystis diseased rockfish (2006)
  • Author(s): Kitamura, S.-I.
  • Journal Title: Archives of virology 151 Pages: 607-615
• [Journal Article] Genotyping and pathogenicity of viral hemorrhagic septicemia virus (VHSV) from free-living turbot Psetta maxima in Turkish coastal area of the Black Sea (2006)
  • Author(s): Nishizawa, T.
  • Journal Title: Applied and Environmental Microbiology 72 Pages: 2373-2378
• [Journal Article] Nucleotide diversity of Japanese isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene (2006)
  • Author(s): Nishizawa, T.
  • Journal Title: Diseases of Aquatic Organisms 71 Pages: 267-272
• [Presentation] Poly (I:C) 免疫法を用いたマハタのノダウイルスに対する特異免疫の誘導. (2009)
  • Author(s): 粉川愉記・西澤豊彦・高見生雄・吉水 守
  • Organizer: 日本水産学会
  • Place of Presentation: 東京海洋大学, 東京
• [Presentation] Modified ELISA system for detection of specific antibodies againstred seabream iridovirus (RSIV) in yellowtail Seriola quinqueradiata (2008)
  • Author(s): Kwon, S. R.
  • Organizer: The 5th World Fisheries Congress
  • Place of Presentation: Yokohama
  • Year and Date: 2008-10-24
• [Presentation] A live-vaccine of infectious hematopoietic necrosis virus (IHNV) in rainbow trout Oncorhynchus mykiss (2008)
  • Author(s): Kim, H. J.
  • Organizer: The 5th World Fisheries Congress
  • Place of Presentation: Yokohama
  • Year and Date: 2008-10-23
• [Presentation] mixed infection in sevenband grouper Epinephelus septemfasciatus affected with viral nervous necrosis (VNN) (2008)
  • Author(s): Kokawa, Y.
  • Organizer: The 5th World Fisheries Congress
  • Place of Presentation: Yokohama
  • Year and Date: 2008-10-23
• [Presentation] Molecular Epidemiology of fish RNA viruses in the Far Eastern Asia (2008)
  • Author(s): Nishizawa, T.
  • Organizer: The 5th World Fisheries Congress
  • Place of Presentation: Yokohama
  • Year and Date: 2008-10-22
• [Presentation] Productivity of red seabream iridovirus (RSIV) in grouper fin (GF-2) cell line persistently infected with RSIV (2008)
  • Author(s): Kwon, S. R.
  • Organizer: World Aquaculture 2008
  • Place of Presentation: Busan, Korea
  • Year and Date: 2008-05-23
• [Presentation] Molecular Epizootiology of fish diseases by RNA viruses in the Far Eesten Asia (2008)
  • Author(s): Nishizawa, T.
  • Organizer: World Aquaculture 2008
  • Place of Presentation: Busan, Korea
  • Year and Date: 2008-05-23
• [Presentation] Productivity of red seabream iridovirus (RSIV) in grouper fin (GF-2) cell line persistently infected with RSIV (2008)
  • Author(s): Kwon, S.-R., Nishizawa, T., Oh, M.-J. and Yoshimizu, M.
  • Organizer: World Aquaculture
  • Place of Presentation: Busan, Korea.
• [Presentation] Genotyping of Korean isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene (2008)
  • Author(s): Yoshimizu, M., Nishizawa, T., Kim, W.-S. and Oh, M.-J.
  • Organizer: In "the 8th Japan-Korea, Korea-Japan Joint Sympo on Aquaculture 2008"
  • Place of Presentation: Wakayam.
• [Presentation] Molecular epidemiology of fish RNA viruses in the Far Eastern Asia (2008)
  • Author(s): Nishizawa, T. and Yoshimizu, M.
  • Organizer: In the 5th World Fisheries Congress
  • Place of Presentation: Yokohama
• [Presentation] A live-vaccine of infectious hematopoietic necrosis virus (IHNV) in rainbow trout Oncorhynchus mykiss (2008)
  • Author(s): Kim, H.J., Oseko, N., Nishizawa, T. and Yoshimizu, M.
  • Organizer: In the 5th World Fisheries Congress
  • Place of Presentation: Yokohama
• [Presentation] Modified ELISA system for detection of specific antibodies against red seabream iridovirus (RSIV) in yellowtail Seriola quinqueradiata (2008)
  • Author(s): Kwon, S.-R, Takano, R., Nishizawa, T. and Yoshimizu, M.
  • Organizer: In the 5th World Fisheries Congress
  • Place of Presentation: Yokohama
• [Presentation] A mixed infection in sevenband grouper Epinephelus septem- fasciatus affected with viral nervous necrosis (VNN) (2008)
  • Author(s): Kokawa, Y., Takami, I., Nishizawa, T. and Yoshimizu, M.
  • Organizer: In the 5th World Fisheries Congress
  • Place of Presentation: Yokohama
• [Presentation] IHNV株の静岡県産ニジマスに対する病原性について (2008)
  • Author(s): 望月満美子・笠井久会・西澤豊彦・吉水守
  • Organizer: 日本水産学会
  • Place of Presentation: 東海大学, 清水
• [Presentation] Molecular Epidemiology of fish RNA viruses in the Far Eastern Asia (2008)
  • Author(s): Nishizawa, T. and Yoshimizu, M.
  • Organizer: The 5^<th> World Fisheries Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] A live-vaccine of infectious hematopoietic necrosis virus (IHNV) in rainbow trout Oncorhynchus mykiss (2008)
  • Author(s): Kim, H.J. and 3 others
  • Organizer: The 5^<th> World Fisheries Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] A mixed infection in sevenband grouper Epinephelus septemfasciats affected with viral nervous necrosis (VNN) (2008)
  • Author(s): Kokawa, Y. and 3 others
  • Organizer: The 5^<th> World Fisheries Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] Infectious hematopoietic necrosis virus(IHNV, 魚類ラブドウイルス)のアジアにおける分子疫学 (2007)
  • Author(s): 西澤豊彦・吉水 守
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2007-10-23
• [Presentation] Snakehead retrovirus(SnRV)による魚類ノダウイルスの増殖の亢進 (2007)
  • Author(s): 粉川愉記・西澤豊彦・他3名
  • Organizer: 日本魚病学会
  • Place of Presentation: 北海道大学大学院水産科学研究院
  • Year and Date: 2007-09-29
• [Presentation] VNN罹病マハタに存在するノダウイルス以外の濾過性病原体 (2007)
  • Author(s): 粉川愉記・西澤豊彦・他2名
  • Organizer: 日本魚病学会
  • Place of Presentation: 北海道大学大学院水産科学研究院
  • Year and Date: 2007-09-29
• [Presentation] サケ科魚類の抗体検査法について-IHNを例に- (2007)
  • Author(s): 金 尉植・西澤豊彦・吉水 守
  • Organizer: 第1回サケ学研究会
  • Place of Presentation: 北海道大学, 函館
  • Year and Date: 2007-09-24
• [Presentation] Molecular Epozootiology of fish diseases by RNA viruses in the Far Eastern Asia (2007)
  • Author(s): Nishizawa T. and Yoshimizu M.
  • Organizer: The 7th International Symposium on Viruses of Lower Vertebrates
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2007-04-24
• [Presentation] Infectious hematopoietic necrosis virus(IHNV, 魚類ラブドウイルス)のアジアにおける分子疫学 (2007)
  • Author(s): 西澤豊彦・吉水 守
  • Organizer: ウイルス学会
  • Place of Presentation: 札幌
• [Presentation] ニジマス血清中の抗IHNV特異抗体検出ELISAの改良 (2007)
  • Author(s): 金 尉植・西澤豊彦・吉水 守
  • Organizer: 魚病学会
  • Place of Presentation: 北海道大学, 函館
• [Presentation] Snakehead retrovirus (SnRV) による魚類ノダウイルスの増殖の亢進 (2007)
  • Author(s): 粉川愉記・木下真一・若山貴子・西澤豊彦・吉水 守
  • Organizer: 魚病学会
  • Place of Presentation: 北海道大学, 函館
• [Presentation] VNN罹病マハタに存在するノダウイルス以外の濾過性病原体 (2007)
  • Author(s): 粉川愉記・高見生雄・西澤豊彦・吉水 守
  • Organizer: 魚病学会
  • Place of Presentation: 北海道大学, 函館
• [Presentation] ウイルス混合感染の重要性と対策. H19年度日本魚病学会シンポジウム (2007)
  • Author(s): 西澤豊彦・吉水 守
  • Organizer: 魚病学会
  • Place of Presentation: 北海道大学, 函館
• [Presentation] Molecular Epozootiology of fish diseases by RNA viruses in the Far Eastern AsiaIn the 7th Int. Symp. On Viruses of Lower Verte- brates (2007)
  • Author(s): Nishizawa, T and Yoshimizu, M
  • Organizer: Aip
  • Place of Presentation: Oslo, Norway
• [Presentation] Gタンパク質遺伝子に基づく伝染性造血器壊死症ウイルス (IHNV) 韓国株のゲノタイピング (2007)
  • Author(s): 金 尉植・呉 明柱・J.W. Park・G. Kurath・西澤豊彦・吉水 守
  • Organizer: 日本水産学会
  • Place of Presentation: 東京海洋大学
• [Presentation] Molecular Epozootiology of fish diseases by RNA viruses in the Far Eastern Asia (2007)
  • Author(s): Nishizawa, T. and Yoshimizu, M.
  • Organizer: The 7^<th> International Symposium on Viruses of Lower Vertebrates
  • Place of Presentation: Oslo, Norway
• [Presentation] Kuchijir-sho associ- ated proteins (KAPs) in brain tissues of Tiger puffer and Yellowtail. In the 1st Int. Symp (2006)
  • Author(s): Kokawa, Y., Takami, I., Nishizawa, T. and Yoshimizu, M.
  • Organizer: on Viral Nervous Necrosis of Fish
  • Place of Presentation: Hiroshima
• [Presentation] 口白症トラフグの脳組織磨砕濾液を用いたホルマリン不活化ワクチンの有効性. (2006)
  • Author(s): 高見正雄・西澤豊彦・吉水 守
  • Organizer: 日本魚病学会
  • Place of Presentation: 長崎大学
• [Presentation] 感染耐過トラフグ血清を用いた口白症原因体関連タンパク質の検出 (2006)
  • Author(s): 高見正雄・粉川愉記・西澤豊彦・吉水 守
  • Organizer: 日本魚病学会
  • Place of Presentation: 長崎大学
• [Presentation] 口白症トラフグ脳磨砕濾液を用いた人為感染試験によるブリでの発症 (2006)
  • Author(s): 高見正雄・粉川愉記・西澤豊彦・吉水 守
  • Organizer: 日本魚病学会
  • Place of Presentation: 長崎大学
• [Presentation] 抗体検出ELISAにおけるブロッキング剤と魚類IgMの非特異反応について (2006)
  • Author(s): 金 尉植・西澤豊彦・吉水 守
  • Organizer: 日本魚病学会
  • Place of Presentation: 長崎大学
• [Presentation] Evaluation of methods for sero-epidemiology and surveillance of infectous hematopoietic necrosis (2006)
  • Author(s): Yoshimizu, M., Kim, W.-S., kasai, H. and Nishizawa, T.
  • Organizer: In the 11th Int. Symp. on Veterinary Epidemiology and Economics
  • Place of Presentation: Cairns, Australia