Elucidation of the activation network constructed by isotypic complement components diversified in the bony fish

• Project/Area Number: 18380117
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Kyushu University
• Principal Investigator: NAKAO Miki Kyushu University, 大学院・農学研究院, 教授 (50212080)
• Co-Investigator(Kenkyū-buntansha): SOMAMOTO Tomonori 九州大学, 大学院・農学研究院, 准教授 (40403993) ; KATO-UNOKI Yoko 九州大学, 農学部, 技術専門職員 (10380560) ; 無津呂 淳一 福岡女子短期大学, 食物栄養科学科・講師, 講師 (40399100)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,090,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥2,490,000); Fiscal Year 2008: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2007: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2006: ¥7,300,000 (Direct Cost: ¥7,300,000)
• Keywords: 免疫 / 補体 / 硬骨魚類 / 多様性 / アイソタイプ / 遺伝子 / 発現 / 増養殖 / 魚類 / 多重化 / 機能分化 / 自然免疫 / 結合特異性 / コイ / 組換えタンパク / 抗体

Research Abstract

コイを実験魚として用い、魚類において多様化した補体成分アイソタイプの機能的多様性を解析し、補体アイソタイプ間、補体アイソタイプと異物感の反応ネットワークを解明することを目的とした。補体成分C1の亜成分アイソタイプをクローニングおよび精製するとともに、組換え体あるいは天然のC3、C4、C7およびMBLアイソタイプを調製し、それらの相互作用の多様性を明らかにした。特に、C3の反応については魚類特異的アイソタイプの多機能性が明らかとなった。

Research Products

• [Journal Article] In vitro generation of viral-antigen dependent cytotoxic T-cells from ginbuna crucian carp (2009)
  • Author(s): Somamoto T, Okamoto N, Nakanishi T, Ototake M, and Nakao M
  • Journal Title: Carassius auratus langsdorfii, Virology (in press)
• [Journal Article] Extensive Expansion and Diversification of the Chemokine Gene Family in Zebrafish : Identification of a Novel Chemokine Subfamily CX (2008)
  • Author(s): Nomiyama H, Hieshima K, Osada N, Kato-Unoki Y, Otsuka-Ono K, Takegawa S, Izawa T, Yoshizawa A, Kikuchi Y, Tanase S, Miura R, Kusada J, Nakao M, Yoshie O
  • Journal Title: BMC Genomics 9 Pages: 222-222
• [Journal Article] Molecular cloning of CD4 from ginbuna crucian carp Carassius auratus langsdorfii (2008)
  • Author(s): Nonaka S, Somamoto T, Kato-Unoki Y, Ototake M, Nakanishi T, Nakao M
  • Journal Title: Fisheries Science 74(2) Pages: 341-346
  • NAID: 10020478135
• [Journal Article] Extensive expansion and diversification of the chemokine gene family in zebrafish : identification of a novel chemokine subfamily CX. (2008)
  • Author(s): Nomiyama H, et al.
  • Journal Title: Bio Med Central Genomics 9 Pages: 222-222
  • Peer Reviewed
• [Journal Article] Molecular cloning of CD4 from ginbuna crucian carp Carassius auratus langsdorfii. (2008)
  • Author(s): Nonaka S, et al.
  • Journal Title: Fisheries Science 74 Pages: 341-346
  • Peer Reviewed
• [Journal Article] Molecular cloning of CD4 from gihbuna crucian carp,Carassius auratus langsdorfii. (2008)
  • Author(s): Nonaka S, SomamotoT, et. al.
  • Journal Title: Fish Science 74 Pages: 341-346
  • Peer Reviewed
• [Journal Article] Molecular cloning, structural analysis and expression of complement component Bf/C2 genes in the nurse shark, Ginglymostoma cirratum (2007)
  • Author(s): Shin DH, Webb B, Nakao M, Smith SL
  • Journal Title: Dev Comp Immunol 31(11) Pages: 1168-82
• [Journal Article] 補体活性化の系統発生 (2007)
  • Author(s): 中尾実樹、杣本智軌
  • Journal Title: 臨床免疫・アレルギー科 48(1) Pages: 80-85
  • NAID: 40015604924
• [Journal Article] Molecular cloing,structural analysis and expression of complement component Bf/C2 genes in the nurse shark,Ginglymostoma cirratum (2007)
  • Author(s): Shin DH, Webb B, Naliao M, Smith SL
  • Journal Title: Developmental & Comparative Immunology 31 Pages: 1168-1182
  • Peer Reviewed
• [Journal Article] 補体活性化の系統発生 (2007)
  • Author(s): 中尾 実樹, 杣本 智軌
  • Journal Title: 臨床免疫・アレルギー科 48 Pages: 80-85
  • NAID: 40015604924
• [Journal Article] Molecular cloning of cDNA encoding C3d, an activated fragment of the complement component C3, from bony fish relevant to aquaculture (2007)
  • Author(s): Yoshida T, Kato-Unoki Y, Somamoto T, Kondo M, Yamashita H, Peralta JP, Hidalgo ARD, Nakao M
  • Journal Title: UPV Journal of Natural Sciences 11 (In press)
• [Journal Article] Lectin pathway of bony fish complement : identification of two homologs of the mannose-binding lectin associated with MASP2 in the common carp (Cyprinus carpio) (2006)
  • Author(s): Nakao M, Kajiya T, Sato Y, Somamoto T, Kato-Unoki Y, Matsushita M, Nakata M, Fujita T, Yano T
  • Journal Title: J Immunol 177(8) Pages: 5471-9
• [Journal Article] Expression profiles of TCRbeta and CD8alpha mRNA correlate with virus-specific cell-mediated cytotoxic activity in ginbuna crucian carp (2006)
  • Author(s): Somamoto T, Yoshiura Y, Sato A, Nakao M, Nakanishi T, Okamoto N, Ototake M
  • Journal Title: Virology 348(2) Pages: 370-7
• [Journal Article] Lectin pathway of bony fish complement : identification of two homologs of the mannose-binding lection associated with MASP2 in the common carp (Cyprinus carpio) (2006)
  • Author(s): Nakao M, Kajiya T, Sato Y, Somamoto T, Kato-Unoki Y, Matsushita M, Nakata M, Fujita T, Yano T
  • Journal Title: Journal of Immunology 177 Pages: 5471-5479
• [Journal Article] Diversified components of the bony fish complement system : more genes for robuster innate defense? (2006)
  • Author(s): Nakao M, Kato-Unoki Y, Nakahara M, Mutsuro J, Somamoto T.
  • Journal Title: Adv Exp Med Biol. 586 Pages: 121-138
• [Journal Article] Characterization of shark complement factor I gene(s) : Genomic analysis of a novel shark-specific sequence
  • Author(s): Shin DH, Webb BM, Nakao M, Smith SL
  • Journal Title: Molecular Immunology doi : 10.1016/j.molimm.2009.04.002,2009.05
• [Presentation] The Complement System of Teleost : Isotypic Diversity of Its Components in the Structure (2008)
  • Author(s): Nakao M, Somamoto T, Kato-Unoki Y, and Mutsuro J
  • Organizer: Expression, and Functions World Fisheries Congress 2008
  • Year and Date: 2008-10-21
• [Presentation] Identification and Expression Analysis of Two Isotypes of Carp Complement Component C7 (2008)
  • Author(s): Akahoshi S, Somamoto T, and Nakao M
  • Organizer: World Fisheries Congress 2008
  • Year and Date: 2008-10-21
• [Presentation] Functional Analysis of Carp Complement C3 Isotypes Using Monoclonal Antibodies (2008)
  • Author(s): Ichiki S, Kato-Unoki Y, Somamoto T, and Nakao M
  • Organizer: World Fisheries Congress 2008
  • Year and Date: 2008-10-21
• [Presentation] Molecular identification of a novel regulator of complement activation in teleost immune system (2008)
  • Author(s): Tsujikura M, Somamoto T, Kato-Unoki Y, and Nakao M
  • Organizer: World Fisheries Congress 2008
  • Year and Date: 2008-10-21
• [Presentation] The Complement System of Teleost : Isotypic Diversity of Its Components in the Structure, Expression, and Functions (2008)
  • Author(s): Nakao M, et al.
  • Organizer: World Fisheries Congress
  • Place of Presentation: 横浜市
  • Year and Date: 2008-10-21
• [Presentation] Identification and Expression Analysis of Two Isotypes of Carp Complement Component C7 (2008)
  • Author(s): Akahoshi S, Somamoto T, Nakao, M
  • Organizer: World Fisheries Congress
  • Place of Presentation: 横浜市
  • Year and Date: 2008-10-21
• [Presentation] Functional Analysis of Carp Complement C3 Isotypes Using Monoclonal Antibodies (2008)
  • Author(s): Ichiki S, Kato-Unoki Y, Somamoto T, Nakao M.
  • Organizer: World Fisheries Congress
  • Place of Presentation: 横浜市
  • Year and Date: 2008-10-21
• [Presentation] Molecular Identification of a Novel Regulator of Complement Activation in Teleost Immune System (2008)
  • Author(s): Tsujikura M, Somamoto T, Kato-Unoki Y, Nakao M.
  • Organizer: World Fisheries Congress
  • Place of Presentation: 横浜市
  • Year and Date: 2008-10-21
• [Presentation] Functional divergence and its biological significance of complement component isotypes in bony fish. (2008)
  • Author(s): Nakao M
  • Organizer: Swedish-Japan STINT-meeting on Innate Immunity
  • Year and Date: 2008-10-17
• [Presentation] Functional divergence and its biological significance of complement component isotypes in bony fish (2008)
  • Author(s): Nakao M.
  • Organizer: Swedish-Japan STINT-meeting on Innate Immunity
  • Place of Presentation: 福岡市
  • Year and Date: 2008-10-17
• [Presentation] ギンブナ由来細胞株における4種のMHCクラスI遺伝子のクローニングおよび、CHNV感染後の発現動態 (2008)
  • Author(s): 占部慎二、鮫島史朗、杣本智軌、中西照幸、中尾実樹
  • Organizer: 日本比較免疫学会
  • Year and Date: 2008-08-25
• [Presentation] コイおよびゼブラフィッシュの膜型補体制御因子 (2008)
  • Author(s): 辻倉正和・杣本智軌・鵜木陽子・中尾実樹
  • Organizer: 日本比較免疫学会
  • Year and Date: 2008-08-25
• [Presentation] 補体レクチン経路に関与するコイ血清レクチンの多様性 (2008)
  • Author(s): 一木昭土、畑中大作、杣本智軌、中尾実樹
  • Organizer: 日本比較免疫学会
  • Year and Date: 2008-08-25
• [Presentation] ギンブナ由来細胞株における4種のMHCクラスI遺伝子のクローニングおよび, CHNV感染後の発現動態 (2008)
  • Author(s): 占部慎二, 鮫島史朗, 杣本智軌, 中西照幸, 中尾実樹
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2008-08-25
• [Presentation] コイおよびゼブラフィッシュの膜型補体制御因子 (2008)
  • Author(s): 辻倉正和, 杣本智軌, 鵜木陽子, 中尾実樹
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2008-08-25
• [Presentation] 補体レクチン経路に関与するコイ血清レクチンの多様性 (2008)
  • Author(s): 一木昭土, 畑中大作, 杣本智軌, 中尾実樹
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2008-08-25
• [Presentation] C3 and C4 isotypes with a non-catalyzed thioester in carp fish : phylogenetic implications (2008)
  • Author(s): Nakao M, Mutsuro J, Kato-Unoki Y, Yano T, and Dodds AW
  • Organizer: Immunochemistry in Oxford Symposium
  • Year and Date: 2008-07-20
• [Presentation] C3 and C4 isotypes with a non-catalyzed thioester in carp fish : phylogenetic implications (2008)
  • Author(s): Nakao M, et al.
  • Organizer: Immunochemistry in Oxford Symposium
  • Place of Presentation: Univ. of Oxford, Oxford, England
  • Year and Date: 2008-07-20
• [Presentation] コイ補体成分C7アイソタイプのクローニングと発現解析 (2008)
  • Author(s): 赤星佐和、辻倉正和、杣本智軌、中尾実樹
  • Organizer: 補体シンポジウム
  • Year and Date: 2008-07-11
• [Presentation] コイ補体成分C7アイソタイプのクローニングと発現解析 (2008)
  • Author(s): 赤星佐和, 辻倉正和, 杣本智軌, 中尾実樹
  • Organizer: 第45回補体シンポジウム
  • Place of Presentation: 北海道大学医学部
  • Year and Date: 2008-07-11
• [Presentation] Structural and functional diversity of the complement components in bony fish : implication for innate immune defense. (2008)
  • Author(s): Nakao M, Mutsuro J, Kato-Unoki Y, and Somamoto T
  • Organizer: International Conference of Advanced Research on Marine Bioresources
  • Year and Date: 2008-05-08
• [Presentation] Structural and functional diversity of the complement components in bony fish : implication for innate immune defence (2008)
  • Author(s): Nakao M, et al.
  • Organizer: International Conference of Advanced Research on Marine Bioresources
  • Place of Presentation: National Taiwan Ocean Univ. Keelun, Taiwan
  • Year and Date: 2008-05-08
• [Presentation] ウイルス特異的障害活性を有するギンブナ培養リンパ球のCD8αとTCRSSのmRNAの発現解析 (2008)
  • Author(s): 杣本智軌・中西照幸・乙竹充・中尾実樹
  • Organizer: 日本水産学会
• [Presentation] 魚類における新規補体制御因子の同定 (2008)
  • Author(s): 辻倉正和・杣本智軌・鵜木陽子・中尾実樹
  • Organizer: 日本水産学会
• [Presentation] Structural and functional diversity of the complement components in bony fish : implication for innate immune defense (2008)
  • Author(s): Nakao M, Mutsuro J, Kato-Unoki Y, and Somamoto T
  • Organizer: International Conference of Advanced Research on Marine Bioresources
• [Presentation] コイ補体B因子アイソタイプ遺伝子の構造と発現制御 (2008)
  • Author(s): 岩谷健太郎・杣本智軌・鵜木陽子・中尾実樹
  • Organizer: 日本水産学会
• [Presentation] 2種類のギンブナCD4様分子 (2008)
  • Author(s): 野中誠子・杣本智軌・中西照幸・乙竹充・中尾実樹
  • Organizer: 日本水産学会九州支部会
• [Presentation] Identification and expression analysis of a novel interleukine 8 (IL-8)-like CXC chemokine in carp (Cyprinus carpio) (2007)
  • Author(s): Nevien K. Abdelkhalek, Komiya A, Kato-Unoki Y, Somamoto T, Nakao M
  • Organizer: 日本魚病学会
• [Presentation] Functional analysis of carp C3 isotypes using monoclonal antibodies (2007)
  • Author(s): Ichiki S, Kato-Unoki Y, Somamoto T, and Nakao M
  • Organizer: Japan-Germany International Cooperative Project on Education and Research
• [Presentation] Antibacterial substances from bony fish : structure, function, and application (2007)
  • Author(s): Nakao M
  • Organizer: Philippines Society of Microbiology
• [Presentation] コイ補体成分C1タンパク質の同定 (2007)
  • Author(s): 市居敬、杣本智軌、鵜木(加藤)陽子、中尾実樹
  • Organizer: 補体シンポジウム
• [Presentation] モノクローナル抗体を用いたコイC3アイソタイプの機能解析 (2007)
  • Author(s): 一木智子、鵜木(加藤)陽子、杣本智軌、中尾実樹
  • Organizer: 補体シンポジウム
• [Presentation] 魚類における新規補体制御因子の同定 (2007)
  • Author(s): 辻倉正和、杣本智軌、鵜木(加藤)陽子、中尾実樹
  • Organizer: 補体シンポジウム
• [Presentation] ギンブナ頭腎、体腎、脾臓のExpressedSequence Tag解析 (2007)
  • Author(s): 鮫島史朗、鵜木陽子、杣本智軌、中尾実樹
  • Organizer: 日本比較免疫学会
• [Presentation] 1, クローンギンブナ由来細胞株におけるMHCクラスIによる抗原提示機構関連遺伝子のクローニング (2007)
  • Author(s): 杣本智軌、占部慎二、鮫島史朗、中西照幸、中尾実樹
  • Organizer: 日本比較免疫学会
• [Presentation] コイの個体発生における補体成分アイソタイプの発現解析 (2007)
  • Author(s): 大蔵千恵、杣本智軌, 近藤昌和, 中尾実樹
  • Organizer: 日本比較免疫学会
• [Presentation] ゼブラフィッシュ補体成分の遺伝子の同定と発現解析 (2007)
  • Author(s): 新原美樹、杣本智軌、Vo Kha Tam、加藤陽子、中尾実樹
  • Organizer: 日本水産学会大会
• [Presentation] コイ補体成分C4, C5の組換え体の発現と機能解析 (2007)
  • Author(s): 中尾実樹、加藤陽子、市居敬、杣本智軌
  • Organizer: 日本水産学会大会
• [Presentation] 発現パターンから見た魚類補体の生体防御機能の推定 (2007)
  • Author(s): 大蔵千恵、新原美樹、杣本智軌、中尾実樹、近藤昌和
  • Organizer: 南中九州・西四国水族防疫会議
• [Presentation] コイ補体成分C4,C5の組換え体の発現と機能解析 (2007)
  • Author(s): 中尾 実樹・加藤 陽子・市居 敬・杣本 智軌
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
• [Presentation] ゼブラフィッシュ補体成分の遺伝子の同定と発現解析 (2007)
  • Author(s): 新原 美樹・杣本 智軌・Vo Kha Tam・加藤 陽子・中尾 実樹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
• [Presentation] 魚類における新規補体制御因子の同定 (2007)
  • Author(s): 辻倉 正和, 杣本 智軌、鵜木 (加藤) 陽子, 中尾 実樹
  • Organizer: 補体シンポジウム
  • Place of Presentation: 東海大学工学部
• [Presentation] モノクローナル抗体を用いたコイC3アイソタイプの機能解析 (2007)
  • Author(s): 一木 智子, 鵜木 (加藤) 陽子, 杣本 智軌, 中尾 実樹
  • Organizer: 補体シンポジウム
  • Place of Presentation: 東海大学工学部
• [Presentation] コイ補体成分C1タンパク質の同定 (2007)
  • Author(s): 市居 敬, 杣本 智軌, 鵜木 (加藤) 陽子, 中尾 実樹
  • Organizer: 補体シンポジゥム
  • Place of Presentation: 東海大学工学部
• [Presentation] コイの個体発生における補体成分アイソタイプの発現解析 (2007)
  • Author(s): 大蔵 千恵, 杣本 智軌, 近藤 昌和, 中尾 実樹
  • Organizer: 日本比較免疫学会学術集会
  • Place of Presentation: 東京大学水産実験所
• [Presentation] Antibacterial substances from bony fish: stucture,function,and application (2007)
  • Author(s): Miki Nakao
  • Organizer: Annual Meeting of the Philippine Society of Microbiology
  • Place of Presentation: Aklan, Philippines
• [Presentation] コイの新奇補体制御因子のcDNAクローニング (2006)
  • Author(s): 吉田大志、杣本智軌, 加藤陽子, 中尾実樹
  • Organizer: 日本比較免疫学会学術集会
• [Presentation] ゼブラフィッシュ新奇補体制御因子のc DNAクローニング (2006)
  • Author(s): 辻倉正和・杣本智軌・加藤陽子・中尾実樹
  • Organizer: 日本魚病学会
• [Presentation] Molecular cloning of C3d, a fragment from complement component C3 with possible adjuvant activity, from marine cultured fish (2006)
  • Author(s): Nakao M, Yoshida T, Jose P. Peralta and Aklani Rose D. Hidalgo
  • Organizer: International Forum on the Coastal Environment and Utitlization of Fisheries Resources
• [Presentation] EST Analysis of mRNAs Expressed in Gill and Intestine of Carp (Cyprinus carpio. L) (2006)
  • Author(s): Kato-Unoki Y, Komiya A, Sameshima S, Nakao M
  • Organizer: Asia-Pacific Aquatic Genomics Symposium 2006
• [Presentation] Identification of the genes encoding complement components from the zebrafish genome database (2006)
  • Author(s): Shinbara M, Vo Kha Tam, Kato-Unoki Y, Somamoto T, and Nakao M
  • Organizer: Asia-Pacific Aquatic Genomics Symposium 2006
• [Presentation] Molecular cloning and characterization of CD4 in ginbuna crucian carp (2006)
  • Author(s): Somamoto T, Nonaka S, Kato-Unoki Y, Ototake M, Nakanishi T and Nakao M
  • Organizer: 10th Congress of International Society for Developmental and Comparative Immunology
• [Presentation] Molecular cloning and characterization of the complement C1q A, B, and C chains in common carp (2006)
  • Author(s): Somamoto T, Vo Kha Tam, Kato-Unoki Y and Nakao M
  • Organizer: 10th Congress of International Society for Developmental and Comparative Immunology
• [Presentation] Evolving through annual meetings (2006)
  • Author(s): Nakao M, JADCI
  • Organizer: 10th Congress of International Society for Developmental and Comparative Immunology
• [Presentation] The complement system in invertebrates and lower vertebrates (2006)
  • Author(s): Nakao M
  • Organizer: 10th Congress of International Society for Developmental and Comparative Immunology
• [Presentation] Diversification of complement component isotypes in the common carp : Expression and some functional aspects (2006)
  • Author(s): Okura C, Iwatani K, Shibata D, Kato-Unoki Y, Somamoto T and Nakao M
  • Organizer: 10th Congress of International Society for Developmental and Comparative Immunology
• [Presentation] コイ補体C1q A, B, C鎖のcDNAのクローニング (2006)
  • Author(s): 杣本智軌、Vo Kha Tam、加藤陽子、中尾実樹
  • Organizer: 補体シンポジウム
• [Book] Diversified components of the bony fish complement system : more genes for robuster innate defense? (2006)
  • Author(s): Nakao M, Kato-Unoki Y, Nakahara M, Mutsuro J, Somamoto T
  • Publisher: Adv Exp Med Biol