Development of estimation method for mammalian oocyte quality based on molecular mechanism of follicular selection
Project/Area Number |
18380164
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied animal science
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Research Institution | The University of Tokyo |
Principal Investigator |
MANABE Noboru The University of Tokyo, 大学院・農学生命科学研究科, 教授 (80243070)
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Co-Investigator(Kenkyū-buntansha) |
MIYANO Takashi 神戸大学, 大学院・農学研究科, 教授 (80200195)
OKUDA Kiyoshi 岡山大学, 大学院・自然科学研究科, 教授 (40177168)
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Project Period (FY) |
2006 – 2009
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Project Status |
Completed (Fiscal Year 2009)
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Budget Amount *help |
¥18,000,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2008: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2007: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2006: ¥6,300,000 (Direct Cost: ¥6,300,000)
|
Keywords | 家畜繁殖 / 卵胞選抜 / 卵母細胞 / アポトーシス / 細胞死阻害因子 / 卵子品質 / 卵胞閉鎖 / 顆粒層細胞 / 哺乳類 / 卵巣 / 発現制御 / 卵胞 / 細胞死受容体 / 選択的死滅 / カスパーゼ / アポトーシス阻害因子 |
Research Abstract |
Many primordial follicles are present in the mammalian ovary. Less than 1% develop and ovulate, and the remainders are eliminated via atresia. The endocrinological mechanisms involved in follicular development have been characterized, but the precise mechanisms involved in the regulation of follicule selection remain unknown. Recent studies suggest that apoptosis in granulosa cells plays a crucial role in follicular selection. Notably, death ligand and receptor system and intracellular signaling are the key mechanisms regulating granulosa cell apoptosis. In this study, the mechanisms of granulosa cell apoptosis regulated by death ligand and receptor system and signaling, and the roles of apoptosis inhibitory factors (cellular FLICE-like inhibitory protein : cFLIP and X-linked inhibitor of apoptosis protein : XIAP) were revealed. The inhibitors are useful to assess damaged and/or healthy oocytes.
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Report
(6 results)
Research Products
(186 results)
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[Journal Article] Role of cell death ligand and receptor system on regulation of follicular atresia in pig ovaries2008
Author(s)
Manabe N, Matsuda F, Goto Y, Maeda A, ChengY, Nakagawa S, Inoue N, Wongpanit K, Jin HZ, Gonda H, Li JY
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Journal Title
Reprod Dom Anim 43
Pages: 268-272
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[Journal Article] Role of cell death ligand and receptor system on regulation of follicular atresia in pig ovaries.2008
Author(s)
Noboru Manabe, Fuko Minehata, Yasufumi Goto, Akihisa Maeda, Yuan Cheng, Shoma Nakagawa, Naoko Inoue, Kannika Wongpanit, Hua-Zi Jin, Hiroshi Gonda, Jun-You Li
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Journal Title
Reproduction in Domestic Animals 43
Pages: 268-272
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Peer Reviewed
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[Journal Article] Cell death ligand and receptor dependent regulatory mechanisms of follicule selection in porcine ovaries2007
Author(s)
Noboru Manabe, Akihisa Maeda, Yasufumi Goto, Fuko Minehata, Naoko Inoue, Yuan Cheng, Huazi Jin, Kamika Wongpanit, Shoma Nakagawa, Jun-You Li, Takashi Miyano, Kazuhiro Sakamaki
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Journal Title
Morphology 2
Pages: 28-40
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Peer Reviewed
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[Journal Article] The initiator caspase, caspase-10beta, and the BH-3-only molecule, Bid, demonstrate evolutionary conservation in Xenopus of their pro-apoptotic activities in the extrinsic and intrinsic pathways.2006
Author(s)
Katsuya Kominami, Chiyo Takagi, Tomoko Kurata, Atsushi Kitayama, Masami Nozaki, Tatsuya Sawasaki, Keisuke Kuida, Yaeta Endo, Noboru Manabe, Naoto Ueno, Kazuhiro Sakamaki
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Journal Title
Genes to Cells 11・5
Pages: 701-717
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[Journal Article] Changes in expression of anti-apoptotic protein, cFLIP, in granulose cells during follicular atresia in porcine ovaries.2006
Author(s)
Noboru Manabe, Fuko Matsuda-Minehata, Yasufumi Goto, Akihisa Maeda, Yuan Cheng, Takafumi Sai, Miyuki Kohori, Maiko Endo, Junyou Li
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Journal Title
European Journal of Cellular and Molecular Physiopathology 44・1
Pages: 120-124
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[Journal Article] Production of infectious hepatitis C virus particles in three-dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b.2005
Author(s)
Kyoko Murakami, Koji Ishii, Yousuke Ishihara, Sayaka Yoshizaki, Keiko Tanaka, Yasufumi Gotoh, Hideki Aizaki, Michinori Kohara, Hiroshi Yoshioka, Yuichi Mori, Noboru Manabe, Ikuo Shoji, Tetsutaro Sata, Ralf Bartenschlager, Yoshiharu Matsuura, Tatsuo Miyamura, Tetsuro Suzuki.
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Journal Title
Virology 351・3
Pages: 381-392
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[Presentation] Growth initiation of pig oocytes.2009
Author(s)
Takashi Miyano, Muhamad Moniruzzaman, Noboru Manab
Organizer
The 6^<th> Asian Reproductive Biotechnology Conference
Place of Presentation
Siem Reap City, Cambodia
Year and Date
2009-11-17
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[Presentation] Regulation of follicular at resia by eceptor system2007
Author(s)
Noboru Manabe, Fuko Matsuda-Minehata, Yasufumi Goto, Akihisa Maeda, YuanCheng, Hiroshi Gonda, Kannika WongPanit, Huazi Jin, Junyou Li, Kazuhiro Sakamaki, Takashi Miyano
Organizer
The 4th Asian Reproductive Biotechnology Conference
Place of Presentation
Singapore,Singapor
Year and Date
2007-11-25
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[Presentation] Follicle at resia and its regulation:Granulosa cell,a type II apoptotic cell,regulates follicular atresia in porcine ovaries2007
Author(s)
Noboru Manabe, FUko Matsuda-Minehata, Yasufumi Goto, Akihisa Maeda, Yuan Cheng, Fanzu Jin, Junyou Li, Kanika Wongpanit
Organizer
The 2nd Conference on Asian Association of Veterinary Anatomists
Place of Presentation
Bangkon,Thailand
Year and Date
2007-09-13
Related Report
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[Presentation] Localization of anti-apoptosis protein,cflip,in mammalian ovaries2007
Author(s)
Hiroshi Gonda, Kannika Wongpanit, Akihisa Maeda, Yuan Cheng, Junyou Li, Fuko Matsuda-Minehata, Yasufumi Goto, Noboru Manabe
Organizer
The 2nd Conference on Asian Association of Veterinary Anatomists
Place of Presentation
Bangkon,Thailand
Year and Date
2007-09-13
Related Report
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