Study on production domestic animals biosynthesized conjugated fatty acids that carries anti-obesity effects

• Project/Area Number: 18380169
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Kinki University
• Principal Investigator: SAEKI Kazuhiro Kinki University, 生物理工学部, 教授 (10298937)
• Co-Investigator(Kenkyū-buntansha): HOSOI Yoshihiko 近畿大学, 生物理工学部, 教授 (70192739) ; MATSUMOTO Kazuya 近畿大学, 生物理工学部, 教授 (20298938) ; MITANI Tasuku 近畿大学, 先端技術総合研究所, 准教授 (10322265) ; TAGUCHI Yoshitomo 近畿大学, 生物理工学部, 講師 (70309269)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥15,020,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥2,820,000); Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2007: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2006: ¥2,800,000 (Direct Cost: ¥2,800,000)
• Keywords: クローン家畜 / トランスジェニック家畜 / 共役脂肪酸 / ウシ / 筋衛星細胞 / リノール酸異性化酵素遺伝子 / 脂肪細胞 / 共役リノール酸 / 腸内嫌気性細菌 / クローン胚 / 植物のFAD3遺伝子 / n-3系脂肪酸 / DHA

Research Abstract

Conjugated linoleic acid has been reported to be effective for cancers and obesity. In this study, a gene for an isomerase for conjugated linoleic acid (PAISOM) was introduced into bovine cells. We obtained cells stably transfected with PAISOM gene. We also successfully produced cloned embryos from the PAISOM-cells. However, Gas chromatography of fatty acids in the PAISOM-cells did not indicate the conjugated linoleic acid, but we detected a peak unknown but likely to be conjugated arachidonic acid that were elongated from conjugated linoleic acid.

Research Products

• [Journal Article] Comparison of early development in utero of cloned fetuses derived from bovine fetal fibroblasts at the G1 and G0/G1 Phases. (2010)
  • Author(s): Ideta A, Hayama K, Urakawa M, Tsuchiya K, Aoyagi Y, Saeki K
  • Journal Title: Anim Reprod Sci
  • Peer Reviewed
• [Journal Article] Synchronization of cell cycle at late G1 phase for evaluating embryonic development of bovine somatic cell nuclear transfer embryos. (2010)
  • Author(s): Iwamoto D, Ogi M, Tomita S, Kasamatsu A, Tamari T, Ideta A, Urakawa M, Matsumoto K, Hosoi Y, Iritani A, Aoyagi Y, Saeki K
  • Journal Title: Mem School BOST Kinki Univ 24
  • NAID: 120005736384
  • Peer Reviewed
• [Journal Article] Comparison of early development in utero of cloned fetuses derived from bovine fetal fibroblasts at the G1 and G0/G1 Phases (2010)
  • Author(s): Ideta A
  • Journal Title: Anim Reprod Sci (印刷中)
  • Peer Reviewed
• [Journal Article] 黒毛和種種雄牛「安福」号の体細胞クローン「望安福」誕生 (2009)
  • Author(s): 星野洋一郎, 林登, 傍島英雄, 谷口俊仁, 小林直彦, 坂口慎一, 佐伯和弘
  • Journal Title: 日本胚移植学雑誌 31 Pages: 169-175
• [Journal Article] Functional expression of a humanized gene for an omega-3 fatty acid desaturase from scarlet flax in transfected bovine adipocytes and bovine embryos cloned from the cells. (2009)
  • Author(s): Indo Y, Tatemizo A, Abe Y, Suzuki I, Matsumoto K, Hosoi Y, Kinoshita M, Mikami K, Murata N, Iritani A, Saeki K
  • Journal Title: Biochim Biophys Acta 1791 Pages: 183-190
  • Peer Reviewed
• [Journal Article] Resurrection of a bull by cloning from organs frozen without cryoprotectant in a-80 oC freezer for a decade. (2009)
  • Author(s): Hoshino Y, Hayashi N, Taniguchi S, Kobayashi N, Sakai K, Otani T, Iritani A, Saeki K
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Enhancement of histone acetylation by trichostatin A during in vitro fertilization of bovine oocytes affects cell number of the inner cell mass of the resulting blastocysts (2009)
  • Author(s): Ikeda S
  • Journal Title: Zygote 17 Pages: 209-215
  • Peer Reviewed
• [Journal Article] 植物遺伝子によるn-3系多価不飽和脂肪酸を生合成する形質転換家畜の生産 (2009)
  • Author(s): 印藤頼子
  • Journal Title: オレオサイエンス 9 Pages: 375-384
  • Peer Reviewed
• [Journal Article] Functional expression of a humanized gene for an ・-3 fatty acid desaturase from scarlet flax intransfected bovine adipocytes and bovineembryos cloned from the cells (2009)
  • Author(s): Indo Y, Tatemizo A, Abe Y, Suzuki I, Matsumoto K, Hosoi Y, Kinoshita M, Mikami K, Murata N, Iritani A, Saeki K.
  • Journal Title: Biochim Biophys Acta 1791 Pages: 183-190
• [Journal Article] Enhancement of histone acetylation by trichostatin A during in vitro fertilization of bovineoocytes affects cell number of the inner cell mass of the resulting blastocysts (2009)
  • Author(s): Ikeda S, Tatemizo A, Iwamoto D, Taniguchi S, Hoshino Y, Amano T, Matsumoto K, HosoiY, Iritani A, Saeki K
  • Journal Title: Zygote (in press)
• [Journal Article] Functional expression of a humanized gene for an omega-3 fatty acid desaturase from scarlet flax in transfected bovine adipocytes and bovine embryos cloned from the cells. (2009)
  • Author(s): Indo Y
  • Journal Title: Biochim Biophys Acta 1791 Pages: 183-190
  • Peer Reviewed
• [Journal Article] Enhancement of histone acetylation by trichostatin A during in vitro fertilization of bovine oocytes affects cell number of the inner cell mass of the resulting blastocysts. (2009)
  • Author(s): Ikeda S
  • Journal Title: Zygote (印刷中)
  • Peer Reviewed
• [Journal Article] 植物由来脂肪酸不飽和化酵素遺伝子を哺乳動物細胞において高発現させるためのコドン使用頻度の最適化 (2008)
  • Author(s): 印藤頼子, 松本和也, 細井美彦, 鈴木石根, 村田紀夫, 入谷明, 佐伯和弘
  • Journal Title: 近畿大学生物理工学部紀要 22 Pages: 33-41
  • NAID: 120005734926
  • Peer Reviewed
• [Journal Article] Interaction between embryos and culture conditions during in vitro development. (2008)
  • Author(s): Nagao Y, Iijima R, Saeki K
  • Journal Title: Zygote 16 Pages: 127-133
  • Peer Reviewed
• [Journal Article] Interaction between embryos and culture conditions during in vitro development (2008)
  • Author(s): Y Nagao, R Iijima, K Saeki
  • Journal Title: Zygote 16 Pages: 127-133
• [Journal Article] 植物由来脂肪酸不飽和化酵素遺伝子を哺乳動物細胞において高発現させるためのコドン使用頻度の最適化 (2008)
  • Author(s): 印藤頼子, 松本和也, 細井美彦, 鈴木石根, 村田紀夫,入谷明, 佐伯和弘
  • Journal Title: 近畿大学生物理工学部紀要 22 Pages: 33-41
  • NAID: 120005734926
• [Journal Article] Interaction between embryos and culture conditions during in vitro development (2008)
  • Author(s): Y Nagao
  • Journal Title: Zygote 16 Pages: 127-133
  • Peer Reviewed
• [Journal Article] 植物由来脂肪酸不飽和化酵素遺伝子を哺乳動物細胞において高発現させるためのコドン使用頻度の最適化 (2008)
  • Author(s): 印藤頼子
  • Journal Title: 近畿大学生物理工学部紀要 22 Pages: 33-41
  • Peer Reviewed
• [Journal Article] 初期G1期細胞によるウシ核移植胚におけるDNAのメチル化レベルの検討 (2007)
  • Author(s): 岩本太作, 笠松礼, 立溝篤宏, 谷口俊仁, 中本善之, 谷口俊仁, 出田篤司, 浦川真実, 青柳敬人、細井美彦, 松本和也, 入谷明, 佐伯和弘
  • Journal Title: 近畿大学先端技術総合研究所紀要 12 Pages: 25-31
  • Peer Reviewed
• [Journal Article] Effect of embryonic cell cycle of nuclear donor embryos on the efficiency of nuclear transfer in Japanese black cattle. (2007)
  • Author(s): Kishi M, Takakura R, Nagao Y, Saeki K, Y Takahashi
  • Journal Title: Zygote 15 Pages: 165-171
  • Peer Reviewed
• [Journal Article] Early development in utero of bovine nuclear transfer embryos using early G1 and G0 phase cells. (2007)
  • Author(s): Ideta A, Urakawa M, Aoyagi Y, Saeki K
  • Journal Title: Cloning Stem Cells 9 Pages: 571-580
  • Peer Reviewed
• [Journal Article] 初期G1期細胞によるウシ核移植胚におけるDNAのメチル化レベルの検討 (2007)
  • Author(s): 岩本太作, 笠松礼, 立溝篤宏, 谷口俊仁, 中本善之, 谷口俊仁, 出田篤司, 浦川真実, 青柳敬人, 細井美彦, 松本和也, 入谷明, 佐伯和弘
  • Journal Title: 近畿大学先端技術総合研究所紀要 12 Pages: 25-31
• [Journal Article] Early development in utero of bovine nuclear transfer embryos using early G1 and G0 phase cells (2007)
  • Author(s): Ideta A, Urakawa M, Aoyagi Y, Saeki K.
  • Journal Title: Cloning Stem Cells 9 Pages: 571-580
• [Journal Article] 最近の遺伝子改変家畜の現況, とくに植物遺伝子を利用した家畜の脂質の改変 (2006)
  • Author(s): 佐伯和弘
  • Journal Title: 麻布大学雑誌 Vol.11-12 Pages: 91-96
• [Journal Article] 最近の遺伝子改変家畜の現況,とくに植物遺伝子を利用した家畜の脂質の改変 (2006)
  • Author(s): 佐伯和弘
  • Journal Title: 麻布大学雑誌 Vol.11-12 Pages: 91-96
• [Journal Article] Enhancement of histone acetylation by trichostatin A during in vitro fertilization of bovine oocytes affects cell number of the inner cell mass of the resulting blastocysts.
  • Author(s): Ikeda S, Tatemizo A, Iwamoto D, Taniguchi S, Hoshino Y, Amano T, Matsumoto K, Hosoi Y, Iritani A, Saeki K
  • Journal Title: Zygote 17 Pages: 209-215
  • Peer Reviewed
• [Presentation] ポリジメチルシロキサン製マイクロウェルの容積と胚数がウシ胚の初期発生に及ぼす影響 (2010)
  • Author(s): 岩本太作, 加藤暢宏, 谷口俊仁, 松井孝徳, 高橋千明, 中野達也, 庄隼生, 岸昌生, 細井美彦, 松本和也, 入谷明, 佐伯和弘
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 明治大学駿河台キャンパス東京都千代田区
  • Year and Date: 2010-03-29
• [Presentation] 健康に寄与できる組換え動物性食品開発を目指して:植物由来脂肪酸不飽和化酵素遺伝子の哺乳動物での機能的発現 (2010)
  • Author(s): 佐伯和弘
  • Organizer: 第8回脂質工学研究部会講演会
  • Place of Presentation: 大阪市立工業研究所, 大阪市
  • Year and Date: 2010-01-22
• [Presentation] 健康に寄与できる組換え動物性食品開発を目指して:植物由来脂肪酸不飽和化酵素遺伝子の哺乳動物での機能的発現 (2010)
  • Author(s): 佐伯和弘
  • Organizer: 第8回脂質工学研究部会講演会
  • Place of Presentation: 大阪市立工業研究所(大阪市)
  • Year and Date: 2010-01-22
• [Presentation] 無孔性PDMS製マイクロウェルプレートによるヒト体外受精卵の培養 (2009)
  • Author(s): 加藤暢宏, 佐伯和弘, 橋本周, 森本義晴
  • Organizer: 第31回日本バイオマテリアル学会大会
  • Place of Presentation: 京都府民総合交流プラザ京都市
• [Presentation] 微生物由来リノール酸異性化酵素遺伝子(PAISOM)の哺乳動物細胞における発現とPAISOM導入核移植胚の発生 (2009)
  • Author(s): 中野達也, 印藤頼子, 庄隼生, 細井美彦, 松本和也, 入谷明, 佐伯和弘
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 琉球大学, 沖縄県中頭郡西原町
• [Presentation] Modification of fatty acid profiles in bovine adipocytes by introduction of genes for anω-3 fatty acid desaturase and a linoleate isomerase. (2009)
  • Author(s): Saeki K, Indo Y, T Nakano, Suzuki I, Matsumoto K, Hosoi Y, Kinoshita M, Mikami K, Murata N, Iritani A
  • Organizer: World Congress on Oils and Fats and the 28th ISF Congress
  • Place of Presentation: Sydney, Australia
• [Presentation] Comparison of in vivo development during early pregnancy of cloned fetuses derived from bovine fetal fibroblasts at the early G1 and G0 phases. (2009)
  • Author(s): Ideta A, Hayama K, Urakawa M, Tsuchiya K, Nakamura Y, Aoyagi Y, Saeki K
  • Organizer: 2009 Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: San Diego, California, USA
• [Presentation] 微生物由来リノール酸異性化酵素遺伝子(PAISOM)の哺乳動物細胞における発現とPAISOM導入核移植胚の発生 (2009)
  • Author(s): 中野達也
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 琉球大学(沖縄県中頭郡西原町)
• [Presentation] PDMS製マイクロウェルプレートを用いたウシ胚の初期発生に及ぼすウェルの容積の影響 (2009)
  • Author(s): 岩本太作
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 琉球大学(沖縄県中頭郡西原町)
• [Presentation] SCNT胚を用いたウシキメラ胚の作製の検討 (2009)
  • Author(s): 高橋千明
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 琉球大学(沖縄県中頭郡西原町)
• [Presentation] Effects of trichostatin A on DNA methylation in cloned bovine embryos. (2008)
  • Author(s): Iwamoto D, Kishigami S, Taniguchi S, Abe Y, Matsui T, Kasamatsu A, Tatemizo A, Mitani T, Kato H, Matsumoto K, Hosoi Y, Wakayama T, Iritani A, Saeki K
  • Organizer: Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: Denver, CO, USA
• [Presentation] Effects of trichostatin A on gene expression and in-vitro development of bovine embryos cloned from transfected fibroblasts carrying a luciferase gene. (2008)
  • Author(s): Iwamoto D, Kishigami S, Taniguchi S, Matsumoto K, Hosoi Y, Iritani A, Wakayama T, Saeki K
  • Organizer: 16th Int'l Congress on Animal Reproduction
  • Place of Presentation: Budapest, Hungary
• [Presentation] Expression of anω-3 fatty acid desaturase gene from scarlet flax in bovine transgenic embryos cloned from transfected somatic cells. (2008)
  • Author(s): K Saeki, Y Indo, A Tatemizo, I Suzuki, K Matsumoto, Y Hosoi, N Murata, A Iritani
  • Organizer: The 16th International Congress on Animal Reproduction
  • Place of Presentation: in Budapest, Hungary
• [Presentation] Effects of trichostatin A on gene expression and in-vitro development of bovine embryos cloned from transfected fibroblasts carrying a luciferase gene. (2008)
  • Author(s): Iwamoto D, Kishigami S, Taniguchi S, Matsumoto K, Hosoi Y, Iritani A, Wakayama T, Saeki K
  • Organizer: The 16th International Congress on Animal Reproduction
  • Place of Presentation: in Budapest, Hungary
• [Presentation] Production of cloned bovine embryos derived from amniotic cells of pregnant cows. (2008)
  • Author(s): Taniguchi S, Hayashi N, Abe Y, Iwamoto D, Kishigami S, Kishi M, Kato H, Mitani T, Matsumoto K, Hosoi Y, Iritani A, Nagao Y, Saeki K
  • Organizer: Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: Denver, CO, USA
• [Presentation] ポリジメチルシロキサン製マイクロウェルを用いたウシ胚の体外培養の検討 (2008)
  • Author(s): 岩本太作, 松井 孝徳, 中野 達也, 高橋 千明, 谷口 俊仁, 加藤 暢宏, 佐伯 和弘.
  • Organizer: 第15回日本胚移植研究会
  • Place of Presentation: 仙台市
• [Presentation] Successful Culture of Single Bovine Embryos Using Polydimethylsiloxane (PDMS) Micro-Well Plates Cured under LowPressure. (2008)
  • Author(s): K. Saeki, N. Kato, D. Iwamoto, S. Taniguchi.
  • Organizer: 10th World Conference on Animal Production
  • Place of Presentation: Cape Town, South Africa
• [Presentation] Production ofcloned bovine embryos derived fromamniotic cells of pregnant cows (2008)
  • Author(s): Taniguchi, S. Hayashi, N., Abe, Y., Iwamoto, D., Kishigami, S., Kishi, M., Kato, H., Mitani, T., Matsumoto, K., Hosoi, Y., Iritani, A., Nagao, Y., Saeki, K.
  • Organizer: Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: Denver, CO, USA
• [Presentation] Effects of trichostatin A on DNA methylation in cloned bovine embryos (2008)
  • Author(s): Iwamoto, D., Kishigami, S., Taniguchi, S., Abe, Y., Matsui, T., Kasamatsu, A., TatemizoA. Mitani, T., Kato, H., Matsumoto, K., Hosoi, Y., Wakayama, T., Iritani, A. Saeki, K.
  • Organizer: Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: Denver, CO, USA
• [Presentation] ポリジメチルシロキサン製マイクロウェルを用いたウシ胚の体外培養の検討. (2008)
  • Author(s): 岩本太作
  • Organizer: 第15回日本胚移植研究会
  • Place of Presentation: 仙台市
• [Presentation] Effects of trichostatin A on gene expression and in-vitro development of bovine embryos cloned from transfected fibroblasts carrying a luciferase gene. (2008)
  • Author(s): Iwamoto, D
  • Organizer: 16th Int' 1 Congress on Animal Reproduction
  • Place of Presentation: Budapest, Hungary
• [Presentation] Expression of an omega-3 fatty acid desaturase gene from scarlet flax in bovine transgenic embryos cloned from transfected somatic cells. (2008)
  • Author(s): Saeki, K
  • Organizer: 16th Int' 1 Congress on Animal Reproduction
  • Place of Presentation: Budapest, Hungary
• [Presentation] Successful Culture of Single Bovine Embryos Using Polydimethyl siloxane (PDMS) Micro-Well Plates Cured under Low Pressure. (2008)
  • Author(s): K. Saeki
  • Organizer: 10th World Conference on Animal Production
  • Place of Presentation: Cape Town, South Africa
• [Presentation] Expression of transgene in bovine embryos cloned from somatic cells transfected with a humanized FAD3 gene from scarlet flax. (2007)
  • Author(s): Indo Y, Tatemizo A, Suzuki I, Matsumoto K, Hosoi Y, Kinoshita M, Mikami K, Murata N, Iritani A, Saeki K
  • Organizer: A Joint Meeting of the Second Asian Symposium on Plant Lipids & the 20th Japanese Symposium on Plant Lipids. The Second Asian Symposium on Plant Lipids
  • Place of Presentation: Tokyo, Japan
• [Presentation] アマ由来のω3脂肪酸不飽和過酵素遺伝子を導入したウシ筋衛星細胞によるクローン胚の発生 (2007)
  • Author(s): 印藤頼子, 立溝篤宏, 阿部悠季, 佐伯和弘, 松本和也, 細井美彦, 鈴木石根, 木下幹朗, 三上浩司, 村田紀夫, 入谷明
  • Organizer: 第100回日本繁殖生物学会大会
  • Place of Presentation: 東京
• [Presentation] Elevated level of n-3 fatty acids in bovine adipocytes transfected with a humanized FAD3 gene from scarlet flax. (2007)
  • Author(s): Indo Y, Tatemizo A, Suzuki I, Matsumoto K, Hosoi Y, Kinoshita M, Mikami K, Murata N, Iritani A, Saeki K
  • Organizer: 48th International Conference on the Bioscience of Lipids
  • Place of Presentation: Turku, Finland
• [Presentation] Effects of trichostatin A on gene expression and in-vitro development of bovine embryos cloned from transfected fibroblasts carrying a luciferase gene (2007)
  • Author(s): Iwamoto, D, Kishigami, S, Taniguchi, S, Matsumoto, K, Hosoi, Y, Iritani, A, Wakayama, T, Saeki, K.
  • Organizer: 6th Int'l Congress on Animal Reproduction
  • Place of Presentation: Budapest, Hungary
• [Presentation] Elevated level of n-3 fatty acids in bovine adipocytes transfected with a humanized FAD3 gene from scarlet flax (2007)
  • Author(s): Indo Y, et. al.
  • Organizer: The 48th International Conference 0n the Bioscience of Lipids
  • Place of Presentation: Turku,Finland
• [Presentation] Expression of transgene in bovine embryos cloned from somatic cells transfected with a humanized FAD3 gene from scarlet flax (2007)
  • Author(s): Indo Y, et. al.
  • Organizer: The Second Asian Symposium on Plant Lipids
  • Place of Presentation: Tokyo,Japan
• [Presentation] 遺伝子改変家畜の現状、とくに植物遺伝子を利用した家畜の改変 (2006)
  • Author(s): 佐伯和弘
  • Organizer: 第13回日本胚移植研究会大会シンポジウム
  • Place of Presentation: 広島市
• [Patent(Industrial Property Rights)] 脂肪酸不飽和化酵素遺伝子,組換え発現ベクター,脂肪酸不飽和化酵素遺伝子が導入された,非ヒト動物細胞および非ヒト動物 (2006)
  • Inventor(s): 佐伯和弘・印藤頼子・松本和也・細井美彦・入谷明
  • Industrial Property Rights Holder: 近畿大学
  • Industrial Property Number: 2007-227818
  • Acquisition Date: 2006-02-27
• [Patent(Industrial Property Rights)] 脂肪酸不飽和化酵素遺伝子, 組換え発現ベクター, 脂肪酸不飽和化酵素遺伝子が導入された, 非ヒト動物細胞および非ヒト動物
  • Inventor(s): 佐伯 和弘・印藤頼子・松本 和也・細井 美 彦・入谷 明
  • Industrial Property Number: 2007-227818