Studies on interactions between pestiviruses and host innate immune system

• Project/Area Number: 18380174
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: The University of Tokyo
• Principal Investigator: AKASHI Hiroomi The University of Tokyo, 大学院・農学生命科学研究科, 教授 (10334327)
• Co-Investigator(Kenkyū-buntansha): HARASAWA Ryo 岩手大学, 農学部, 教授 (70159101)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,830,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥2,430,000); Fiscal Year 2008: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2007: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2006: ¥7,300,000 (Direct Cost: ¥7,300,000)
• Keywords: 寄生体生物 / 宿主寄生体相互関係 / BVDV / 粘膜病 / ペスチウイルス / アポトーシス / 先天性免疫 / C型肝炎ウイルス / MDBK細胞 / 2本鎖RNA / エボラウイルス / ELISA法 / 疫学調査 / 蛍光抗体法 / 抗コウモリIgG血清 / コウモリ免疫グロブリン / Bウイルス / ヨコセウイルス

Research Abstract

牛ウイルス性下痢・粘膜病の病態解明を目的として、細胞病原性(cp)および非細胞病原性(ncp)牛ウイルス性下痢ウイルス(BVDV)を用いて、宿主因子との相互作用を解析したところ、細胞病原性がアポトーシスによって起こること、ウイルスRNAがアポトーシスの原因であることを見出した。また、いくつかの重要な宿主因子がウイルス複製に関与していることを明かとした。

Research Products

• [Journal Article] Inhibition of sphingosine kinase by Bovine viral diarrhea virus NS3 is crucial for efficient viral replication and cytopathogenesis (2009)
  • Author(s): Yamane, D., Zahoor, M.A., Mohamed, Y.M.,Azab, W., Kato, K., Tohya, Y. and Akashi, H.
  • Journal Title: J.Biol.Chem. 284 Pages: 13648-13659
• [Journal Article] Microarray analysis reveals distinct signaling pathways transcriptionally activated by infection with bovine viral diarrhea virus in different cell types (2009)
  • Author(s): Yamane, D., Zahoor, M.A., Mohamed, Y.M., Kato, K., Tohya, Y. and Akashi, H.
  • Journal Title: Virus Res. 142 Pages: 188-199
• [Journal Article] Microarray analysis reveals distinct signaling pathways tran scriptionally activated by infection with bovine viral diarrhea virus in different cell types. (2009)
  • Author(s): Yamane, D., et al.
  • Journal Title: Virus Research (in press)
  • Peer Reviewed
• [Journal Article] Inhibition of sphingosine kinase bysbovine viral diarrhea virus NS3 is crucial for efficient viral replication and cytop athogenesis. (2009)
  • Author(s): Yamane, D., et al.
  • Journal Title: Journal of Biological Chemistry (in press)
  • Peer Reviewed
• [Journal Article] The relationship between the viral RNA level and upregulation of innate immunity in spleen of cattle persistently infected with bovine viral diarrhea virus (2008)
  • Author(s): Yamane, D., Kato, K., Tohya, Y. and Akashi, H.
  • Journal Title: Vet. Microbiol. 129 Pages: 69-79
• [Journal Article] Genoepidemiological evaluation of Bovine viral diarrhea virus 2 species based on secondary structures in the 5' untranslated region (2008)
  • Author(s): Giangaspero M, Harasawa R, Weber L, Belloli A.
  • Journal Title: J Vet Med Sci. 70 Pages: 571-80
  • NAID: 110006812733
• [Journal Article] Numerical taxonomy of the genus Pestivirus based on palindromic nucleotide substitutions in the 5' untranslated region (2007)
  • Author(s): Giangaspero M, Harasawa R.
  • Journal Title: J. Virol. Methods 146 Pages: 375-388
• [Journal Article] The relationship between the viral RNA level and upregulation of innate immunity in spleen of cattle persistently infected with bovine viral diarrhea virus (2007)
  • Author(s): Yamane D., et. al.
  • Journal Title: Veterinary Microbiology (In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Evidence for pestivirus infection in free-living Japanese serows, Capricornis crispus (2006)
  • Author(s): Harasawa R, Aoyama F, Goryo M, Okada K, Nishimura T, Matsubara K, Tsujimoto T, Giangaspero M
  • Journal Title: Microbiol. Immunol. 50 Pages: 817-821
  • NAID: 10020555009
• [Journal Article] The double-stranded RNA-induced apoptosis pathway is involved in the cytopathogenicity of cytopathogenic bovine viral diarrhea virus (2006)
  • Author(s): Yamane, D., Kato, K., Tohya, Y. and Akashi H.
  • Journal Title: J. Gen. Virol 87 Pages: 2961-70
• [Journal Article] The double-stranded RNA-induced apoptosis pathway is involved in the cytopathogenicity of cytopathic bovine viral diarrhea virus. (2006)
  • Author(s): Yamane, D., et al.
  • Journal Title: Journal of General Virology 87.10 Pages: 2961-2970
• [Presentation] 牛ウイルス性下痢ウイルスによるスフィンゴ脂質代謝制御を介したウイルス複製機構の解析 (2009)
  • Author(s): 山根大典
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 宇都宮
  • Year and Date: 2009-04-02
• [Presentation] スフィンゴシンキナーゼ活性が牛ウイルス性下痢ウイルス複製に与える影響の解析 (2008)
  • Author(s): 山根大典
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2008-10-26
• [Presentation] Production and characterization of monoclonal antibodies against bovine viral diarrhea virus nonstructural 5A protein (2008)
  • Author(s): Zahoor, M. A
  • Place of Presentation: 岡山
  • Year and Date: 2008-10-26
• [Presentation] Interaction of BVDV NS3 with sphingosine kinase 1 is crucial for efficient viral replication and cytopathogenesis (2008)
  • Author(s): Yamane D.
  • Organizer: 15th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: San Antonio, USA
  • Year and Date: 2008-10-07
• [Presentation] Analysis of cellular signalling pathway transcriptionally activated following bovineviral diarrhea virus infections in different cell types using microarray technology (2008)
  • Author(s): Yamane D.
  • Organizer: 7th Pestivirus Symposium
  • Place of Presentation: Uppsala, Sweden
  • Year and Date: 2008-09-17
• [Presentation] Preparation and characterization of monoclonal antibodies against bovine viral diarrhea virus nonstructural protein 5A (2008)
  • Author(s): Zahoor.Muhammad Atif
  • Organizer: 7th ESVV Pestivirus Symposium
  • Place of Presentation: Uppsala, Sweden
  • Year and Date: 2008-09-16
• [Presentation] Preparation and Characterization of monoclonal antibodies against bovine viral diarrhea virus nonstructural protein 5A (2008)
  • Author(s): Zahoor, M. A
  • Organizer: 7th Pestivirus Symposium
  • Place of Presentation: Uppsala, Sweden
• [Presentation] 牛ウイルス性下痢ウイルス非構造蛋白質NS3によるスフィンゴシンキナーゼ活性制御機構の解析 (2007)
  • Author(s): 山根大典
  • Organizer: 第55回日本ウイルス学会
  • Year and Date: 2007-10-23
• [Presentation] 牛ウイルス性下痢ウイルス非構造蛋白質NS3によるスフィンゴシンキナ・ゼ活性制御機構の解析 (2007)
  • Author(s): 山根 大典
  • Organizer: 第55回日本ウイルス学会
  • Place of Presentation: 札幌、北海道
  • Year and Date: 2007-10-23
• [Presentation] 牛ウイルス性下痢ウイルス持続感染牛におけるウイルスRNA蓄積レベルと先天性免疫誘導の関係 (2007)
  • Author(s): 山根大典
  • Organizer: 第144回日本獣医学会
  • Place of Presentation: 江別、北海道
  • Year and Date: 2007-09-03
• [Presentation] 牛ウイルス性下痢ウイルス持続感染牛におけるウイルスRNA蓄積レベルと先天性免疫誘導の関係 (2007)
  • Author(s): 山根 大典
  • Organizer: 第144回日本獣医学会
  • Place of Presentation: 江別・北海道
  • Year and Date: 2007-09-03
• [Presentation] 牛ウイルス性下痢ウイルスによる病態形成に関与する宿主因子の探索 (2006)
  • Author(s): 山根大典
  • Organizer: 第54 回日本ウイルス学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-11-19
• [Book] 動物微生物学 (2008)
  • Author(s): 明石博臣, 原澤亮
  • Publisher: 朝倉書店