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Establishment of diagnosis systems and development of chemotherapeutic drugs for leishmaniasis

Research Project

Project/Area Number 18380178
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionHokkaido University

Principal Investigator

KATAKURA Ken  Hokkaido University, 大学院・獣医学研究科, 教授 (10130155)

Co-Investigator(Kenkyū-buntansha) NONAKA Nariaki  北海道大学, 大学院・獣医学研究科, 講師 (50281853)
MATSUMOTO Jun  北海道大学, 大学院・獣医学研究科, 博士研究員 (70296169)
MORIMOTO Kazuhiro  北海道薬科大学, 薬学部, 教授 (10113135)
Co-Investigator(Renkei-kenkyūsha) NONAKA Nariaki  宮崎大学, 農学部・獣医学科, 准教授 (50281853)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥16,400,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2008: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2006: ¥7,300,000 (Direct Cost: ¥7,300,000)
Keywords人獣共通感染症 / リーシュマニア症 / 診断 / 治療 / 疫学 / 伝播機構 / 媒介昆虫 / クアシノイド / サシチョウバエ / 吸血源動物 / リポソーム製剤 / 輸入イヌリーシュマニア症 / Sergentomyia / Phlebotomus / 皮膚リーシュマニア症 / パキスタン / Leishmania(L.)major / Leishmania(L.)tropica / レコンビナント抗原 / heat shock protein 70 / Leishmania (L.) major / Leishmania (L.) tropica / Leishmania (V.) panamensis
Research Abstract

リーシュマニア症はイヌや齧歯類を保虫宿主としサシチョウバエが媒介する人獣共通原虫性疾患である。本研究では, エクアドルおよびパキスタンの皮膚リーシュマニア症を診断するための抗原を解析した。パキスタン南部の皮膚リーシュマニア症の原因リーシュマニア種を明らかにした。サシチョウバエの種を同定する方法を開発した。サシチョウバエが吸血する動物種を特定した。アジア産薬用植物から抗原虫活性をもつ化合物を分離・精製し、リポソーム製剤を試作した。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (45 results)

All 2009 2008 2007 2006 Other

All Journal Article (26 results) (of which Peer Reviewed: 24 results) Presentation (12 results) Book (4 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Isolation ofanti-babesial compounds from Bruceajavanica, Curcuma xanthorrhiza, andExcoecaria cochinchinensis2009

    • Author(s)
      Yamada K, Subeki, Nabeta K, Yamasaki M,Katakura K, Matsuura H.
    • Journal Title

      Biosci Biotechnol Biochem 73

      Pages: 776-780

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular epidemiology ofleishmaniasis in Asia (focus on cutaneousinfections)2009

    • Author(s)
      Katakura K
    • Journal Title

      Curr Opin Infect Dis 22

      Pages: 126-130

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of efficacy of bruceine A, a naturalquassinoid compound extracted from amedicinal plant, Brucea javanica, for caninebabesiosis.2009

    • Author(s)
      Nakao R, Mizukami C, Kawamura Y, Subeki,Bawm S, Yamasaki M, Maede Y, Matsuura H,Nabeta K, Nonaka N, Oku Y, Katakura K
    • Journal Title

      J Vet Med Sci 71

      Pages: 33-41

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of efficacy of bruceine A, a natural quassinoid compound extracted from a medicinal plant, Brucea javanica, for canine babesiosis2009

    • Author(s)
      Nakao R
    • Journal Title

      Journal of Veterinary Medical Science 71

      Pages: 33-41

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular epidemiology of leishmaniasis in Asia (focus on cutaneous infections)2009

    • Author(s)
      Katakura K
    • Journal Title

      Current Oninion in Infectious Diseases 22

      Pages: 126-130

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro antitrypanosomal activities of quassinoidcompounds from the fruits of a medicinalplant, Brucea javanica2008

    • Author(s)
      Bawm S, Matsuura H, Elkhateeb A, Nabeta K, Subeki, Nonaka N, Oku Y, Katakura K
    • Journal Title

      Vet Parasitol 158

      Pages: 288-294

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-babesial quassionoids from the fruits of Bruceajavanica2008

    • Author(s)
      Elkhateeb A, Yamasaki M, Maede Y, KatakuraK, Nabeta K, Matsuura H
    • Journal Title

      Nat Prod Commun 3

      Pages: 145-148

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polymorphismof cytochrome b gene in Leishmaniaparasites and their relation to types ofcutaneous leishmaniasis lesions in Pakistan2008

    • Author(s)
      Myint CK, Asato Y, Yamamoto Y, Kato H, Bhutto AM, Soomro FR, Memon MZ,Matsumoto J, Maco JD, Oshiro M, KatakuraK, Hashiguchi Y, Uezato H
    • Journal Title

      Jdermatol 35

      Pages: 76-85

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] AnaerobicNADH-fumarate reductase system ispredominant in the respiratory chain ofEchinococcus multilocularis, providing anovel target for the chemotherapy of alveolarechinococcosis2008

    • Author(s)
      Matsumoto J, Sakamoto K, Shinjyo N, Kido Y, Yamamoto N, Yagi K, Miyoshi H, Nonaka N, Katakura K, Kita K, Oku Y
    • Journal Title

      Antmicrob Agents Chemother 52

      Pages: 164-170

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Leishmaniasis in Sindh, Pakistan : outbreakand review of the literature2008

    • Author(s)
      Bhutto AM, Soomro FR, Katakura K
    • Journal Title

      J Pak Assoc Dermatol 18

      Pages: 212-219

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Leishmaniasis in Sindh, Pakistan : outbreak and review of the literature2008

    • Author(s)
      Bhutto AM
    • Journal Title

      Journal of Pakistan Association of Dermatology 18

      Pages: 212-219

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polymorphism of cytochrome b gene in Leishmania parasites and their relation to types of cutaneous leishmaniasis lesions in Pakistan2008

    • Author(s)
      Myint CK
    • Journal Title

      Journal of Dermatology 35

      Pages: 76-85

    • Related Report
      2008 Annual Research Report 2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro antitrypanosomal activities of quassinoid compounds from the fruits of a medicinal plant, Brucea javanica2008

    • Author(s)
      Bawm S
    • Journal Title

      Veterinary Parasitology 158

      Pages: 288-294

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anti-babesial quassionoids from the fruits of Brucea javanica2008

    • Author(s)
      Elkhateeb A
    • Journal Title

      Natural Product Communication 3

      Pages: 145-148

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anaerobic NADH-fumarate reductase system is predominant in the respira tory chain of Echinococcus multilocularis, providing anovel target for the chemotherapy of alveolar echinococcosis2008

    • Author(s)
      Matsumoto J
    • Journal Title

      Antmicrobial Agents and Chemotherapy 52

      Pages: 164-170

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial DNA phylogeography of the red fox Vulpesvulpes in northern Japan2007

    • Author(s)
      Inoue T, Nonaka N, Mizuno A, Morishima Y, Sato H, Katakura K, Oku Y
    • Journal Title

      Zool Science 24

      Pages: 1178-1186

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Screening of Indonesian medicinal plant extracts for anti-babesial activity and isolation of new quassinoids from Brucea javanica2007

    • Author(s)
      Subeki, Matsuura H, Takahashi K, Nabeta K, Yamasaki M, Maede Y, Katakura K
    • Journal Title

      J nat Prod 70

      Pages: 1654-1657

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-babesial compounds fromCurcuma xanthorrhiza2007

    • Author(s)
      Matsuura H, Nomura S, Subeki, Yamada K,Yamasaki M, Yamato O, Maede Y, KatakuraK, Trimurningsih, Chairul, Yoshihara T,Nabeta K
    • Journal Title

      Nat Prod Res 21

      Pages: 328-33

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-babesial compounds from Rosa damascena2007

    • Author(s)
      Elkhateeb A, Matsuura H, Yamasaki M, Maede Y, Katakura K, Nabeta K
    • Journal Title

      Nat Prod Commun 2

      Pages: 765-769

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-babesial compounds from Berberis vulgaris2007

    • Author(s)
      Elkhateeb A, Yamada K, Takahashi K, Matsuura H, Yamasaki M, Maede Y, KatakuraK, Nabeta K
    • Journal Title

      Nat Prod Commun 2

      Pages: 173-175

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Screening of Indonesian medicinal plant extracts for anti-babesial activity and isolation of new quassinoids from Brucea javanica2007

    • Author(s)
      Subeki,
    • Journal Title

      Journal of Natural Products 70

      Pages: 1654-1657

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement ofsplenic glucose metabolism during acutemalarial infectio n: correlation of findings ofFDG-PET imaging with pathological changesin a primate model of severe human malaria2006

    • Author(s)
      Kawai S, Ikeda E, Sugiyama M, Matsumoto J, Higuchi T, Zhang H, Khan N, Tomiyoshi K,Inoue T, Yamaguchi H, Katakura K, Endo K, Matsuda H, Suzuki M
    • Journal Title

      Am J Trop Med Hyg 74

      Pages: 353-360

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multilocus enzyme electrophoresis andcytochrome b gene sequencing-basedidentification of Leishmania isolates fromdifferent loci of cutaneous leishmaniasis inPakistan2006

    • Author(s)
      Marco JD, Bhutto AM, Soomro FR, BalochJH, Barroso PA, Kato H, Uezato H, KatakuraK, Korenaga M, Nonaka S, Hashiguchi Y
    • Journal Title

      Am J Trop Med Hyg 75

      Pages: 261-266

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A canine case of Leishmania infantum infectionin Japan2006

    • Author(s)
      Namikawa K, Watanabe M, Lynch J, SugakiY, Kitai T, Sunaga F, Katakura K, Ogata M
    • Journal Title

      Jpn J Vet Dermatol 12

      Pages: 11-15

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multilocus enzyme electrophoresis and cytochrome b gene sequencing-based identification of Leishmania isolates from different loci of cutaneous leishmaniasis in Pakistan2006

    • Author(s)
      Marco, JD
    • Journal Title

      American Journal of Tropical Medicine and Hygiene 75

      Pages: 261-266

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A canine case of Leishmania infantum infection in Japan.2006

    • Author(s)
      Namikawa, K
    • Journal Title

      Japanese Journal of Veterinary Dermatology 12

      Pages: 11-15

    • Related Report
      2006 Annual Research Report
  • [Presentation] Diagnosis andmolecular epidemiology of cutaneousleishmaniasis in Ecuador and Pakistan2008

    • Author(s)
      Katakura K, Kawamura Y, Nakao R, AoshimaK, Tiwananthagorn S, Nonaka N, Oku Y, Uezato H, Kato H, Bhutto AM, Baloch JH, Soomro FR, Hashiguchi Y
    • Organizer
      17^<th> International Congress for Tropical Medicine and Malaria
    • Place of Presentation
      韓国
    • Year and Date
      2008-09-30
    • Related Report
      2008 Final Research Report
  • [Presentation] Antibabesial and antitrypanosomal activity of quassinoidcompounds isolated from the medicinal plantBrucea javanica2008

    • Author(s)
      Bawm S, Subeki, Nakao R, Matsuura H, Elkhateeb A, Takahashi K, Nabeta K, NonakaN, Oku Y, Katakura K
    • Organizer
      17^<th> International Congress for Tropical Medicine and Malaria
    • Place of Presentation
      韓国
    • Year and Date
      2008-09-30
    • Related Report
      2008 Final Research Report
  • [Presentation] Antibabesial and antitrypanosomal activity of quassinoid compounds from the medicinal plant, Brucea javanica2008

    • Author(s)
      Bawm S
    • Organizer
      XVII International Congress for Tropical Medicine and Malaria
    • Place of Presentation
      International Convention Center Jeju, Korea
    • Year and Date
      2008-09-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] Diagnosis and molecular epidemiology of cutaneous leishmaniasis in Ecuador and Pakistan2008

    • Author(s)
      Katakura K
    • Organizer
      XVII International Congress for Tropical Medicine and Malaria
    • Place of Presentation
      International Convention Center Jeju, Korea
    • Year and Date
      2008-09-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] パキスタンの皮膚リーシュマニア症流行地におけるサシチョウバエの分子疫学調査2008

    • Author(s)
      川村悠太、中尾亮、青島圭介、小寺亜由美、Baloch JH、Bhutto MK、Soomro FR、Bhutto AM、野中成晃、奥祐三郎、片倉賢
    • Organizer
      第77回日本寄生虫学会
    • Place of Presentation
      長崎市
    • Year and Date
      2008-04-03
    • Related Report
      2008 Final Research Report
  • [Presentation] In vitro antitrypanosomal activity of quassinoid compounds isolated from the medicinal plantBrucea javanica2008

    • Author(s)
      Bawm S, Subeki, Nakao R, Matsuura H,Takahashi K, Nabeta K, Matsumoto J,Nonaka N, Oku Y, Katakura K
    • Organizer
      第77回日本寄生虫学会
    • Place of Presentation
      長崎市
    • Year and Date
      2008-04-03
    • Related Report
      2008 Final Research Report
  • [Presentation] パキスタンの皮膚リーシュマニア症流行地におけるサシチョウバエの分子疫学調査2008

    • Author(s)
      片倉賢
    • Organizer
      第77回日本寄生虫学会
    • Place of Presentation
      長崎ブリックホール, 長崎市
    • Year and Date
      2008-04-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] In vitro antitrypanosomal activities of quassinoid compounds isolated from the medicinal plant, Brucea javanica2008

    • Author(s)
      Bawm S
    • Organizer
      第77回日本寄生虫学
    • Place of Presentation
      長崎ブリックホール, 長崎市
    • Year and Date
      2008-04-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] 日本国内で発症した犬リーシュマニア症2007

    • Author(s)
      川村悠太、吉川功、片倉賢
    • Organizer
      第144回日本獣医学会
    • Place of Presentation
      江別市
    • Year and Date
      2007-09-03
    • Related Report
      2008 Final Research Report
  • [Presentation] 日本国内で発症した犬リーシュマニア症2007

    • Author(s)
      川村悠太
    • Organizer
      日本獣医寄生虫学会
    • Place of Presentation
      江別市,酪農学園大学
    • Year and Date
      2007-09-02
    • Related Report
      2007 Annual Research Report
  • [Presentation] 薬用植物由来天然化合物のbruceine A のイヌ・バベシア症に対する治療効果2007

    • Author(s)
      中尾亮、水上智秋、川村悠太、Subeki、BawmS、山崎真大、前出吉光、松浦英幸、高橋公咲、鍋田憲助、片倉賢
    • Organizer
      第76回日本寄生虫学会
    • Place of Presentation
      吹田市
    • Year and Date
      2007-03-29
    • Related Report
      2008 Final Research Report
  • [Presentation] パキスタンにおける皮膚リーシュマニア症の血清診断と抗原分析2006

    • Author(s)
      谷口祥介、長内由利子、松本淳、上里博、加藤大智、Bhutto AM、 Soomro FR、MarcoJD、Barroso PA、橋口義久、片倉賢
    • Organizer
      第142回日本獣医学会
    • Place of Presentation
      山口市
    • Year and Date
      2006-09-22
    • Related Report
      2008 Final Research Report
  • [Book] ズーノーシスハンドブック(岸本寿男、山田章雄監修)2009

    • Author(s)
      片倉賢
    • Total Pages
      919
    • Publisher
      メディカルサイエンス
    • Related Report
      2008 Final Research Report
  • [Book] ズーノーシスハンドブック2009

    • Author(s)
      岸本寿男、山田章雄(監修)
    • Publisher
      メディカルサイエ, ンス(印刷中)
    • Related Report
      2008 Annual Research Report
  • [Book] 主要症状を基礎にした犬の臨床(前出吉光監修)2007

    • Author(s)
      片倉賢
    • Total Pages
      232
    • Publisher
      ディーリマン
    • Related Report
      2008 Final Research Report
  • [Book] 主要症状を基礎にした犬の臨床2007

    • Author(s)
      片倉 賢(前出吉光監修)
    • Total Pages
      919
    • Publisher
      デーリィマン社
    • Related Report
      2007 Annual Research Report 2006 Annual Research Report
  • [Remarks]

    • URL

      http://www.vetmed.hokudai.ac.jp/parasitology01.html

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.vetmed.hokudai.ac.jp/parasitology01.html

    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] 抗バベシア剤および新規クアシノイド誘導体2006

    • Inventor(s)
      スベキ, 前出吉光, 片倉賢, 松浦英幸, 鍋田憲助
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2006-048411
    • Filing Date
      2006-02-24
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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