Functional analysis of a novel UCP molecule in heat-production in skunk cabbage

• Project/Area Number: 18380196
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Iwate University
• Principal Investigator: ITO Kikukatsu Iwate University, 農学部, 教授 (50232434)
• Co-Investigator(Kenkyū-buntansha): YOSHIKAWA Nobuyuki 岩手大学, 農学部, 教授 (40191556)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,020,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥2,520,000); Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2007: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2006: ¥6,100,000 (Direct Cost: ¥6,100,000)
• Keywords: 発熱植物 / 呼吸 / ミトコンドリア / 脱共役活性 / シアン耐性経路 / ザゼンソウ / 植物の熱産生 / 脱共役蛋白質 / ユビキノン / シアン耐性呼吸酵素 / 肉穂花序 / ウイルス感染 / 呼吸制御 / 脱共役タンパク質 / SfUCPB

Research Abstract

わが国の寒冷地に自生するザゼンソウは氷点下を含む外気温の変動にも関わらず発熱によりその花器の体温をほぼ一定に維持することができる発熱植物である。本研究においては、ザゼンソウの発熱現象にミトコンドリアにおける脱共役蛋白質(UCP)がシアン耐性呼吸酵素(AOX)とともに機能していることを明らかにすることができた。本研究の成果は、ザゼンソウのみならずハス等の他の発熱植物の熱産生メカニズムの理解にも大きく貢献するものと考えられる。

Research Products

• [Journal Article] In vivo redox state of the ubiquinone pool in the spadices of the thermogenic skunk cabbage, Symplocarpus renifolius (2009)
  • Author(s): Kamata, T., Matsukawa, K., Kakizaki, Y., and Ito, K.
  • Journal Title: Journal of Plant Research (in press)
  • NAID: 10027217071
  • Peer Reviewed
• [Journal Article] Two Cys or not two Cys, that is the question? Alternative oxidase in the thermogenic Nelumbo nucifera (2009)
  • Author(s): Nicole, G., Onda, Y., Kakizaki, Y.,Ito, K.,Watling, J. and Sharon, R.
  • Journal Title: Plant Physiology (in press)
  • Peer Reviewed
• [Journal Article] Functional expression of plant alternative oxidase decreases antimycin A-induced reactive oxygen species production in human cells (2009)
  • Author(s): Matsukawa, K., Kamata, T., and Ito, K.
  • Journal Title: FEBS Letters 583 Pages: 148-152
  • Peer Reviewed
• [Journal Article] Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes (2009)
  • Author(s): Igarashi, A., Yamagata, K., Sugai, T., Takahashi, Y., Sugawara, E., Tamura, A., Yaegashi, H., Yamagishi, N., Takahashi, T., Isogai, H., and Yoshikawa, N.
  • Journal Title: Virology 386 Pages: 407-416
  • Peer Reviewed
• [Journal Article] Two Cys or not two Cys, that is the Question? Alternative oxidase in the thermogenic Nelumbo nucifera (2009)
  • Author(s): Nicole, G., Onda, Y., Kakizaki, Y., Ito, K., Watling, J. and Sharon, R.
  • Journal Title: Plant Physiology (In press)
  • Peer Reviewed
• [Journal Article] In vivo redox state of the ubiquinone pool in the spadices of the thermogenic skunk cabbage, Sygplocarpus rehifolius (2009)
  • Author(s): Kamata, T., Matsukawa, K., Kakizaki, Y. and Ito. K.
  • Journal Title: Journal of Plant Research (In press)
  • Peer Reviewed
• [Journal Article] Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurliits, and legumes (2009)
  • Author(s): Igarashi, A., Yamagata, K., Sugai, T. Takahashi, Y., Sugawara, E., Tamura, A. Yaegashi, H., Yamagishi N., Takahashi, T., Isogai, M., and Yoshikawa, N.
  • Journal Title: FEBS Letters 386 Pages: 407-416
  • Peer Reviewed
• [Journal Article] Functional coexpression of the mitochondrial alternative oxidase and uncoupling protein underlies thermoregulation in the thermogenic florets of skunk cabbage (2008)
  • Author(s): Onda, Y. Kato, Y. Abe, Y. Ito, T.Morohashi, M. Ito, Y. Ichikawa, M., Matsukawa, K. Kakizaki, Y. Koiwa, H., and Ito, K.
  • Journal Title: Plant Physiology 146 Pages: 636-645
  • Peer Reviewed
• [Journal Article] Functional coexpression of the mitochondrial alternative oxidase and uncoupling protein underlies thermoregulation in the thermogenic florets of skunk cabbage. (2008)
  • Author(s): Y. Onda, Y. Kato, Y. Abe, T. lto, M. Morohashi, Y. Ito, M. Ichikawa, K. Matsukawa, Y. Kakizaki, H. Koiwa, K. Ito
  • Journal Title: Plant Physiology 146 Pages: 636-645
  • Peer Reviewed
• [Journal Article] Pyruvate-sensitive AOX exists as a non-covalently associated dimer in the homeothermic spadix of the skunk cabbage, Symplocarpus renifolius (2007)
  • Author(s): Onda,Y., Kato, Y., Abe, Y., Ito, T., Ito-Inaba, Y., Morohashi, M., Ito, Y., Ichikawa, M., Matsukawa, K., Otsuka, M. Koiwa, H., and K. Ito.
  • Journal Title: FEBS Letters 581 Pages: 5852-5825
  • Peer Reviewed
• [Journal Article] 発熱する植物たち-サーモカメラが捉えた発熱現象- (2007)
  • Author(s): 恩田義彦, 伊藤菊一
  • Journal Title: バイオサイエンスとインダストリー 11 Pages: 8-9
  • NAID: 10019803352
• [Journal Article] The combinations of the two amino acids(Ala40 and Phe75 or Ser40 and Tyr75)in the coat protein of apple chlorotic leaf spot virus are crucial for infectivity (2007)
  • Author(s): Yaegashi, H.,Isogai, M., Tajima, H., Sano, T., and Yoshikawa, N.
  • Journal Title: Journal of General Virology 89 Pages: 2611-2618
  • Peer Reviewed
• [Journal Article] Analysis of spatial distribution of two virus populations differently labeled with cyan and yellow fluorescent proteins in co-infected plants (2007)
  • Author(s): Takahashi, T.,Sugawara, T., Yamatsuta, T., Isogai, M., Natsuaki, T., and Yoshikawa, N.
  • Journal Title: Phytopathology 97 Pages: 1200-1206
  • Peer Reviewed
• [Journal Article] Cheravirus and Sadwavirus : two unassigned genera of plant positivesense single-stranded RNA viruses formerly considered atypical members of the genus Nepovirus(family Comoviridae) (2007)
  • Author(s): Le Gall, O., Sanfacon, H., Ikegami, M., Iwanami, T.,Jones, T., Karasev, A., Lehto, K., Wellink, J., Wetzel, T., and Yoshikawa, N.
  • Journal Title: Archives of Virology 152 Pages: 1767-1774
  • Peer Reviewed
• [Journal Article] Characterization of virus-induced gene silencing in tobacco plants infected with Apple latent spherical virus (2007)
  • Author(s): Yaegashi, H.,Yamatsuta, T., Takahashi, T.,Li, C., Isogai, M., Kobori, T., Ohki, S., and Yoshikawa, N.
  • Journal Title: Archives of Virology 152 Pages: 1839-184
  • Peer Reviewed
• [Journal Article] The multifunctional roles of Apple chlorotic leaf spot virus 50KP movement protein (2007)
  • Author(s): Isogai, M., Yaegashi, H., and Yoshikawa, N.
  • Journal Title: Plant viruses 1 Pages: 135-141
  • NAID: 120002647928
  • Peer Reviewed
• [Journal Article] Pyruvate-sensitive AOX exists as a non-covalently associated dimer in the homeothermic spadix of the skunk cabbage, Symplocarpus renifo-lius. (2007)
  • Author(s): Y. Onda, Y. Kato, Y. Abe, T. Ito, Y. Ito-lnaba, M. Morohashi, Y. Ito, M. Ichikawa, K. Matsukawa, M. Otsuka, H. Koiwa, K. Ito
  • Journal Title: FEBS Letters 581 Pages: 5852-5858
  • Peer Reviewed
• [Journal Article] Modeling of the thermoregulation system in the skunk cabbage: Sym-plocarpus foetidus (2007)
  • Author(s): K. Takahashi, T. Itc, T. Endo, S. Chiba, K. Ito, H. Oasada
  • Journal Title: Physical Review E 76
  • NAID: 120005478529
  • Peer Reviewed
• [Journal Article] 発熱する植物たち -サーモカメラが捉えた発熱現象- (2007)
  • Author(s): 恩田義彦, 伊藤菊一
  • Journal Title: バイオサイエンスとインダストリー 11 Pages: 89-89
  • NAID: 10019803352
• [Journal Article] The combinations of the two amino acids(Ala40 and Phe75 or Ser40 and Tyr75)in the coat protein of apple chlorotic leaf spot virus are crucial for infectivity. (2007)
  • Author(s): Yaegashi, H., Isogai, M., Tajima, H., Sano, T., and Yoshikawa, N.
  • Journal Title: J. Gen. Virol. 89 Pages: 2611-2618
  • Peer Reviewed
• [Journal Article] Analysis of spatial distribution of two virus populations differently labeled with cyan and yellow fluorescent proteins in co-infected plants. (2007)
  • Author(s): Takahashi, T., Sugawara, T., Yamatsuta, T., Isogai, M., Natsuaki, T., and Yoshikawa, N.
  • Journal Title: Phytopathology 97 Pages: 1200-1206
  • Peer Reviewed
• [Journal Article] Cheravirusand Sadwavirus: two unassigned genera of plant positive-sense single-stranded RNA viruses formerly considered atypical members of the genus Nepovirus(family Comoviridae). (2007)
  • Author(s): Le Gall, O., Sanfacon, H., Ikegami, M., Iwanami, T., Jones, T., Karasev, A., Lehto, K., Wellink, J., Wetzel, T., and Yoshikawa, N
  • Journal Title: Arch. Virol. 152 Pages: 1767-1774
  • Peer Reviewed
• [Journal Article] Characterization of virus-induced gene silencing in tobacco plants infected with Apple latent spherical virus. (2007)
  • Author(s): Yaegashi, H., Yamatsuta, T., Takahashi, T., Li, C., Isogai, M., Kobori, T., Ohki, S., and Yoshikawa, N.
  • Journal Title: Arch. Virol. 152 Pages: 1839-1849
  • Peer Reviewed
• [Journal Article] The multifunctional roles of Apple chlorotic leaf spot virus 50KP movement protein. (2007)
  • Author(s): Isogai, M., Yaegashi, H., and Yoshikawa, N.
  • Journal Title: Plant viruses 1 Pages: 135-141
  • NAID: 120002647928
  • Peer Reviewed
• [Journal Article] 発熱植物ザゼンソウに見出された非線形体温制御システム (2007)
  • Author(s): 伊藤孝徳, 伊藤菊一
  • Journal Title: ブレインテクノニュース 119 Pages: 1-8
• [Journal Article] Functional analysis of skunk cabbage SfUCPB, a unique uncoupling protein lacking the fifth transmembrane domain, in yeast cells (2006)
  • Author(s): Ito, K., Matsukawa, K., and Kato, Y.
  • Journal Title: Biochemical and Biophysical Research Communications 349 Pages: 383-390
  • Peer Reviewed
• [Journal Article] ザゼンソウの発熱制御システム (2006)
  • Author(s): 伊藤孝徳, 伊東靖子, 伊藤菊一
  • Journal Title: 化学と生物 44 Pages: 225-232
  • NAID: 10017316773
• [Journal Article] Functional analysis of skunk cabbage SfUCPB, a unique uncoupling protein lacking the fifth transmembrane domain, in yeast cells. (2006)
  • Author(s): Ito, K., Matsukawa, K., Kato, Y.
  • Journal Title: Biochem. Biophys. Res. Comm. 349 Pages: 383-390
• [Journal Article] リンゴ小球形潜在ウイルスの感染・移行とRNAサイレンシングの誘導 (2006)
  • Author(s): 吉川信幸, 山蔦 翼, 佐々木伸浩, 五十嵐亜紀, 山形広輔, 八重樫 元, 高橋 翼, 磯貝雅道
  • Journal Title: 植物感染生理談話会論文集 42 Pages: 107-116
• [Presentation] ガラクトース培地で培養した哺乳動物細胞におけるアンチマイシンAによって生じる活性酸素は、シアン耐性呼吸酵素の機能的発現により軽減できる (2008)
  • Author(s): 松川和重, 伊藤菊一
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] ガラクトース培地で培養した哺乳動物細胞におけるアンチマイシンAによって生じる活性酸素は、シアン耐性呼吸酵素の機能的発現により軽減できる (2008)
  • Author(s): 松川和重, 伊藤菊一
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Mitochondrial cyanide-resIstant respiratory pathway alleviates generation of reactive oxygen species by complex HI inhibitor, antimycin A, in human cells (2008)
  • Author(s): Matsukawa, K., Kamata, T., and Ito, K.
  • Organizer: First International AOX Symposium
  • Place of Presentation: Evora, Portugal
  • Year and Date: 2008-10-26
• [Presentation] Mitochondrial cyanide-resistant respiratory pathway alleviates generation of reactive oxygen species by complex III inhibitor, antimycin A, in human cells (2008)
  • Author(s): Matsukawa, K., Kamata, T., and Ito, K.
  • Organizer: First International AOX Symposium, Evora
  • Place of Presentation: Portugal
  • Year and Date: 2008-10-22
• [Presentation] The co-expression of the mitochondrial SfAOX and SfUCPb genes underlies the mechanism of homeothermic regulation in thermogenic florets of the skunk cabbage, Symplocarpus foetidus (2007)
  • Author(s): Onda, Y., and Ito, K.
  • Organizer: 8th International Plant Cold Hardiness Seminar
  • Place of Presentation: Saskatoon, Canada
  • Year and Date: 2007-08-05
• [Presentation] 寒冷環境におけるザゼンソウの発熱制御システム (2007)
  • Author(s): 伊藤菊一
  • Organizer: 平成19年度日本生物工学会北日本支部仙台シンポジウム「生物のユニークな環境適応戦略-その分子機構と応用ポテンシャル-」
  • Place of Presentation: 仙台
  • Year and Date: 2007-07-19
• [Presentation] 寒冷環境におけるザゼンソウの発熱制御システム (2007)
  • Author(s): 伊藤菊一
  • Organizer: 平成19年度日本生物工学会北日本支部仙台シンポジウム「生物のユニークな環境適応戦略 -その分子機構と応用ポテンシャル-」
  • Place of Presentation: 仙台市戦災復興記念館
  • Year and Date: 2007-07-19
• [Presentation] Thermoregulation system in the skunk cabbage, Symplocarpus foetidus (2007)
  • Author(s): K. Takahashi, T. Ito, T. Endo, S. Chiba, K. Ito, H. Oasada
  • Organizer: Dynamics Days Europe 2007
  • Place of Presentation: Loughborough University, UK
• [Presentation] The co-expression of the mitochondrial SfAOX and SfUCPb genes underlies the mechanism of homeothermic regulation in thermogenic florets of the skunk cabbage, Symplocarpus foetidus. (2007)
  • Author(s): Y. Onda, K. Ito
  • Organizer: 8th International Plant Cold Hardiness Seminar
  • Place of Presentation: University of Saskatoon, Canada
• [Book] 植物まるかじり叢書 (2008)
  • Author(s): 伊藤菊一
  • Publisher: 花はなぜ咲くの?
• [Book] 花はなぜ咲くの? 植物まるかじり叢書 (2008)
  • Author(s): 伊藤菊一(分担執筆)
  • Total Pages: 2
  • Publisher: 化学同人
• [Remarks] 伊藤菊一第2回ザゼンソウシンポジウム演題 : ザゼンソウの発熱現象 -基礎から応用まで-2009年2月28日,今津
• [Remarks] 伊藤菊一第1回ザゼンソウシンポジウム演題 : 発熱植物ザゼンソウの不思議2008年2月28日,今津
• [Remarks]
  • URL: http://news7al.atm.iwate-u.ac.jp/zazensou/main/achievement.html