Mechanism of light sensing and signaling mediated by phytochrome and its interacting proteins in plants

• Project/Area Number: 18380200
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Kyoto University
• Principal Investigator: KOHCHI Takayuki Kyoto University, 大学院・生命科学研究科, 教授 (40202056)
• Co-Investigator(Kenkyū-buntansha): 大和 勝幸 京都大学, 生命科学研究科, 助教 (50293915)
• Co-Investigator(Renkei-kenkyūsha): YAMATO T.Katsuyuki 京都大学, 大学院・生命科学研究科, 助教 (50293915)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥17,250,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥2,550,000); Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2007: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2006: ¥6,200,000 (Direct Cost: ¥6,200,000)
• Keywords: 発生・分化制御 / タンパク質間相互作用 / 植物光信号伝達 / 光受容体 / フィトクロム / 環境応答 / シロイヌナズナ / タンパク質相互作用 / 光信号伝達 / 光背センシング / 光形態形成 / 光受容 / 遺伝子発現制御 / 信号伝達 / 遺伝子発現

Research Abstract

The novel zinc finger proteins were identified by protein interaction screening with phytochromes. The mutants having T-DNA insertion in the corresponding genes showed late flowering phenotype. The genetic analysis indicated the genes regulated FT expression as downstream factors of phytochrome B. The studies of temporal and spatial expression, phenotypic complementation with the modified genes indicated the proteins functioned in the nucleus in vascular tissue. Our finding gives insight on the link between light environment and development in growth control.

Research Products

• [Journal Article] Characterization of the heme oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions. (2010)
  • Author(s): Gisk, B., Yasui, Y., Kohchi, T., Frankenberg-Dinkel, N.
  • Journal Title: Biochem. J. 425 Pages: 425-434
  • Peer Reviewed
• [Journal Article] Characterization of the heme oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions (2010)
  • Author(s): Gisk, B., Yasui, Y., Kohchi, T., Frankenberg-Dinkel, N.
  • Journal Title: Boichem.J. 425 Pages: 425-434
  • Peer Reviewed
• [Journal Article] Crystal structure of red chlorophyll catabolite reductase: enlargement of the ferredoxin-dependent bilin reductase family. (2009)
  • Author(s): Sugishima, M., Kitamori, Y., Noguchi, M., Kohchi, T., Fukuyama, K.
  • Journal Title: J. Mol. Biol. 389 Pages: 376-387
  • Peer Reviewed
• [Journal Article] Crystal structure of red chlorophyll catabolite reductase : enlargement of the ferredoxin-dependent bilin reductase family (2009)
  • Author(s): Sugishima, M., Kitamori, Y., Noguchi, M., Kohchi, T., Fukuyama, K.
  • Journal Title: J.Mol.Biol. 389 Pages: 367-387
  • Peer Reviewed
• [Journal Article] Crystal structure of red chlorophyll catabolite reductase : nlargement of the ferredoxin-dependent bilin reductase family (2009)
  • Author(s): Sugishima, M., Kitamori, Y., Noguchi, M., Kohchi, T. and Fukuyama, K.
  • Journal Title: J. Mol. Biol (in press)
  • Peer Reviewed
• [Journal Article] Agrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology. (2008)
  • Author(s): shizaki, K., Chiyoda, S., Yamato, K.T., Kohchi, T.
  • Journal Title: lant Cell Physiol 49 Pages: 1084-1091
  • Peer Reviewed
• [Journal Article] Characterization of the photoactive GAF domain of the CikA homolog (SyCikA, Slr1969) of the cyanobacterium Synechocystis sp. PCC 6803. (2008)
  • Author(s): Narikawa, R., Kohchi, T., Ikeuchi, M.
  • Journal Title: Photochem Photobiol Sci. 7 Pages: 1253-1259
  • Peer Reviewed
• [Journal Article] Agrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology (2008)
  • Author(s): Ishizaki, K., Chiyoda, S., Yamato, K.T. and Kohchi, T.
  • Journal Title: Plant Cell Physiol 49 Pages: 1084-1091
  • Peer Reviewed
• [Journal Article] Agrobacterium-mediaied transformation of the haploid liverwort Marchantia polymorpha L. (2008)
  • Author(s): Ishizaki, K., Chiyoda, S., Yamato, K. T., Kohchi, T.
  • Journal Title: Plant Cell Physiol. 49 Pages: 1084-1091
  • Peer Reviewed
• [Journal Article] Direct transformation of the liverwort Marchantia polymorpha L. byparticle bombardment using immature thalli developing from spores. (2008)
  • Author(s): Chiyoda, S., Ishizaki, K., Kataoka, H., Yamato, K. T., Kohchi, T.
  • Journal Title: Plant Cell Rep. 27 Pages: 1467-1473
  • Peer Reviewed
• [Journal Article] Cyanobacteriochrome TePixJ of Thermosynechococcus elongatus harbors phycoviolobilin as a chromophore. (2007)
  • Author(s): Ishizuka, T., Narikawa, R., Kohchi, T., Katayama, M., Ikeuchi, M.
  • Journal Title: Plant Cell Physiol 48 Pages: 1385-1390
  • Peer Reviewed
• [Journal Article] Cyanobacteriochrome TePixJ of Thermosynechococcus elongatus harbors phycoviolobilin as a chromophore (2007)
  • Author(s): Ishizuka, T., Narikawa, R., Kohchi, T., Katayama, M., and Ikeuchi, M.
  • Journal Title: Plant Cell Physiol 48 Pages: 1385-1390
  • Peer Reviewed
• [Journal Article] Simple and efficient plastid transformation system for the liverwort Marchantia polymorpha L. suspension-culture cells (2007)
  • Author(s): Chiyoda, S., Linley, P. J., Yamato, K. T., Fukuzawa, H., Yokota, A., and Kohchi, T.
  • Journal Title: Transgenic Res 16 Pages: 41-49
  • Peer Reviewed
• [Journal Article] Gene organization of the liverwort Y chromosome reveals distinct sex chromosome evolution in a haploid system。 (2007)
  • Author(s): Yamato, K. T, et. al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 6472-6477
  • Peer Reviewed
• [Journal Article] Simple and efficient-plastid transformation system for the liverwort Marchantia Polymorpha L. suspension- (2007)
  • Author(s): Chiyoda, S., et. al.
  • Journal Title: Transgenic Res. 16 Pages: 41-49
  • Peer Reviewed
• [Journal Article] Cyanobacteriochrome TePixJ of Thermosynechococcus elongatus harbors phycoviolobilin as a chromophore. (2007)
  • Author(s): Ishizuka, T., et. al.
  • Journal Title: Plant Cell Physiol. 48 Pages: 1385-1390
  • Peer Reviewed
• [Journal Article] Simple and efficient plastid transformation system for the liverwort Marchantia polymorpha L. suspension-culture cells. (2007)
  • Author(s): Chiyoda, S., Linley, P.J., Yamato, K.T., Fukuzawa, H., Yokota, A., Kohchi, T.
  • Journal Title: Transgenic Res. 16 Pages: 41-49
• [Journal Article] Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli. (2006)
  • Author(s): Mukougawa, K., Kanamoto, H., Kobayashi, T., Yokota, A., Kohchi, T.
  • Journal Title: FEBS Lett. 580 Pages: 1333-1338
  • Peer Reviewed
• [Journal Article] Reconstitution of blue/green reversible photoconversion of a cyanobacterial photoreceptor. PixJ1 in phycocyanobilin producing E. coli. (2006)
  • Author(s): Yoshihara, S., Shimada, T., Matsuoka, D., Zikihara, K., Kohchi, T., Tokutomi, S.
  • Journal Title: Biochem. 45 Pages: 3775-3784
  • Peer Reviewed
• [Journal Article] The molecular basis of heme oxygenase deficiency in the pcd1 mutant of pea. (2006)
  • Author(s): Linley, P.J., Landsberger, M., Kohchi, T., Cooper, J.B., Terry, M.J.
  • Journal Title: FEBS J. 273 Pages: 2594-2606
  • Peer Reviewed
• [Journal Article] Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli (2006)
  • Author(s): Mukougawa, K., Kanamoto, H., Kobayashi, T., Yokota, A., and Kohchi, T.
  • Journal Title: FEBS Lett 580 Pages: 1333-1338
  • Peer Reviewed
• [Journal Article] The molecular basis of heme oxygenase deficiency in the pcd1 mutant of pea (2006)
  • Author(s): Linley, P.J., Landsberger, M., Kohchi, T., Cooper, J.B., Terry, M.J.
  • Journal Title: FEBS J. 273 Pages: 2594-2606
• [Journal Article] Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli (2006)
  • Author(s): Mukougawa, K., Kanamoto, H., Kobayashi, T., Yokota, A., Kohchi, T.
  • Journal Title: FEBS Lett. 580 Pages: 1333-1338
• [Presentation] シロイヌナズナ花成制御におけるVOZ遺伝子の機能解析 (2010)
  • Author(s): 西谷愛依子, 安居佑季子, 硯亮太, 上本允大, 向川佳子, 中井勇介, 中平洋一, 佐藤雅彦, 河内孝之
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2010-03-28
• [Presentation] シロイヌナズナ花成制御におけるVOZ遺伝子の機能解析 (2010)
  • Author(s): 西谷愛依子, 安居佑季子, 硯亮太, 上本允大, 向川佳子, 中井勇介, 中平洋一, 佐藤雅彦, 河内孝之
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学 (東京都)
  • Year and Date: 2010-03-28
• [Presentation] フィトクロムシグナル伝達において機能する花成促進因子VOZの局在解析 (2010)
  • Author(s): 安居佑季子, 西谷亜依子, 上本允大, 硯亮太, 向川佳子, 中井勇介, 中平洋一, 佐藤雅彦, 河内孝之
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本市)
  • Year and Date: 2010-03-19
• [Presentation] フィトクロムシグナル伝達において機能する花成促進因子VOZの局在解析 (2010)
  • Author(s): 安居佑季子, 西谷亜依子, 上本允大, 硯亮太, 向川佳子, 中井勇介, 中平洋一, 佐藤雅彦, 河内孝之
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学 (熊本市)
  • Year and Date: 2010-03-19
• [Presentation] 花成制御に関わるフィトクロム相互作用因子VOZの細胞内局在解析 (2009)
  • Author(s): 安居佑季子, 硯亮太, 西谷亜依子, 向川佳子, 佐藤雅彦, 河内孝之
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2009-03-22
• [Presentation] 苔類ゼニゴケにおける青色光応答の解析 (2009)
  • Author(s): 久保田茜, 小松愛乃, 片岡秀夫, 石崎公庸, 大和勝幸, 河内孝之
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2009-03-22
• [Presentation] 花成制御に関わるフィトクロム相互作用因子VOZの細胞内局在解析 (2009)
  • Author(s): 安居佑季子, 硯亮太, 西谷亜依子, 向川佳子, 佐藤雅彦, 河内孝之
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-22
• [Presentation] シロイヌナズナ転写因子VOZを介した花成制御機構の解析 (2008)
  • Author(s): 安居佑季子、硯亮太、向川佳子、佐藤雅彦、河内孝之
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-22
• [Presentation] シロイヌナズナ転写因子VOZを介した花成制御機構の解析 (2008)
  • Author(s): 安居佑季子、硯亮太、向川佳子、佐藤雅彦、河内孝之
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-20
• [Presentation] 苔類ゼニゴケをモデルとしたフィトクロムシグナル伝達の解析 (2008)
  • Author(s): 片岡秀夫、石崎公庸、大和勝幸、河内孝之
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-20
• [Presentation] Structure-activity studies of phytochrome chromophore in vivo (2008)
  • Author(s): Kohchi, T.
  • Organizer: 4th Asia Oceania Conference on Photobiology
  • Place of Presentation: Varanasi, India
• [Presentation] Structure-activity studies of phytochrome chromophore in plants (2008)
  • Author(s): Kohchi, T.
  • Organizer: 4th Asia Oceania Conference on Photobuology
  • Place of Presentation: Varanasi, India
• [Presentation] シロイヌナズナのジンクフィンガ-タンパク質VOZの花成制御機能の解析 (2007)
  • Author(s): 硯亮太、向川佳子、安居佑季子、光田展隆、佐藤雅彦、河内孝之
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] シロイヌナズナの花成制御に関わるVOZ遺伝子の機能解析 (2007)
  • Author(s): 硯亮太、向川佳子、秋山昌子、光田展隆、佐藤雅彦、河内孝之
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 松山
  • Year and Date: 2007-03-28
• [Presentation] シロイヌナズナ転写因子VOZを介した花成制御機構の解析 (2007)
  • Author(s): 安居佑季子, 硯亮太, 向川佳子, 佐藤雅彦, 河内孝之
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌国際会議場(札幌市)
  • Year and Date: 2007-03-22
• [Presentation] シロイヌナズナの花成制御に関わるVOZ遺伝子の機能解析 (2006)
  • Author(s): 硯亮太, 向川佳子, 秋山昌子, 光田展隆, 佐藤雅彦, 河内孝之
  • Organizer: 第48回日本植物生理学会年会
  • Place of Presentation: 愛媛大学(松山市)
  • Year and Date: 2006-03-23
• [Presentation] シロイヌナズナの転写因子VOZの花成制御への関与 (2005)
  • Author(s): 硯亮太, 向川佳子, 光田展隆, 佐藤雅彦, 河内孝之
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2005-12-06
• [Book] ゼニゴケの培養法 (2009)
  • Author(s): 大和勝幸, 石崎公庸, 河内孝之
  • Publisher: 低温科学(Low Temperature Science)
• [Book] アグロバクテリウムによるゼニゴケ核ゲノム形質転換法 (2009)
  • Author(s): 石崎公庸, 河内孝之
  • Publisher: 低温科学(Low Temperature Science)
• [Book] ゼニゴケの培養細胞と植物体のプラスチド形質転換法 (2009)
  • Author(s): 千代田将大, 河内孝之
  • Publisher: 低温科学(Low Temperature Science)
• [Remarks]
  • URL: http://www.lif.kyoto-u.ac.jp/labs/plantmb/index.php?cID=65