Davelopment of methodology for the preparation of membrane proteins
| Project/Area Number |
18390006
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Chemical pharmacy
|
|---|
| Research Institution | The University of Tokushima |
|---|
Principal Investigator |
OTAKA Akira The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 教授 (20201973)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIGENAGA Akira 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10423394)
|
|---|
| Project Period (FY) |
2006 – 2008
|
| Project Status |
Completed (Fiscal Year 2008)
|
| Budget Amount *help |
¥13,990,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2007: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2006: ¥5,800,000 (Direct Cost: ¥5,800,000)
|
|---|
| Keywords | 不溶性膜タンパク質 / インテイン / エクステイン / ペプチドチオエステル / アシル転位 / スプライシング / アシル転移 |
|---|
| Research Abstract |
極めて溶解性の低い膜タンパク質の化学合成に向けた化学的基盤として、自然界に見出されるタンパク質の自己編集システムであるインテインーエクステイン系に着目した。この系は、基本的に2つのペプチド結合の切断反応と3っのアシル基転移反応から成り立っている。このインテインーエクステイン系の模倣力杯溶性タンパク質合成に有用と考え、系に含まれる基本的反応の模倣を通じて、不溶性タンパク質合成に役立つ幾つかの反応システムを見出し、その応用展開について検討を加えた。
|
Report
(4 results)
Research Products
(98 results)
-
-
-
-
-
[Journal Article] Electrostatically constrained a-helical peptide inhibits replication of HIV-1 resistant to Enfuvirtide2009
Author(s)
H. Nishikawa, S. Nakamura, E. Kodama, S. Ito, K. Kajiwara, K. Izumi, Y. Sakagami, S. Oishi, T. Ohkubo, Y. Kobayashi, A. Otaka, N. Fujii, and M. Matsuoka
-
Journal Title
Int. J. Cell. Biol 41
Pages: 891-899
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
[Journal Article] Identification of minimal sequence for HIV-1 fusion inhibitors2008
Author(s)
H. Nishikawa, S. Oishi, M. Fujita, K. Watanabe, R. Tokiwa, H. Ohno, E. Kodama, K. Izumi, K. Kajiwara, T. Naitoh, M. Matsuoka, A. Otaka, and N. Fujii
-
Journal Title
Bioorg. Med. Chem 20
Pages: 9184-9187
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Specific recognition of the collagen triple helix by chaperone HSP47. II. The HSP47-binding structural motif in collagens and related proteins2006
Author(s)
T. Koide, Y. Nishikawa, S. Asada, C. M. Yamazaki, Y. Takahara, D. L. Homma, A. Otaka, K. Ohtani, N. Wakamiya, K. Nagata, and K. Kitagawa
-
Journal Title
J. Biol. Chem 281
Pages: 11177-11185
Related Report
Peer Reviewed
-
-
[Journal Article] Stereoselective Synthesis of 3,6-Disubstituted-3,6-dihydropyridin-2-ones as Potential Diketopiperazine Mimetics Using Organocopper-Mediated anti-SN2'Reactions and Their Use in the Preparation of Low-Molecule CXCR4 Antagonists2006
Author(s)
Niida, H. Tanigaki, E. Inokuchi, Y. Sasaki, S. Oishi, H. Ohno, H. Tamamura, Z. Wang, S. C. Peiper, K. Kitaura, A. Otaka, and N. Fujii
-
Journal Title
A. J. Org. Chem 71
Pages: 3942-3951
Related Report
Peer Reviewed
-
[Journal Article] α2 Isoform-specific activation of 5'adenosine monophosphate-activated protein kinase by 5-aminoimidazole-4-carboxamide-1-β-D-ribonucleoside at a physiological level activates glucose transport and increases glucose transporter 4 in mouse skeletal muscle2006
Author(s)
M. Nakano, T. Hamada, T. Hayashi, S. Yonemitsu, L. Miyamoto, T. Toyoda, S. Tanaka, H. Masuzaki, K. Ebihara, Y. Ogawa, K. Hosoda, G. Inoue, Y. Yoshimasa, A. Otaka, T. Fushiki, and K. Nakao
-
Journal Title
Metab. Clinic. Experiment 55
Pages: 300-308
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
