Studies on the novel ubiquitinating enzymes involved in fertilization

• Project/Area Number: 18390022
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Nagoya University
• Principal Investigator: SAWADA Hitoshi Nagoya University, 大学院・理学研究科, 教授 (60158946)
• Co-Investigator(Kenkyū-buntansha): HARADA Yoshito 名古屋大学, 大学院・理学研究科, 助教 (50362223)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,100,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥1,800,000); Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2007: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2006: ¥9,300,000 (Direct Cost: ¥9,300,000)
• Keywords: 受精 / 精子 / ユビキチン / プロテアソーム / ユビキチン化酵素 / ホヤ / プロテアーゼ / 酵素 / タンパク質 / 動物 / 発生・分化 / ウニ

Research Abstract

受精は, 生物が種を保存する上で不可欠なプロセスである。受精を成立させるためには、精子は糖タンパク質性の卵保護層(卵黄膜)に精子通過口を開ける必要があり、この溶解物質はライシンと呼ばれている。新口動物におけるライシンは精子プロテアーゼであると考えられているが, その詳細は不明である。我々は、ホヤにおいて、精子の新規ユビキチンープロテアソーム系がライシンとして細胞外で機能することを見いだした。今回は、カタユウレイボヤを用いて、そのユビキチン化酵素の探索を行い、精子/精巣で特異的に発現する本酵素を同定することに成功した。

Research Products

• [Journal Article] Comprehensiveegg coat proteome of the ascidian Ciona intestinalis reveals gamete recognition molecules involved in self-sterility (2009)
  • Author(s): Yamada, L., Saito, T., Taniguchi, H., Sawada, H., and Harada, Y
  • Journal Title: J. Biol. Chem 284(14) Pages: 9402-9410
  • Peer Reviewed
• [Journal Article] Comprehensive egg coat proteome of the ascidian Ciona intestinalis reveals gamete recognition molecules involved in self-sterility. (2009)
  • Author(s): 山田力志, ほか
  • Journal Title: J. Biol. Chem. 284 Pages: 9402-9410
  • Peer Reviewed
• [Journal Article] Mechanism of self-sterility in a hermaphroditic chordate (2008)
  • Author(s): Harada, Y., Takagaki, Y., Sunagawa, M., Saito, T., Yamada, L., Taniguchi, H., and Shoguchi, E., and Sawada, H
  • Journal Title: Science 320(5875) Pages: 548-550
  • Peer Reviewed
• [Journal Article] Allorecognition mechanisms during ascidian fertilization (2008)
  • Author(s): Harada, Y., and Sawada, H
  • Journal Title: Int. J. Dev. Biol 52(5-6) Pages: 637-645
  • Peer Reviewed
• [Journal Article] Egg and sperm recognition systems during fertilization (2008)
  • Author(s): Hirohashi, N., Kamei, N., Kubo, H., Sawada, H., Matsumoto, M., and Hoshi, M
  • Journal Title: Dev. Growth Differ 50(Suppl 1) Pages: 222-238
  • NAID: 10024400182
  • Peer Reviewed
• [Journal Article] Ascidian sperm glycosylphosphatidylinositol-anchored CRISP-like protein as a binding partner for an allorecognizable sperm receptor on the vitelline coat (2008)
  • Author(s): Urayama, S., Harada, Y., Nakagawa, Y., Ban, S., Akasaka, M., Kawasaki, N., and Sawada, H
  • Journal Title: J. Biol. Chem 283(31) Pages: 21725-21733
  • Peer Reviewed
• [Journal Article] 雌雄同体の脊索動物であるホヤの自家不稔性のメカニズム (2008)
  • Author(s): 原田淑人、澤田均
  • Journal Title: 細胞工学 27(9) Pages: 906-907
• [Journal Article] Mechanism of self-sterility in a hermaphroditic chordate. (2008)
  • Author(s): 原田淑人, ほか
  • Journal Title: Science 320 Pages: 548-550
  • Peer Reviewed
• [Journal Article] Allorecognition mechanisms during ascidian fertilization. (2008)
  • Author(s): 原田淑人, 澤田均
  • Journal Title: Int. J. Dev. Biol 52 Pages: 637-645
  • Peer Reviewed
• [Journal Article] Egg and sperm recognition systems during fertilization. (2008)
  • Author(s): 広橋教貴, ほか
  • Journal Title: Dev. Growth Differ. 50
  • Peer Reviewed
• [Journal Article] Ascidian sperm glycosylphosphatidylinositol-anchored CRISP-like protein as a binding partner for an allorecognizable sperm receptor on the vitelline coat (2008)
  • Author(s): 裏山悟司, ほか
  • Journal Title: J. Biol. Client. 283 Pages: 21725-21733
  • Peer Reviewed
• [Journal Article] 雌雄同体の脊索動物であるホヤの自家不稔性のメカニズム (2008)
  • Author(s): 原田淑人, 澤田均
  • Journal Title: 細胞工学 27 Pages: 906-907
• [Journal Article] Mechanism of self-sterility in a hermaphroditic chordate. (2008)
  • Author(s): Harada, Y., Takagaki, Y., Sunagawa, M., Saito, T., Yamada, L., Taniguchi, H., Shoguchi, E., and Sawada, H.
  • Journal Title: Science 320 Pages: 548-550
  • Peer Reviewed
• [Journal Article] Proteins interacting with the ascidian vitelline-coat sperm receptor HrVC70 as revealed by yeast two-hybrid screening (2007)
  • Author(s): Harada, Y., and Sawada, H
  • Journal Title: Mol. Reprod. Dev 74(9) Pages: 1478-1487
  • Peer Reviewed
• [Journal Article] Sperm proteasomes are responsible for the acrosome reaction and sperm penetration of the vitelline envelope during fertilization of the sea urchin Pseudocentrotus depressus (2007)
  • Author(s): Yokota, N., and Sawada, H
  • Journal Title: Dev. Biol 308(1) Pages: 222-231
  • Peer Reviewed
• [Journal Article] Effects of proteasome inhibitors on fertilization of the sea urchin Anthocidaris crassispina (2007)
  • Author(s): Yokota, N., and Sawada, H
  • Journal Title: Biol. Pharm. Bull 30(7) Pages: 1332-1335
  • NAID: 110006342755
  • Peer Reviewed
• [Journal Article] Effects of proteasome inhibitors on fertilization of the sea urchin Anthocidaris crassispina. (2007)
  • Author(s): Yokota, N., and Sawada, H.
  • Journal Title: Biological & Pharmaceutical Bulletin 30 Pages: 1332-1335
  • NAID: 110006342755
  • Peer Reviewed
• [Journal Article] Sperm proteasomes are responsible for the acrosome reaction and sperm penetration of the vitelline envelope during fertilization of the sea urchin Pseudocentrotus depressus. (2007)
  • Author(s): Yokota, N., and Sawada, H.
  • Journal Title: Developmental Biology 308 Pages: 222-231
  • Peer Reviewed
• [Journal Article] Proteins interacting with the ascidian vitelline-coat sperm receptor HrVC70 as revealed by yeast two-hybrid screening. (2007)
  • Author(s): Harada, Y., and Sawada, H.
  • Journal Title: Mol. Reprod. Dev. 308 Pages: 222-231
  • Peer Reviewed
• [Journal Article] Proteins interacting with the ascidian vitelline-coat sperm receptor HrVC70 as revealed by yeast two-hybrid screening. (2007)
  • Author(s): Harada, Y., Sawada, H.
  • Journal Title: Molecular Reproduction and Development (Epub ahead of print)
• [Journal Article] Effects of proteasome inhibitors on fertilization of the sea urchin Anthocidaris crassispina. (2007)
  • Author(s): Yokota, N., Sawada, H.
  • Journal Title: Biological and Pharmaceutical Bulletin (印刷中)
  • NAID: 110006342755
• [Journal Article] Participation of proteasome-associating complex PC500 in starfish oocyte maturation as revealed by monoclonal antibodies (2006)
  • Author(s): Takagi Sawada, M., Tamura T., Mitani, Y., Kaya, M., Ito, G., Hashimoto, H., and Sawada, H
  • Journal Title: Biochem. Biophys. Res. Commun 349(2) Pages: 694-700
  • Peer Reviewed
• [Journal Article] Participation of proteasome-associating complex PC500 in starfish oocyte maturation as revealed by monoclonal antibodies. (2006)
  • Author(s): Sawada, M.T., Tamura, T., Mitani, Y., Kaya, M., Ito, G., Hashimoto, H., Sawada, H
  • Journal Title: Biochem. Biophys. Res. Commun. 349-2 Pages: 694-700
• [Presentation] マボヤプロテアソームの多型と細胞外ソーティング機構 (2008)
  • Author(s): 片岡陽平, 深田均
  • Organizer: 日本分子生物学会・日本生化学会・合同年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] マウス受精での精子プロテアソームの機能解析 (2008)
  • Author(s): 兵藤仁美, ほか
  • Organizer: 日本分子生物学会・日本生化学会・合同年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] マウス受精での精子プロテアソームの機能解析 (2008)
  • Author(s): 兵藤仁美、山下美鈴、河野菜摘子、康宇鎭、兼城歩、澤田均、馬場忠
  • Organizer: 日本分子生物学会年会・日本生化学会大会・合同年会
  • Place of Presentation: 神戸
• [Presentation] マボヤプロテアソームの多型と細胞外ソーティング機構 (2008)
  • Author(s): 片岡陽平、澤田均
  • Organizer: 日本分子生物学会年会・日本生化学会大会・合同年会
  • Place of Presentation: 神戸
• [Presentation] Extracellular roles of sperm proteasomes in deuterostome fertilization (2008)
  • Author(s): Hitoshi Sawada, Yohei Kataoka, Naoto Yokota
  • Organizer: Zomes-V(5^<TH> International Symposium on the COP9 signalosome, Proteasome and eIF3)
  • Place of Presentation: 横浜
• [Presentation] マボヤ精子プロテアーゼAcrosinとSpermosinの相互作用分子の探索 (2008)
  • Author(s): 赤坂茉莉、原田淑人、澤田均
  • Organizer: 日本動物学会
  • Place of Presentation: 福岡
• [Presentation] カタユウレイボヤ精巣で発現しているユビキチン結合酵素E2の解析 (2008)
  • Author(s): 横田直人、原田淑人、澤田均
  • Organizer: 日本動物学会
  • Place of Presentation: 福岡
• [Presentation] カタユウレイボヤ卵黄膜のプロテオーム解析と自己非自己識別分子の同定 (2008)
  • Author(s): 齋藤貴子、山田力志、谷口寿章、原田淑人、澤田均
  • Organizer: 日本動物学会
  • Place of Presentation: 福岡
• [Presentation] Extracellular roles of sperm proteasomes in deuterostome fertilization (2008)
  • Author(s): 厚田均, ほか
  • Organizer: ZOMES V 国際シンポジウム
  • Place of Presentation: 横浜
• [Presentation] マボヤの精子-卵相互作用に関与する精子GPI結合型タンパク質HrUrabinと精子プロテアーゼHrSpermosinの相互作用に関する解析 (2007)
  • Author(s): 赤坂茉莉、中川洋子、原田淑人、澤田均
  • Organizer: 日本分子生物学会・日本生化学会合同年会
  • Place of Presentation: 福岡
  • Year and Date: 2007-12-12
• [Presentation] Extracellular ubiquitin-proteasome system functioning as a vitelline-coat lysin in ascidians and sea urchins. (2007)
  • Author(s): Hitoshi Sawada
  • Organizer: Okazaki Biology Conference, OBC 6 on"Marine Biology"
  • Place of Presentation: 岡崎
  • Year and Date: 2007-12-03
• [Presentation] ホヤ類の受精におけるトリプシン様プロテアーゼの機能解析 (2007)
  • Author(s): 赤坂茉莉、原田淑人、澤田 均
  • Organizer: 日本動物学会
  • Place of Presentation: 弘前
  • Year and Date: 2007-09-20
• [Presentation] 原索動物の受精における自己非自己認識の分子基盤 (2007)
  • Author(s): 澤田 均
  • Organizer: 日本生体防御学会
  • Place of Presentation: 福岡
  • Year and Date: 2007-07-28
• [Presentation] Sperm GPI-anchored glycoprotein HrUrabin is a binding partner for an allorecognizable egg-coat sperm-receptor HrVC70 during fertilization of the ascidian Halocynthia roretzi (2007)
  • Author(s): Hitoshi Sawada, Yoko Nakagawa, Satoshi Urayama, Nana Kawasaki, and Yoshito Harada
  • Organizer: 4th Tunicate Meeting
  • Place of Presentation: Villefranche-sur-mer, France
  • Year and Date: 2007-06-25
• [Presentation] Positional cloning of a gene for the allorecognition protein during fertilization of the ascidian Ciona intestinalis (2007)
  • Author(s): Yoshito Harada, Yuhei Takagaki, Masahiko Sunagawa, Takako Saitoh, and Hitoshi Sawada
  • Organizer: 4th Tunicate Meeting
  • Place of Presentation: Villefranche-sur-mer, France
  • Year and Date: 2007-06-25
• [Presentation] Vitelline-coat protein CiVC100 is closely related to self-sterility in the ascidian Ciona intestinalis (2007)
  • Author(s): Takako Saitoh, Yuhei Takagaki, Yoshito Harada, and Hitoshi Sawada
  • Organizer: 4th Tunicate Meeting
  • Place of Presentation: Villefranche-sur-mer, France
  • Year and Date: 2007-06-25
• [Presentation] マボヤの精子-卵相互作用に関与する精子GPI結合型タンパク質HrUrabinと精子プロテアーゼHrSpermosinの相互作用に関する解析 (2007)
  • Author(s): 赤坂茉莉、中川洋子、原田淑人、澤田均
  • Organizer: 日本分子生物学会年会・日本生化学会大会・合同年会
  • Place of Presentation: 横浜
• [Presentation] Etracellular ubiquitinproteasome system functioning as a vitelline-coat lysin in ascidians and sea urchins (2007)
  • Author(s): Hitoshi Sawada
  • Organizer: Okazaki Biology Conference, OBC 6 on "Marine Biology"
  • Place of Presentation: 岡崎・鳥羽
• [Presentation] LC/MS/MS解析によるカタユウレイボヤ卵黄膜成分の同定 (2007)
  • Author(s): 齋藤貴子、山田力志、原田淑人、谷口寿章、澤田均
  • Organizer: 日本動物学会
  • Place of Presentation: 弘前
• [Presentation] ウニ精子先体胞中のプロテアソームは卵黄膜ライシンとして機能している (2007)
  • Author(s): 横田直人、澤田均
  • Organizer: 日本動物学会
  • Place of Presentation: 弘前
• [Presentation] ホヤ類の受精における精子トリプシン様プロテアーゼの機能解析 (2007)
  • Author(s): 赤坂茉莉、原田淑人、澤田均
  • Organizer: 日本動物学会
  • Place of Presentation: 弘前
• [Presentation] カタユウレイボヤの自家不和合性に関与するアロ認識分子のポジショナルクローニング (2007)
  • Author(s): 原田淑人、高垣裕平、砂川昌彦、澤田均
  • Organizer: 日本動物学会
  • Place of Presentation: 弘前
• [Presentation] 原索動物の受精における自己非自己認識の分子基盤 (2007)
  • Author(s): 澤田均
  • Organizer: 日本生体防御学会学術総会
  • Place of Presentation: 福岡
• [Presentation] Vitelline coat protein CiVC100 is closely related ot self-sterility in the ascidian Ciona intestinalis (2007)
  • Author(s): Takako Saito, Yuhei Takagaki, Yoshito Harada, Hitoshi Sawada
  • Organizer: 4^<th> Tunicate Meeting
  • Place of Presentation: Villefranche-sur-Mer(France)
• [Presentation] Positional cloning of a gene for the allorecognition molecule during fertilization of C. intestinalis (2007)
  • Author(s): Yoshito Harada, Yuhei Takagaki, Takako Saitoh, Masahiko Sunagawa, Hitoshi Sawada
  • Organizer: 4^<th> Tunicate Meeting Meeting
  • Place of Presentation: Villefranche-sur-Mer(France)
• [Presentation] Sperm GPI-anchored glycoprotein HrUrabin is a binding partner for an allorecognizable egg-coat sperm-receptor HrVC70 during fertilization of the ascidian Halocynthia roretzi (2007)
  • Author(s): Hitoshi Sawada
  • Organizer: 4^<th> Tunicate Meeting Meeting
  • Place of Presentation: Villefranchesur- Mer(France)
• [Book] 受精におけるプロテアソーム「精子学(毛利秀雄・星元紀監修) (2006)
  • Author(s): 澤田均
  • Publisher: 東京大学出版会
• [Book] 新編「精子学」(毛利秀雄、星元紀 監修) (2006)
  • Author(s): 澤田 均 ほか
  • Total Pages: 489
  • Publisher: 東京大学出版会
• [Remarks]
  • URL: http://www.bio.nagoya-u.ac.jp/~SugashimaMBL/Marine.html
• [Remarks]
  • URL: http://www.bio.nagoya-u.ac.jp/~SugashimaMBL/Marine.html
• [Remarks]
  • URL: http://www.bio.nagoya-u.ac.jp/%7eSugashimaMBL/Marine.html