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Analysis of endosome/post-endosome system with special reference to water channel aquaporin 2

Research Project

Project/Area Number 18390057
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionGunma University

Principal Investigator

TAKATA Kuniaki  Gunma University, 学長 (20129290)

Co-Investigator(Kenkyū-buntansha) HAGIWARA Haruo  群馬大学, 大学院・医学系研究科, 准教授 (80189464)
AOKI Takeo  群馬大学, 大学院・医学系研究科, 講師 (70150919)
SUZUKI Takeshi  群馬大学, 大学院・医学系研究科, 助教 (00261868)
MATSUZAKI TOSHIYUKI  日本医科大学, 医学部, 准教授 (30334113)
Project Period (FY) 2006 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥15,950,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2006: ¥6,200,000 (Direct Cost: ¥6,200,000)
Keywordsアクアポリン2 / エンドソーム / 小胞 / トラッフィッキング / 水輸送 / グルコーストランスポーター / トラフィッキング / ゴルジ装置 / チャネル / グルコーストランスポーター4
Research Abstract

Vesicular trafficking that determines the number of cell surface molecules serves as one of important regulatory mechanisms found in the control of the reabsorption of water in the kidney, maintenance of blood glucose level, etc.Intracellular vesicles involved in the endocytosis and subsequent recycling process, such as endosomes and post-endosomal vesicles are key components in such vesicular trafficking and storage. We used water channel aquaporin 2 whose trafficking is regulated by hypophysial hormone vasopressin. Glucose transporter 4(GLUT4), whose trafficking is regulated by insulin in adipocytes and muscle cells, was coexpressed with aquaporin 2 in MDCK cells, and their localization was visualized by immunofluorescence and GFP-tagging methods. Aquaporin 2 and GLUT4 exhibited distinct localization pattern each other in both the resting and stimulated states, showing that aquaporin 2 and GLUT4 are stored in distinct post-endosomal compartments each other, and that their trafficking is differentially regulated. Translocation of aquaporin 2 has been considered to be regulated by its phosphorylation. We raised antibodies that specifically detect phosphorylated aquaporin 2. By western blotting and immunofluorescence microscopy, phosphorylation of aquaporin 2 was found not only on the cell surface but also intracellular vesicles, suggesting the ubiquitous presence of phosphorylated aquaporin 2.

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report   Self-evaluation Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (55 results)

All 2009 2008 2007 2006 Other

All Journal Article (28 results) (of which Peer Reviewed: 22 results) Presentation (20 results) Book (6 results) Remarks (1 results)

  • [Journal Article] Roles of gap junctions in glucose transport from glucose transporter 1-positive to-negative cells in the lateral wall of the rat cholera2009

    • Author(s)
      Suzuki T, Matsunami T, Hisa Y, Takata K, Takamatsu T, Oyamada M
    • Journal Title

      Histochem Cell Biol 131

      Pages: 89-102

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical localization of the aquaporins AQP1, AQP3, AQP4, and AQP5 in the mouse respiratory system2009

    • Author(s)
      Matsuzaki T, Hata H, Ozawa H, Takata K
    • Journal Title

      Acta Histochem Cytochem 42

      Pages: 159-169

    • NAID

      130000115403

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 腎臓と唾液腺における細胞膜水チャネル, アクアポリン2009

    • Author(s)
      松崎利行, 高田邦昭, 小澤一史
    • Journal Title

      顕微鏡 44

      Pages: 111-116

    • NAID

      130007788861

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roles of gap junctions in glucose transport from glucose transporter 1-positive to-negative cells in the lateral wall of the rat cholera.2009

    • Author(s)
      Suzuki T, et al.
    • Journal Title

      Histochem Cell Biol 131

      Pages: 89-102

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical localization of the aquaporins AQP1, AQP3, AQP4, and AQP5 in the mouse respiratory system.2009

    • Author(s)
      Matsuzaki T, et al.
    • Journal Title

      Acta Histochem Cytochem 42

      Pages: 159-169

    • NAID

      130000115403

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 腎臓と唾液腺における細胞膜水チャネル,アクアポリン2009

    • Author(s)
      松崎利行, 他
    • Journal Title

      顕微鏡 44

      Pages: 111-116

    • NAID

      130007788861

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Immunolocalization of water channel aquaporins in the vomeronasal organ of the rat : Expression of AQP4 in neuronal sensory cells2008

    • Author(s)
      Ablimit A, Aoki T, Matsuzaki T, Suzuki T, Hagiwara H, Takami S, Takata K
    • Journal Title

      Chemical Senses 33

      Pages: 481-488

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-term forskolin stimulation induces AMPK activation and thereby enhances tight junction formation in human placental trophoblast BeWo cells2008

    • Author(s)
      Egawa M, Kamata H, Kushiyama A, Sakoda H, Fujishiro M, Horike N, Yoneda M, Nakatsu Y, Ying G, Jun Z, Tsuchiya Y, Takata K, Kurihara H, Asano T
    • Journal Title

      Placenta 29

      Pages: 1003-1008

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Localization and trafficking of aquaporin 2 in the kidney2008

    • Author(s)
      Takata K, Matsuzaki T, Tajika Y, Ablimit A, Hasegawa H
    • Journal Title

      Hisochem Cell Biol 130

      Pages: 187-209

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Localization and trafficking of aquaporin 2 in the kidney2008

    • Author(s)
      Takata K, Matsuzaki T, Tajika Y, Ablimit A, & Hasegawa H
    • Journal Title

      Hisochem Cell Biol 130

      Pages: 187-209

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Immunolocalization of water channel aquaporins in the vomeronasal organ of the rat: Expression of AQP4 in neuronal sensory cells.2008

    • Author(s)
      Ablimit A
    • Journal Title

      Chemical Senses 33

      Pages: 481-488

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-term forskolin stimulation induces AMPK activation and there by enhances tight junction formation in human placehtal trophoblast BeWo cells.2008

    • Author(s)
      Egawa M
    • Journal Title

      Placenta 29

      Pages: 1003-1008

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Localization and trafficking of aquaporin 2 in the kidney.2008

    • Author(s)
      Takata K
    • Journal Title

      Hisochem Cell Biol 130

      Pages: 187-209

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Internalization of caveolae and their relationship with endosomes in cultured human and mouse endothelial cells2007

    • Author(s)
      Aoki T, Hagiwara H, Matsuzaki T, Suzuki T, Takata K
    • Journal Title

      Anat Sci Int 82

      Pages: 82-97

    • NAID

      10019993028

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential localization of aquaporin-2 and glucose transporter 4 in polarized MDCK cells2007

    • Author(s)
      Hasegawa T, Matsuzaki T, Tajika Y, Ablimit A, Suzuki T, Aoki T, Hagiwara H, Takata K
    • Journal Title

      Histochem Cell Biol 127(3)

      Pages: 233-241

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Internalization of caveolae and their relationship with endosomes in cultured human and mouse endothelial cells2007

    • Author(s)
      Aoki T, Hagiwara H, Matsuzaki T, Suzuki T & Takata K
    • Journal Title

      Anat Sci Int 82

      Pages: 82-97

    • NAID

      10019993028

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Differential localization of aquaporin-2 and glucose transporter 4 in polarized MDCK cells2007

    • Author(s)
      Hasegawa T, Matsuzaki T, Tajika Y, Ablimit A, Suzuki T, Aoki T, Hagiwara H & Takata K
    • Journal Title

      Histochem Cell Biol 127(3)

      Pages: 233-241

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Differential localization of aquaporin-2 and glucose transporter4in polarized MDCK cells2007

    • Author(s)
      Hasegawa T
    • Journal Title

      Histochem Cell Biol 127

      Pages: 233-241

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] lnternalization of caveolae and their relationship with endosomes in cultured human and mouse endothelial cells2007

    • Author(s)
      Aoki T
    • Journal Title

      Anat Sci Int 82

      Pages: 82-97

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunolocalization of water channel aquaporins in the nasal olfactory mucosa2006

    • Author(s)
      Ablimit A, Matsuzaki T, Tajika Y, Aoki T, Hagiwara H, Takata K
    • Journal Title

      Arch Histol Cytol 69

      Pages: 1-12

    • NAID

      10018116835

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aquaporin-2 (AQP2) : Its intracellular compartment and trafficking2006

    • Author(s)
      Takata K
    • Journal Title

      Cell Mol Biol 52

      Pages: 34-39

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunolocalization of water channel aquaporins in the nasal olfactory mucosa2006

    • Author(s)
      Ablimit A, Matsuzaki T, Tajika Y, Aoki T, Hagiwara H & Takata K
    • Journal Title

      Arch Histol Cytol 69

      Pages: 1-12

    • NAID

      10018116835

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Aquaporin-2(AQP2) : Its intracellular compartment and trafficking2006

    • Author(s)
      Takata K
    • Journal Title

      Cell Mol Biol 52

      Pages: 34-39

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Immunolocalization of water channel aquaporins in the nasal olfactory mucosa.2006

    • Author(s)
      Ablimit A
    • Journal Title

      Arch Histol Cytol 69

      Pages: 1-12

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Gap junctions mediate glucose transport between GLUT1-positive and -negative cells in the rat cochlea.2006

    • Author(s)
      Suzuki T
    • Journal Title

      Cell Commun Adhesion 13

      Pages: 93-102

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Changes of aquaporin 5-distribution during release and reaccumulation of secretory granules in isoproterenol-treated mouse parotid gland.2006

    • Author(s)
      Matsuzaki T
    • Journal Title

      J Electron Microsc 55

      Pages: 183-189

    • NAID

      10018791161

    • Related Report
      2006 Annual Research Report
  • [Journal Article] デコンボリューション顕微鏡法2006

    • Author(s)
      鈴木健史, 高田邦昭
    • Journal Title

      顕微鏡 41

      Pages: 120-123

    • NAID

      10018183215

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Aquaponn-2 (AQP2) its intracellular compartment and trafficking.2006

    • Author(s)
      Takata K
    • Journal Title

      Cell Mol Biol 52

      Pages: 34-39

    • Related Report
      2006 Annual Research Report
  • [Presentation] Immunohistochemical distribution of water channel, AQP4, in the rat central nervous system2009

    • Author(s)
      Matsuzaki T, Sawai N, Takata K, Ozawa H
    • Organizer
      The 8th World Congress on Neurohypophysial Hormones
    • Place of Presentation
      Kitakyushu, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] MDCK細胞における頂部細胞膜カベオラと水チャネルアクアポリン2との関係2009

    • Author(s)
      青木武生, 萩原治夫, 鈴木健史, 高田邦昭
    • Organizer
      日本組織細胞化学会50回総会学術集会
    • Place of Presentation
      大津
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] マウス呼吸器系における水チャネル、アクアポリンの分布局在2009

    • Author(s)
      松崎利行, 畑英一, 高田邦昭, 小澤一史
    • Organizer
      日本組織細胞化学会50回総会学術集会
    • Place of Presentation
      大津
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Immunohistochemical distribution of water channel, AQP4, in the rat central nervous system.2009

    • Author(s)
      松崎利行, et al.
    • Organizer
      The 8th World Congress on Neurohypophysial Hormones
    • Place of Presentation
      Kitakyushu, Japan
    • Related Report
      2009 Annual Research Report
  • [Presentation] Localization and trafficking of aquaporin 2 in the kidney2008

    • Author(s)
      Takata K
    • Organizer
      50th Symposium of the Society for Histochemistry (Rober Feulgen Lecture 2008)
    • Place of Presentation
      Interlaken, Switzerland
    • Related Report
      2009 Final Research Report
  • [Presentation] Intracellular localization and trafficking of water channel aquaporin 2 (AQP2)2008

    • Author(s)
      Takata K
    • Organizer
      9th Asia-Pacific Microscopy Conference, Jeju (Symposium L-07 Recent Progress of Functional Morphology of the Kidney)
    • Place of Presentation
      Korea
    • Related Report
      2009 Final Research Report
  • [Presentation] Localization and trafficking of aquaporin 2 in the kidney2008

    • Author(s)
      Takata K
    • Organizer
      50th Symposium of the Society for Histochemistry(Rober Feulgen Lecture 2008)
    • Place of Presentation
      Interlaken, Switzerland
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] Intracellular localization and trafficking of water channel aquaporin 2(AQP2). 9th Asia-Pacific Microscopy Conference2008

    • Author(s)
      Takata K
    • Organizer
      Jeju(Symposium L-07 Recent Progress of Functional Morphology of the Kidney)
    • Place of Presentation
      Korea
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] Localization and trafficking of aquaporin 2 in the kidney.2008

    • Author(s)
      Takata K
    • Organizer
      50th Symposium of the Society for Histochemistry (Rober Feulgen Lecture 2008)
    • Place of Presentation
      lnterlaken, Switzerland
    • Related Report
      2008 Annual Research Report
  • [Presentation] Intracellular localization and trafficking of water channel aquaporin 2 (AQP2).2008

    • Author(s)
      Takata K
    • Organizer
      9th Asia-Pacific Microscopy Conference
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2008 Annual Research Report
  • [Presentation] 免疫シナプスにおけるPKC分子動態制御機購2008

    • Author(s)
      鈴木 健史
    • Organizer
      第113回日本解剖学会総会・全国学術集会
    • Place of Presentation
      大分
    • Related Report
      2007 Annual Research Report
  • [Presentation] Intracelluar compartment of aquaporin-2 (AQP2) and glucose transporter-4 (GLUT4) in MDCK Cells2007

    • Author(s)
      Takata K, Hasegawa T, Matsuzaki T, Tajika Y, Ablimit A, Suzuki T, Aoki T, Hagiwara H
    • Organizer
      The 5th International Conference of Aquaporin
    • Place of Presentation
      Nara, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] Intracelluar compartment of aquaporin-2(AQP2) and glucose transporter-4(GLUT4) in MDCK Cells2007

    • Author(s)
      Takata K, Hasegawa T, Matsuzaki T, Tajika Y, Ablimit A, Suzuki T, Aoki T & Hagiwara H
    • Organizer
      The 5th International Conference of Aquaporin
    • Place of Presentation
      Nara, Japan
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] 免疫シナプスにおける分子分布動態の3次元ライブセルイメージング解析2007

    • Author(s)
      鈴木 健史
    • Organizer
      日本顕微鏡学会・第63回学術講演会
    • Place of Presentation
      新潟
    • Related Report
      2007 Annual Research Report
  • [Presentation] Aquaporin-2(AQP2) is endocytosed via caveohn-dependent,clathrin-indenendent pathway in MDCK cells2007

    • Author(s)
      Aoki T
    • Organizer
      The 5th International Conference of Aquaporin
    • Place of Presentation
      Nara,Japan
    • Related Report
      2007 Annual Research Report
  • [Presentation] Ihtracelluar compartment of aquaporin-2(AQP2) and glucose transnorter-4(GLUT4) in MDCK Cells2007

    • Author(s)
      Takata K
    • Organizer
      The 5th International Conference of Aquaporin
    • Place of Presentation
      Nara,Japan
    • Related Report
      2007 Annual Research Report
  • [Presentation] 免疫シナプス超分子クラスターの分子機能動態.(シンポジウム2バイオイメージングで生体機能を観る、測る)2007

    • Author(s)
      鈴木 健史
    • Organizer
      第48回日本組織細胞化学会総会、第39回日本臨床分子形態学会総会・合同学術集会
    • Place of Presentation
      甲府
    • Related Report
      2007 Annual Research Report
  • [Presentation] 生命科学における顕微鏡-形を見る意味-2007

    • Author(s)
      高田 邦昭
    • Organizer
      日本顕微鏡学会第51回シンポジウム
    • Place of Presentation
      徳島
    • Related Report
      2007 Annual Research Report
  • [Presentation] Intracellular compartment and trafficking of aquaporin-2 water channel2006

    • Author(s)
      Takata K, Tajika Y, Matsuzaki T, Suzuki T, Aoki T, Hagiwara H
    • Organizer
      The16th International Microscopy Congress
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2009 Final Research Report 2008 Self-evaluation Report
  • [Presentation] Internalization of caveolae and their relationship with endosomes2006

    • Author(s)
      Aoki T, Suzuki T, Matsuzaki T, Hagiwara H, Takata K
    • Organizer
      The16th International Microscopy Congress
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2009 Final Research Report 2008 Self-evaluation Report
  • [Book] アクアポリンの分布 (動物)。からだと水の事典 (佐々木成, 石橋賢一編)2008

    • Author(s)
      高田邦昭, 松崎利行
    • Publisher
      朝倉書店
    • Related Report
      2009 Final Research Report
  • [Book] アクアポリンの分布(動物)からだと水の事典(佐々木成, 石橋賢一)2008

    • Author(s)
      高田邦昭, 松崎利行
    • Publisher
      朝倉書店(東京)
    • Related Report
      2008 Self-evaluation Report
  • [Book] からだと水の事典(佐々木成、石橋賢一編)2008

    • Author(s)
      高田邦昭
    • Total Pages
      375
    • Publisher
      朝倉書店、東京
    • Related Report
      2008 Annual Research Report
  • [Book] 染色・バイオイメージング実験ハンドブック。細胞や組織の形態・遺伝子・タンパク質を観るための染色法と顕微鏡観察のすべて。実験医学別冊2006

    • Author(s)
      高田邦昭, 斎藤尚亮, 川上速人, 編
    • Publisher
      羊土社
    • Related Report
      2009 Final Research Report
  • [Book] 染色・バイオイメージング実験ハンドブック。細胞や組織の形態・遺伝子・タンパク質を観るための染色法と顕微鏡観察のすべて。実験医学別冊(高田邦昭, 斎藤尚亮, 川上速人)2006

    • Publisher
      羊土社(東京)
    • Related Report
      2008 Self-evaluation Report
  • [Book] 染色・バイオイメージング実験ハンドブック2006

    • Author(s)
      青木武生
    • Publisher
      羊土社
    • Related Report
      2006 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.med.gunma-u.ac.jp/anatcellbiol/

    • Related Report
      2009 Final Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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