Molecular analysis of epigenetic factors involved in the induction of spermatogenic cell apoptosis
| Project/Area Number |
18390060
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Nagasaki University |
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Principal Investigator |
KOJI Takehiko Nagasaki University, 大学院・医歯薬学総合研究科, 教授 (30170179)
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| Co-Investigator(Kenkyū-buntansha) |
HISHIKAWA Yoshitaka 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (60304276)
IZUMI Shinichi 長崎大学, 大学院・医歯薬学総合研究科, 助教 (40264246)
AN Shucai 長崎大学, 大学院・医歯薬学総合研究科, 助教 (30404213)
SATO Yoko 長崎大学, 大学院・医歯薬学総合研究科, 助教 (50398963)
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| Project Period (FY) |
2006 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥16,480,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2007: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2006: ¥7,900,000 (Direct Cost: ¥7,900,000)
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| Keywords | 精子形成細胞 / DNAのメチル化 / 分子組織化学 / ヒストン蛋白修飾 / 免疫組織化学 / ヘテロクロマチン化 / アポトーシス / 初代培養 |
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| Research Abstract |
雄性生殖細胞を生み出す精子形成過程では、精子換算で約25-75%の生殖細胞が失われることに注目し、この自発的細胞死の誘導機構を解析した。特に本過程がDNAからなるクロマチンの規則的な凝集過程であり、その調節破綻が自殺死誘導の原因ではないかと考え、クロマチン凝集に深く関与するDNAのメチル化を独自に開発したHELMET法により解析し、死を誘導された細胞核では顕著にDNAの非メチル化が生じていることを初めて明らかにした。
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Report
(4 results)
Research Products
(68 results)
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[Journal Article] Deubiquitylation of histone H2A activates transcriptional initiation viatrans-histone cross-talk with H3K4 di-and trimethylation2008
Author(s)
Nakagawa T, Kajitani T, Togo S, Masuko N, Ohdan H, Hishikawa Y, Koji T, Matsuyama T, Ikura T, Muramatsu M, Ito T
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Journal Title
Genes Dev 22
Pages: 37-49
Related Report
Peer Reviewed
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[Journal Article] Renoprotective effect of azelnidipine in rats2008
Author(s)
Kurashige T, Abe K, Furusu A, Miyazaki M, Obata Y, Xia Z, Nakazawa M, Nakazawa Y, Funakoshi S, Harada T, Koji T, Kohno S
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Journal Title
Biol Pharm Bull 31
Pages: 2237-2244
NAID
Related Report
Peer Reviewed
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[Journal Article] Adhesion-dependent growth of primary adult T cell leukemia cells with down-regulation of HTLV-I p40Tax protein : a novel in vitro model of the growth of acute ATL cells2008
Author(s)
Nagai K, Jinnai I, Hata T, Usui T, Sasaki D, Tsukasaki K, Sugahara K, Hishikawa Y, Yamada Y, Tanaka Y, Koji T, Mano H, Kamihira S, Tomonaga M
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Journal Title
Int J Hematol 88
Pages: 551-564
NAID
Related Report
Peer Reviewed
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[Journal Article] Hyperthyroidism induces apoptosis in rat liver through activation of death receptor-mediated pathways2007
Author(s)
Kumar A, Sinha RA, Tiwari M, Singh R, Koji T, Manhas N, Rastogi L, Pal L, Shrivastava A, Sahu RP, Godbole MM
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Journal Title
J Hepatol 46
Pages: 888-898
Related Report
Peer Reviewed
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[Journal Article] A novel alternative splicing isoform of human T-cell leukemia virus type 1 bZIP factor(HBZ-SI)targets distinct subnuclear localization2006
Author(s)
Murata K, Hayashibara T, Sugahara K, Uemura A, Yamaguchi T, Harasawa H, Hasegawa H, Tsuruda K, Okazaki T, Koji T, Miyanishi T, Yamada Y, Kamihira S
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Journal Title
J Virol 80
Pages: 2495-2505
Related Report
Peer Reviewed
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