Molecular Physiological Mechanism of Chloride Ion Secretion by CLC-5 and KCC4 associated with Gastric Proton Pump

• Project/Area Number: 18390064
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: University of Toyama
• Principal Investigator: SAKAI Hideki University of Toyama, 大学院・医学薬学研究部(薬学), 教授 (60242509)
• Co-Investigator(Kenkyū-buntansha): MORII Magotoshi 鈴鹿医療科学大学, 薬学部, 教授 (60019130) ; TAKAHASHI Yuji 富山大学, 大学院・医学薬学研究部(薬学), 助教 (30422629)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥8,480,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2006: ¥3,800,000 (Direct Cost: ¥3,800,000)
• Keywords: イオン輸送 / 蛋白質 / 胃酸分泌 / プロトンポンプ / 塩化物イオン / 胃酸分泌細胞 / 塩素イオン / トランスポーター / CLC-5 / KCC4 / ベシクル / 細管小胞 / 胃腺 / H^+,K^+-ATPase / K^+-Cl^-共輸送体 / Cl^-チャネル / 能動輸送

Research Abstract

胃酸(塩酸 : HCl)のプロトン(H^+)を輸送する蛋白質が、胃プロトンポンプ(H^+,K^+-ATPase)であることはよく知られているが、塩化物(塩素)イオン(Cl^-)を輸送する蛋白質については、これまで確定していなかった。本研究で、Cl-輸送蛋白質のKCC4とCLC-5が胃酸分泌機構に関与することを見出した。KCC4は胃酸分泌細胞のアピカル膜で、胃プロトンポンプと共に、空腹時(胃酸分泌休止時)の胃酸分泌に主として関与し、CLC-5は細管小胞膜上で、プロトンポンプと共に、摂食時(胃酸分泌刺激時)の胃酸分泌に関与することがわかり、空腹時と摂食時で異なる胃酸分泌機構が機能していることが明らかとなった。

Research Products

• [Journal Article] Functional association between K^+-Cl^- cotransporter-4 and H^+,K^+-ATPase in the apical canalicular membrane of gastric parietal cells (2009)
  • Author(s): Fujii T, Takahashi Y, Ikari A, Morii M, Tabuchi Y, Tsukada K, Takeguchi N, Sakai H.
  • Journal Title: J. Biol. Chem 284 Pages: 619-629
  • Peer Reviewed
• [Journal Article] Involvement of aquaporin-5 in differentiation of human gastric cancer cells (2009)
  • Author(s): Watanabe T, Fujii T, Oya T, Horikawa N, Tabuchi Y, Takahashi Y, Morii M, Takeguchi N, Tsukada K, Sakai H.
  • Journal Title: J. Physiol. Sci 59 Pages: 113-122
  • NAID: 10025218436
  • Peer Reviewed
• [Journal Article] determination of the absolute stereochemistry, and evaluations at the nicotinic acetylcholine receptors of a hydroxyindolizidine alkaloid from the ant Myrmicaria melanogaster (2009)
  • Author(s): Zhou D, Sakai H, et al. (14名, 8番目) Synthesis
  • Journal Title: Heterocycles 79 Pages: 565-571
  • Peer Reviewed
• [Journal Article] Functional association between K^+-Cl^- cotransporter-4 and H^+, K^+-ATPase in the apical canalicular membrane of gastric parietal cells. (2009)
  • Author(s): Fujii T., et al.
  • Journal Title: J. Biol. Chem. 284 Pages: 619-629
  • Peer Reviewed
• [Journal Article] Involvement of aquaporin-5 in differentiation of human gastric cancer cells. (2009)
  • Author(s): Watanabe T., et al.
  • Journal Title: J. Physiol. Sci. 59 Pages: 113-122
  • Peer Reviewed
• [Journal Article] K^+-Cl^- cotransporter-3a up-regulates Na^+,K^+-ATPase in lipid rafts of gastric luminal parietal cells (2008)
  • Author(s): Fujii T, Takahashi Y, Itomi Y, Fujita K, Morii M, Tabuchi Y, Asano S, Tsukada K, Takeguchi N, Sakai H.
  • Journal Title: J. Biol. Chem 283 Pages: 6869-6877
  • NAID: 120002226796
  • Peer Reviewed
• [Journal Article] Involvement of the H_3O^+-Lys-164-Gln-161-Glu-345 charge transfer pathway in proton transport of gastric H^+, K^+-ATPase (2008)
  • Author(s): Morii M, Yamauchi M, Ichikawa T, Fujii T, Takahashi Y, Asano S, Takeguchi N, Sakai H.
  • Journal Title: J. Biol. Chem 283 Pages: 16876-16884
  • Peer Reviewed
• [Journal Article] Functional consequences of various leucine mutations in the M3/M4 loop of the Na^+,K^+-ATPase α-subunit (2008)
  • Author(s): Eguchi H, Morii M, Takahashi Y, Sakai H, Nakano M, Ochiai H, Shirahata A, Hara Y, Kawamura M, Takeda K.
  • Journal Title: J. Membr. Biol 221 Pages: 133-140
  • Peer Reviewed
• [Journal Article] Claudin-16 is directly phosphorylated by protein kinase A independently of a vasodilator- stimulated phosphor-protein mediated pathway (2008)
  • Author(s): Ikari A, Sakai H, et.al. (10人, 7番目)
  • Journal Title: J.Cell.Physiol 214 Pages: 221-229
  • Peer Reviewed
• [Journal Article] Syntheses of the proposed structures of poison-frog alkaloids 179 and 207E and their inhibitory effects on neuronal nicotinic acetylcholine receptors (2008)
  • Author(s): Toyooka N, Sakai H, et al. (14人中6番目)
  • Journal Title: Synlett 1 Pages: 61-64
  • Peer Reviewed
• [Journal Article] Involvement of the H_3O^+-Lys-164-Gln-161-Glu-345 charge transfer pathway in proton transport of gastric H^+, K^+-ATPase. (2008)
  • Author(s): Morii M., et al.
  • Journal Title: J. Biol. Chem. 283 Pages: 16876-16884
  • Peer Reviewed
• [Journal Article] Involvement of KCC4 in the apical K^+ recycling mechanism of gastric parietal cells. (2008)
  • Author(s): Fujii T., et al.
  • Journal Title: J. Pharm. Soc. Jpn. 128 Pages: 19-20
• [Journal Article] Functional relation between Cl^-/H^+ antiporter CLC-5 and gastric protonpump. (2008)
  • Author(s): Furuya H., et al.
  • Journal Title: J. Pharm. Soc. Jpn. 128 Pages: 21-22
• [Journal Article] Claudin-16 is directly phosphorylated by protein kinase A independently of avasodilator-stimulated phosphoproteiirmediated pathway (2008)
  • Author(s): Ikari A., et. al.
  • Journal Title: J. CeU. Physiol 214 Pages: 221-229
  • Peer Reviewed
• [Journal Article] Syntheses of the Droposed structures of poison-frog alkaloids 179 and 207E and their inhibitory effects on neuronal nicotinic acetvlcholine recentors (2008)
  • Author(s): Toyooka N., et. al.
  • Journal Title: Synlett 1 Pages: 61-64
  • Peer Reviewed
• [Journal Article] Functional consequences of various leucine mutations in the M3/M4 loop of the Na^+,K^+-ATPase crsubunit (2008)
  • Author(s): Eguchi H., et. al.
  • Journal Title: J. Membr. Biol 221 Pages: 133-140
  • Peer Reviewed
• [Journal Article] K^+-CI- cotransporter- 3a up-regulates Na^+,K^+-ATPase in lipid rafts of gastric luminal parietal cells (2008)
  • Author(s): Fujii T., et. al.
  • Journal Title: J. Biol. Chem 283 Pages: 6869-6877
  • Peer Reviewed
• [Journal Article] Inhibition of P-type ATPases by [(dihydroindenyl)oxy]acetic acid (DIOA), a K^+-Cl^- cotransporter inhibitor (2007)
  • Author(s): Fujii T, Ohira Y, Itomi Y, Takahashi Y, Asano S, Morii M, Takeguchi N, Sakai H.
  • Journal Title: Eur. J. Pharmacol 560 Pages: 123-126
  • Peer Reviewed
• [Journal Article] L-type amino acid transporter-1 expressed in human astrocytomas, U343MGa (2007)
  • Author(s): Asano S, Sakai H, et al. (9名, 5番目)
  • Journal Title: Biol. Pharm. Bull 30 Pages: 415-422
  • NAID: 110006239177
  • Peer Reviewed
• [Journal Article] Synthesis of poison-frog alkaloids 233A, 235U, and 251AA and their inhibitory effects on neuronal nicotinic acetylcholine receptors (2007)
  • Author(s): Toyooka N, Sakai H, et al. (11名, 6番目)
  • Journal Title: Bioorg. Med. Chem. Lett 17 Pages: 5872-5875
  • Peer Reviewed
• [Journal Article] Flexible synthesis of poison-frog alkaloids of the 5,8 disubstituted indolizidine-class. II : Synthesis of (-)-209B, (-)-231C, (-)-233D, (-) 235B'', (-)-221I, and an epimer of 193E and pharmacological effects at neuronal nicotinic acetylcholine receptors (2007)
  • Author(s): Kobayashi S, Sakai H, et al. (11名, 7番目)
  • Journal Title: Beilstein J. Org. Chem 3 Pages: 30-30
  • Peer Reviewed
• [Journal Article] Synthesis of poison-frog alkaloids 233A, 235U, and 251AA and their inhibitory effects on neuronal nicotinic acetvlcholine recentors (2007)
  • Author(s): Toyooka N., et. al.
  • Journal Title: Bioorg. Med. Chem. Lett 17 Pages: 5872-5875
  • Peer Reviewed
• [Journal Article] Flexible synthesis of poison-frog alkaloids of the 5,8-disubstituted indolizidine-class.II: Synthesis of(-)-209B, (-)-231C, (-)-233D, (-)-235B", (-)-221I, and an enimer of l93E and pharmacological effects at neuronal nicotinic acetylcholine receptors (2007)
  • Author(s): Kobayashi S., et. al.
  • Journal Title: Beilstein J. Org. Chem 3 Pages: 30-30
  • Peer Reviewed
• [Journal Article] 胃酸分泌細胞の塩素イオン分泌機構に関与するイオンチャネルとトランスポーター (2007)
  • Author(s): 酒井 秀紀
  • Journal Title: G. I. Research 15 Pages: 111-115
• [Journal Article] L-type amino acid transporter-1 expressed in human astrocytomas, U343MGa (2007)
  • Author(s): Asano S., et al.
  • Journal Title: Biol.Pharm.Bull. 30巻・3号 Pages: 415-422
• [Journal Article] Inhibition of P-type ATPases by [(dihydroindenyl)oxy]acetic acid (DIOA), a K^+-Cl^-cotransporter inhibitor. (2007)
  • Author(s): Fujii T., et al.
  • Journal Title: Eur.J.Pharmacol. 560巻・2-3号 Pages: 123-126
• [Journal Article] Upregulation of thromboxane synthase in human colorectal carcinoma and the cancer cell proliferation by thromboxane A2 (2006)
  • Author(s): Sakai H, Suzuki T, Takahashi Y, Ukai M, Tauchi K, Fujii T, Horikawa N, Minamimura T, Tabuchi Y, Morii M, Tsukada K, Takeguchi N.
  • Journal Title: FEBS Lett 580 Pages: 3368-3374
  • Peer Reviewed
• [Journal Article] Overexpression of Hsp70 partially restores the structural stability and functional defects of temperature- sensitive mutant of large T-antigen at nonpermissive temperature (2006)
  • Author(s): Tabuchi Y, Sakai H, et al. (8名, 5番目)
  • Journal Title: Cell Stress Chaperones 11 Pages: 259-267
  • Peer Reviewed
• [Journal Article] Genetic networks responsive to sodium butyrate in colonic epithelial cells (2006)
  • Author(s): Tabuchi Y, Sakai H, et al.(6名, 5番目)
  • Journal Title: FEBS Lett 580 Pages: 3035-3041
  • Peer Reviewed
• [Journal Article] Upregulation of thromboxane synthase in human colorectal carcinoma and the cancer cell proliferation by thromboxane A_2. (2006)
  • Author(s): Sakai H., et al.
  • Journal Title: FEBS Lett. 580巻・14号 Pages: 3368-3374
• [Journal Article] Overexpression of Hsp70 partially restores the structural stability and functional defects of temperature-sensitive mutant of large T-antigen at nonpermissive temperature. (2006)
  • Author(s): Tabuchi Y., et al.
  • Journal Title: Cell Stress Chaperones 11巻・3号 Pages: 259-267
• [Journal Article] Genetic networks responsive to sodium butyrate in colonic epithelial cells. (2006)
  • Author(s): Tabuchi Y., et al.
  • Journal Title: FEBS Lett. 580巻・13号 Pages: 3035-3041
• [Presentation] 胃プロトンポンプ阻害剤オメプラゾールとSCH28080は結合ポケットを共有する (2009)
  • Author(s): 森井孫俊, 酒井秀紀
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2009-03-27
• [Presentation] 胃壁細胞アピカル膜の酸分泌機構の分子生理 (2009)
  • Author(s): 藤井拓人, 酒井秀紀
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2009-03-27
• [Presentation] 酸分泌休止時と刺激時における胃プロトンポンプの性質 (2009)
  • Author(s): 藤田恭輔, 酒井秀紀
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2009-03-26
• [Presentation] 胃酸分泌細胞の細管小胞膜とアピカル膜におけるプロトンポンプの諸性質 (2009)
  • Author(s): 藤田 恭輔
  • Organizer: フィジカルファーマフォーラム2009
  • Place of Presentation: 大阪府高槻市
  • Year and Date: 2009-03-24
• [Presentation] 胃酸分泌細胞におけるK^+-Cl^-共輸送体KCC4の機能 (2008)
  • Author(s): 藤井 拓人
  • Organizer: トランスポーター研究会ワークショップin鶴岡
  • Place of Presentation: 山形県鶴岡市
  • Year and Date: 2008-11-16
• [Presentation] KCC3aによるNa^+, K^+-ATPase活性調節機構におけるコレステロールの機能 (2008)
  • Author(s): 藤田 恭輔
  • Organizer: 日本薬学会北陸支部第119回例会
  • Place of Presentation: 石川県金沢市
  • Year and Date: 2008-11-09
• [Presentation] 胃プロトンポンプ活性調節におけるコレステロールの役割 (2008)
  • Author(s): 岡崎 真也
  • Organizer: 日本薬学会北陸支部第119回例会
  • Place of Presentation: 石川県金沢市
  • Year and Date: 2008-11-09
• [Presentation] 胃プロトンポンプの活性調節における膜マイクロドメインの役割 (2008)
  • Author(s): 酒井秀紀
  • Organizer: 第30回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2008-08-07
• [Presentation] CLC-5 Cl^-/H^+交換輸送体と胃プロトンポンプとの機能連関 (2008)
  • Author(s): 降矢裕史, 酒井秀紀
  • Organizer: 第30回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2008-08-07
• [Presentation] KCC4が関与する胃酸分泌細胞アピカル膜のK^+リサイクリング機構 (2008)
  • Author(s): 藤井拓人, 酒井秀紀
  • Organizer: 第30回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2008-08-07
• [Presentation] 胃酸分泌細胞の膜マイクロドメインの構成と機能 (2008)
  • Author(s): 酒井 秀紀
  • Organizer: 自然科学研究機構生理学研究所研究会
  • Place of Presentation: 愛知県岡崎市
  • Year and Date: 2008-07-16
• [Presentation] 胃酸分泌細胞の脂質ラフトにおけるK-Cl共輸送体KCC3aとNa^+, K^+-ATPaseの機能連関 (2008)
  • Author(s): 藤井 拓人
  • Organizer: 第3回トランスポーター研究会年会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2008-06-08
• [Presentation] カベオソームとしての胃細管小胞におけるプロトンポンプ活性調節 (2008)
  • Author(s): 高橋佑司, 酒井秀紀
  • Organizer: 第3回トランスポーター研究会年会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2008-06-07
• [Presentation] 胃プロトンポンプ電荷異動路の分子動力学シミュレーション (2008)
  • Author(s): 森井孫俊, 酒井秀紀
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2008-03-28
• [Presentation] ヒト高分化型胃がん細胞におけるアクアポリン-5の極性維持機能 (2008)
  • Author(s): 酒井 秀紀
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2008-03-28
• [Presentation] 胃プロトンポンプ電荷移動路の分子動力学シミュレーション (2008)
  • Author(s): 森井 孫俊
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2008-03-28
• [Presentation] Distribution and function of gastric proton pump in tubulovesicle and apical surface membrane of gastric parietal cells (2008)
  • Author(s): Sakai H
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2008-03-25
• [Presentation] Functional association of K^+-Cl^- cotransporter 4 with gastric H^+,K^+-ATPase (2008)
  • Author(s): Fujii T, Sakai H
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2008-03-25
• [Presentation] Distribution and function of gastric proton pump in tubulovesicle and apical surface membrane of parietal cells. (2008)
  • Author(s): 酒井 秀紀
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2008-03-25
• [Presentation] Functional association of K^+-C1- cotransporter 4 with grastric H^+,K^+-ATPase. (2008)
  • Author(s): 藤井 拓人
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2008-03-25
• [Presentation] Function of extracellular M3/M4 loop of human non-grastric H^+,K^+-←-ATPase, ATP1AL1. (2008)
  • Author(s): 高橋 佑司
  • Organizer: 第85目日本生理学会大会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2008-03-25
• [Presentation] Modulation of Gastric Proton Pump Activity by CLC-5 (2008)
  • Author(s): Takahashi Y, Fujii T, Furuya H, Tabuchi Y, Ikari A, Sakamoto H, Naito I, Manabe K, Uchida S, Sasaki S, Asano S, Morii M, Takeguchi N, Sakai H
  • Organizer: Pan-Pacific International Partnership Conference on Pharmaceutical and Life Sciences (The 4th US-Japan Joint Conference)
  • Place of Presentation: Nagoya
  • Year and Date: 2008-02-22
• [Presentation] Modulation of eastric proton pump activity by CLC-5 (2008)
  • Author(s): 高橋 佑司
  • Organizer: Pan-Pacific International Partnership Conference on Pharmaceutical and Life Sciences
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2008-02-22
• [Presentation] Involvement of the H_3O^+- Lys-164-Gln-161-Glu-345 charge transfer pathway in proton transport of gastric H^+,K^+-ATPase (2008)
  • Author(s): Morii M, Yamauchi M, Ichikawa T, Fujii T, Takahashi Y, Asano S, Takeguchi N, Sakai H
  • Organizer: 12th International ATPase Conference Na, K-ATPase and Related Transport ATPases of P-type : Structures, Mechanisms, and Roles in Health and Disease
  • Place of Presentation: Aarhus, Denmark
• [Presentation] 胃H^+,K^+-ATPaseのプロトン輸送路への変異導入 (2007)
  • Author(s): 山内理嗣, 酒井秀紀
  • Organizer: 第29回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2007-11-27
• [Presentation] K^+-C^l-共輸送体KCC4と胃プロトンポンプの機能連関 (2007)
  • Author(s): 藤井拓人, 酒井秀紀
  • Organizer: 第29回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2007-11-27
• [Presentation] K^+-CI-共輸送体KCC4と胃プロトンポンプの機能連関 (2007)
  • Author(s): 藤井 拓人
  • Organizer: 第29目生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2007-11-27
• [Presentation] がん細胞におけるポンプ、チャネル研究の新展開 (2007)
  • Author(s): 酒井 秀紀
  • Organizer: 第29回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2007-11-26
• [Presentation] KCC4とCLC-5の関与する胃酸分泌機構の解明 (2007)
  • Author(s): 藤井 拓人
  • Organizer: 日本薬学会北陸支部第117回例会
  • Place of Presentation: 石川県金沢市
  • Year and Date: 2007-11-11
• [Presentation] 2つの異なる胃酸分泌最終段階における胃プロトンポンプ複合体 (2007)
  • Author(s): 酒井 秀紀
  • Organizer: 大学共同利用機関法人自然科学研究機構研究会
  • Place of Presentation: 愛知県岡崎市
  • Year and Date: 2007-11-02
• [Presentation] 胃酸分泌刺激に伴う細管小胞膜の胃プロトンポンプ活性変化のメカニズム (2007)
  • Author(s): 高橋佑司, 酒井秀紀
  • Organizer: 第54回中部日本生理学会
  • Place of Presentation: 三重県津市
  • Year and Date: 2007-10-20
• [Presentation] ヒト胃ガン細胞におけるアクアポリン・5の生理機能 (2007)
  • Author(s): 酒井 秀紀
  • Organizer: 第54回中部日本生理学会
  • Place of Presentation: 三重県津市
  • Year and Date: 2007-10-19
• [Presentation] 胃のイオンチャネル、トランスポーターの新規生理機能と創薬への可能性 (2007)
  • Author(s): 酒井 秀紀
  • Organizer: フオーラム富山「創薬」第23回研究会
  • Place of Presentation: 富山県富山市
  • Year and Date: 2007-09-21
• [Presentation] 胃酸分泌細胞におけるK-Cl共輸送体の発現と生理機能 (2007)
  • Author(s): 藤井拓人, 酒井秀紀
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山県富山市
  • Year and Date: 2007-03-29
• [Presentation] 胃プロトンポンプにおける電荷移動と水輸送のカップリング (2007)
  • Author(s): 森井孫俊, 酒井秀紀
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山県富山市
  • Year and Date: 2007-03-29
• [Presentation] 胃プロトンポンプ及びナトリウムポンプに対するCLC-5の機能 (2007)
  • Author(s): 高橋佑司, 酒井秀紀
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山県富山市
  • Year and Date: 2007-03-28
• [Presentation] Function of K-Cl cotransporters in gastric parietal cells (2007)
  • Author(s): Sakai H
  • Organizer: 第84回日本生理学会大会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2007-03-20
• [Presentation] 胃プロトンポンプの分子動力学シミュレーション (2006)
  • Author(s): 森井孫俊, 酒井秀紀
  • Organizer: 第28回生体膜と薬物との相互作用シンポジウム
  • Place of Presentation: 第28回生体膜と薬物との相互作用シンポジウム
  • Year and Date: 2006-11-09
• [Presentation] K-Cl共輸送体によるNa,K-ATPase活性の調節機構 (2006)
  • Author(s): 糸見安生, 酒井秀紀
  • Organizer: 第28回生体膜と薬物との相互作用シンポジウム
  • Place of Presentation: 静岡県静岡市
  • Year and Date: 2006-11-09
• [Presentation] Lumenal gating mechanism of gastric H^+,K^+-ATPase (2006)
  • Author(s): Morii M, Sakai H
  • Organizer: 20^<th> IUBMB International Congress of Biochemistry and Molecular Biology and 11^<th> FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-19
• [Presentation] Regulation of the gastric H,K-ATPase activity by CLC-5 (2006)
  • Author(s): Sakai H
  • Organizer: Molecular Mechanism and Regulation in Cation Transport ATPases and Related Genetic Diseases
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
• [Presentation] Proton pathway of the gastric H^+,K^+-ATPase (2006)
  • Author(s): Morii M, Sakai H
  • Organizer: Molecular Mechanism and Regulation in Cation Transport ATPases and Related Genetic Diseases
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-16
• [Book] 「疾病と病態生理」改訂第2版 (2006)
  • Author(s): 酒井秀紀(橋本隆男, 佐藤隆司, 豊島聰編)
  • Publisher: 南江堂
• [Book] 疾病と病態生理 改定第2版 (2006)
  • Author(s): 酒井秀紀(橋本隆男, 佐藤隆司, 豊島聰編)
  • Total Pages: 396
  • Publisher: 南江堂
• [Book] 「疾病と病態生理」改訂第2版
  • Author(s): 酒井秀紀(橋本隆男, 佐藤隆司, 豊島聰編)
  • Publisher: 南江堂
• [Remarks]
  • URL: http://www.pha.u-toyama.ac.jp/phaphy1/index-j.html
• [Remarks]
  • URL: http://www.pha.u-toyama.ac.jp/phaphyl/index-j.html
• [Remarks]
  • URL: http://www.pha.u-toyama.ac.jp/phaphy1/index-j.html