Molecular mechanisms of formation of cell junctions and polarity

• Project/Area Number: 18390080
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: IRIE Kenji University of Tsukuba, 大学院・人・間総合科学研究科, 教授 (90232628)
• Co-Investigator(Kenkyū-buntansha): NAIKI Takahiro 筑波大学, 大学院・人・間総合科学研究科, 助教 (70420081)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,460,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥1,860,000); Fiscal Year 2008: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2007: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2006: ¥8,400,000 (Direct Cost: ¥8,400,000)
• Keywords: 細胞極性 / 細胞間接着 / ネクチン / アファディン / アドヘレンスジャンクション / タイトジャンクション / Stau1 / RNA局在 / RNA結合タンパク質 / 上皮細胞 / アファデイン / Stau / 筋分化 / 細胞分化 / Staul / カドヘリン / Staufen

Research Abstract

本研究では、ネクチンーアファディン系の細胞間接着による細胞極性の形成機構に焦点を絞り、ネクチンーアファディン系の細胞間接着の形成から接着シグナリングを介したタンパク質および細胞骨格の不均等な局在の形成機構、すなわち細胞極性確立の分子機構を明らかにすることを目的として、研究を行なった。研究の結果、次のような研究成果を得た。ネクチンによるアドヘレンスジャンクションおよびタイトジャンクションの形成には、アファディンとアクチン細胞骨格が必須の役割を果たすことを明らかにした。アネキシンIIをノックダウンした細胞では、タイトジャンクションはカドヘリンによる細胞間接着形成なしに形成されること、タイトジャンクションの形成にはネクチンーアファディン系は必須であることを明らかにした。さらに、アドヘレンスジャンクションおよびタイトジャンクションの形成には、アファディンとPar-3が協調的に機能することを明らかにした。

Research Products

• [Journal Article] hnRNP K interacts with RNA binding motif protein 42 and functions in the maintenance of cellular ATP levels during stress conditions (2009)
  • Author(s): Fukuda, T., Naiki, T., Saito, M., *Irie, K
  • Journal Title: Genes Cells 14巻2号 Pages: 113-128
  • Peer Reviewed
• [Journal Article] hnRNP K interacts with RNA binding motif protein 42 and functions in the maintenance of cellular ATP level during stress conditions. (2009)
  • Author(s): Fukuda et al.
  • Journal Title: Genes to Cells 14 Pages: 113-128
  • Peer Reviewed
• [Journal Article] Stau1 negatively regulates myogenic differentiation in C2C12 cells (2008)
  • Author(s): Yamaguchi, Y., Oohinata, R., Naiki, T., *Irie, K
  • Journal Title: Genes Cells 13巻6号 Pages: 583-592
  • Peer Reviewed
• [Journal Article] Gerber, A.P. Distinct roles for Khd1p in the localization and expression of bud-localized mRNAs in yeast (2008)
  • Author(s): Hasegawa, Y., *Irie, K
  • Journal Title: RNA 14巻 Pages: 1-15
  • Peer Reviewed
• [Journal Article] Distinct roles for Khdlp in the localization and expression of bud-localized mRNAs in yeast (2008)
  • Author(s): Hasegawa et al.
  • Journal Title: RNA 14 Pages: 2333-2347
  • Peer Reviewed
• [Journal Article] Stau1 negatively regulates myogenic differentiation in C2C12 cells. (2008)
  • Author(s): Yamaguchi et al.
  • Journal Title: Genes to Cells 13 Pages: 583-592
  • Peer Reviewed
• [Journal Article] Staul negatively regulates myogenic differentiation in C2C12 cells (2008)
  • Author(s): Yamaguchi, et. al.
  • Journal Title: Genes to Cells (印刷中)
  • Peer Reviewed
• [Journal Article] 酵母におけるmRNA局在と局所的翻訳の制御機構 (2007)
  • Author(s): 入江賢児
  • Journal Title: 実験医学 25(レビュー) Pages: 31-38
• [Journal Article] Up-regulation of loricrin expression by cell adhesion molecule nectin-1 through Rap1-ERK signaling in keratinocytes (2007)
  • Author(s): Wakamatsu, K., Ogita, H., Okabe, N., Irie, K., Tanaka-Okamoto, M., Ishizaki, H., Ishida-Yamamoto, A., Iizuka, H., Miyoshi, J., *Takai, Y
  • Journal Title: J Biol Chem 282巻25号 Pages: 18173-18181
  • Peer Reviewed
• [Journal Article] Cooperative roles of Par-3 and afadin in the formation of adherens and tight junctions (2007)
  • Author(s): Ooshio, T., Fujita, N., Yamada, A., Sato, T., Kitagawa, Y., Okamoto, R., Nakata, S., Miki, A., Irie, K., *Takai, Y
  • Journal Title: J Cell Sci 120巻14号 Pages: 2352-2365
  • Peer Reviewed
• [Journal Article] Up-regulation of loricrin expression by cell adhesion molecule nectin-1 through Rap1-ERK signaling in keratinocytes. (2007)
  • Author(s): Wakamatsu, et. al.
  • Journal Title: Journal of Biological Chemistry 282・25 Pages: 18173-17181
  • Peer Reviewed
• [Journal Article] Cooperative roles of Par-3 and afadin in the formation of adherens and tight junctions. (2007)
  • Author(s): Ooshio, et. al.
  • Journal Title: Journal of Cell Science 120・14 Pages: 2352-2365
  • Peer Reviewed
• [Journal Article] Role of cell adhesion molecule nectin-3 in spermatid development (2006)
  • Author(s): Inagaki, M., Irie, K., Ishizaki, H., Tanaka-Okamoto, M., Miyoshi, J., *Takai, Y
  • Journal Title: Genes Cells 11巻9号 Pages: 1125-1132
  • Peer Reviewed
• [Journal Article] Requirement of nectin, but not cadherin, for formation of claudin-based tight junctions in annexin II-knockdown MDCK cells (2006)
  • Author(s): Yamada, A., Fujita, N., Sato, T., Okamoto, R., Ooshio, T., Hirota, T., Morimoto, K., Irie, K., *Takai, Y
  • Journal Title: Oncogene 25巻37号 Pages: 5085-5102
  • Peer Reviewed
• [Journal Article] Regulation of the assembly and adhesion activity of E-cadherin by nectin and afadin for the formation of adherens junctions in Madin-Darby canine kidney cells (2006)
  • Author(s): Sato, T., Fujita, N., Yamada, A., Ooshio, T., Okamoto, R., Irie, K., *Takai, Y
  • Journal Title: J Biol Chem 281巻8号 Pages: 5288-5299
  • Peer Reviewed
• [Journal Article] Regulation of the assembly and adhesion activity of E-cadherin by nectin and afadin for the frmation of adherens junctions in Madin-Darby canine kidney cells. (2006)
  • Author(s): Sato et al.
  • Journal Title: Journal of Biological Chemistry 281・8 Pages: 5288-5299
• [Journal Article] Requirement of nectin, but not cadherin, for formation of claudin-based tight junctions in annexin II-knockdown MDCK cells (2006)
  • Author(s): Yamada et al.
  • Journal Title: Oncogene 25・37 Pages: 5085-5102
• [Journal Article] Role of cell adhesion molecule nectin-3 in spermatid development (2006)
  • Author(s): Inagaki et al.
  • Journal Title: Genes to Cells 11・9 Pages: 1125-1132
• [Presentation] Distinct roles for Khdlp, the ortholog of mammalian hnRNP K, in the localization and expression of bud-localized mRNAs in yeast. (2008)
  • Author(s): 入江賢児
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2008-07-01
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/public/basic-med/molcellbiol/index.html