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Molecular mechanisms of formation of cell junctions and polarity

Research Project

Project/Area Number 18390080
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

IRIE Kenji  University of Tsukuba, 大学院・人・間総合科学研究科, 教授 (90232628)

Co-Investigator(Kenkyū-buntansha) NAIKI Takahiro  筑波大学, 大学院・人・間総合科学研究科, 助教 (70420081)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥16,460,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2008: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2007: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2006: ¥8,400,000 (Direct Cost: ¥8,400,000)
Keywords細胞極性 / 細胞間接着 / ネクチン / アファディン / アドヘレンスジャンクション / タイトジャンクション / Stau1 / RNA局在 / RNA結合タンパク質 / 上皮細胞 / アファデイン / Stau / 筋分化 / 細胞分化 / Staul / カドヘリン / Staufen
Research Abstract

本研究では、ネクチンーアファディン系の細胞間接着による細胞極性の形成機構に焦点を絞り、ネクチンーアファディン系の細胞間接着の形成から接着シグナリングを介したタンパク質および細胞骨格の不均等な局在の形成機構、すなわち細胞極性確立の分子機構を明らかにすることを目的として、研究を行なった。研究の結果、次のような研究成果を得た。ネクチンによるアドヘレンスジャンクションおよびタイトジャンクションの形成には、アファディンとアクチン細胞骨格が必須の役割を果たすことを明らかにした。アネキシンIIをノックダウンした細胞では、タイトジャンクションはカドヘリンによる細胞間接着形成なしに形成されること、タイトジャンクションの形成にはネクチンーアファディン系は必須であることを明らかにした。さらに、アドヘレンスジャンクションおよびタイトジャンクションの形成には、アファディンとPar-3が協調的に機能することを明らかにした。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (20 results)

All 2009 2008 2007 2006 Other

All Journal Article (18 results) (of which Peer Reviewed: 14 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] hnRNP K interacts with RNA binding motif protein 42 and functions in the maintenance of cellular ATP levels during stress conditions2009

    • Author(s)
      Fukuda, T., Naiki, T., Saito, M., *Irie, K
    • Journal Title

      Genes Cells 14巻2号

      Pages: 113-128

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] hnRNP K interacts with RNA binding motif protein 42 and functions in the maintenance of cellular ATP level during stress conditions.2009

    • Author(s)
      Fukuda et al.
    • Journal Title

      Genes to Cells 14

      Pages: 113-128

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stau1 negatively regulates myogenic differentiation in C2C12 cells2008

    • Author(s)
      Yamaguchi, Y., Oohinata, R., Naiki, T., *Irie, K
    • Journal Title

      Genes Cells 13巻6号

      Pages: 583-592

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gerber, A.P. Distinct roles for Khd1p in the localization and expression of bud-localized mRNAs in yeast2008

    • Author(s)
      Hasegawa, Y., *Irie, K
    • Journal Title

      RNA 14巻

      Pages: 1-15

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distinct roles for Khdlp in the localization and expression of bud-localized mRNAs in yeast2008

    • Author(s)
      Hasegawa et al.
    • Journal Title

      RNA 14

      Pages: 2333-2347

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stau1 negatively regulates myogenic differentiation in C2C12 cells.2008

    • Author(s)
      Yamaguchi et al.
    • Journal Title

      Genes to Cells 13

      Pages: 583-592

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Staul negatively regulates myogenic differentiation in C2C12 cells2008

    • Author(s)
      Yamaguchi, et. al.
    • Journal Title

      Genes to Cells (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 酵母におけるmRNA局在と局所的翻訳の制御機構2007

    • Author(s)
      入江賢児
    • Journal Title

      実験医学 25(レビュー)

      Pages: 31-38

    • Related Report
      2008 Final Research Report
  • [Journal Article] Up-regulation of loricrin expression by cell adhesion molecule nectin-1 through Rap1-ERK signaling in keratinocytes2007

    • Author(s)
      Wakamatsu, K., Ogita, H., Okabe, N., Irie, K., Tanaka-Okamoto, M., Ishizaki, H., Ishida-Yamamoto, A., Iizuka, H., Miyoshi, J., *Takai, Y
    • Journal Title

      J Biol Chem 282巻25号

      Pages: 18173-18181

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cooperative roles of Par-3 and afadin in the formation of adherens and tight junctions2007

    • Author(s)
      Ooshio, T., Fujita, N., Yamada, A., Sato, T., Kitagawa, Y., Okamoto, R., Nakata, S., Miki, A., Irie, K., *Takai, Y
    • Journal Title

      J Cell Sci 120巻14号

      Pages: 2352-2365

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Up-regulation of loricrin expression by cell adhesion molecule nectin-1 through Rap1-ERK signaling in keratinocytes.2007

    • Author(s)
      Wakamatsu, et. al.
    • Journal Title

      Journal of Biological Chemistry 282・25

      Pages: 18173-17181

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cooperative roles of Par-3 and afadin in the formation of adherens and tight junctions.2007

    • Author(s)
      Ooshio, et. al.
    • Journal Title

      Journal of Cell Science 120・14

      Pages: 2352-2365

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of cell adhesion molecule nectin-3 in spermatid development2006

    • Author(s)
      Inagaki, M., Irie, K., Ishizaki, H., Tanaka-Okamoto, M., Miyoshi, J., *Takai, Y
    • Journal Title

      Genes Cells 11巻9号

      Pages: 1125-1132

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Requirement of nectin, but not cadherin, for formation of claudin-based tight junctions in annexin II-knockdown MDCK cells2006

    • Author(s)
      Yamada, A., Fujita, N., Sato, T., Okamoto, R., Ooshio, T., Hirota, T., Morimoto, K., Irie, K., *Takai, Y
    • Journal Title

      Oncogene 25巻37号

      Pages: 5085-5102

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of the assembly and adhesion activity of E-cadherin by nectin and afadin for the formation of adherens junctions in Madin-Darby canine kidney cells2006

    • Author(s)
      Sato, T., Fujita, N., Yamada, A., Ooshio, T., Okamoto, R., Irie, K., *Takai, Y
    • Journal Title

      J Biol Chem 281巻8号

      Pages: 5288-5299

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of the assembly and adhesion activity of E-cadherin by nectin and afadin for the frmation of adherens junctions in Madin-Darby canine kidney cells.2006

    • Author(s)
      Sato et al.
    • Journal Title

      Journal of Biological Chemistry 281・8

      Pages: 5288-5299

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Requirement of nectin, but not cadherin, for formation of claudin-based tight junctions in annexin II-knockdown MDCK cells2006

    • Author(s)
      Yamada et al.
    • Journal Title

      Oncogene 25・37

      Pages: 5085-5102

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Role of cell adhesion molecule nectin-3 in spermatid development2006

    • Author(s)
      Inagaki et al.
    • Journal Title

      Genes to Cells 11・9

      Pages: 1125-1132

    • Related Report
      2006 Annual Research Report
  • [Presentation] Distinct roles for Khdlp, the ortholog of mammalian hnRNP K, in the localization and expression of bud-localized mRNAs in yeast.2008

    • Author(s)
      入江賢児
    • Organizer
      第60回日本細胞生物学会大会
    • Place of Presentation
      パシフイコ横浜
    • Year and Date
      2008-07-01
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/public/basic-med/molcellbiol/index.html

    • Related Report
      2008 Final Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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