The regulatory mechanism of sensor-type transcription factor during mitosis

• Project/Area Number: 18390083
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Shimane University (2007-2008); Nagoya University (2006)
• Principal Investigator: URANO Takeshi Shimane University, 医学部, 教授 (70293701)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥15,470,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥2,070,000); Fiscal Year 2008: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2006: ¥6,500,000 (Direct Cost: ¥6,500,000)
• Keywords: 分裂期キナーゼ / Aurora / p53 / リン酸化モノクローナル抗体 / EB3 / SIAH-1 / ユビキチン化 / タンパク質分解 / リン酸化 / 基質特異性 / セリン / スレオニンキナーゼ / p21 / WAF1

Research Abstract

細胞分裂期の染色体分配など複雑な物理的ストレスをがん細胞が回避する機構を解明する端緒として,様々なストレスに反応する転写因子であるp53 およびその下流の実働分子であるp21/WAF1 が細胞分裂期にAurora によりリン酸化され,それらの機能が細胞分裂期特異的に不活性化されていることを見出した。

Research Products

• [Journal Article] Phosphorylation of Swi6/HP1 regulates transcriptional gene silencing at heterochromatin. (2009)
  • Author(s): Shimada A, Dohke K, Sadaie M, Shinmyozu K,Nakayama J, Urano T and Murakami Y
  • Journal Title: Genes Dev. 23 Pages: 18-23
  • Peer Reviewed
• [Journal Article] Glycosylphosphatidylinositol-anchored arginine-specific ADP-ribosyltransferase7.1(Art7.1) on chicken B cells: the possible role of Art7 in B cell receptor signalling and proliferation. (2009)
  • Author(s): Terashima M, Takahashi M, Shimoyama M,Tanigawa Y, Urano T and Tsuchiya M
  • Journal Title: Mol Cell Biochem 320 Pages: 470-478
  • Peer Reviewed
• [Journal Article] Phosphorylation of Swi6/HP1 regulates transcriptional gene silen cing at heterochromatin. (2009)
  • Author(s): Shimada A.
  • Journal Title: Genes Dev. 23 Pages: 18-23
  • Peer Reviewed
• [Journal Article] Glycosylphosphatidylinositol-anchored arginine-specific ADP-ribos yltransferase7.1 (Art7.1) on chicken B cells : the possible role of Art7 in B cell receptor signalling and proliferation. (2009)
  • Author(s): Terashima M.
  • Journal Title: Mol Cell Biochem. 320 Pages: 470-478
  • Peer Reviewed
• [Journal Article] Gene silencing in cancer by histone H3 lysine 27 trimethylation independent of promoter DNA methylation. (2008)
  • Author(s): Kondo Y, Shen L, Cheng AS, Ahmed S,Boumber Y, Charo C, Yamochi T, Urano T,Furukawa K, Kwabi-Addo B, Gold DL,Sekido Y, Huang TH and Issa JP
  • Journal Title: Nature Genet 40 Pages: 741-750
  • Peer Reviewed
• [Journal Article] Heterochromatin and RNAi Are Required to Establish CENP-A Chromatin at Centromeres. (2008)
  • Author(s): Folco HD, Pidoux AL, Urano T, and Allshire RC
  • Journal Title: Science 319 Pages: 94-97
  • Peer Reviewed
• [Journal Article] Cdc2p controls the forkhead transcription factor Fkh2p by phosphorylation during sexual differentiation in fission yeast. (2008)
  • Author(s): Shimada M, Yamada-Namikawa C,Murakami-Tonami Y, Yoshida T, Nakanishi M, Urano T, and Murakami H
  • Journal Title: EMBO J 27 Pages: 132-142
  • Peer Reviewed
• [Journal Article] Fission yeast chromatin assembly factor 1 assists in the replication-coupled maintenance of heterochromatin. (2008)
  • Author(s): Dohke K, Miyazaki S, Tanaka K, Urano T,Grewal SI and Murakami Y
  • Journal Title: Genes Cells 13 Pages: 1027-1043
  • Peer Reviewed
• [Journal Article] Splicing factors facilitate RNAi-directed silencing in fission yeast. (2008)
  • Author(s): Bayne EH
  • Journal Title: Science 322 Pages: 602-606
  • Peer Reviewed
• [Journal Article] Gene silencing in cancer by histone H3 lysine 27 trimethylation independent of promoter DNA methylation. (2008)
  • Author(s): Kondo Y.
  • Journal Title: Nature Genet. 40 Pages: 741-750
  • Peer Reviewed
• [Journal Article] Heterochromatin and RNAi Are Required to Establish CENP-A Chromatin at Centromeres. (2008)
  • Author(s): Folco HD.
  • Journal Title: Science 319 Pages: 94-97
  • Peer Reviewed
• [Journal Article] Cdc2p controls the forkhead transcription factor Fkh2p by phosp horylation during sexual differentiation in fission yeast. (2008)
  • Author(s): Shimada M
  • Journal Title: EMBO J. 27 Pages: 132-142
  • Peer Reviewed
• [Journal Article] Focal adhesion kinase as well as p130Cas and paxillin is crucially involved in the enhanced malignant properties under expressi on of ganglioside GD3 in melanoma cells. (2008)
  • Author(s): Hamamura K.
  • Journal Title: Biochim Biophys Acta. 1780 Pages: 513-519
  • Peer Reviewed
• [Journal Article] Fission yeast chromatin assembly factor 1 assists in the replication-coupled maintenance of heterochromatin. (2008)
  • Author(s): Dohke K.
  • Journal Title: Genes Cells. 13 Pages: 1027-1043
  • Peer Reviewed
• [Journal Article] Impaired hypoglossal nerve regeneration in mutant mice lacking complex gangliosides : down-regulation of neurotrophic factors and receptors as possible mechanisms. (2008)
  • Author(s): Kittaka D.
  • Journal Title: Glycobiology 18 Pages: 509-516
  • Peer Reviewed
• [Journal Article] Heterochromatin and RNAi Are Required to Establish CENP-A Chromatin at Centromeres. (2008)
  • Author(s): Folco H. D.
  • Journal Title: Science 319 Pages: 94-97
  • Peer Reviewed
• [Journal Article] Cdc2p controls the forkhead transcription factor Fkh2p by phosphorylation during sexual differentiation in fission yeast. (2008)
  • Author(s): Shimada M.
  • Journal Title: EMBO J. 27 Pages: 132-142
  • Peer Reviewed
• [Journal Article] Focal adhesion kinase as well as pl30Cas and paxillin is crucially in volved in the enhanced malignant properties under expression of gang lioside GD3 in melanoma cells. (2008)
  • Author(s): Hamamura K
  • Journal Title: Biochim Biophys Acta. 1780 Pages: 513-519
  • Peer Reviewed
• [Journal Article] Overexpression of caveolin-1 in a human melanoma cell line results in dispersion of ganglioside GD3 from lipid rafts and alteration of leading edes,leading to attenuation of malignant proerties. (2008)
  • Author(s): Nakashima H
  • Journal Title: Cancer Sci. 98 Pages: 512-520
  • Peer Reviewed
• [Journal Article] 小胞体による核膜の再形成 (2008)
  • Author(s): 伴 玲子
  • Journal Title: 生化学 80 Pages: 194-199
• [Journal Article] The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin. (2007)
  • Author(s): Trewick SC, Minc E, Antonelli R, Urano T,and Allshire RC
  • Journal Title: EMBO J. 26 Pages: 4670-4682
  • Peer Reviewed
• [Journal Article] Gene amplification and overexpression of PRDM14 in breast cancers. (2007)
  • Author(s): Nishikawa N, Toyota M, Suzuki H,Honma T, Fujikane T, Ohmura T,Nishidate T, Ohe-Toyota M, Maruyama R,Sonoda T, Sasaki Y, Urano T, Imai K,Hirata K, and Tokino T
  • Journal Title: Cancer Res 67 Pages: 9649-9657
  • Peer Reviewed
• [Journal Article] Analysis of the role of Aurora B on the chromosomal targeting of condensin I. (2007)
  • Author(s): Takemoto A, Murayama A, Katano M,Urano T, Furukawa K, Yokoyama S,Yanagisawa J, Hanaoka F and Kimura K
  • Journal Title: Nucleic Acids Res 35 Pages: 2403-2412
  • Peer Reviewed
• [Journal Article] Role of DNA methylation and histone H3 Lysine 27 methylation in tissue-specific imprinting of mouse Grb10. (2007)
  • Author(s): Yamasaki-Ishizaki Y, Kayashima T,Mapendano CK, Soejima H, Ohta T,Masuzaki H, Kinoshita A, Urano T,Yoshiura KI, Matsumoto N, Ishimaru T,Mukai T, Niikawa N and Kishino T
  • Journal Title: Mol. Cell. Biol. 27 Pages: 732-742
  • Peer Reviewed
• [Journal Article] Overexpression of caveolin-1 in a human melanoma cell line results in dispersion of ganglioside GD3 from lipid rafts and alteration of leading edges, leading to attenuation of malignant properties. (2007)
  • Author(s): Nakashima H, Hamamura K, Houjou T,Taguchi R, Yamamoto N, Mitsudo K,Tohnai I, Ueda M, Urano T, Furukawa K,Furukawa K
  • Journal Title: Cancer Sci 98 Pages: 512-520
  • Peer Reviewed
• [Journal Article] The JmjC domain protein Epel prevents unregulated assembly and disassembly of heterochromatin. (2007)
  • Author(s): Trewick S. C.
  • Journal Title: EMBO J. 26 Pages: 4670-4682
  • Peer Reviewed
• [Journal Article] Gene amplification and overexpression of PRDM14 in breast cancers. (2007)
  • Author(s): Nishikawa N
  • Journal Title: Cancer Res. 67 Pages: 9649-9657
  • Peer Reviewed
• [Journal Article] Analysis of the role of Aurora B on the chromosomal targeting of condensin I. (2007)
  • Author(s): Takemoto A
  • Journal Title: Nucleic Acids Res. 35 Pages: 2403-2412
  • Peer Reviewed
• [Journal Article] Role of DNA methylation and histone H3 Lysine 27 methylation in tissue-specific imprinting of mouse Grb10. (2007)
  • Author(s): Yamasaki-Ishizaki Y
  • Journal Title: Mol. Cell. Biol. 27 Pages: 732-742
  • Peer Reviewed
• [Journal Article] Identification and expression of a sialyltransferase responsible for the synthesis of disialylgalactosylgloboside in normal and malignant kidney cells: down-regulation of ST6Ga1NAc VI in renal cancers. (2007)
  • Author(s): Senda M
  • Journal Title: Biochem. J. 402 Pages: 459-470
  • Peer Reviewed
• [Journal Article] Role of DNA methylation and histone H3 Lysine 27 methylation in tissue-specific imprinting of mouse Grb10 (2007)
  • Author(s): Yamasaki-Ishizaki Y.
  • Journal Title: Mol. Cell. Biol. 27 Pages: 732-742
• [Journal Article] Analysis of the role of Aurora B on the chromosomal targeting of condensin I (2007)
  • Author(s): Takemoto A.
  • Journal Title: Nucleic Acids Res. (in press)
• [Journal Article] Phospho-regulation of human Protein Kinase Aurora-A: Analysis using anti-phospho-Thr288 monoclonal antibodies. (2006)
  • Author(s): Ohashi S, Sakashita G, Ban R, Nagasawa M, Matsuzaki H, Murata Y, Taniguchi H,Shima H, Furukawa K and Urano T
  • Journal Title: Oncogene 25 Pages: 7691-7702
  • Peer Reviewed
• [Journal Article] Complex formation of Plk1 and INCENP required for metaphase-anaphase transition. (2006)
  • Author(s): Goto H, Kiyono T, Tomono Y, Kawajiri A, Urano T, Furukawa K, Nigg EA and Inagaki M
  • Journal Title: Cell Biol 8 Pages: 180-187
  • Peer Reviewed
• [Journal Article] Nucleophosmin (NPM): A versatile molecule associated with hematological malignancies. (2006)
  • Author(s): Naoe T, Suzuki T, Kiyoi H and Urano T
  • Journal Title: Cancer Sci 97 Pages: 963-969
• [Journal Article] Expression profile of LIT1/KCNQ1OT1 and epigenetic status at the KvDMR1 in colorectal cancers. (2006)
  • Author(s): Nakano S, Murakami K, Meguro M, Soejima H, Higashimoto K, Urano T,Kugoh H, Mukai T, Ikeguchi M and Oshimura M
  • Journal Title: Cancer Sci 97 Pages: 1147-1154
  • NAID: 10019376824
  • Peer Reviewed
• [Journal Article] Targeted disruption of Gb3/CD77 synthase gene resulted in the complete deletion of globo-series glycosphingolipids and loss of sensitivity to verotoxins. (2006)
  • Author(s): Okuda T, Tokuda N, Numata S, Ito M, Ohta M, Kawamura K, Wiels J, Urano T, Tajima O, Furukawa K and Furukawa K
  • Journal Title: J. Biol. Chem 281 Pages: 10230-10235
  • Peer Reviewed
• [Journal Article] Metastatic potential of mouse Lewis lung cancer cells is regulated via ganglioside GM1 by modulating the matrix metalloprotease-9 localization in lipid rafts. (2006)
  • Author(s): Zhang Q, Furukawa K, Chen HH, Sakakibara T, Urano T and Furukawa K
  • Journal Title: J. Biol. Chem 281 Pages: 18145-18155
  • Peer Reviewed
• [Journal Article] Phospho-regulation of human protein kinase Aurora-A : analysis using anti-phospho-Thr288 monoclonal antibodies (2006)
  • Author(s): Ohashi S.
  • Journal Title: Oncogene 25 Pages: 7691-7702
• [Journal Article] Nucleophosmin (NPM) : A versatile molecule associated with hematological malignancies (2006)
  • Author(s): Naoe T.
  • Journal Title: Cancer Sci. 97 Pages: 963-969
• [Journal Article] Expression profile of LIT1/KCNQ1OT1 and epigenetic status at the KvDMR1 in colorectal cancers (2006)
  • Author(s): Nakano S.
  • Journal Title: Cancer Sci. 97 Pages: 1147-1154
• [Journal Article] がん治療における新規標的 : 分裂期キナーゼAurora (2006)
  • Author(s): 伴 玲子
  • Journal Title: がん分子標的治療 4 Pages: 254-265
• [Presentation] Calcium/Calmodulin Binds to and Inhibits Aurora-A Kinase Activity at Anaphase. (2008)
  • Author(s): Takeshi Urano
  • Organizer: 48^th ASCB
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2008-12-14
• [Presentation] 癌転移に関与する糖転移酵素の同定 (2008)
  • Author(s): 松本康之
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] A4galtは血管内皮特異的にLPS応答性を制御する (2008)
  • Author(s): 近藤 佑史
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 新規シンタキシンフアミリーの同定と機能解析 (2008)
  • Author(s): 坂下 暁介
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 分裂期キナーゼAurora-BのSUMO化による制御機構の解析 (2008)
  • Author(s): 伴 玲子
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Identification of a new regulatory domain in arginine-specific ADP-ribosyltransferase 7 (Art7). (2008)
  • Author(s): 寺嶋 正治
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 癌転移に関与する糖転移酵素の同定〜その糖鎖産物上のシアル酸の作用機構〜 (2008)
  • Author(s): 松本康之
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] 癌転移に関与する糖転移酵素の同定:複合糖鎖の変化における作用機構の解析 (2007)
  • Author(s): 松本康之
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 分裂酵母におけるヘテロクロマチンタンパク質のリン酸化制御 (2007)
  • Author(s): 島田 篤
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] 微小管プラス端集積因子EB3のタンパク質分解機構 (2007)
  • Author(s): 伴 玲子
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Molecular characterization of Art7, glycosylphosphatodylinositol-anchored arginine-specific ADP-ribosvltransferase 7-insights into catalytic domains (2007)
  • Author(s): 寺嶋正治
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] TGF-β1 suppresses cAMP-induced nitric oxide synthase expression by im peding the nucleocytoplasmic shuttling of protein kinase A (2007)
  • Author(s): 成相裕子
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Involvement of integrins in the malignant phenotypes of human melanom as enhanced by ganglioside GD3 (2007)
  • Author(s): 大川裕樹
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] 癌転移に関与する糖転移酵素の同定と作用機構の解析 (2007)
  • Author(s): 松本康之
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] 細胞周期を制御する分裂期キナーゼAurora (2007)
  • Author(s): 浦野 健
  • Organizer: 第16回日本アポトーシス研究会
  • Place of Presentation: 千葉
  • Year and Date: 2007-08-04
• [Presentation] 細胞周期を制御する分裂期キナーゼAurora (2007)
  • Author(s): 浦野 健
  • Organizer: 第16回日本アポトーシス研究会(招待講演)
  • Place of Presentation: 千葉
  • Year and Date: 2007-08-04
• [Presentation] Calcium/Calmodulin Binds to and Inhibits Aurora-A Kinase Activity at Anaphase (2000)
  • Author(s): Reiko Ban and Takeshi Urano
  • Organizer: 48^<th> ASCB
  • Place of Presentation: San Francisco (USA)
• [Book] 細胞周期集中マスター 北川雅敏編、-レビュー編第1 章細胞周期を制御するリン酸化酵素- (2006)
  • Author(s): 浦野 健
  • Publisher: 株式会社羊土社
• [Book] 細胞周期集中マスター -第1章 細胞周期を制御するリン酸化酵素(レビュー編)-(北川雅敏編) (2006)
  • Author(s): 浦野 健
  • Total Pages: 154
  • Publisher: 羊土社
• [Remarks] ホームページ等
  • URL: http://www.med.shimane-u.ac.jp/biochem2/index.html
• [Remarks]
  • URL: http://www.med.shimane-u.ac.jp/biochem2/index.html
• [Remarks]
  • URL: http://www.med.shimane-u.ac.jp/biochem2/index.html