Regulatory Mechanism for the reactive oxygen-producing enzyme family Nox

• Project/Area Number: 18390090
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Kyushu University
• Principal Investigator: SUMIMOTO Hideki Kyushu University, Medical Institute of Bioregulation, Professor (30179303)
• Co-Investigator(Kenkyū-buntansha): TAKEYA Ryu KYUSHU UNIVERSITY, Medical Institute of Bioregulation, Research Professor (50335981) ; KAMAKURA Sachiko KYUSHU UNIVERSITY, Medical Institute of Bioregulation, Research Associate (80398081)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥16,720,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥1,920,000); Fiscal Year 2007: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000); Fiscal Year 2006: ¥8,400,000 (Direct Cost: ¥8,400,000)
• Keywords: reactive oxygen species / protein / signal transduction / enzyme / NADPH oxidase / 活性酸素 / シグナル云達 / NADPHオキシダー / NAPDHオキシダーゼ

Research Abstract

On the mechanism of the reactive oxygen-producing enzyme family Nox (NADPH oxidase), we obtained the following results during the course of this project. (1) We showed that an intramolecular interaction between the PX and PB1 domains of p40pax, a protein involved in Nox2 activation, regulates translocation of this protein to the phagosomal membrane, and also determined the crystal structure of the full-length p40phax. (2) We identified Nox2 regions, which participate in electron transfer in Nox2 and Nox2 binding to p22phax. (3) We demonstrated that Noxo1, an essential activator of Nox1, is kept in an inactive form via an intramolecular interaction, which is released by posttranslational modifications. (4) We clarified that the small GTPase Rac functions as an activator of Nox1 and Nox3 by interacting with Noxa1 and p67phax., respectively. (5) We also showed that Rac3 is capable of acting as a regulator of Nox1, Nox2, and Nox3, and that none of the activations of Nox1-3 by Rac1-3 requires the insert helix, a region specific to the Rho-family GTPases including Rac.

Research Products

• [Journal Article] Characterization of teleost phagocyte NADPH oxidase:molecular cloning and expression analysis of carp(Cyprinus carpio)phagocyte NADPH oxidase (2008)
  • Author(s): Mayumi, M., Takeda, Y., Hoshiko, M., Serada, K., Murata, M., Moritomo, T., Takizawa, F., Kobayashi, I., Araki, K., Nakanishi, T., and Sumimoto, H.
  • Journal Title: Mol.Immunol 45 Pages: 1720-1731
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The mammalian formin FHOD1 is activated via phosphorylation by ROCK and mediates thrombin-induced stress fibre formation in endothelial cells (2008)
  • Author(s): Takeya, R., Taniguchi, K., Narumiya, S., and Sumimoto, H.
  • Journal Title: EMBO J. 27 Pages: 618-628
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Structure,regulation,and evolution of Nox-family NADPH oxidases that produce,reactive oxygen species (2008)
  • Author(s): Sumimoto, H.
  • Journal Title: FEBS J. (in press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Characterization of teleost phagocyte NADPH oxidase : molecular cloning and expression analysis of carp (Cyprinus carpio) phagocyte NADPH oxidase. (2008)
  • Author(s): Mayumi, M., Takeda, Y., Hoshiko, M., Serada, K., Murata, M., Moritomo, T., Takizawa, F., Kobayashi, I., Araki, K., Nakanishi, T., Sumimoto, H
  • Journal Title: Mol. Immunol 45 Pages: 1720-1731
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The mammalian formin FHOD1 is activated via phosphorylation by ROCK and mediates thrombin-induced stress fibre formation in endothelial cells. (2008)
  • Author(s): Takeya, R., Taniguchi, K., Narumiya, S., Sumimoto, H
  • Journal Title: EMBO J 27 Pages: 618-628
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enhanced expression of NAPDH oxidase Nox4 in human gliomas and its roles in cell proliferation and survival. (2008)
  • Author(s): Shono, T., Yokoyama, N., Uesaka, T., Kuroda, J., Takeya, R., Yamasaki, T., Amano, T., Mizoguchi, M., Suzuki, S. O., Niiro, H., Miyamoto, K., Akashi, K., Iwaki, T., Sumimoto, H., Sasaki, T
  • Journal Title: Int. J. Cancer (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mechanism for activation of Nox Family. (2008)
  • Author(s): Maehara, Y., Miyano, K., Sumimoto, H.
  • Journal Title: G. I. Research 16(in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of teleost phagocyte NADPH oxidase:molecular cloning and expression analysis of carp (Cyprinus carpio)phagocyte NADPH oxidase. (2008)
  • Author(s): Mayumi, M., Takeda, Y., Hoshiko, M., Serada, K., Murata, M., Moritomo, T., Takizawa, F., Kobayashi, L, Araki, K., Nakanishi, T., andSumimoto, H.
  • Journal Title: Mol.Immunol. 45 Pages: 1720-1731
  • Peer Reviewed
• [Journal Article] The mammalian formin FHODI is activated via phosphorylation by ROCK and mediates thrombin-induced stress fibre formation in endothelial cells. (2008)
  • Author(s): Takeya, R., Taniguchi, K., Narumiya, S., and Sumimoto, H.
  • Journal Title: EMBO J. 27 Pages: 618-628
  • Peer Reviewed
• [Journal Article] Enhanced expression of NAPDH oxidase Nox4 in human gliomas and its roles in cell proliferation and survival. (2008)
  • Author(s): Shono, T., Yokoyama, N., Uesaka, T., Kuroda, J., Takeya, R., Yamasaki, T., Amano, T., Mizoguchi, M., Suzuki, S.O., Niiro, H., Miyamoto, K., Akashi, K, Iwaki, T., Sumimoto, H., and Sasaki, T.
  • Journal Title: Int.J.Cancer. (in press)
  • Peer Reviewed
• [Journal Article] Noxファミリーメンバーの活性調節機構. (2008)
  • Author(s): 前原 優一, 宮野 佳, 住本 英樹
  • Journal Title: G.I.Research 16(印刷中)
  • Peer Reviewed
• [Journal Article] Structure and function of the PB1 domain,a protein-interaction module conserved in animals,fungi,amoebas,and plants (2007)
  • Author(s): Sumimoto, H., Kamakura, S., and Ito, T.
  • Journal Title: Sci.STKE 2007 re6
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Interaction between the SH3 domains and C-terminal proline-rich region in NADPH oxidase organizer 1(Noxol) (2007)
  • Author(s): Yamamoto, A., Kami, K., Takeya, R., and Sumimoto, H.
  • Journal Title: Biochem.Biophys.Res.Commun 352 Pages: 560-565
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Full-length p40^<phox> structure suggests a basis for regulation mechanism of its membrane binding (2007)
  • Author(s): Honbou, K., Minakami, R., Yuzawa, S., Takeya, R., Suzuki, N.N., Kamakura, S., Sumimoto, H., and Inagaki, F.
  • Journal Title: EMBO J. 26 Pages: 1176-1186
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Transient receptor potential(TRP)protein 7 acts as a Gprotein-activated Ca^<2+> channel mediating angiotensin II-induced myocardial apoptosis (2007)
  • Author(s): Satoh, S., Tanaka, H., Ueda, Y., Oyama, J.I., Sugano, M., Sumimoto, H., Mori, Y., Makino, N.
  • Journal Title: Mol.Cell.Biochem 294 Pages: 205-215
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A regulated adaptor function of p40^<phox>:distinct p67^<phox> membrane targeting by p40^<phox> and by p47^<phox> (2007)
  • Author(s): Ueyama, T., Tatsuno, T., Kawasaki, T., Tsujibe, S., Shirai, Y., Sumimoto, H., Leto, T.L., and Saito, N.
  • Journal Title: Mol.Biol.Cell 18 Pages: 441-454
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Noxl redox-signaling mediates oncogenic Ras-induced disruption of stress fibers and focal adhesions by down-regulating Rho. (2007)
  • Author(s): Shinohara, M., Shang, W.H., Kubodera, M., Harada, S., Mitsushita, J., Kato, M., Miyazaki, H., Sumimoto, H., and Kamata, T.
  • Journal Title: J.Biol.Chem 282 Pages: 17640-17648
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Pitavastatin ameliorates albuminuria and renal mesangial expansion via down-regulation of NOX4 in db/db mice (2007)
  • Author(s): Fujii, M., Inoguchi, T., Maeda, Y., Sasaki, S., Sawada, F., Saito, R., Kobayashi, K., Sumimoto, H., and Takayanagi, R.
  • Journal Title: Kidney Int 72 Pages: 473-480
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Role of the small GTPase Rac in p22^<phox>dependent NADPH oxidases (2007)
  • Author(s): Miyano, K., and Sumimoto, H.
  • Journal Title: Biochimie 89 Pages: 1133-1144
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Structure and function of the PB1 domain, a protein-interaction module conserved in animals, fungi, amoebas, and plants. (2007)
  • Author(s): Sumimoto, H., Kamakura, S., Ito, T
  • Journal Title: Sci. STKE2007 re6
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interaction between the SH3 domains and C-terminal proline-rich region in NADPH oxidase organizer 1 (Noxo1). (2007)
  • Author(s): Yamamoto, A., Kami, K., Takeya, R., Sumimoto, H
  • Journal Title: Biochem. Biophys. Res. Commun 352 Pages: 560-565
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Full-length p40phox structure suggests a basis for regulation mechanism of its membrane binding. (2007)
  • Author(s): Honbou, K., Minakami, R., Yuzawa, S., Takeya, R., Suzuki, N. N., Kamakura, S., Sumimoto, H., Inagaki, F
  • Journal Title: EMBO J 26 Pages: 1176-1186
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transient receptor potential (TRP) protein 7 acts as a G protein-activated Ca^<2+> channel mediating angiotensin II-induced myocardial apoptosis. Mol. Cell. (2007)
  • Author(s): Satoh, S., Tanaka, H., Ueda, Y., Oyama, J. I., Sugano, M., Sumimoto, H., Mori, Y., Makino, N
  • Journal Title: Biochem 294 Pages: 205-215
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A regulated adaptor function of p40phox : distinct p67phox membrane targeting by p40phox and by p47phox (2007)
  • Author(s): Ueyama, T., Tatsuno, T., Kawasaki, T., Tsujibe, S., Shirai, Y., Sumimoto, H., Leto, T. L., , Saito, N
  • Journal Title: Mol. Biol. Cell 18 Pages: 441-454
  • NAID: 120000941643
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Nox1 redox-signaling mediates oncogenic Ras-induced disruption of stress fibers and focal adhesions by down-regulating Rho. (2007)
  • Author(s): Shinohara, M., Shang, W. H., Kubodera, M., Harada, S., Mitsushita, J., Kato, M., Miyazaki, H., Sumimoto, H., Kamata, T
  • Journal Title: J. Biol. Chem 282 Pages: 17640-17648
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Pitavastatin ameliorates albuminuria and renal mesangial expansion via down-regulation of NOX4 in db/db mice. (2007)
  • Author(s): Fujii, M., Inoguchi, T., Maeda, Y., Sasaki, S., Sawada, F., Saito, R., Kobayashi, K., Sumimoto, H., Takayanagi, R
  • Journal Title: Kidney Int 72 Pages: 473-480
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of the small GTPase Rac in p22phox dependent NADPH oxidases. (2007)
  • Author(s): Miyano, K., Sumimoto, H
  • Journal Title: Biochimie 89 Pages: 1133-1144
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mechanism for Regulation of Nox Family. (2007)
  • Author(s): Miyano, K., Minakami, R., Sumimoto, H
  • Journal Title: Rinsho Kensa 51 Pages: 1111-1115
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure and function of the PB1 domain,a protein-interaction module conserved in animals,fungi,amoebas,and plants. (2007)
  • Author(s): Sumimoto, H., Kamakura, S., and Ito, T.
  • Journal Title: Sci. STKE2007. re6
  • Peer Reviewed
• [Journal Article] Interaction between the SH3 domains and C-terminal proline-rich region in NADPH oxidase organizer1 (Noxol). (2007)
  • Author(s): Yamamoto, A., Kami, K., Takeya, R., and sumimoto, H.
  • Journal Title: Biochem.Biophys.Res.Commun. 352 Pages: 560-565
  • Peer Reviewed
• [Journal Article] Full-length p40^<phox> structure suggest a basis for regulation mechanism of its membrane binding. (2007)
  • Author(s): Honbou, K., Minakami, R., Yzawa, S., Takeya, R., Suzuki, N.N., kamakura, S., Sumimoto, H., and Inagaki, F.
  • Journal Title: EMBO J. 26 Pages: 1176-1186
  • Peer Reviewed
• [Journal Article] Transient receptor potential (TRP) protein 7 acts as a Gprotein-activatedCa^<2+>channelmediating angiotensin II-induced myocardial apoptosis. (2007)
  • Author(s): Satoh, S., Tanaka, H., Ueda, Y., Oyama, J.L., Sugano, M., Sumimoto, H., Mori, Y., Makino, N.
  • Journal Title: Cell.Biochem. 294 Pages: 205-215
  • Peer Reviewed
• [Journal Article] A regulated adaptor function of p40^<phox>:distinctせ67^<phox> membrane targeting by p40^<phox> and by p47^<phox>. (2007)
  • Author(s): Ueyama, T., Tatsuno, T., Kawasaki, T., Tsujibe, S., Shirai, Y., Sumimoto, H., Leto, T.L., andSaito, N.
  • Journal Title: Mol.Biol.cell 18 Pages: 441-454
  • Peer Reviewed
• [Journal Article] Nox1 redox-signaling mediates oncogenic Ras-induced disruption of stress fibers andfocal adhesions by down-regulating Rho. (2007)
  • Author(s): Shinohara, M., Shang, W.H., Kubodera, M., Harada, S., Mitsushita, J., Kato, M., Miyazaki, H., Sumimoto, H., and Kamata, T.
  • Journal Title: J.Biol.Chem. 282 Pages: 17640-17648
  • Peer Reviewed
• [Journal Article] Pitavastatin ameliorates albuminuria and renal mesangial expansion via down-regulation of NOX4 in db/db mice. (2007)
  • Author(s): Fujii, M., Inoguchi, T., Maeda, Y., Sasaki, S., Sawada, F., Saito, R., Kobayashi, K., Sumimoto, H., and Takayanagi, R.
  • Journal Title: Kidney Int. 72 Pages: 473-480
  • Peer Reviewed
• [Journal Article] Role of the small GTPase Rac in p22p^<phox>-dependent NADPH oxidases. (2007)
  • Author(s): Miyano, K., and Sumimoto H.
  • Journal Title: Biochimie. 89 Pages: 1133-1144
  • Peer Reviewed
• [Journal Article] Nox ファミリーの調節機構. (2007)
  • Author(s): 宮野 佳, 水上 令子, 住本 英樹.
  • Journal Title: 臨床検査 51 Pages: 1111-1115
  • Peer Reviewed
• [Journal Article] Transient receptor potential (TRP) protein 7 acts as a G protein-activated Ca^<2+> channel mediating angiotensin II-induced myocardial apoptosis. (2007)
  • Author(s): Satoh, S., Tanaka, H., Ueda, Y., Oyama, J.I., Sugano, M., Sumimoto, H., Mori, Y., Makino, N.
  • Journal Title: Mol. Cell. Biochem. 294 Pages: 205-215
• [Journal Article] Interaction between the SH3 domains and C-terminal proline-rich region in NADPH oxidase organizer 1(Noxo1). (2007)
  • Author(s): Yamamoto, A., Kami, K., Takeya, R., Sumimoto, H.
  • Journal Title: Biochem. Biophys. Res Commun. 352 Pages: 560-565
• [Journal Article] A regulated adaptor function of p40^<phox> : distinct p67^<phox> membrane targeting by p40^<phox> and by p47^<phox>. (2007)
  • Author(s): Ueyama, T., Tatsuno, T., Kawasaki, T., Tsujibe, S., Shirai, Y., Sumimoto, H., Leto, T.L., Saito, N.
  • Journal Title: Mol. Biol. Cell 18 Pages: 441-454
• [Journal Article] Full-length p40^<phox> structure suggests a' basis for regulation mechanism of its membrane binding. (2007)
  • Author(s): Honbou, K., Minakami, R., Yuzawa, S., Takeya, R., Suzuki, N.N., Kamakura, S., Sumimoto, H., Inagaki, F.
  • Journal Title: EMBO J. 26 Pages: 1176-1186
• [Journal Article] Nox1 redox-signaling mediates oncogenic Ras-induced disruption of stress fibers and focal adhesions by down-regulating Rho. (2007)
  • Author(s): Shinohara, M., Shang, W.H., Kubodera, M., Harada, S., Mitsushita, J., Kato, M., Miyazaki, H., Sumimoto, H., Kamata, T.
  • Journal Title: J. Biol. Chem. 282(in press)
• [Journal Article] Direct involvement of the small GTPase Rac inactivation of the superoxide-producing NADPH oxidase Noxl (2006)
  • Author(s): Miyano, K., Ueno, N., Takeya, R., and Sumimoto, H.
  • Journal Title: J.Biol.Chem 281 Pages: 21857-21868
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Activation of the superoxide-producing phagocyte NADPH oxidase requires co-operation between the tandem SH3 domains of p47^<phox> in recognition of a polyroline tye II helix and an adjacent α-helix of p22^<phox> (2006)
  • Author(s): Nobuhisa, I., Takeya, R., Ogura, K., Ueno, N., Kohda, D., Inagaki, F., and Sumimoto, H.
  • Journal Title: Biochem.J. 396 Pages: 183-192
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Regulation of superoxide-producing NADPH oxidases in nonphagocytic cells (2006)
  • Author(s): Takeya, R., Ueno, N., and Sumimoto, H.
  • Journal Title: Methods Enzymol 406 Pages: 456-468
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] NMR solution structure of the tandem SH3 domains of p47^<phox> complexed with ap22^<phox>-derived proline-rich peptide (2006)
  • Author(s): Ogura, K., Nobuhisa, I., Yuzawa, S., Takeya, R., Torikai, S., Saikawa, K., Sumimoto, H., and Inagaki, F.
  • Journal Title: J.Biol.Chem 281 Pages: 3660-3668
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3 (2006)
  • Author(s): Izaki, T., Kamakura, S., Kohjima, M., and Sumimoto, H.
  • Journal Title: Biochem.Biophys.Res.Commun 341 Pages: 1001-1006
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Regulation of novel superoxide-producing NAD(P)H oxidases (2006)
  • Author(s): Takeya, R., and Sumimoto, H.
  • Journal Title: Antioxid.Redox Signal 8 Pages: 1523-1532
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Expression and function of Noxolγ,an alternative splicing form of the NADPH oxidase organizer 1 (2006)
  • Author(s): Takeya, R., Taura, M., Yamasaki, T., Naito, S., and Sumimoto, H.
  • Journal Title: FEBS J. 273 Pages: 3663-3677
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Phagocytosis-coupled activation of the superoxide-producing phagocyte oxidase,a member of the NADPH oxidase(Nox)family (2006)
  • Author(s): Minakami, R., and Sumimoto, H.
  • Journal Title: Int.J.Hematol 84 Pages: 193-198
  • NAID: 10018303702
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Expression of isoforms of NADPH oxidase components in rat pancreatic islets (2006)
  • Author(s): Uchizono, U., Takeya, R., Iwase, M., Sasaki, N., Oku, M., Imoto, H., Iida, M., and Sumimoto, H.
  • Journal Title: Life Sci. 80 Pages: 133-139
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic analysis of p40^<phox>,a regulatory subunit of NADPH oxidase (2006)
  • Author(s): Honbou K, Yuzawa S, Suzuki NN, Fujioka Y, Sumimoto H, Inagaki F.
  • Journal Title: Acta Crystallograph Sect F Struct Biol Cryst Commun 62 Pages: 1018-1020
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Increased expression of gp91^<phox> homologues of NAD(P)H oxidase in the aortic media during chronic hypertension;involvement of the rennin-angiotensin system (2006)
  • Author(s): Onohara, N., Nishida, M., Inoue, R., Kobayashi, H., Sumimoto, H., Sato, Y., Mori, Y., Nagao, T., and Kurose, H.
  • Journal Title: EMBO J. 25 Pages: 5305-5316
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic analysis of p40^<phox>,a regulatory subunit of NADPH oxidase (2006)
  • Author(s): Akasaki, T., Ohya, Y., Kuroda, J., Eto, K., Abe, I., Sumimoto, H., and Iida, M.
  • Journal Title: Hypertens.Res. 29 Pages: 813-820
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Enhanced expression of NAPDH oxidase Nox4 in human gliomas and its roles in cell proliferation and survival (2006)
  • Author(s): Shono, T., Yokoyama, N., Uesaka, T., Kuroda, J., Takeya, R, Yamasaki, T., Amano, T., Mizoguchi, M., Suzuki, S.O., Niiro, H., Miyamoto, K., Akashi, K., Iwaki, T., Sumimoto, H., and Sasaki, T.
  • Journal Title: Int.J.Cancer (in press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Direct involvement of the small GTPase Rac in activation of the superoxide-producing NADPH oxidase Nox1. (2006)
  • Author(s): Miyano, K., Ueno, N., Takeya, R., Sumimoto, H
  • Journal Title: J. Biol. Chem 281 Pages: 21857-21868
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Activation of the superoxide-producing phagocyte NADPH oxidase requires co-operation between the tandem SH3 domains of p47phox in recognition of a polyproline type II helix and an adjacent a-helix of p22phax (2006)
  • Author(s): Nobuhisa, I., Takeya, R., Ogura, K., Ueno, N., Kohda, D., Inagaki, F., Sumimoto, H
  • Journal Title: Biochem. J 396 Pages: 183-192
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Regulation of superoxide-producing NADPH oxidases in nonphagocytic cells. (2006)
  • Author(s): Takeya, R., Ueno, N., Sumimoto, H
  • Journal Title: Methods Enzymol 406 Pages: 456-468
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] NMR solution structure of the tandem SH3 domains of p47phax complexed with a p22phax-derived proline-rich nentide. (2006)
  • Author(s): Ogura, K., Nobuhisa, I., Yuzawa, S., Takeya, R., Torikai, S., Saikawa, K., Sumimoto, H., Inagaki, F
  • Journal Title: J. Biol. Chem 281 Pages: 3660-3668
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3. (2006)
  • Author(s): Izaki, T., Kamakura, S., Kohjima, M., Sumimoto, H
  • Journal Title: Biochem. Biophys. Res. Commun 341 Pages: 1001-1006
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Regulation of novel superoxide-producing NAD(P)H oxidases. (2006)
  • Author(s): Takeya, R., Sumimoto, H
  • Journal Title: Antioxid. Redox Signal 8 Pages: 1523-1532
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression and function of Noxoly, an alternative splicing form of the NADPH oxidase organizer 1. (2006)
  • Author(s): Takeya, R., Taura, M., Yamasaki, T., Naito, S., Sumimoto, H
  • Journal Title: FEBS J 273 Pages: 3663-3677
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phagocytosis-coupled activation of the superoxide-producing phagocyte oxidase, a member of the NADPH oxidase (Nox) family. (2006)
  • Author(s): Minakami, R., Sumimoto, H
  • Journal Title: Int. J. Hematol 84 Pages: 193-198
  • NAID: 10018303702
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of isoforms of NADPH oxidase components in rat pancreatic islets. (2006)
  • Author(s): Uchizono, U., Takeya, R., Iwase, M., Sasaki, N., Oku, M., Imoto, H., Iida, M., Sumimoto, H
  • Journal Title: Life Sci 80 Pages: 133-139
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Crystallization and preliminary crystallographic analysis of p40phox, a regulatory subunit of NADPH oxidase. (2006)
  • Author(s): Honbou, K., Yuzawa, S., Suzuki, N. N., Fujioka, Y., Sumimoto, H., Inagaki, F
  • Journal Title: Acta Crystallogr. F Struct. Biol. Cryst. Commun 62 Pages: 1018-1020
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] TRPC3 and TRPC6 are essential for angiotensin II-induced cardiac hypertrophy. (2006)
  • Author(s): Onohara, N., Nishida, M., Inoue, R., Kobayashi, H., Sumimoto, H., Sato, Y., Mori, Y., Nagao, T., Kurose, H
  • Journal Title: EMBO J 25 Pages: 5305-5316
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Increased expression of gp91phox homologues of NAD(P)H oxidase in the aortic media during chronic hypertension : involvement of renin-angiotensin system. (2006)
  • Author(s): Akasaki, T., Ohya, Y., Kuroda, J., Eto, K., Abe, I., Sumimoto, H., Iida, M
  • Journal Title: Hypertens. Res 29 Pages: 813-820
  • NAID: 10018561399
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Direct involvement of the small GTPase Rac in activation of the superoxide-producing NADPH oxidase Nox1. (2006)
  • Author(s): Miyano, K., Ueno, N., Takeya, R., Sumimoto, H.
  • Journal Title: J. Biol. Chem. 281 Pages: 21857-21868
• [Journal Article] Activation of the superoxide-producing phagocyte NADPH oxidase requires co-operation between the tandem SH3 domains of p47^<phox> in recognition of a polyproline type II helix and an adjacent α-helix of p22^<phox>. (2006)
  • Author(s): Nobuhisa, I., Takeya, R., Ogura, K., Ueno, N., Kohda, D., Inagaki, F., Sumimoto, H.
  • Journal Title: Biochem. J. 396 Pages: 183-192
• [Journal Article] Regulation of superoxide-producing NADPH oxidases in nonphagocytic cells. (2006)
  • Author(s): Takeya, R., Ueno, N., Sumimoto, H.
  • Journal Title: Methods Enzymol. 406 Pages: 456-468
• [Journal Article] NMR solution structure of the tandem SH3 domains of p47^<phox> complexed with a p22^<phox>-derived proline-rich peptide. (2006)
  • Author(s): Ogura, K., Nobuhisa, I., Yuzawa, S., Takeya, R., Torikai, S., Saikawa, K., Sumimoto, H., Inagaki, F.
  • Journal Title: J. Biol. Chem. 281 Pages: 3660-3668
• [Journal Article] Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3. (2006)
  • Author(s): Izaki, T., Kamakura, S., Kohjima, M., Sumimoto, H.
  • Journal Title: Biochem. Biophys. Res. Commun. 341 Pages: 1001-1006
• [Journal Article] Regulation of novel superoxide-producing NAD(P)H oxidases. (2006)
  • Author(s): Takeya, R., Sumimoto, H.
  • Journal Title: Antioxidant. Redox. Signal. 8 Pages: 1523-1532
• [Journal Article] Expression and function of Noxo1, an alternative splicing form of the NADPH oxidase organizer 1. (2006)
  • Author(s): Takeya, R., Taura, M., Yamasaki, T., Naito, S., Sumimoto, H.
  • Journal Title: FEBS J. 273 Pages: 3663-3677
• [Journal Article] Phagocytosis-coupled activation of the superoxide-producing phagocyte oxidase, a member of the NADPH oxidase (Nox) family. (2006)
  • Author(s): Minakami, R., Sumimoto, H.
  • Journal Title: Int. J. Hematol. 84 Pages: 193-198
  • NAID: 10018303702
• [Journal Article] Expression of isoforms of NADPH oxidase components in rat pancreatic islets. (2006)
  • Author(s): Uchizono, U., Takeya, R., Iwase, M., Sasaki, N., Oku, M., Imoto, H., Iida, M., Sumimoto, H.
  • Journal Title: Life Sci. 80 Pages: 133-139
• [Journal Article] Crystallization and preliminary crystallographic analysis of p40^<phox>, a regulatory subunit of NADPH oxidase. (2006)
  • Author(s): Honbou K, Yuzawa S, Suzuki NN, Fujioka Y, Sumimoto H, Inagaki F.
  • Journal Title: Acta Crystallograph Sect F Struct Biol Cryst Commun. 62 Pages: 1018-1020
• [Journal Article] TRPC3 and TRPC6 are essential for angiotensin II-induced cardiac hypertrophy. (2006)
  • Author(s): Onohara, N., Nishida, M., Inoue, R., Kobayashi, H., Sumimoto, H., Sato, Y., Mori, Y., Nagao, T., Kurose, H.
  • Journal Title: EMBO J. 25 Pages: 5305-5316
• [Journal Article] Increased expression of gp91^<phox> homologues of NAD(P)H oxidase in the aortic media during chronic hypertension; involvement of the rennin-angiotensin system. (2006)
  • Author(s): Akasaki, T., Ohya, Y., Kuroda, J., Eto, K., Abe, I., Sumimoto, H., Iida, M.
  • Journal Title: Hypertens. Res. 29 Pages: 813-820
• [Presentation] シンポジウム「生体内ラジカル/活性酸素種による生理応答機構と病態形成」 活性酸素生成型NADPHオキシダーゼの活性化の分子機構. (2008)
  • Author(s): 住本 英樹, 宮野 佳, 水上 令子.
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] シンポジウム 「NO/ROS in Cell Signaling」 Molecular mechanism for activation of the phagocyte NADPH oxidase (2007)
  • Author(s): Hideki Sumimoto
  • Organizer: 19th FAOBMB Seoul Conference
  • Place of Presentation: Seoul(Republic of Korea)
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Mechanism for activation of superoxide-producing NADPH oxidases (2007)
  • Author(s): Hideki Sumimoto
  • Organizer: The 4th Meeting of lnternational Redox Network
  • Place of Presentation: Jeju Island(Republic of Korea)
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Symposium "NO/ROS in Cell Signaling" Molecular mechanism for activation of the phagocyte NADPH oxidase (2007)
  • Author(s): Hideki, Sumimoto
  • Organizer: 19th FAOBMB Seoul Conference
  • Place of Presentation: Seoul(Republic of Korea)
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Mechanism for activation of superoxide-producing NADPH oxidases (2007)
  • Author(s): Hideki, Sumimoto
  • Organizer: The 4th Meeting of International Redox Network
  • Place of Presentation: Jeju Island(Republic of Korea)
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] シンポジウム 「NO/ROS in Cell Signaling」 Molecular mechanism for activation of the phagocyte NADPH oxidase. (2007)
  • Author(s): Hideki Sumimoto.
  • Organizer: 19^<th> FAOBMB Seoul Conference.
  • Place of Presentation: Seoul, Republic of Korea.
• [Presentation] Mechanism for activation of superoxide-producing NADPH oxidases. (2007)
  • Author(s): Hideki Sumimoto.
  • Organizer: The 4th Meeting of International Redox Network.
  • Place of Presentation: Jeju Island, Republic of Korea.
• [Presentation] ワークショップ 「酸化ストレスの評価方法と腎障害への応用」 活性酸素生成酵素NADPHオキシダーゼ(Nox)ファミリーの機能と調節機構. (2007)
  • Author(s): 住本 英樹.
  • Organizer: 第50回日本腎臓学会学術総会
  • Place of Presentation: 浜松
• [Presentation] ワークショップ「細胞骨格・細胞運動・細胞移動」 formin相同タンパク質Fhos1/FHOD1のリン酸化依存的活性化機構. (2007)
  • Author(s): 武谷 立, 住本 英樹
  • Organizer: 第40回日本発生生物学会 第50回日本細胞生物学会合同大会.
  • Place of Presentation: 福岡
• [Presentation] Molecular composition and regulation of Noxl-3.(invited speaker) (2006)
  • Author(s): Hideki Sumimoto
  • Organizer: Gordon Research Conferences on Nox-Family NADPH Oxidases, Les Diablerets(the Switzerland)
  • Place of Presentation: Les Diablerets (the Switzerland).
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Role of Rac in Regulation of Noxl-3.(invited speaker) (2006)
  • Author(s): Hideki Sumimoto
  • Organizer: The Phagosome and the Immune/lnflammatory Response(16th annual workshop of Massachusetts General Hospital's center of the study of inflammatory bowel disease)
  • Place of Presentation: Boston(USA)
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecular mechanism for activation of superoxide-producing NADPH oxidases.(invited speaker) (2006)
  • Author(s): Hideki Sumimoto
  • Organizer: The 50th Anniversary of Oxygenases:Advances and Reflections(The 14th Takeda Science Foundation Symposium on Bioscience)
  • Place of Presentation: Kyoto(Japan)
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecular composition and regulation of Nox1-3 (2006)
  • Author(s): Hideki, Sumimoto
  • Organizer: Gordon Research Conferences on Nox-Family NADPH Oxidases
  • Place of Presentation: Les Diablerets(the Switzerland)
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Role of Rac in Regulation of Nox1-3 (2006)
  • Author(s): Hideki, Sumimoto
  • Organizer: The Phagosome and the Immune/Inflammatory Response(16th annual workshop of Massachusetts General Hospital's center of the study of inflammatory bowel disease)
  • Place of Presentation: Boston(USA)
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular mechanism for activation of superoxide-producing NADPH oxidases (2006)
  • Author(s): Hideki, Sumimoto
  • Organizer: The 50th Anniversary of Oxygenases : Advances and Reflections(The 14th Takeda Science Foundation Symposium on Bioscience)
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 酸化ストレスの医学 (2008)
  • Author(s): 住本 英樹, 前原 優一, 宮野 佳
  • Publisher: 診断と治療社(印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 酸化ストレスの医学 (2008)
  • Author(s): 住本 英樹, 前原 優一, 宮野 佳.
  • Publisher: 診断と治療社(印刷中)
• [Book] 生体防御医学事典 (2007)
  • Author(s): 住本 英樹
  • Total Pages: 6
  • Publisher: 朝倉書店
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 生体防御医学事典 (2007)
  • Author(s): 住本 英樹.
  • Total Pages: 6
  • Publisher: 朝倉書店
• [Book] 生体防御医学辞典 (2007)
  • Author(s): 住本 英樹(共著者・共著者)
  • Publisher: 朝倉書店(印刷中)