Functional analysis of chondroitin sulfate E expressed in glioma

• Project/Area Number: 18390113
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Shinshu University
• Principal Investigator: NAKAYAMA Jun Shinshu University, 大学院・医学系研究科, 教授 (10221459)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥2,040,000); Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2007: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2006: ¥7,800,000 (Direct Cost: ¥7,800,000)
• Keywords: 分子病理 / 脳腫瘍 / 糖鎖遺伝子 / グリコサミノグリカン鎖 / ヘパリン結合性増殖因子 / RNA干渉 / in situ hybridization / 免疫組織化学 / 星細胞腫 / 細胞走触能 / 硫酸転移酵素 / 神経膠腫 / コンドロイチン硫酸 / 高速液体クロマトグラフィー / コンドロイチン硫酸E / 定量PCR

Research Abstract

高度に硫酸化されたグリコサミノグリカン鎖であるコンドロイチン硫酸E(CS-E)と、その生合成に係わるバアセチルガラクトサミン4硫酸6-0-硫酸転移酵素(Ga1NAc4S-6ST)のmRNAはヒト星細胞腫で高頻度に発現し、特に後者は予後不良因子であった。膠芽腫U251細胞においてGa1NAc4S-6STsiRNAの導入により、プレイオトロピンやミッドカインへの走触能が低下した。以上の結果より、CS-Eは星細胞腫の浸潤能と係わっていることが示唆された。

Research Products

• [Journal Article] Roles of gastric mucin-type o-glycans in the pathogenesis of Helicobacter pylori infection. (2009)
  • Author(s): Kobayashi M, Lee H, Nakayama.1, Fukuda M
  • Journal Title: Glycobiology 19 Pages: 453-461
  • Peer Reviewed
• [Journal Article] Carbohydrate-dependent defense mechanisms against Helicobacter pylori infection (2009)
  • Author(s): Kobayashi M, Lee H, Nakayama J, Fukuda M
  • Journal Title: Carr Burg Metab 10 Pages: 29-40
  • Peer Reviewed
• [Journal Article] Identification of mRNA splicing factor as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells (2009)
  • Author(s): Hatakeyama S, Sugihara K, Nakayama j, Akama TO, Wong SA, Kawashima H, Zhang J, Smith DF, Ohyama C, Fukuda M, Fukuda MN
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 3095-3100
  • Peer Reviewed
• [Journal Article] Genetic heterogeneity of EGFR mutation in pleomorphic carcinoma of the lung: Response to gefitinib and clinical outcome (2009)
  • Author(s): Ushiki A, Koizumi T, Kobayashi N, Kanda S, Yasuo M, Yamamoto H, Kubo K, Aoyagi D, Nakayama J
  • Journal Title: Jpn J Crin Oncol 39 Pages: 267-270
  • Peer Reviewed
• [Journal Article] Role of ASC in the mouse model of Helicobacter pylori infection (2009)
  • Author(s): Benoit BN, Kobayashi M, Kawakubo M, Takeoka M, Sane K, Zou J, Itano N, Tsutsui H, Neda T, Fukuda M, Nakayama J, Taniguchi S
  • Journal Title: J Histochem Cytochem 57 Pages: 327-338
  • Peer Reviewed
• [Journal Article] GleNAc6ST-l-mediated decoration of MAdCAM-l protein with L-selectin ligand carbohydrates directs disease activity of ulcerative colitis (2009)
  • Author(s): Kobayashi M, Hoshino H, Masumoto J, Fukushima M, Suzawa K, Kageyama S, Suzuki M, Ohtani H, Fukuda M, Nakayama J
  • Journal Title: Inflamm Bowel Dis 15 Pages: 697-706
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinase inhibits Gaq-induced at rial remodeling in transgenic mice (2009)
  • Author(s): Hirose M, Takeishi Y, Niizeki T, Shimojo H, Nakada T, Kubota I, Nakayama Mende U, Yamada M
  • Journal Title: Heart Rhythm 6 Pages: 78-84
  • Peer Reviewed
• [Journal Article] Identification of mRNA splicing factor as the endothelial receptor for carbohydrate-dependent lung colonization of canoer cells (2009)
  • Author(s): Hatakeyama, et al.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 3095-3100
  • Peer Reviewed
• [Journal Article] GlcNAc6ST-1-mediated decoration of MAdCAM-1 protein with L-selectin ligand carbohydrates directs disease activity of ulcerative colitis (2009)
  • Author(s): Kobayashi, et al.
  • Journal Title: Inflamm Bowel Dis 15 Pages: 697-706
  • Peer Reviewed
• [Journal Article] Trophinin : what embryo implantation teaches us about human cancer (2008)
  • Author(s): Fukuda MN, Sugihara K, Nakayama J
  • Journal Title: Cancer Biol Ther 7 Pages: 1165-1170
  • Peer Reviewed
• [Journal Article] Differential distribution of synGAPal and synGAPP isoforms in rat neurons (2008)
  • Author(s): Moon IS, Sakagami H, Nakayama J, Suzuki T
  • Journal Title: Brain Res 1241 Pages: 62-75
  • Peer Reviewed
• [Journal Article] Sulfation patterns of keratan sulphate in different macular corneal dystrophy immunophenotypes using three different probes (2008)
  • Author(s): Saito T, Nishida K, Nakayama J, Akama TO, Fukuda MN, Watanabe K, Quantock AI, Maeda N, Watanabe H, Tana Y
  • Journal Title: BrJOphthalmol 92 Pages: 1434-1436
  • Peer Reviewed
• [Journal Article] αl,4GlcNAc-cappedmucin-type O-glycan inhibits cholesterol α-glucosyltransferase from Helicobacter pylori and suppresses H. pylori growth (2008)
  • Author(s): Lee H, Wang P, Hoshino H, Ita Y, Kobayashi M, Nakayama J, Seeberger PH, Fukuda M
  • Journal Title: Glycobiology 18 Pages: 549-558
  • Peer Reviewed
• [Journal Article] Critical role of bone marrow ASC, an inflammasome adaptor molecule, in neointimal formation after vascular injury in mice (2008)
  • Author(s): Yajima N, Takahashi M, Morimoto H, Shiba Y, Takahashi Y, Masumoto J, Ise H, Sagara J, Nakayama J, Taniguchi S, Ikeda U
  • Journal Title: Circulation 117 Pages: 3079-3087
  • Peer Reviewed
• [Journal Article] NADPH oxidase 1 plays a critical mediating role in oncogenic Ras-induced vascular endothelial growth factor expression (2008)
  • Author(s): Komatsu D, Kate M, Nakayama J, Miyagawa S, Kamata T
  • Journal Title: Ongogene 27 Pages: 4724-4732
  • Peer Reviewed
• [Journal Article] Enhancement of human sperm motility by trophinin binding peptide (2008)
  • Author(s): Hatakeyama S, Sugihara K, Lee SH, Nadano D, Nakayama J, Ohyama C, Fukuda MN
  • Journal Title: J Ural 180 Pages: 767-771
  • Peer Reviewed
• [Journal Article] Critical roles of VEGF-C-VEGF receptor 3 in reconnection of the collecting lymph vessels in mice (2008)
  • Author(s): Ikomi F, Kawai Y, Nakayama J, Ogiwara N, Sasaki K, Mizuno R, Ohhashi T
  • Journal Title: Microcirculation 15 Pages: 591-603
  • Peer Reviewed
• [Journal Article] Induction of trophinin in human endometrial surface epithelia by CGβ and IL-lβ (2008)
  • Author(s): Sugihara K, Kabir-Salmani M, Byrne J, Wolf DP, Lessey B, Iwashita M, Aoki D, Nakayama J, Fukuda MN
  • Journal Title: FEBS Left 582 Pages: 197-202
  • Peer Reviewed
• [Journal Article] Critical role of bone marrow ASC, an in flammasome adaptor molecule, in neointimal formation after vascular injury in mice (2008)
  • Author(s): Yajima, et al.
  • Journal Title: Circulation 117 Pages: 3079-3087
  • Peer Reviewed
• [Journal Article] Analysis of immunohistochemical expression of p53 and the proliferation marker Ki-67 antigen in skull base chordomas : relationships between their expression and prognosis (2007)
  • Author(s): Sakai K, Hongo K, Tanaka Y, Nakayama J
  • Journal Title: Brain Tumor Pathol 24 Pages: 57-62
  • Peer Reviewed
• [Journal Article] Usefulness of monoclonal antibody HIK1083 specific for gastric o-glyean in differentiating cutaneous metastasis of gastric cancer from primary sweat gland carcinoma (2007)
  • Author(s): Iijima M, Nakayama J, Nishizawa T, Ishida A, Ishii K, Ota H, Katsuyama T, Saida T
  • Journal Title: Am J Dermatopathol 29 Pages: 452-456
  • Peer Reviewed
• [Journal Article] Tranilast suppresses the disease development of the adjuvant- and streptococcal cell wall-induced arthritis in rats (2007)
  • Author(s): Nagate T, Tamura T, Sato F, Kuroda J, Nakayama J, Shibata N
  • Journal Title: J Pharmacol Sci 105 Pages: 48-56
  • Peer Reviewed
• [Journal Article] Prognositic significance of the immunohistochemical staining of cleaved-caspase-3, an activated-form of caspase-3, in gliomas (2007)
  • Author(s): Kobayashi T, Masumoto J, Tada T, Nomiyama T, Hongo K, Nakayama J
  • Journal Title: Crin Cancer Res 13 Pages: 3868-3874
  • Peer Reviewed
• [Journal Article] RNA interference-mediated knock-down of transient receptor potential vanilloid 1 prevents forepaw inflammatory hyperalgesia in rat (2007)
  • Author(s): Kasama S, Kawakubo M, Suzuki T, Nishizawa T, Ishida A, Nakayama J
  • Journal Title: Eur J Neurosci 25 Pages: 2956-2963
  • Peer Reviewed
• [Journal Article] The role of trophinin, an abhesion molecule unique to human trophoblasts, in progression of colorectal cancer (2007)
  • Author(s): Harada O, Suga T, Suzuki T, Nakamoto K, Kobayashi M, Nomiyama T, Nadano D, Ohyama C, Fukuda MN, Nakayama J
  • Journal Title: Int J Cancer 121 Pages: 1072-1078
  • Peer Reviewed
• [Journal Article] Preferential induction of peripheral lymph node addressin on high endothelial venule-like vessels in the active phase of ulcerative colitis (2007)
  • Author(s): Suzawa K, Kobayashi M, Sakai Y, Hoshino H, Watanabe M, Harada O, Ohtani H, Fukuda M, Nakayama J
  • Journal Title: Am J Gastroenterol 102 Pages: 1499-1509
  • Peer Reviewed
• [Journal Article] The utility of formalin-fixed and paraffin-embedded tissue blocks for quantitative analysis of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase mRNA expressed by colorectal cancer cells (2007)
  • Author(s): Ito Y, Watanabe M, Nishizawa T, Omachi T, Kobayashi T, Habuchi O, Nakayama J
  • Journal Title: Acts Histochem Cytochem 40 Pages: 53-59
  • Peer Reviewed
• [Journal Article] Bacteriophage biopanning in human tumour biopsies to identify cancer-specific targeting ligands (2007)
  • Author(s): Maruta F, Akita N, Nakayama J, Miyagawa S, Ismail T, Rowlands DC, Kerr DJ, Fisher KD, Seymour LW, Parker AL
  • Journal Title: J Drug Target 15 Pages: 311-319
  • Peer Reviewed
• [Journal Article] Trophoblast cell activation by trophinin ligation : Possible role in human embryo implantation (2007)
  • Author(s): Sugihara K, Sugiyama D, Byrne J, Wolf DP, Lowitz KP, Kobayashi Y, Kabir-Salmani M, Nadano D, Aoki D, Nozawa S, Nakayama J, Mustelin T, Ruoslahti E, Yamaguchi N, Fukuda MN
  • Journal Title: Proc Natl Acad Sci USA 104 Pages: 3799-3804
  • Peer Reviewed
• [Journal Article] A distinctive set of genes is up-regulated during the inflammation-carcinoma sequence in mouse stoma' infected by Helicobacter fells (2007)
  • Author(s): Kobayashi M, Lee H, Schaffer L, Gilmartin TJ, Head SR, Takaishi S, Wang TC, Nakayama J, Fukuda M
  • Journal Title: J histochem Cytochem 55 Pages: 263-274
  • Peer Reviewed
• [Journal Article] Esophageal gland duct adenoma : Immunohistochemical comparison with the normal esophageal gland and ultrastractural analysis (2007)
  • Author(s): Harada O, Ota H, Katsuyama T, Hidaka E, Ishizaka K, Nakayama J
  • Journal Title: Am J Surg Pathol 31 Pages: 469-475
  • Peer Reviewed
• [Journal Article] The utility of formalin-fixed and paraffin-embedded tissue blocks for quantitative analysis of N-acetylgalactosamine 4-sulfate 6-0-sulfotransferase mRNA expressed by colorectal cancer cells (2007)
  • Author(s): Ito, et. al.
  • Journal Title: Acta Histochemica et Cytochemica 40 Pages: 53-59
  • Peer Reviewed
• [Journal Article] Prognositic significance of the immunohistochemical staining of cleaved-caspase-3, an activated-form of caspase-3, in gliomas (2007)
  • Author(s): Kobayashi, et. al.
  • Journal Title: Clinical Cancer Research 13 Pages: 3868-3874
  • Peer Reviewed
• [Journal Article] The utility of formalin-fixed and paraffin-embedded tissue blocks for quantitative analysis of N-acetylgalactosamine 4-sulfate 6-0-sulfotransferase mRNA expressed by colorectal cancer cells (2007)
  • Author(s): Ito et al.
  • Journal Title: Acta Histochemica et Cytochemica (印刷中)
• [Journal Article] Prognositic significance of the immunohistochemical staining of cleaved-caspase-3, an activated-form of caspase-3, in gliomas (2007)
  • Author(s): Kobayashi et al.
  • Journal Title: Clinical Cancer Research (印刷中)
• [Journal Article] Expression cloning of cholesterol a-glucosyltransferase, a unique enzyme that can be inhibited by natural antibiotic gastric mucin O-glyeans, from Helicobacter pylori (2006)
  • Author(s): Lee H, Kobayashi M, Wang P, Nakayama J, Seeberger PH, Fukuda M
  • Journal Title: Biochem Biophys Res Commun 349 Pages: 1235-1241
  • Peer Reviewed
• [Journal Article] Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-0 sulfotransferase (2006)
  • Author(s): Hayashida Y, Akama TO, Beecher N, Lewis P, Young RD, Meek KM, Kerr B, Hughes CE, Caterson B, Tanigami A, Nakayama J, Fukada MN, Tano Y, Nishida K, Quantock AJ
  • Journal Title: Proc Natl Acad Sci USA 103 Pages: 13333-13338
  • Peer Reviewed
• [Journal Article] Interleukin-1βsuppression in Nod2-defect Blau syndrome
  • Author(s): Masumoto J, Yamazaki T, Ohta K, Nakayama J, Agematsu K
  • Journal Title: Arthritis Rheum in press
  • Peer Reviewed
• [Journal Article] Therapeutic and preventive effects of methotrexate on zymosan-induced arthritis developed on SKG mice
  • Author(s): Nagate T, Kawai J, Nakayama J
  • Journal Title: J Vet Med Sci in press
  • Peer Reviewed
• [Presentation] ピロリ菌感染胃粘膜におけるαGlcNAc含有O-グリカンの役割 (2008)
  • Author(s): 中山淳
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17
• [Presentation] ピロリ菌感染胃粘膜におけるα GlcNAc含有0-グリカンの役割 (2008)
  • Author(s): 中山淳
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢市
  • Year and Date: 2008-05-17
• [Presentation] Preferential glycosylation of MAdCAM-1on high endothelial venule-like vessels in ulcerative colitis (2008)
  • Author(s): 小林基弘, 福島万奈, 大谷明夫, 中山淳
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-16
• [Presentation] Prognostic significance of immunohistochemical staining of cleaved-caspase 3, an activated-form ofcaspase-3, in glimas (2007)
  • Author(s): Masumoto J, Kobayashi T, Tada T, Hongo K, Nakayama J
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-05
• [Presentation] Prognostic significance of immunohistochemical staining of cleaved-caspase-3, an activated-form of caspase-3, in gliomas (2007)
  • Author(s): 増本純也、他
  • Organizer: 第66回 日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-10-05
• [Presentation] Application of immunohistochemistry for study of ASC-related molecules in diseases (2007)
  • Author(s): Masumoto J, Nakayama J
  • Organizer: 第48回日本組織細胞化学会総会
  • Place of Presentation: 甲府
  • Year and Date: 2007-09-29
• [Presentation] Preferential induction of PNAd on HEV-like vessels in the active phase of ulcerative colitis (2007)
  • Author(s): 小林基弘, 大谷明夫, 中山淳
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-15
• [Book] Experimental Glycoscience (2008)
  • Author(s): Nakayama J, Furukawa K
  • Publisher: Springer-Verlag, Tokyo
• [Book] Experimental Glycoscience (2008)
  • Author(s): Nakayama J
  • Publisher: Springer-Verlag, Tokyo
• [Book] Comprehensive Glycoscience (2007)
  • Author(s): Kobayashi M, Fukuda M, Nakayama J
  • Publisher: Elsevier, Oxford
• [Remarks]
  • URL: http://www.shinshu-u.ac.jp/faculty/medicine/chair/i-lbyori/defauhimhtm
• [Remarks]
  • URL: http://www.shinshu-u.ac.jp/faculty/medicine/chair/i-lbyori/default.htm