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Is the superantigenic activity required for the emetic activity of staphylococcal enterotoxin A

Research Project

Project/Area Number 18390132
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionHirosaki University

Principal Investigator

NAKANE Akio  Hirosaki University, Hirosaki University Graduate School of Medicine, Professor (30164239)

Co-Investigator(Kenkyū-buntansha) HU Ding-Liang  Hirosaki University, Graduate School of Medicine, Associate Professor (10333733)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥17,150,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2007: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2006: ¥6,100,000 (Direct Cost: ¥6,100,000)
KeywordsEnterotoxin / Staphylococcus aureus / Superantigen / Emesis / Suncus / Cytokine / 嘔吐
Research Abstract

Staphylococcal enterotoxin A (SEA) is an extracellular protein produced by Staphylococcus aureus. SEA shows emetic activity and superantigenic activity. The mechanism of emetic activity of SEA has been little known. In the present study, we demonstrated the following results:
Mutation was carried out at sites of T-cell receptor- and MHC class II molecule-binding regions on SEA by site-directed mutagenesis. We prepared 13 mutant SEA (mSEA) proteins. We examined superantigenic activities of mSEAs in human peripheral blood leukocytes (HPBL) and spleen cells of house musk shrews (Suncus murinus). Moreover, we compared emesis-inducing activities among these mSEAs by intraperitoneal injection. All mSEAs reacted with anti-SEA antibody. Superantigenic activities of mSEAs including N25G, F47G, C96G, C106A, V174G and D227A were deficient because cell proliferation and induction of IL-2, IFN-γ and TNF-α in HPBL were reduced in these mutant proteins. Activities of cell proliferation to spleen cell of house musk shew were reduced in mSEA including F47G, F47S, C96G, C106A, V174G and D227A. Emesis-inducing activities to house musk shew were reduced in F47G, F47S, H61D, H187A and D227A. These results suggest that sites of F47 and D227 on a SEA molecule are essential for both superantigenic and emesis-inducing activities and of H61, H187 and H225 for emesis-inducing activity and that active sites for superantigenic and emesis-inducing activities may be different.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (28 results)

All 2008 2007 2006 Other

All Journal Article (15 results) (of which Peer Reviewed: 9 results) Presentation (11 results) Remarks (2 results)

  • [Journal Article] Intranasal immunization of mutant toxic shock syndrome toxin 1 elicit systemic and mucosal immune response against Staphylococcus aureus infection.2008

    • Author(s)
      Narita, K, and Nakane, A , et. al.
    • Journal Title

      FEMS Immunology and Medical Microbiology 52

      Pages: 389-396

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Protective effect of intranasal vaccination with nontoxic mutant toxic shock syndrome toxin 1 against systemic and local staphylococcus aureus infection.2008

    • Author(s)
      Narita, K and Nakane, A , et. al.
    • Journal Title

      Hirosaki Medical. Journal 59

      Pages: 71-82

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Intranasal immunization of mutant toxic shock syndrome toxin 1 elicits systemic and mucosal immune response against Staphylococcus aureus infection2008

    • Author(s)
      Narita K, Hu D-L, Tsuji T, Nakane A.
    • Journal Title

      FEMS Immunology and Medical Microbioology 52

      Pages: 389-396

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Protective effect of intranasal vaccination with nontoxic mutant toxic shock syndrome toxin 1 against Staphylococcus aureus infections2008

    • Author(s)
      Narita K, Hu D-L, Tsuji T, Nakane A.
    • Journal Title

      Hirosaki Medical Journal 59

      Pages: 71-82

    • NAID

      110006622361

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Intranasal immunization of mutant toxic shock syndrome toxin 1 elicit systemic and mucosal immune response against Staphylococcus aureus infection.2008

    • Author(s)
      Narita, K, and Nakane, A, et. al.
    • Journal Title

      FEMS Immunology and Medical Microbiology 52

      Pages: 389-396

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protective effect of intranasal vaccination with nontoxic mutant toxic shock syndrome toxin 1 against systemic and local Staphylococcus aureus infection.2008

    • Author(s)
      Narita, K, and Nakane, A, et. al.
    • Journal Title

      Hirosaki Medical. Journal 59

      Pages: 71-82

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Staphylococcal enterotoxin induces emesis through increasing serotonin release in intestine and it is down-regulated by cannabinoid receptor 1.2007

    • Author(s)
      Hu, D-L and Nakane, A et a1.
    • Journal Title

      Cellular Microbiology 9

      Pages: 2267-2277

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Pollution of enteropathogenic bacteria in meats and egg in Japan and China.2007

    • Author(s)
      Hu, D-L and Nakane, A , et. al.
    • Journal Title

      Hirosaki Medical Journal 59

      Pages: 1-6

    • NAID

      110006573840

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Staphylococcal enterotoxin induces emesis through increasing serotonin release in intestine and it is downregulated by cannabinoid receptor 12007

    • Author(s)
      Hu D-L, Zhu G, Mori F, Omoe K, Okada M, Wakabayashi K, Kaneko S, Shinagawa K, Nakane A.
    • Journal Title

      Cellular Microbiology 9

      Pages: 2267-2277

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Pollution of enteropathogenic bacteria in meats and egg in Japan and China2007

    • Author(s)
      Hu D-L, Qian A-D, San X-F, Narita K, Sashinam H, Osanai K, Abe Y, Hiraga H, Kudo K, Liu Y, Nakane A.
    • Journal Title

      Hirosaki Medical Journal 59

      Pages: 1-6

    • NAID

      110006573840

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Staphylococcal enterotoxin induces emesis through increasing serotonin releasein intestine and it is down-regulated by cannabinoid receptor 1.2007

    • Author(s)
      Hu, D-L and Nakane, A, et. al.
    • Journal Title

      Cellular Microbiology 9

      Pages: 2267-2277

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pollution of enteropathogenic bacteria in meats and egg in Japan and China.2007

    • Author(s)
      Hu, D-L and Nakane, A, et. al.
    • Journal Title

      Hirosaki Medical Journal 59

      Pages: 1-7

    • NAID

      110006573840

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intranasal vaccination with a double mutant of staphylococcal enterotox in C provides protection against Staphylococcus aureus infection2006

    • Author(s)
      Hu, D-L, and Nakane, A , et. al.
    • Journal Title

      Microbes and Infection 8

      Pages: 2841-2848

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Intranasal vaccination with a double mutant of staphylococcal enterotoxin C provides protection against Staphylococcus aureus infection2006

    • Author(s)
      Hu D-L, Omoe K, Narita K, Cui J-C, Shinagawa K, Nakane A.
    • Journal Title

      Microbes and Infection 8

      Pages: 2841-2848

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Intranasal vaccination with a double mutant of staphylococcal enterotoxin C provides protection against Staphylococcus aureus infection2006

    • Author(s)
      Hu, D-L, Nakane A, et al.
    • Journal Title

      Microbes and Infection 8(14-15)

      Pages: 2841-2848

    • Related Report
      2006 Annual Research Report
  • [Presentation] ブドウ球菌エンテロトキシンの嘔吐誘導活性とスーパー抗原活性の関連性2008

    • Author(s)
      胡 東良, 中根明夫, ら
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都
    • Year and Date
      2008-03-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 黄色ブドウ球菌接着因子による感染予防効果2008

    • Author(s)
      成田浩司, 中根明夫, ら
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都
    • Year and Date
      2008-03-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Relationship between emesis-inducing activity and Superantigenic activity of staphylococcal enterotoxin2008

    • Author(s)
      Hu D-L, ed. al.
    • Organizer
      The 81th Annula Meeting of Japanese Society for Bacteriology
    • Place of Presentation
      Kyoto
    • Year and Date
      2008-03-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The preventive effect of staphylococcal adhesion molecules against Staphylococcus aureus infection2008

    • Author(s)
      Narita K, ed. al.
    • Organizer
      The 81th Annula Meeting of Japanese Society for Bacteriology
    • Place of Presentation
      Kyoto
    • Year and Date
      2008-03-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 黄色ブドウ球菌局所感染および全身感染に対する無毒変異TSST-1経鼻粘膜ワクチンの防御効果2007

    • Author(s)
      成田浩司, 中根明夫, ら
    • Organizer
      第37回日本免疫学会総会
    • Place of Presentation
      東京
    • Year and Date
      2007-11-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The protective effect of intranasal vaccination with nontoxic mutant TSST-1 on systemic and local Staphylococcus aureus infections2007

    • Author(s)
      Narita K, ed. al.
    • Organizer
      The 37^<th> Annual Meeting of Japanese Society for Immunology
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-11-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 黄色ブドウ球菌局所感染および全身感染に対する無毒変異TSST-1経鼻粘膜ワクチン防御効果2007

    • Author(s)
      成田浩司, 中根明夫, ら
    • Organizer
      第37回日本免疫学会総会
    • Place of Presentation
      東京
    • Year and Date
      2007-11-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] ブドウ球菌エンテロトキシンの嘔吐誘導機序2007

    • Author(s)
      胡 東良, 中根明夫, ら
    • Organizer
      第54回日本毒素シンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2007-09-06
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Mechanism of emesis of staphylococcal enterotoxin2007

    • Author(s)
      Hu D-L, ed. al.
    • Organizer
      The 54^<th> Toxin Symposium
    • Place of Presentation
      Osaka
    • Year and Date
      2007-09-06
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 無毒変異TSST-1経鼻粘膜ワクチンによる黄色ブドウ球菌感染防御効果2007

    • Author(s)
      成田浩司, 中根明夫, ら
    • Organizer
      第72回日本インターフェロン・サイトカイン学会
    • Place of Presentation
      京都
    • Year and Date
      2007-07-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] The protective effect of intranasal vaccination with nontoxic mutant TSST-1 on Staphylococcus aureus infection2007

    • Author(s)
      Narita K, ed. al.
    • Organizer
      The 72th Japanese Society for Interferon and Cytokine Research
    • Place of Presentation
      Kyoto
    • Year and Date
      2007-07-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.bact.hirosaki-u.ac.jp/

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.bact.hirosaki-u.ac.jp/

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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