Is the superantigenic activity required for the emetic activity of staphylococcal enterotoxin A
| Project/Area Number |
18390132
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Hirosaki University |
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Principal Investigator |
NAKANE Akio Hirosaki University, Hirosaki University Graduate School of Medicine, Professor (30164239)
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| Co-Investigator(Kenkyū-buntansha) |
HU Ding-Liang Hirosaki University, Graduate School of Medicine, Associate Professor (10333733)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥17,150,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2007: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2006: ¥6,100,000 (Direct Cost: ¥6,100,000)
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| Keywords | Enterotoxin / Staphylococcus aureus / Superantigen / Emesis / Suncus / Cytokine / 嘔吐 |
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| Research Abstract |
Staphylococcal enterotoxin A (SEA) is an extracellular protein produced by Staphylococcus aureus. SEA shows emetic activity and superantigenic activity. The mechanism of emetic activity of SEA has been little known. In the present study, we demonstrated the following results:
Mutation was carried out at sites of T-cell receptor- and MHC class II molecule-binding regions on SEA by site-directed mutagenesis. We prepared 13 mutant SEA (mSEA) proteins. We examined superantigenic activities of mSEAs in human peripheral blood leukocytes (HPBL) and spleen cells of house musk shrews (Suncus murinus). Moreover, we compared emesis-inducing activities among these mSEAs by intraperitoneal injection. All mSEAs reacted with anti-SEA antibody. Superantigenic activities of mSEAs including N25G, F47G, C96G, C106A, V174G and D227A were deficient because cell proliferation and induction of IL-2, IFN-γ and TNF-α in HPBL were reduced in these mutant proteins. Activities of cell proliferation to spleen cell of house musk shew were reduced in mSEA including F47G, F47S, C96G, C106A, V174G and D227A. Emesis-inducing activities to house musk shew were reduced in F47G, F47S, H61D, H187A and D227A. These results suggest that sites of F47 and D227 on a SEA molecule are essential for both superantigenic and emesis-inducing activities and of H61, H187 and H225 for emesis-inducing activity and that active sites for superantigenic and emesis-inducing activities may be different.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] Pollution of enteropathogenic bacteria in meats and egg in Japan and China2007
Author(s)
Hu D-L, Qian A-D, San X-F, Narita K, Sashinam H, Osanai K, Abe Y, Hiraga H, Kudo K, Liu Y, Nakane A.
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Journal Title
Hirosaki Medical Journal 59
Pages: 1-6
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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