Research Project
Grant-in-Aid for Scientific Research (B)
Resistance to a human natural immunomechanism by Pseudomonas aeruginosa and Serracia marcescens that were the Gram-negative opportunistic bacteria, and the following result was obtained.1) Pseudomonas aeruginosa: When the appearance of elastase synthesized gene (lasB) and Rhamunolipid synthesized gene (rhlA) with the acyl-homoserine lactones (HSLs) was compared, 3-oxo-C12-HSL was the highest. The deficiency of mexB has increased the appearance of lasB that responds to 3-oxo-Cn-HSL of C8 to C14 at a different rate. Each manifestation of a gene, the pyocyanine, and the change in the amount of the elastase of the production life were corresponding. The xenobiotic efflux system MexAB-OprM of P. aeruginosa not only took part in the multidrug-resistance but also selecting acyl-HSLs that had to respond, controlling quorum sensing system, and contributing to pathogenic expression to the human.2) Serracia marcescens. Tn insertion mutant library of about 8,000 clones was constructed, and the serum susceptibility of all clones was examined. Then, 79 serum susceptible mutants were obtained. When Tn inserted genes in the these serum susceptible mutants was analyzed, it was shown that Tn insertion in genes encoding to core region of the lipopolysaccharide and concerning the synthesis of O antigen increased the serum susceptibility. It was suggested that the conformational change on the cell surface plays an important role to the serum resistance of S. marcescens against human complements.
All 2008 2007 2006 Other
All Journal Article (20 results) (of which Peer Reviewed: 5 results) Presentation (54 results) Book (3 results) Remarks (2 results)
Microbiol.Immunol. 51
Pages: 271-277
J.Antibiot. 60
Pages: 129-135
Microbiol. Immunol. 51
J. Antibiot. 60
Microbiol.Immunol 51
J.Antibiot 60
日本臨床 65
Pages: 457-462
感契と抗菌薬 11
Pages: 24-29
呼吸器科 12
Pages: 89-94
医学のあゆみ 221
Pages: 511-515
日本臨牀 65(増刊2)
科学療法の領域 23
Pages: 29-34
Microbiol.Immunol. 50
Pages: 395-401
Microbiol. Immunol 50
Pathophysiological and biochemical analyses target validation for drug therapy(Ed. Takeshi Nishino and Koji Takeuchi)(Research Signpost)
Pages: 125-135
Pages: 937-950
小児内科 38
Pages: 1127-1132
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