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Escape mechanisms of HIV-1 from HIV-1-specific CTLs

Research Project

Project/Area Number 18390141
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionKumamoto University

Principal Investigator

TAKIGUCHI Masafumi  Kumamoto University, Center for AIDS Research, Professor (00183450)

Co-Investigator(Kenkyū-buntansha) OKA Shinichi  Kumamoto University, International Medical Center of Japan, ACC Director (20194326)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,310,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2007: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2006: ¥7,600,000 (Direct Cost: ¥7,600,000)
KeywordsVirus / Pathogenesis / Control of infection / HIV-1 / T Cell
Research Abstract

It is well known that HLA-B^*57/58, -B^*27, and -B*^51 alleles are associated with slow disease progression to AIDS, but the role of cytotoxic T lymphocytes(CTLs)restricted by these alleles in long-term control of HIV-1 still remains unknown. We investigated HIV-1-specific CTLs in long-term non-progressing(LTNP)and slow-progressing(slow progressors)hemophiliacs carrying HLA-B^*5101. Both Pol283-8-specific and Pol743-9-specific CTLs, which have a strong ability to suppress HIV-1 replication in vitro, were detected in LTNPs whereas only Pol743-9-specific CTLs were found in the slow progressors. The LTNPs had HIV-1 possessing wild-type sequence in these epitopes or a mutation reducing the ability of the specific CTLs to suppress HIV-1 replication and viral fitness. In contrast, the slow progressors and progressors had HIV-1 with escape mutations that did not influence viral fitness. Longitudinal analysis of a hemophiliac confirmed the association between the appearance of a Pol283-8 escape mutant and a high viral load. The induction of HIV-1-specific CTLs with a strong ability to suppress HIV-1 replication and the evolution to a mutant with reduced viral fitness and T cell recognition were found to be necessary for the control of HIV-1 for approximately 25 years.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (32 results)

All 2008 2007 2006 Other

All Journal Article (11 results) (of which Peer Reviewed: 4 results) Presentation (19 results) Remarks (2 results)

  • [Journal Article] Different Ability of Escape Mutant-Specific Cytotoxic T Cells to Suppress Replication of Escape Mutant and Wild-type HIV-1 in New Hosts2008

    • Author(s)
      Mamoru Fujiwara
    • Journal Title

      Journal of Virology 82

      Pages: 138-147

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Different immunodominance of HIV-1-specific CTL epitopes among3subtypes of HLA-A*26associated with slow progression to AIDS2008

    • Author(s)
      Yuka Kawashima
    • Journal Title

      Biochem.Biophys.Res.Commun. 366

      Pages: 612-616

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Different Ability of Escape Mutant-Specific Cytotoxic T Cells to Suppress Replication of Escape Mutant and Wild-type HIV-1 in New Hosts2008

    • Author(s)
      Mamoru, Fujiwara
    • Journal Title

      Journal of Virology 82

      Pages: 138-147

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Different immunodominance of HIV-1-specific CTL epitopes among 3 subtypes of HLA-A*26 associated with slow progression to AIDS2008

    • Author(s)
      Yuka, Kawashima
    • Journal Title

      Biochem. Biophys. Res. Commun 366

      Pages: 612-616

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Different immunodominance of HIV-1-specific CTL epitopes among 3 subtypes of HLA-A*26 associated with slow progression to AIDS2008

    • Author(s)
      Yuka Kawashima
    • Journal Title

      Biochem.Biophys.Res.Commun 366

      Pages: 612-616

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HIV-1-Specific CTLs Effectively Suppress Replication of HIV-1 in HIV-1-infected Macrophages2007

    • Author(s)
      Mamoru Fujiwara
    • Journal Title

      Blood 109

      Pages: 4832-4838

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] HIV-1-Specific CTLs Effectively Suppress Replication of HIV- 1 in HIV- 1 -infected Macrophages2007

    • Author(s)
      Mamoru, Fujiwara
    • Journal Title

      Blood 109

      Pages: 4832-4838

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Involvement of multiple epitope-specific cytotoxic T lymphocyte responses in vaccine-based control of simian immunodeficiency virus replication in rhesus macaques.2006

    • Author(s)
      Miki Kawada, et al.
    • Journal Title

      J.Virol. 80

      Pages: 1949-1958

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Increased Lavel and Longevity of Protective Immune Responses Induced by DNA Vaccine Expressing the HIV-1 Env Glycoprotein when Combined with of IL-21 and IL-15 Gene Delivery2006

    • Author(s)
      Elizabeth Bolesta, et al.
    • Journal Title

      J.Immunol. 177

      Pages: 177-191

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Altering effects of antigenic variations in HIV-1 on antiviral effectiveness of HIV-specific CTLs.

    • Author(s)
      Takamasa Ueno, et al.
    • Journal Title

      J.Immunol. in press

    • Related Report
      2006 Annual Research Report
  • [Journal Article] HIV-1-Specific CTLs Effectively Suppress Replication of HIV-1 in HIV-1-infected Macrophages,

    • Author(s)
      Mamoru Fujiwara, et al.
    • Journal Title

      Blood in press

    • Related Report
      2006 Annual Research Report
  • [Presentation] Accumulation of escape mutants by Pol283-8-specific cytotoxic Tlymphocytes in2different HLA class I restriction2007

    • Author(s)
      Yuichi Yagita
    • Organizer
      4th International AIDS Society Conference2007
    • Place of Presentation
      Sydney,Australia
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Selection of virla mutations by Pol283-8-specific CTLs in2different HLA class I restrictions2007

    • Author(s)
      Yuichi Yagita
    • Organizer
      8th AIDS Seminar in KUMAMOTO
    • Place of Presentation
      Kumamoto,Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Different Ability of Escape Mutant-Specific Cytotoxic T Cells to Suppress Replication of Escape Mutant and Wild-type HIV-1 in New Hosts2007

    • Author(s)
      Mamoru Fujiwara
    • Organizer
      8th AIDS Seminar in KUMAMOTO
    • Place of Presentation
      Kumamaoto,Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Control of HIV-1 rephcation by HIV-1-specific CTL i HLA-B*51+long-term non progressors2007

    • Author(s)
      Yuka Kawashima
    • Organizer
      8th AIDS Seminar in KUMAMOTO
    • Place of Presentation
      Kumamoto,Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Control of HIV-1 by HIV-1-specific CTL in vitro and in vivo2007

    • Author(s)
      Masafumi Takiguchi
    • Organizer
      Center for AIDS Research, Kumamoto University, 10th Anniversary Symposium
    • Place of Presentation
      Kumamoto,Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Long-term Control of HIV-1 by HIV-1-Specific CTLs and Appearance of Lower Fitness Virus in HLA-B*51+Long term non-progressors2007

    • Author(s)
      Yuka Kawashima
    • Organizer
      第37回日本免疫学会学術集会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] HLA-A*2402拘束性HIV-1 Nef由来免疫ドミナントエピトープ領域のエスケープ変異に対する細胞傷害性T細胞の応答2007

    • Author(s)
      藤原 守
    • Organizer
      第55回日本ウイルス学会学術集会
    • Place of Presentation
      札幌
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Accumulation of escape mutants by Pol283-8-specific cytotoxic T lymphocytes in 2 different HLA class I restriction2007

    • Author(s)
      Yuichi, Yagita
    • Organizer
      4th International AIDS Society Conference
    • Place of Presentation
      Sydney, Australia
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Selection of viral mutations by Pol283-8-specific CTLs in 2 different HLA class I restrictions2007

    • Author(s)
      Yuichi, Yagita
    • Organizer
      8th AIDS Seminar in KUMAMOTO
    • Place of Presentation
      Kumamoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Different Ability of Escape Mutant-Specific Cytotoxic T Cells to Suppress Replication of Escape Mutant and Wild-type HIV-1 in New Hosts2007

    • Author(s)
      Mamoru, Fujiwara
    • Organizer
      8th AIDS Seminar in KUMAMOTO
    • Place of Presentation
      Kumamoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Control of HIV-1 replication by HIV-1-specific CTL in HLA-B*51+ long-term non progressors2007

    • Author(s)
      Yuka, Kawashima
    • Organizer
      8th AIDS Seminar in KUMAMOTO
    • Place of Presentation
      Kumamoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Control of HIV-1 by HIV-1-specific CTL in vitro and in vivo2007

    • Author(s)
      Masafumi, Takiguchi
    • Organizer
      Center for AIDS Research, Kumamoto University, 10th Anniversary Symposium
    • Place of Presentation
      Kumamoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Long-term Control of HIV-1 by HIV-1-Specific CTLs and Appearance of Lower Fitness Virus in HLA-B^*51+ Long term non-progressors2007

    • Author(s)
      Yuka, Kawashima
    • Organizer
      The 37th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Tokyo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] CTL responses to escape mutant of HLA-A^*2402-restrited Nef epitope2007

    • Author(s)
      Mamoru, Fujiwara
    • Organizer
      The 55th Annual Meeting of The Japanese Society for Virology
    • Place of Presentation
      Sapporo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Accumulation of escape mutants by Pol283-8-specific cytotoxic Tlymphocytes in 2 different HLA class I restriction2007

    • Author(s)
      Yuichi Yagita
    • Organizer
      4th International AIDS Society Conference 2007
    • Place of Presentation
      Sydney,Australia
    • Related Report
      2007 Annual Research Report
  • [Presentation] Selection of viral mutations by Pol283-8-specific CTLs in 2 different HLA class I restrictions2007

    • Author(s)
      Yuichi Yagita
    • Organizer
      8th AIDS Seminar in KLTMAMOTO
    • Place of Presentation
      Kumamoto,Japan
    • Related Report
      2007 Annual Research Report
  • [Presentation] Different Ability of Escape Mutant-Specific Cytotoxic T Cells to Suppress Replication of Escape Mutant and Wild-type HIV-1 in New Hosts2007

    • Author(s)
      Mamoru Fujiwara
    • Organizer
      8th AIDS Seminar in KUMAMOTO
    • Place of Presentation
      Kumamoto,Japan
    • Related Report
      2007 Annual Research Report
  • [Presentation] Control of HIV-1 replication by HIV-1-specific CTL in HLA・B*51+long・term non progressors2007

    • Author(s)
      Yuka Kawashima
    • Organizer
      8th AIDS Seminar in KUMAMOTO
    • Place of Presentation
      Kumamoto,Japan
    • Related Report
      2007 Annual Research Report
  • [Presentation] Control of HIV-1 by HIV-1-specifc CTL in vitro and in vivo2007

    • Author(s)
      Masafumi Takiguchi
    • Organizer
      Center for AIDS Research, Kumamoto University, 10th Anniversary Symposium
    • Place of Presentation
      Kumamoto,Japan
    • Related Report
      2007 Annual Research Report
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.caids.kumamoto-u.ac.jp/Virus

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.caids.kumamoto-u.ac.jp/Virus

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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