Identification of host factors responsible for virus paraticle production and screening of its inhibitors through yeast CytoTrap system
| Project/Area Number |
18390144
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Kitasato University |
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Principal Investigator |
MORIKAWA Yuko Kitasato University, 大学院・感染制御科学府, 教授 (20191017)
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| Co-Investigator(Kenkyū-buntansha) |
MOMOSE Fumitaka 北里大学, 大学院・感染制御科学府, 教授 (90332204)
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| Project Period (FY) |
2006 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2008: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2007: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2006: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | 酵母 / ウイルス / 宿主因子 / 阻害剤探索 / CytoTrap / 阻害剤 |
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| Research Abstract |
酵母"CytoTrap法"を用いてHIV粒子形成の責任宿主因子を探索し、2個のGag蛋白に結合する宿主因子(一方は既知の宿主因子AnnexinA2、他方は新規の宿主因子AUP1)を同定した。全長AUP1はHIV粒子産生を促進したが、AUP1 N末端断片は顕著に阻害した。この阻害機序を解析したところ、Gag蛋白分解促進の可能性が推測された。"CytoTrap法"を利用し、酵母でGag-Gag 阻害剤探索系を構築し、化合物ライブラリを探索した。
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] SOCS1 is an inducible host factor during HIV-1 infection and regulates the intracellular trafficking and stability of HIV-1 Gag2008
Author(s)
A. Ryo, N. Tsurutani, K. Ohba, R. Kimura, J. Komano, M. Nishi, H. Soeda, S. Hattori, K. Perrem, M. Yamamoto, J. Chiba, J. Mimaya, K. Yoshimura, S. Matsushita, M. Honda, A. Yoshimura, T. Sawasaki, I. Aoki, Y. Morikawa, & N. Yamamoto
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Journal Title
Proc. Natl. Acad. Sci. USA 105
Pages: 294-299
Related Report
Peer Reviewed
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[Journal Article] Identification of the P-TEFb complex-interacting domain of Brd4 as an inhibitor of HIV-1 replication by functional cDNA library screening in MT-4 cells2008
Author(s)
E. Urano, Y. Kariya, Y. Futahashi, R. Ichikawa, M. Hamatake, H. Fukazawa, Y. Morikawa, T. Yoshida, Y. Koyanagi, N. Yamamoto, & J. Komano
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Journal Title
FEBS Let 582
Pages: 4053-4058
Related Report
Peer Reviewed
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[Journal Article] SOCSl is an inducible host factor during HIV-1 infection and regulates the intracellular trafficking and stability of HIV-1 Gag2008
Author(s)
A. Ryo, N. Tsurutani, K. Ohba, R. Kimura, J. Komano, M. Nishi, H. Soeda, S. Hattori, K. Perrem, M. Yamamoto, J. Chiba, J. Mimaya, K. Yoshimura, S. Matsushita, M. Honda, A. Yoshimura, T. Sawasaki, I. Aoki, Y. Morikawa, & N. Yamamoto
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Journal Title
Proc. Natl. Acad. Sci. USA 105
Pages: 294-299
Related Report
Peer Reviewed
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