| Project/Area Number |
18390218
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Hiroshima University |
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Principal Investigator |
CHAYAMA Kazuaki Hiroshima University, Graduate School of Biomedical Sciences, Professor (00211376)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥14,610,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2007: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2006: ¥8,500,000 (Direct Cost: ¥8,500,000)
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| Keywords | interferon / hepatitis C virus / interferon stimulated genes / expression profile / microarray / mRNA / human hepatocyte chimeric mouse / therapeutic drug / 肝炎ウイルス / ゲノム / キメラマウス / 免疫 |
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| Research Abstract |
We inflicted human hepatocyte chimerie mice with hepatitis B and hepatitis C viruses by injecting serum samples obtained from serum samples of chronic hepatitis B and hepatitis C virus carriers. After confirming the development of viremia in mice, we administered daily 7000 U/g of alpha interferon intramusculary. A rapid decrease in hepatitis C virus RNA levels was observed in all treated mice In contrast, only faint reduction of hepatitis B virus DNA was observed in mice infected with hepatitis B virus. We also administered the same amount of interferon to some human hepatocyte chimeric mice. We sacrificed and collected liver samples from each mice and extracted mRNA from each maim. Using them mRNA samples we performed microarray analysis. We obtained expression profiles from control, non-infected and non-interferon treated mice. We compared expression profiles of mice liver obtained from hepatitis B virus and hepatitis C virus infected mouse as well as interferon treated mice inflicted with hepatitis B and C viruses and treated with interferon. Up regulation of interferon stimulated genes ware observed in hepatitis C virus infected mice The up regulation of them genes was not so eminent in mice infected with hepatitis B virus. Interestingly, up regulation of some interferon stimulated genes were diminished in mice infected with hepatitis C virus compared with non-infected mouse. Further research is now in progress to analyze a mechanism underlining this suppression of interferon stimulated gene production.
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