Research Project
Grant-in-Aid for Scientific Research (B)
High-density single nucleotide polymorphism (SNP) array analysis revealed novel amplification at 1q21 in cell lines derived from hepatocellular carcinomas (HCCs). Fluorescence in situ hybridization and real-time quantitative polymerase chain reaction (PCR) studies verified amplification at 1q21. An increase in copy-number at the region was detected in 32 (89%) of the 36 primary HCC tumors. To identify the targets for amplification, we examined 19 HCC cell lines for expression levels of all 26 genes located within the 700-kb amplified region. Five genes were over-expressed in cell lines with amplification at 1q21. Among these, CREB3L4 (cyclic AMP responsive element binding protein 3-like 4), INTS3 (integrator complex subunit 3) and SNAPAP(snare-associated protein snapin)were significantly over-expressed in tumors from 18 HCC patients when compared with counterpart nontumorous tissues. Our findings suggest that CREB3L4, INTS3 and SNAPAP are probable targets for the amplification mechanism and may therefore be involved, together or separately, in the development and/or progression of HCCs.
All 2008 2007 2006 Other
All Journal Article (15 results) (of which Peer Reviewed: 6 results) Presentation (2 results) Book (2 results)
Cancer Genet Cytogenet 180
Pages: 30-36
Hepatol Res. 38
Pages: 27-36
Pages: 348-353
Pages: 30-6
Hepatol Res 38
Cancer Genet Cytogenet. 180
J Hepatol 45・5
Pages: 681-687
J Hepatol 44・6
Pages: 1074-1082
Oncogene 25・17
Pages: 2537-2545
Hepatology 44・2
Pages: 326-334
Liver Int 26・5
Pages: 613-620
Oncogene (in press)
