| Project/Area Number |
18390242
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Saitama Medical University |
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Principal Investigator |
HAGIWARA Koichi Saitama Medical University, Faculty of Mecicine, Professor (00240705)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2007: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2006: ¥10,400,000 (Direct Cost: ¥10,400,000)
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| Keywords | Homozygosity Fingerprinting / Disease susceptibility gene / GeneChip / Analysis for Identical-by-descent / Autozygous segment / HapMap / Sienificance threshold / COPD / 疾患感受性遺伝子 / ホモ接合ハプロタイプ / SNP / Marfan症候群 / 単因子疾患 / 多因子疾患 / 共通先祖由来断片 |
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| Research Abstract |
The elucidation of the responsible gene for the multigene diseases is a difficult but an important subject. In this area, the whole genome association approach is frequently employed. However, the method is applicable only to diseases of which frequency is high, and the result often lacks reproducibility. We have invented the Homozygosity Fingerprinting method that targets multigene diseases. It is a non-parametric method applicable to recessive genes. We have determined several parameters for the method using Haldane's Poisson process model for the recombination of the chromosome, and established theoretical background for the identification of recessive genes that are derived from a small number of ancestors and now spread into the population. Using the parameter, we analyzed 6 patients with alpha-1 antitrypsin deficiency, with 45 HapMap Japanese data as a control. Although the 6 patients were from different areas of Japan, we could identify the location of alpha1-antitrypsin gene from their genotyping data. With the use of 1000k GeneChip recently available, we are able to identify genes derived from common ancestors who lived 600 years ago. The method is applicable even to the diseases that are caused by several genes, and thus is considered to be useful for the identification of such genes.
The method has already approved Japanese patent (No. 4059517) and now being transferred to international phase.
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