Basic Research for Development of the Polyglutamine Disease Therapeutics by HMGB proteins
| Project/Area Number |
18390254
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
OKAZAWA Hitoshi Tokyo Medical and Dental University, Medical Research Institute, Professor (50261996)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥15,800,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2007: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2006: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | POLYGLUTAMINE / HMGB / therapeutics / spinocerebellar ataxia / Huntington's disease / transcription / DNA repair / 核機能 / 変性 / DNA損傷 / ショウジョウバエ / 細胞死 / 神経細胞 / プロテオーム / 神経変性 |
|---|
| Research Abstract |
Neurodegnerative diseases are caused by aggregation of misfiled disease proteins inside or outside of neurons. The disease proteins are suspected to interact with physiological cellular proteins before aggregation, and to dysfunction or decrease the normal proteins. In polyglutamine diseases including 9 neurodegenerative diseases such as Huntington's disease and familial spinocerebellar ataxias, it is known that nuclear translocation of the disease proteins is essential for the pathology. We examined quantitative change of soluble nuclear proteins through proteome analysis, and found that HMGB proteins are reduced in two polyglutamine diseases. HMGB proteins play critical roles for high architectural change of genomic DNA, thus they are essential for recombination, damage repair, and transcription. We found the reduction of soluble nuclear HMGB proteins leads to an increase of DNA damage signals and a reduction of general transcription. Furthermore, we found that supplementation of HMGB1 protein rescues neurodegeneration in fly models.
From these results, we propose dysfunction of DNA damage repair as a new critical pathology of polyglutamine diseases. Considering that mutations of DNA damage repair genes cause aging disorders, we might be able to hypothesize that polyglutamine diseases are secondary accelerated aging due to the accumulation of conformationally abnormal protins in neurons.
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Report
(3 results)
Research Products
(56 results)
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[Journal Article] Proteome analysis of soluble nuclear proteins reveals that HMGB1/2 suppress genotoxic stress in polyglutamine diseases.2007
Author(s)
Qi ML, Tagawa K, Enokido Y, Yoshimura N, Wada Yi, Watase K, Ishimura SI, Kanazawa I, Botas J, Saitoe M, Wanker E.E, and Okazawa H.
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Journal Title
Nature Cell Biology 9
Pages: 402-414
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Proteome analysis of soluble nuclear proteins reveals that HMGB1/2 suppress genotoxic stress in polyglutamine diseases2007
Author(s)
Qi ML, Tagawa K, Enokido Y, Yoshimura N, Wada Y-I, Watase K, Ishimura SI, Kanazawa I, Botas J, Saitoe M, Wanker E. E,and Okazawa H.
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Journal Title
Nature Cell Biology 9
Pages: 402-414
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Proteome analysis of soluble nuclear proteins reveals that HMGB1/2 suppress genotoxic stress in polyglutamine diseases.2007
Author(s)
Qi M-L, Tagawa K, Enokido Y, Yoshimura N, Wada Y-I, Watase K, Ishimura S-I, Kanazawa I, Botas J, Saitoe M, Wanker E.E, and Okazawa H.
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Journal Title
Nature Cell Biology 9
Pages: 402-414
Related Report
Peer Reviewed
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[Journal Article] Transcriptional repression induces a slowly progressive atypical neuronal death associated with changes of YAP isoforms and p73.2006
Author(s)
Hoshino M, Qi ML, Yoshimura N, Miyashita T, Tagawa K, Wada Y.i, Enokido Y, Marubuchi S, Harjes P, Arai N, Oyanagi K, Blandino G, Sudol M, Rich T, Kanazawa I, Wanker E.E, Saitoe M, and Okazawa H.
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Journal Title
Journal of Cell Biology 172
Pages: 589-604
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Hepatoma-derived growth factor, a new trophic factor for motor neurons, is up-regulated in the spinal cord of PQBP-1 transgenic mice before onset of degeneration.2006
Author(s)
Marubuchi S, Okuda T, Tagawa K, Enokido Y, Horiuchi D, Shimokawa R, Tamura T, Qi ML, Eishi Y, Watabe K, Shibata M, Nakagawa M and Okazawa H
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Journal Title
J Neurochem 99
Pages: 70-83
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
[Journal Article] Transcriptional repression induces a slowly progressive atypical neuronal death associated with changes of YAP isoforms and p732006
Author(s)
Hoshino M, Qi ML, Yoshimura N, Miyashita T, Tagawa K, Wada Yi, Enokido Y, Marubuchi S, Harjes P, Arai N, Oyanagi K, Blandino G, Sudol M, Rich T, Kanazawa 1, Wanker E. E, Saitoe M, and Okazawa H.
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Journal Title
Journal of Cell Biology 172
Pages: 589-604
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
[Journal Article] Hepatoma-derived growth factor, a new trophic factor for motor neurons, is up-regulated in the spinal cord of PQBP-1 transgenic mice before onset of degeneration2006
Author(s)
Marubuchi S, Okuda T, Tagawa K, Enokido Y, Horiuchi D, Shimokawa R, Tamura T, Qi ML, Eishi Y, Watabe K, Shibata M, Nakagawa M and Okazawa H.
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Journal Title
J Neurochem 99
Pages: 70-83
Description
「研究成果報告書概要(欧文)」より
Related Report
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