Analysis of immunoreactivity against glycoproteins with disialosyl residue in neuroimmunological diseases

• Project/Area Number: 18390264
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Kinki University
• Principal Investigator: KUSUNOKI Susumu Kinki University, 医学部, 教授 (90195438)
• Co-Investigator(Kenkyū-buntansha): MIYAMOTO Katsuichi 近畿大学, 医学部, 講師 (50388526)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥2,940,000); Fiscal Year 2008: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2007: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2006: ¥5,200,000 (Direct Cost: ¥5,200,000)
• Keywords: 疫性神経疾患 / 末梢神経障害 / 運動失調 / 糖鎖 / ガングリオシド / 自己免疫 / 脳神経疾患 / 神経科学 / 脳・神経 / 免疫学 / シアル酸 / 末梢神経

Research Abstract

ジシアロシル基をもつGD1b感作によるウサギ失調性ニューロパチ-モデルにおいて、後根神経節細胞のアポトーシスを確認した GD1bにきわめて特異的な抗体が失調の病態に関連することを示した Fisher症候群および関連疾患で、GQ1bを含むガングリオシド複合体に特異的抗体の存在を見出した。ジシアロシル基に反応する抗体と神経組織のタンパク成分の反応をWestern blottingで認めた。

Research Products

• [Journal Article] Apoptosis of primary sensory neurons in GQ1b-induced sensory ataxic neuropathy (2008)
  • Author(s): Takada K, Shimizu J, Kusunoki S
  • Journal Title: Exp Neurol 209 Pages: 279-283
  • Peer Reviewed
• [Journal Article] Roles of complex gangliosides in the development of experimental autoimmune encephalomyelitis (2008)
  • Author(s): Miyamoto K, Takada K, Furukawa K, Furukawa K, Kusunoki S
  • Journal Title: Glycobiology 18 Pages: 408-413
  • Peer Reviewed
• [Journal Article] Ganglioside complexes containing GQ1b as targets in Miller Fisher and Guillain-Barre syndromes (2008)
  • Author(s): Kanzaki M, Kaida K, Ueda M, Morita D, Hirakawa M, Motoyoshi K, Kamakura K, Kusunoki S
  • Journal Title: J Neurol Neurosurg Psychiatry 79 Pages: 1148-1152
  • Peer Reviewed
• [Journal Article] GD1b-specific antibody induces ataxia in Guillain-Barre syndrome (2008)
  • Author(s): Kaida K, Kamakura K, Ogawa G, Ueda M, Motoyohi K, Arita M, Kusunoki S
  • Journal Title: Neurology 71 Pages: 196-201
  • Peer Reviewed
• [Journal Article] Complete recovery of an aged patient with Guillain-Barre syndrome associated with multiple IgM anti-ganglioside antibodies (2008)
  • Author(s): Furiya Y, Hirano M, Kusunoki S, Ueda M, Nishiwaki T, Ueno S
  • Journal Title: Muscle Nerve 38 Pages: 1630-1633
  • Peer Reviewed
• [Journal Article] GM1/GalNAc-GD1a complex: a target for pure motor Guillain-Barre syndrome (2008)
  • Author(s): Kaida K, Sonoo M, Ogawa G, Kamakura K, Ueda M, Arita M, Motoyoshi K, Kusunoki S
  • Journal Title: Neurology 71 Pages: 1683-1690
  • Peer Reviewed
• [Journal Article] Antibodies against gangliosides and ganglioside complexes in Guillain-Barre syndrome : New aspects of research (2008)
  • Author(s): Kusunoki S, Kaida K, Ueda M
  • Journal Title: Bioch Biophys Acta 1780 Pages: 441-444
  • Peer Reviewed
• [Journal Article] Ganglioside complexes as target antigens in Guillain-Barre syndrome and related disorders (2008)
  • Author(s): Kaida K, Kusunoki S
  • Journal Title: Future Lipidol 3 Pages: 425-434
  • Peer Reviewed
• [Journal Article] Ganglioside complexes containing GQ1b as targers in Miller Fisher and Guillain-Barre syndromes. (2008)
  • Author(s): Kanzaki M
  • Journal Title: J Neurol Neurosurg Psychiatry 79 Pages: 1148-1152
  • Peer Reviewed
• [Journal Article] GDlb-specific antibody induces ataxia in Guillain-Barre syndrome. (2008)
  • Author(s): Kaida K
  • Journal Title: Neurology 71 Pages: 196-201
  • Peer Reviewed
• [Journal Article] GM1/GalNAc-GD1a complex : a target for pure motor Guillain-Barre syndrome. (2008)
  • Author(s): Kaida K
  • Journal Title: Neurology 71 Pages: 1683-1690
  • Peer Reviewed
• [Journal Article] Complete recovery of an aged patient with Guillain-Barre syndorme associated with multiple IgM anti-ganglioside antibodies (2008)
  • Author(s): Furiya Y
  • Journal Title: Muscle Nerve 38 Pages: 1630-1633
  • Peer Reviewed
• [Journal Article] Apoptosis of primary sensory neurons in GD 1b-induced sensoryataxic neuropathy (2008)
  • Author(s): Takada K
  • Journal Title: Exp Neurol 209 Pages: 279-283
  • Peer Reviewed
• [Journal Article] Anti-ganglioside complex antibodies associated with severe disability in GBS (2007)
  • Author(s): Kaida K, Morita D, Kanzaki M, Kamakura K, Motoyoshi K, Hirakawa M, Kusunoki S
  • Journal Title: J Neuroimmunol 182 Pages: 212-8
  • Peer Reviewed
• [Journal Article] Guillain-Barre syndrome with antibodies to GD1a/GD1b complex (2007)
  • Author(s): Hamaguchi T, Sakajiri K, Sakai K, Okino S, Sada M, Kusunoki S
  • Journal Title: J Neurol Neurosurg Psychiatry 78 Pages: 548-549
  • Peer Reviewed
• [Journal Article] Gangliosides GM1 and GM3 in the living cell membrane form clusters susceptible to cholesterol depletion and chilling (2007)
  • Author(s): Fujita A, Cheng J, Hirakawa M, Furukawa K, Kusunoki S, Fujimoto T
  • Journal Title: Mol Biol Cell 18 Pages: 2112-2122
  • Peer Reviewed
• [Journal Article] Predictors of respiratory failure in Guillain-Barre syndrome (2007)
  • Author(s): Kusunoki S, van Doom P
  • Journal Title: Nat Clin Pract Neurol 3 Pages: 430-431
  • Peer Reviewed
• [Journal Article] Predictors of resphratory failure in Guillain-Barre syndrome (2007)
  • Author(s): Kusunoki S
  • Journal Title: Nat Clin Pract Neurol 3 Pages: 430-431
  • Peer Reviewed
• [Journal Article] Anti-ganglioside complex antibodies associated with severe disability in GBS. (2007)
  • Author(s): Kaida K, Morita D, Kanzaki M, Kamakura K, Motoyoshi K, Hirakawa M, Kusunoki S.
  • Journal Title: J Neuroimmunol 182 Pages: 212-218
• [Journal Article] Abnormal sudomotor axon reflex and antiganglioside antibodies (2006)
  • Author(s): Kuzumoto Y, Shioyama M, Kihara M, Kusunoki S
  • Journal Title: Muscle Nerve 33 Pages: 828-829
  • Peer Reviewed
• [Journal Article] Anti-ganglioside complex antibodies in Miller Fisher syndrome (2006)
  • Author(s): Kaida K, Kanzaki M, Morita D, Kamakura K, Motoyoshi K, Hirakawa M, Kusunoki S
  • Journal Title: J Neurol Neurosurg Psychiatry 77 Pages: 1043-1046
  • Peer Reviewed
• [Journal Article] Anti-ganglioside complex antibodies in Miller Fisher syndrome. (2006)
  • Author(s): Kaida K, Kanzaki M, Morita D, Kamakura K, Motoyoshi K, Hirakawa M, Kusunoki S.
  • Journal Title: J Neurol Neurosurg Psychiatry 77 Pages: 1043-1046
• [Journal Article] Selective COX-2 inhibitor celecoxib prevents experimental autoimmune encephalomyelitis through COX-2 independent pathway. (2006)
  • Author(s): Miyamoto K, Miyake S, Mizuno M, Oka N, Kusunoki S, Yamamura T.
  • Journal Title: Brain 129 Pages: 1984-1992
• [Journal Article] Abnormal sudomotor axon reflex and antiganglioside antibodies. (2006)
  • Author(s): Kuzumoto Y, Shioyama M, Kihara M, Kusunoki S.
  • Journal Title: Muscle Nerve 33 Pages: 828-829
• [Journal Article] Effect of the aldose reductase inhibitor fidarestat on experimental diabetic neuropathy in the rat. (2006)
  • Author(s): Kuzumoto Y, Kusunoki S, Kato N, Kihara M, Low PA.
  • Journal Title: Diabetologia 49 Pages: 3085-3093
• [Journal Article] Antibodies against gangliosides and ganglioside complexes in Guillain-Barre syndrome:New aspects of research
  • Author(s): Kusunoki S
  • Journal Title: Bioch Biophys Acta (in press)
  • Peer Reviewed
• [Journal Article] Guillain-Barre syndrome with antibodies to GD1a/GD1b complex.
  • Author(s): Hamaguchi T, Sakajiri K, Sakai K, Okino S, Sada M, Kusunoki S.
  • Journal Title: J Neurol Neurosurg Psychiatry, (in press)
• [Presentation] Antibodies to gangliosides and ganglioside complexes in autoimmune neuropathies (2008)
  • Author(s): Kusunoki S
  • Organizer: Ninth International Congress of Neuroimmunology
  • Place of Presentation: Fort Worth, Texas, USA
• [Presentation] Serological characteristics of Bickerstaffs brainstem encephalitis : Comparison with Miller Fisher and Guillain-Barre syndromes (2008)
  • Author(s): Kusunoki S
  • Organizer: Inflammatory Neuropathy Consortium (INC) Meeting
  • Place of Presentation: Paris, France
• [Presentation] シンポジウム「末梢神経障害の研究-最近の進歩-」 : 免疫関連性ニューロパチー (2008)
  • Author(s): 楠進
  • Organizer: 第49回日本神経学会総会
  • Place of Presentation: 横浜
• [Presentation] Antibodies to gangliosides and ganglioside complexes in autoimmune neuropathies. (2008)
  • Author(s): Kusunoki S
  • Organizer: Ninth International Congress of Neuroimmunology
  • Place of Presentation: Fort Worth, TX, USA
• [Presentation] 「末梢神経障害の研究-最近の進歩-」 : 免疫関連性ニューロパチー (2008)
  • Author(s): 楠進
  • Organizer: 第49回日本神経学会総会
  • Place of Presentation: 横浜
• [Presentation] 炎症と末梢神経疾患 (2007)
  • Author(s): 楠進
  • Organizer: 日本内科学会学術集会 : 第35回内科学の展望「慢性炎症と内科疾患」
  • Place of Presentation: 神戸
  • Year and Date: 2007-11-18
• [Presentation] IgG antibodies highly specific to GD1b in Guillain-Barre syndrome with ataxia (2007)
  • Author(s): Kusunoki S
  • Organizer: 132nd Annual Meeting of American Neurological Association
  • Place of Presentation: Washington DC, USA
• [Presentation] Anti-ganglioside complex antibodies in Miller Fisher syndrome and Guillain-Barre syndrome with ophthalmoplegia (2007)
  • Author(s): Kaida K
  • Organizer: 132nd Annual Meeting of American Neurological Association
  • Place of Presentation: Washington DC, USA
• [Presentation] IgG antibody highly specific to GD1b is specifically associated with ataxia in Guillain-Barre syndrome (2007)
  • Author(s): Kusunoki S
  • Organizer: 2007 Meeting of the Peripheral Nerve Society
  • Place of Presentation: Snowbird, Utah, USA
• [Presentation] Anti-ganglioside complex antibodies in Miller Fisher syndrome and Guillain-Barre syndrome with ophthalmoplegia (2007)
  • Author(s): Kaida K
  • Organizer: 2007 Meeting of the Peripheral Nerve Society
  • Place of Presentation: Snowbird, Utah, USA
• [Presentation] Antibodies against ganglioside complexes in Guillain-Barre syndrome ; New aspects of research (2007)
  • Author(s): Kusunoki S
  • Organizer: Glycobiology and Sphingobiology 2007 - Hakomori Commemorative Forum -
  • Place of Presentation: Tokushima, Japan
• [Presentation] IgG antibody highly specific to GD 1b is specifically associated withataxia in Gmllain-Barre syndrome (2007)
  • Author(s): Kusunoki S
  • Organizer: 2007 Meeting of the Peripheral Nerve Society
  • Place of Presentation: Snowbird,Utah,USA
• [Presentation] GD1b感作によるウサギ失調性ニューロパチーは後根神経節細胞のアポトーシスによる (2007)
  • Author(s): 高田 和男
  • Organizer: 第48回日本神経学会総会
  • Place of Presentation: 名古屋
• [Presentation] Going solo or in need of a friend : Ganglioside and lipid clusters form novel antigenic determinations in GBS (2006)
  • Author(s): Kusunoki S
  • Organizer: Eighth International Conference of Neuroimmunology
  • Place of Presentation: Nagoya, Japan, J Neuroimmunol 178(Suppl 1): 30, 2006
• [Presentation] Anti-GD1a/GD1b complex antibody is associated with severe Guillain-Barre syndrome (2006)
  • Author(s): Kusunoki S
  • Organizer: 131st Annual Meeting of the American Neurological Association
  • Place of Presentation: Chicago, USA, Ann Neurol 60 (suppl): S20, 2006
• [Book] 糖鎖に反応する抗体は神経を破壊する (2009)
  • Author(s): 楠進
  • Publisher: 株式会社クバプロ (東京)
• [Book] Antiglycolipid antibodies in autoimmune neuropathies ; new aspects of research. In, Neuroimmunology Research Focus (ed. by Broglio PV), pp85-99 (2007)
  • Author(s): Kusunoki S
  • Publisher: Nova Science Publishers, Inc., New York, USA
• [Book] Neuroirnmunology Research Focus (ed by Broglio PV, Nova Science Publishers, Inc., New York, USA) (2007)
  • Author(s): Kusunoki S
  • Publisher: Antiglycolipid antibodies in autoimmune neuropathies;new aspects of research
• [Book] Anti-ganglioside complex antibodies in Miller Fisher syndrome. In, Current Topics in Neuroimmunology (ed. by Tabira T, Yamamura T, Kira J. Medimond) pp223-228 (2006)
  • Author(s): Kaida K, Kamakura K, Kusunoki S