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Analyses of a cation transporters during immune and inflammatory responses

Research Project

Project/Area Number 18390287
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionThe University of Tokyo

Principal Investigator

YAMAMOTO Kazuhiko  The University of Tokyo, Hospital, Professor (80191394)

Co-Investigator(Kenkyū-buntansha) SAWADA Tetsuji  The University of Tokyo, Hospital, Assistant Professor (50235470)
KAWAHATA Kimito  The University of Tokyo, Hospital, Assistant Professor (70334406)
FUJIO Keishi  The University of Tokyo, Hospital, Assistant Professor (70401114)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,830,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2007: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2006: ¥7,600,000 (Direct Cost: ¥7,600,000)
KeywordsImmunology / genetics / internal medicine / 遺伝学 / 関節リウマチ / 疾患関連遺伝子 / 有機カチオントランスポーター / SLC22A4 / サイトカイン / エルゴチオネイン
Research Abstract

Recent genetic studies suggested that an cation transporter, SLC22A4, is associated with immune and inflammatory disorders. We reported a single nucleotide polymorphism, SNP, is associated with Japanese rheumatoid arthritis. Peltekova V. et al also reported that a non-synonymous SNP on SLC22A4 and a SNP on SLC22A5 that is strong linkage disequilibrium to the former SNP is associated with Crohn's disease. Since rheumatoid arthritis and Crohn's disease are immune and inflammatory disorders, it is highly possible that such cation transporters are involved in the reactions.
We thus tried to analyze the function of SLC22A4 in the immune and inflammatory conditions. We also tried to find out the ways to control the reactions by modulating the function of SLC22A4.
We made first siRNAs to knock down endogenous SLC22A4. One of the possible substrates of the transporter is ergothioneine. However, we do not know the half life and metabolism of ergothioneine. Thus we set up transient as well as constitutive knock down systems. We also introduce the SLC22A4 gene into cells by plasmid vector methods and retrovial vectors. We are now examining the possible role of SLC22A4 on cytokine expressions using these SLC22A4 modified cells.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (21 results)

All 2007 2006

All Journal Article (21 results) (of which Peer Reviewed: 6 results)

  • [Journal Article] Hepatocyte growth factor significantly suppresses collagen-induced arthritis in mice.2007

    • Author(s)
      Okunishi K, Dohi M, Fujio K, Nakagome K, Yamamoto K., 他7名
    • Journal Title

      J. Immunol. 179

      Pages: 5504-5513

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Lessons from a Genomewide Association Study of Rheumatoid Arthritis.2007

    • Author(s)
      Yamamoto K, Yamada R.
    • Journal Title

      N. Engl. J. Med. 357

      Pages: 1250-1251

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Interleukin-17B and interleukin-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis.2007

    • Author(s)
      Yamaguchi Y, Fujio K, Shoda H, Okamoto A, Yamamoto K., 他2名
    • Journal Title

      J. Immunol. 179

      Pages: 7128-7136

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Hepatocyte growth factor significantly suppresses collagen-induced arthritis in mice2007

    • Author(s)
      Okunishi K, Dohi M, Fuiio K, Nakagome K, Tabata Y, Okasora T, Seki M, Shibuya M, Imamura M, Harada H, Tanaka R, Yamamoto K.
    • Journal Title

      J Immunol. 179

      Pages: 5504-5513

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Lessons from a Genomewide Association Study of Rheumatoid Arthritis2007

    • Author(s)
      Yamamoto K, Yamada R.
    • Journal Title

      N. Engl. J. Med. 357

      Pages: 1250-1251

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Interleukin-17B and interleukin-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis2007

    • Author(s)
      Yamaguchi Y, Fujio K, Shoda H, Okamoto A, Tsuno NH, Takahashi K, Yamamoto K.
    • Journal Title

      J Immunol. 179

      Pages: 128-136

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Antigen-specific immunotherapy for autoimmune diseases2007

    • Author(s)
      Yamamoto K, Okamoto A, Fuiio K.
    • Journal Title

      Expert Opin Biol Ther. 7

      Pages: 359-367

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Hepatocyte growth factor significantly suppresses collagen-induced arthritis in mice2007

    • Author(s)
      Okunjshi K, Dohi M, Fujio K, Nakagome K, Yamamoto K., 他7名
    • Journal Title

      J Immunol 179

      Pages: 5504-5513

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lessons from a Genomewide Association Study of Rheumatoid Arthritis2007

    • Author(s)
      Yamamoto K, Yamada R.
    • Journal Title

      N. Engl. J. Med 357

      Pages: 1250-1251

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interleukin-17B and interleukin-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis2007

    • Author(s)
      Yamaguchi Y, Fujio K, Shoda H, Okamoto A, Yamamoto K., 他2名
    • Journal Title

      J Immunol 179

      Pages: 7128-7136

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antigen-specific immunotherapy for autoimmune diseases.2007

    • Author(s)
      Yamamoto K.
    • Journal Title

      Expert Opin Biol Ther. 7

      Pages: 359-367

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Gene therapy of arthritis with TCR isolated from the inflamed paw2006

    • Author(s)
      Fuiio K, Okamoto A, Araki Y, Shoda H, Tahara, Tsuno NH, Takahashi K, Kitamura T, Yamamoto K.
    • Journal Title

      J Immunol. 177

      Pages: 8140-8147

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Splenic dendritic cells induced by oral antigen administration are important for the transfer of oral tolerance in an experimental model of asthma2006

    • Author(s)
      Nagatani K, Dohi M, To Y, Tanaka R, Okunichi K, Nakagome K, Sagawa K. Tanno Y, Komagata Y, Yamamoto K.
    • Journal Title

      J Immunol. 176

      Pages: 1481-1489

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Idetification of citrullinated eukaryotic translation initiation factor 4G1 as novel autoantigen in rheumatoid in rheumatoid arthritis2006

    • Author(s)
      Okazaki Y, Suzuki A, Sawada T, Ohtake-Yamanaka M, Inoue T, Hasebe T, Yamada R, Yamamoto K.
    • Journal Title

      Biochem Biophys Res Commun 341

      Pages: 94-100

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Antigen sensitized CD4^+CD62L^<low> memory/effector T helper 2 cells can induce airway hyperresponsiveness in an antigen free setting2006

    • Author(s)
      Nakagome K, Dohi M, Okunishi K, To Y, Sato A, Komagata Y, Nagatani K, Tanaka R, Yamamoto K.
    • Journal Title

      Respir Res. 28

      Pages: 6-46

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The genetics of systemic lupus erythematosus : differences across ethnicities2006

    • Author(s)
      Kochi Y, Shimane K, Yamamoto K.
    • Journal Title

      APLAR J Rheumatology 9

      Pages: 353-358

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Gene therapy of arthritis with TCR isolated from the inflamed paw.2006

    • Author(s)
      Fujio K.
    • Journal Title

      J Immunol. 177

      Pages: 8140-8147

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Splenic dendritic cells induced by oral antigen administration are important for the transfer of oral tolerance in an experimental model of asthma.2006

    • Author(s)
      Nagatani K.
    • Journal Title

      J Immunol. 176

      Pages: 1481-1489

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Idetification of citrullinated eukaryotic translation initiation factor 4G1 as novel autoantigen in rheumatoid in rheumatoid arthritis.2006

    • Author(s)
      Okazaki Y.
    • Journal Title

      Biochem Biophys Res Commun. 341

      Pages: 94-100

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Antigen-sensitized CD4^+CD62L^<low> memory/effector T helper 2 cells can induce airway hyperresponsiveness in an antigen free setting.2006

    • Author(s)
      Nakagome K.
    • Journal Title

      Respir Res. 28

      Pages: 6-46

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The genetics of systemic lupus erythematosus : differences across ethnicities.2006

    • Author(s)
      Kochi Y.
    • Journal Title

      APLAR J Rheumatology. 9

      Pages: 353-358

    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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