| Budget Amount *help |
¥17,750,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2007: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2006: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Research Abstract |
There is no fundamental therapeutic method far muscular dystrophy, therefore the fates of these patients axe serious. To improve the quality of life for these patients, the development of various therapeutic strategiesis is under investigation. Embryonic stem cell is one promising candidate. In this research, we developed the method to produce and isolate skeletal muscle stem cells, satellite cells, from mouse ES cells Following the formation of embryoid bodyies, the culture was continued on math gel. During this process, the formation of differentiated skeletal muscles was confirmed. Along with this, skeletal muscle stem cells, satellite cells, were identified by specific antibody, SM/C-2.6. Identified cells differentiate into muscle, cell in vitro. Simultaneously, transplantation of these cell in MDX mice (muscular dystrophy mice) resulted into the formation of muscle fibers. Furthermore, mechanical muscle damage led to the amplification of SM/C-2.6 positive cells, resulting in the increased number of skeletal muscle stem cells. In addition, success of second transplantation of these cells revealed the definite character of muscle stem cells.
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