Establishment of therapeutic strategy for degenerative muscular disease by embryonic stem mils
| Project/Area Number |
18390298
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto University |
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Principal Investigator |
HEIKE Toshio Kyoto University, Graduate School of Medicine, Associate Professor (90190173)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAMATSU Hidefumi Kyoto University, Graduate School of Medicine, Assistant Professor (40362503)
UMEDA Katsutsugu Kyoto University, Graduate School of Medicine, Assistant Professor (80397538)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥17,750,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2007: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2006: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | ES cell / skeletal muscle stem cell / satellite cell / muscular dystrophy / regeneration / 骨格筋 / 遺伝子治療 / embryoid body |
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| Research Abstract |
There is no fundamental therapeutic method far muscular dystrophy, therefore the fates of these patients axe serious. To improve the quality of life for these patients, the development of various therapeutic strategiesis is under investigation. Embryonic stem cell is one promising candidate. In this research, we developed the method to produce and isolate skeletal muscle stem cells, satellite cells, from mouse ES cells Following the formation of embryoid bodyies, the culture was continued on math gel. During this process, the formation of differentiated skeletal muscles was confirmed. Along with this, skeletal muscle stem cells, satellite cells, were identified by specific antibody, SM/C-2.6. Identified cells differentiate into muscle, cell in vitro. Simultaneously, transplantation of these cell in MDX mice (muscular dystrophy mice) resulted into the formation of muscle fibers. Furthermore, mechanical muscle damage led to the amplification of SM/C-2.6 positive cells, resulting in the increased number of skeletal muscle stem cells. In addition, success of second transplantation of these cells revealed the definite character of muscle stem cells.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] a4 integrin+ endothelium derived from primate embryonic stem cells generates both primitive and definitive hematopoietic cells2007
Author(s)
Shinoda, G., Umeda, K., Heike, T., Arai, M., Niwa, A., Ma, F., Suemori, H., Luo, HY., HK., Chui, D., Torii, R., Shibuya, M., Nakatsuji, N., Nakahata, T
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Journal Title
Blood 109
Pages: 2406-2415
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Isolation and characterization of bone marrow-derived mesenchymal progenitor cells with myogenic and neuronal properties2007
Author(s)
Shiota, M., Heike, T., Haruyama, M., Baba, S., Tsuchiya, A., Fujino, H., Kobayashi, H., Kato, T., Umeda, K., Yoshimoto, M., Nakahata, T
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Journal Title
Exp Cell Res 313
Pages: 1008-1023
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Generation of Cardiac and Endothelial Cells from Neonatal Mouse Testis-derived Multipotent Germline2007
Author(s)
Baba, S., Heike, T., Umeda, K., Iwasa, T., Kaichi, S., Hiraumi, Y., Doi, H., Yoshimoto, M., Kanatsu-Shinohara, M., Shinohara, T., Nakahata, T
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Journal Title
Stem Cells 25
Pages: 1375-1383
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Human cord blood CD34+ cells develop into hepatocytes in the livers of NOD/SCID/ycnull mice through cell fusion2007
Author(s)
Fujino, H., Hiramatsu, H., Tsuchiya, A., Niwa, A., Noma, H., Shiota, M., Umeda, K., Yoshimoto, M., Ito, M., Heike, T., Nakahata, T
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Journal Title
FASEB J 21
Pages: 3499-3510
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification and characterization of hemangiogenic progenitors during cynomolous monkey embryonic stem cell differentiation2006
Author(s)
Umeda, K., Heike, T., Yoshimoto, M., Shinoda, G., Shiota, M., Suemori, H., Luo, HY., Chui, DHK., Torii, It., Shibuya, M., Nakatsuji, N., Nakahata, T
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Journal Title
Stem Cells 24
Pages: 1348-1358
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Sequential analysis of a- and b-globin gene expression during erythropoietic differentiation from primate embryonic stem cells2006
Author(s)
Umeda, K., Heike, T., Nakata-Hizume, M., Niwa, A., Arai, M., Shinoda, G., Ma, F., Suemori, H., Luo, HY., Chui, DH., Torn, It., Shibuya, M., Nakatsuj,i N., Nakahata, T
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Journal Title
Stem Cells 24
Pages: 2627-2636
Description
「研究成果報告書概要(欧文)」より
Related Report
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