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Analyses on the pathogenesis of hydrocephalus associated with L1CAM-mutation using in utero shRNA silencing

Research Project

Project/Area Number 18390308
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Embryonic/Neonatal medicine
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

ITOH Kyoko  Kyoto Prefectural University of Medicine, Department of Pathology and Applied Neurobiology, Associate Professor (80243301)

Co-Investigator(Kenkyū-buntansha) FUSHIKI Shinji  Kyoto Prefectural University of Medicine, Department of Pathology and Applied Neurobiology, Professor (80150572)
IKEDA Hisafumi  Osaka University, Department of Pharmaoology, lecturer (70322493)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥18,770,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2007: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2006: ¥3,300,000 (Direct Cost: ¥3,300,000)
Keywordscongenital malformation / L1CAM / Hydrocephalus / shRNA / in utero electroporation / L1cam / 大脳皮質形成 / PNA / レーザーマイクロダイセクション
Research Abstract

L1cam-silencing using in utero electroporation of shRNA for L1cam (L1-shRNA) was performed in order to gain insight into the pathogenesis of hydrocephalus associated with L1cam mutation. Transfection of L1-shRNA induced down-regulation of the expression of both L1cam mRNA and protein in cultured neuronal cells (Neuro2a) as compared to those transfected by negative control shRNA (shNC). When transfected to the cortical neurons that were cultured from mouse forebrain at embryonal 13.5 days (E13.5), L1-shRNA induced morphological changes, such as increased frequency of neurons with short and multiple neurites and abundant spines. L1-shRNA resulted in decreased length of total neurites and increased numbers of arborization as compared to those transfected by shNC. In utero electroporation of L1-shRNA was performed into the ventricular zone of murine forebrain at E13.5 days. On 2 to 4 days after transfection, the pattern of neuronal differentiation and migration showed no significant differences between L1-shRNA- and shNC-treated brains.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (1 results)

All 2008

All Journal Article (1 results) (of which Peer Reviewed: 1 results)

  • [Journal Article] Ionizing radiation downregulates ASPM, a gene responsible for microcephaly in humans2008

    • Author(s)
      Fujimori A, Yaoi T, Ogi H, Wang B, Suetomi K, Sekine E, Yu D, Kato T, Takahashi S, Okayasu R, Itoh K, Fushiki S.
    • Journal Title

      Biochem Biophys Res Commun 369(3)

      Pages: 953-957

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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