A basic study on the development of bioartificial pancreas using a highly differentiated human pancreatic beta cell line.
| Project/Area Number |
18390347
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
KOBAYASHI Naoya Okayama University, Hospital of Medicine and Dentistry, Senior Assistant Professor (10325102)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMATSUJI Tomoki Okayama University, Hospital of Medicine and Dentistry, Assistant Professor (40379730)
TABATA Yasuhiko Kyoto University, Institute for Frontier Medical Sciences, Professor (50211371)
KUWAGI Kenya Okayama University of Science, Faculty of Engineeting, Associate Professor (80302917)
KATO Jun Okayama University, Hospital of Medicine and Dentdstry, Assistant Professor (90379743)
KONDO Eisaku Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sriences, Associate Professor (30252951)
岩垣 博巳 岡山大学, 医学部・歯学部附属病院, 講師 (50240867)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥17,970,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2007: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2006: ¥6,400,000 (Direct Cost: ¥6,400,000)
|
|---|
| Keywords | Diabetes / Cell Tissue / Tissue Eneineerin / Regenerative Medicine / Biotechnology |
|---|
| Research Abstract |
We originally established a highly differentiated human pancreatic beta cell line. Therefore, we performed a basic study on the development of an implantable typed bioartificial pancreas (BAP) using the cell line for the treatment of diabetes.
1. The human pancreatic beta cell line was functionally cultured with a self-assembling peptide, PuraMatrix, to form islet-like struchture.
2. We constructed a BAP device in which the inner side is composed of high-density polyethylene (HDPE) coated with cellular adhesive polypeptides and the outer side is the membrane of ethylene venyl alcohol (EVAL). Freshly isolated rat islets attached on the surface of HDPE very well.
3. The BAP device was implanted in the intraabdominal cavity with be lapped by the greater omentum. When the outer side of the BAP device was coated by gelatinized bFGF, the number of the induced vessels around the device increased at 1.2-fold.
4. When 2,000 of freshly isolated rat islets injected into the BAP device, function of the islets were maintained for 28 days in vitro.
|
Report
(3 results)
Research Products
(53 results)
