| Budget Amount *help |
¥16,810,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2006: ¥12,000,000 (Direct Cost: ¥12,000,000)
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| Research Abstract |
The interactions between cancer and its micro-environment play a crucial role in tumor growth, invasion and metastasis. Both tenascin-C (TNC) and tenascin inducing factor (TIF) are very important, as the regulatory molecules, for the control of the interactions.
The purpose of this experiment is to develop the immunotherapy which inhibits the tumor growth, invasion and metastasis. Therefore, we examined the role of the stromal factors (SFs) containing TIF in the cell growth and the TNC gene expression, using skin cancer (SC), melanoma (M), breast cancer (BC) and ovarian cancer (OC).
Furthermore, we examined to know whether the anti-TNC antibody and anti-TIF antibody inhibit the tumor growth of those cells or not.
As results
1. Although SFs up-regulated INC gene expression in cancerous cells that don't produce TNC alone, SFs down-regulated the gene expression in cancerous cells that produce 'TNC autonomously.
2. SFs induced not only TNC gene expression in BC, but also EGF receptor gene expression.
3. As to cell growth of those cells, SFs helped the cell growth of SC and BC, but not M.
4. Both antibodies inhibited the tumor growth (SC, M, BC & OC).
In conclusion, these data indicate that TNC and TIF play a pivotal role in the interactions between the cancerous cells and the surrounding stromal cells, and that anti-cancer treatment using anti-TN antibody and anti-TIF antibody, may be effective against the inhibition of cancer growth.
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