Ligand-toxin producing cell containing micro-capsule cancer therapy

• Project/Area Number: 18390359
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: University of Tsukuba
• Principal Investigator: ODA Tatsuya University of Tsukuba, 大学院・人間総合科学研究科, 講師 (20282353)
• Co-Investigator(Kenkyū-buntansha): SHOUDA Junichi 筑波大学, 大学院・人間総合科学研究科, 准教授 (90241827) ; NOGUCHI Masayuki 筑波大学, 大学院・人間総合科学研究科, 教授 (00198582) ; KONDO Tadashi 筑波大学, 大学院・人間総合科学研究科, 講師 (00375495) ; 川上 浩司 東京大学, 医学部附属病院, 客員准教授 (70422318) ; 松本 邦夫 大阪大学, 医学系研究科, 准教授 (90201780) ; 杉浦 慎治 独立行政法人産業技術総合研究所, バイオニクス研究センター, 研究員 (10399496)
• Co-Investigator(Renkei-kenkyūsha): KAWAKAMI Kouji 東京大学, 医学部附属病院, 客員助教授 (70422318) ; MATSUMOTO Kunio 大阪大学, 医学系研究科, 助教授 (90201780) ; SUGIURA Shinji 独立行政法人産業技術総合研究所, バイオニクス研究センター, 研究員 (10399496)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥11,190,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥1,590,000); Fiscal Year 2008: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2006: ¥4,300,000 (Direct Cost: ¥4,300,000)
• Keywords: 膵臓外科学 / マイクロカプセル / 癌治療 / c-Met / ドラッグデリバリー

Research Abstract

腫瘍細胞表面に高発現しているc-Metを標的として、そのリガンドであるHGFのc-Met結合部位(=NK1)に緑膿菌外毒素(Pseudomonas Exotoxin=PE) を結合させた新規の抗癌融合蛋白(=NK1-PE)の開発を行った。NK1-PEによる殺細胞効果はc-Metの発現が低い細胞株(MiaPaca-2、BxPc-3)である程度認められたが、IL4-PEなどと比べると1/100~1/1000の効果しかなかった。PEにより殺細胞効果を上げるというハイリスクな挑戦は結果としては今の所達成出来ていない。

Research Products

• [Journal Article] The measurement of small magnetic signals from magnetic nanoparticles attached to the cell surface and surrounding living cells using a general-purpose SQUID magnetometer (2009)
  • Author(s): Hashimoto S, Oda T, Yamada K, Takagi M, Enomoto T, Ohkohchi N
  • Journal Title: Physics in Medicine and Biology 54(8) Pages: 2571-2583
  • Peer Reviewed
• [Journal Article] Tubula gel fabrication and cell encapsulation in laminar flow stream formed by microfabricated nozzle array (2008)
  • Author(s): Sugiura S, Oda T, Aoyagi Y, Satake SugiurM, Ohkohchi N, Nakajima M.
  • Journal Title: Lab Chip. 8(8) Pages: 1255-7
  • Peer Reviewed
• [Journal Article] 磁性ナノ粒子の癌細胞特異的相乗的集積法の開発 (2008)
  • Author(s): 山田圭一、小田竜也、橋本真治、大河内信弘、柳原英人、喜多英治、高木俊之、金森敏幸
  • Journal Title: 化学工業 59(11) Pages: 69-73
  • Peer Reviewed
• [Journal Article] Tubular gel fabrication and cell encapsulation in laminar flow stream formed by microfabricated nozzle array. (2008)
  • Author(s): Sugiura S, Oda T, Aoyagi Y, Satake M, Ohkohchi N, Nakajima M.
  • Journal Title: LabChip 8(8) Pages: 1255-1257
  • Peer Reviewed
• [Journal Article] 磁性ナノ粒子の癌細胞特異的相乗的集積法の開発 (2008)
  • Author(s): 山田圭一、 小田竜也、 橋本真治、 大河内信弘、 柳原英人、 喜多英治、 高木俊之、 金森敏幸
  • Journal Title: 化学工業 59(11) Pages: 69-73
  • Peer Reviewed
• [Journal Article] Establishment and molecular profiling of a novel human pancreatic cancer panel for 5-FU. (2008)
  • Author(s): Yanagihara K, Takigahira M, Tanaka H, Arao T, Aoyagi Y, Oda T
  • Journal Title: Cancer Science 99(9)
  • NAID: 10024011346
  • Peer Reviewed
• [Journal Article] Microfabricated airflownozzle for microencapsulation of living cells into 150 micrometer microcapsules (2007)
  • Author(s): Sugiura S, Oda T, Aoyagi Y, Matsuo R, Enomoto T, Matsumoto K, Nakamura T, Satake M, Ochiai A, Ohkohchi N, Nakajima M.
  • Journal Title: Biomed Microdevices 9(1) Pages: 91-99
  • Peer Reviewed
• [Journal Article] Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules. (2007)
  • Author(s): Sugiura S, Oda T, Aoyagi Y, Matsuo R, Enomoto T, Matsumoto K, Nakamura T, Satake M, Ochiai A, Ohkohchi N, Nakajima M.
  • Journal Title: Biomed Microdevices. 9(1) Pages: 91-9
• [Journal Article] Consistent liver metastases in a rat model by portal injection of microencapsulated cancer cells (2006)
  • Author(s): Enomoto T, Oda T, Aoyagi Y, Sugiura S, Nakajima M, Satake M, Noguchi M, Ohkohchi N
  • Journal Title: Cancer Research 66(23) Pages: 11131-11139
  • Peer Reviewed
• [Journal Article] Detection of hepatic metastases using ferucarbotran-enhanced MR imaging : feasibility and diagnostic accuracy of three-dimensional sensitivity-encoding water-excitation multishot echo-planar sequence (3D-SWEEP) (2006)
  • Author(s): Mori K, Takahashi N, Hiratsuka M, Shiigai M, Minami M, Oda T, ohkohchi N, Morishita Y
  • Journal Title: J Magn Reson Imaging 24(5) Pages: 1110-1116
  • Peer Reviewed
• [Journal Article] Consistent liver metastases in a rat model by portal injection of microencapsulated cancer cells. (2006)
  • Author(s): Enomoto T, Oda T, Aoyagi Y, Sugiura S, Nakajima M, Satake M, Noguchi M, Ohkohchi N.
  • Journal Title: Cancer Res. 66(23) Pages: 11131-9