• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of new diagnostic markers and drug based on comprehensive search of gene and protein in hepatocellular carcinoma

Research Project

Project/Area Number 18390366
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionYamaguchi University

Principal Investigator

OKA Masaaki  Yamaguchi University, 大学院・医学系研究科, 教授 (70144946)

Co-Investigator(Kenkyū-buntansha) NAKAMURA KAZUYUKI  山口大学, 医学系研究科, 教授 (90107748)
IIZUKA NORIO  山口大学, 医学系研究科, 准教授 (80332807)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2007: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2006: ¥6,500,000 (Direct Cost: ¥6,500,000)
Keywords肝癌 / ゲノム / マイクロアレイ / プロテオーム / バイオテクノロジー / 癌 / 遺伝子 / 蛋白 / 診断 / 創薬
Research Abstract

肝癌の新規診断と創薬の研究を行った。新規診断では、カスタムチップおよび定量的PCR による肝切除後早期再発予測システムを開発した。タンパク診断では、肝癌の新たなマーカーとして3 種類(HSP70、PRX、Mn-SOD)の自己抗体を検出できた。創薬としては、ID2 遺伝子およびENO1 タンパクが標的になること、さらにはID2 発現がHDAC 阻害剤の分子マーカーとなることを発見した。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (65 results)

All 2009 2008 2007 2006 2005

All Journal Article (28 results) (of which Peer Reviewed: 18 results) Presentation (34 results) Book (3 results)

  • [Journal Article] Concomitant overexpression of heat-shock protein 70 and HLA class-I in hepatitis C virus-related hepatocellular carcinoma.2009

    • Author(s)
      Yoshida S.
    • Journal Title

      Anticancer Research 29

      Pages: 539-544

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glycolysis module activated by hypoxia-inducible factor 1alpha is related to the aggressive phenotype of hepatocellular carcinoma2008

    • Author(s)
      Hamaguchi T, Iizuka N, Tsunedomi R, Hamamoto Y, Miyamoto T, Iida M, Tokuhisa Y, Sakamoto K, Takashima M, Tamesa T, Oka M.
    • Journal Title

      Int J Oncol 33

      Pages: 725-731

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Translational microarray systems for outcome prediction of hepatocellular carcinoma2008

    • Author(s)
      Iizuka N, Oka M, Tsunedomi R, Hamamoto Y.
    • Journal Title

      Cancer Sci 99

      Pages: 659-665

    • NAID

      10024004482

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A three-gene predictor for earlyintrahepatic recurrence ofhepatocellular carcinoma after curativehepatectomy2008

    • Author(s)
      Somura H, Iizuka N, Tamesa T, Sakamoto K, Hamaguchi T, Tsunedomi R, Yamada-Okabe H, Sawanura M, Eramoto M, Miyamoto T, Hamamoto Y, Oka M.
    • Journal Title

      Oncology Rep 19

      Pages: 489-495

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] ID2 Expression is Associated with a Risk of Hepatocellular Carcinoma Progression2008

    • Author(s)
      Tsunedomi R, Iizuka N, Yamada-Okabe H, Tamasa T, Okada T, Sakamoto K, Takashima M, Hamaguchi T. Miyamato T, Uchimura S, Hamamoto Y, Oka M.
    • Journal Title

      Clin Cancer Res 14

      Pages: 1025-1031

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Validating a Markov Model of Treatment for Hepatitis C Virus-related Hepatocellular Carcinoma.2008

    • Author(s)
      Ishida H.
    • Journal Title

      METHODS OF INFORMATION IN MEDICINE 47

      Pages: 529-540

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glycolysis module activated by hypoxia-inducible factor lalpha is related to the aggressive phenotype of hepatocellular carcinoma.2008

    • Author(s)
      Hamaguchi T.
    • Journal Title

      International Journal of Oncology 33

      Pages: 725-731

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relation between serum levels of eell-free DNA and inflammation status in hepatitis C virus-ralated hepatocellular carcinoma.2008

    • Author(s)
      Iida M.
    • Journal Title

      Oncology Reports 20

      Pages: 761-765

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of novel aberrant methylation of BASP1 and SRD5A2 for early diagnosis of hepatocellular carcinoma by genome-wide search.2008

    • Author(s)
      Moribe T.
    • Journal Title

      International Journal of Oncology 33

      Pages: 949-958

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of two isoforms of ubiquitin carboxyl-terminal hydrolase isozyme L1 correlates with high metastatic potentials of human SN12C renal cell carcinoma cell clones.2008

    • Author(s)
      Tanaka T.
    • Journal Title

      Electrophoresis 29

      Pages: 2651-2659

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Translational microarray systems for outcome prediction of hepatocellular careinoma.2008

    • Author(s)
      Iizuka N.
    • Journal Title

      Cancer Science 99

      Pages: 659-665

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ID2 Expression is Associated with a Risk of Hepatocellular Carcinoma Progression.2008

    • Author(s)
      Tsunedomi R.
    • Journal Title

      Clinical Cancer Research 14

      Pages: 1025-1031

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A three-gene predictor for early intrahepatic recurrence of hepatocellular carcinoma after curative hepatectomy.2008

    • Author(s)
      Somura H.
    • Journal Title

      Oncology Report 19

      Pages: 489-495

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CPT-11(Irinotecan)2008

    • Author(s)
      硲 彰一
    • Journal Title

      癌と化学療法 35

      Pages: 182-184

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Circulating cell-free DNA as a predictive marker for distant metastasis of hepatitis C virus-related hepatocellular carcinoma.2007

    • Author(s)
      Tokuhisa Y.
    • Journal Title

      British Journal of Cancer 97

      Pages: 1399-1403

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Geographic bias-based resampling approach reveals a common pathway in early intrahepatic recurrence of hepatocellular carcinoma.2007

    • Author(s)
      Uchimura S.
    • Journal Title

      Anticancer Research 27

      Pages: 3323-3330

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of ID2 associated with invasion of hepatitis C virus-relatedhepatocellular carcinoma by geneexpression profile2006

    • Author(s)
      Tsunedomi R, Iizuka N, Yamada-Okabe H, Tamesa T, Okada T, Sakamoto K, Takashima M, Hamaguchi T, Miyamoto T, Uchimura S, Hamamoto Y, Yamada M, Oka M.
    • Journal Title

      Int J Oncol 29

      Pages: 1445-1451

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of autoantibodies in patients with hepatocellular carcinoma2006

    • Author(s)
      Takashima M, Kuramitsu Y, Yokoyama Y, Iizuka N, Fujimoto M, Sakaida I, Okita K, Oka M, Nakamura K.
    • Journal Title

      Proteomics 6

      Pages: 3894-3900

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Elevated levels of circulating cell-free DNA in the blood of patients with hepatitis C virus-associated hepatocellular carcinoma.2006

    • Author(s)
      Iizuka N
    • Journal Title

      Anticancer Res. 26・6C

      Pages: 4713-4719

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of ID2 associated with invasion of hepatitis C virus-related hepatocellular carcinoma by gene expression profile.2006

    • Author(s)
      Tsunedomi R
    • Journal Title

      Int J Oncol. 29・6

      Pages: 1445-1451

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Molecular features linked to the growth-inhibitory effects of gemcitabine on human pancreatic cancer cells.2006

    • Author(s)
      Toshimitsu M.
    • Journal Title

      Oncol Rep. 16・6

      Pages: 1285-1291

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Different molecular pathways determining extrahepatic and intrahepatic recurrences of hepatocellular carcinoma.2006

    • Author(s)
      Iizuka N.
    • Journal Title

      Oncol Rep. 16・5

      Pages: 1137-1142

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Relation between Cytokine Promoter Gene Polymorphism and Toxicity of 5-Fluorouracil plus Cisplatin Chemotherapy.2006

    • Author(s)
      Sakamoto K.
    • Journal Title

      Oncol Rep. 16・2

      Pages: 381-387

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Proteomic analysis of autoantibodies in patients with hepatocellular carcinoma.2006

    • Author(s)
      Takashima M.
    • Journal Title

      Proteomics. 6・13

      Pages: 3894-3900

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Increased expression and phosphorylation of liver glutamine synthetase in well-differentiated hepatocellular carcinoma tissues from patients infected with hepatitis C virus.2006

    • Author(s)
      Kuramitsu
    • Journal Title

      Electrophoresis 27・8

      Pages: 1651-1658

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Protein level of apolipoprotein E increased in human hepatocellular carcinoma.2006

    • Author(s)
      Yokoyama Y
    • Journal Title

      Int J Oncol 28・3

      Pages: 625-631

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Involvement of c-myc-regulated genes in hepatocellular carcinoma related to genotype-C hepatitis B virus.2006

    • Author(s)
      Iizuka N.
    • Journal Title

      J Cancer Res Clin Oncol. 137・7

      Pages: 473-481

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Esophageal squamous cell carcinomas with distinct invasive depth show different gene expression profiles associated with lymph nodemetasstasis.2006

    • Author(s)
      Sato T.
    • Journal Title

      Int J Oncol. 28・5

      Pages: 1043-1055

    • Related Report
      2006 Annual Research Report
  • [Presentation] Influence of the ID2 on the anti-tumor activity of histone deacetylase inhibitor in hepatocellular carcinoma cells2009

    • Author(s)
      恒富亮一
    • Organizer
      The 19th Conference of the Asian Pacific Association for the Study of the Liver
    • Place of Presentation
      香港
    • Year and Date
      2009-02-14
    • Related Report
      2008 Final Research Report
  • [Presentation] Influence of the ID2 on the anti-tumor activity of histone deacetylase inhibitor in hepatocellular carcinoma cells2009

    • Author(s)
      恒富亮一
    • Organizer
      The 19^<th> Conference of the Asian Pacific Association for the Study of the Liver
    • Place of Presentation
      香港
    • Year and Date
      2009-02-14
    • Related Report
      2008 Annual Research Report
  • [Presentation] Possible new biomarkers for diagnosis of early hepatocellular carcinoma2008

    • Author(s)
      小川恭司
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] HCV陽性肝癌の血清メチル化マーカーとAFP, PIVKA-IIによるハイブリッド高精度診断システム2008

    • Author(s)
      飯塚徳男
    • Organizer
      第7回消化器病フォーム
    • Place of Presentation
      東京
    • Year and Date
      2008-11-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] HCV陽性肝細胞癌の血清Cell-freeDNA量と炎症2008

    • Author(s)
      飯田通久
    • Organizer
      第21回バイオセラピィ学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2008-11-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] 血液検査のハイブリッド化による小肝癌診断2008

    • Author(s)
      飯塚徳男
    • Organizer
      第21回バイオセラピィ学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2008-11-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] 血清メチル化遺伝子と従来マーカーのハイブリット化によるHCV陽性肝癌の高精度早期診断2008

    • Author(s)
      飯塚徳男
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] 肝細胞癌におけるID2 レベルとヒストンデアセチラーゼ阻害剤2008

    • Author(s)
      原田紗和子
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-28
    • Related Report
      2008 Final Research Report
  • [Presentation] 肝細胞癌におけるID2レベルとヒストンデアセチラーゼ阻害剤2008

    • Author(s)
      原田紗和子
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 解糖系モジュールと肝細胞癌(HCC)の進展2008

    • Author(s)
      飯塚徳男
    • Organizer
      第19回日本消化器癌発生学会総会
    • Place of Presentation
      大分
    • Year and Date
      2008-08-29
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Relation of ID2 level to prognosis and metastatic potentials of hepatocellular carcinoma2007

    • Author(s)
      Tsunedomi R.
    • Organizer
      15th European Gastroenrology Week
    • Place of Presentation
      フランス : パリ
    • Year and Date
      2007-10-27
    • Related Report
      2008 Final Research Report
  • [Presentation] Relation of ID2 level to prognosis and metastatic metastatic of hepatocellular carcinoma2007

    • Author(s)
      Tsunedomi R.
    • Organizer
      15th European Gastroentology Week
    • Place of Presentation
      フランス:パリ
    • Year and Date
      2007-10-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] 肝癌細胞の早期肝内再発に対するリアルタイムRT-PCR を応用した予測システムの開発2007

    • Author(s)
      蘇村秀明
    • Organizer
      第45回日本癌治療学会総会
    • Place of Presentation
      京都
    • Year and Date
      2007-10-24
    • Related Report
      2008 Final Research Report
  • [Presentation] 肝癌細胞の早期肝内再発に対するリアルタイムRT-PCRを応用した予測システムの開発2007

    • Author(s)
      蘇村 秀明
    • Organizer
      第45回日本癌治療学会総会
    • Place of Presentation
      京都
    • Year and Date
      2007-10-24
    • Related Report
      2007 Annual Research Report
  • [Presentation] HCV陽性肝細胞癌の遠隔転移規定因子としての血清cell-freeDNA測定意義2007

    • Author(s)
      徳久 善弘
    • Organizer
      第11回日本肝臓学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2007-10-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] HCV関連肝細胞癌におけるHSP70/HLACLass1発現と臨床病理学的因子の検討2007

    • Author(s)
      吉田 晋
    • Organizer
      第20回日本バイオセラピィ学会学術集会総会
    • Place of Presentation
      札幌
    • Year and Date
      2007-10-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] カスタムメイドアレイによる肝癌再発予測2007

    • Author(s)
      飯塚徳男
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2007-10-05
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] ID2 is Associated with Progression of HCV-related Hepatocellar Carcinoma2007

    • Author(s)
      恒富亮一
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2007-10-05
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] リアルタイムRT-PCR 法を用いた肝細胞癌の術後早期肝内再発予測2007

    • Author(s)
      蘇村秀明
    • Organizer
      第62回日本消化器外科学会
    • Place of Presentation
      東京
    • Year and Date
      2007-07-19
    • Related Report
      2008 Final Research Report
  • [Presentation] リアルタイムRT-PCR法を用いた肝細胞癌の術後早期肝内再発予測2007

    • Author(s)
      蘇村 秀明
    • Organizer
      第62回日本消化器外科学会
    • Place of Presentation
      東京
    • Year and Date
      2007-07-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] HCV関連肝細胞癌におけるHSP70およびMHC-Class I発現の検討2007

    • Author(s)
      吉田 晋
    • Organizer
      第62回日本消化器外科学会
    • Place of Presentation
      東京
    • Year and Date
      2007-07-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] 腫瘍の肝転移機序の解明と臨床へのアプローチ2007

    • Author(s)
      吉村 清
    • Organizer
      第62回日本消化器外科学会
    • Place of Presentation
      東京
    • Year and Date
      2007-07-18
    • Related Report
      2007 Annual Research Report
  • [Presentation] HCV陽性肝細胞癌患者の補助診断ツールとしての血清cell-freeDNA2007

    • Author(s)
      友近 忍
    • Organizer
      第16回日本癌病態治療研究会
    • Place of Presentation
      東京
    • Year and Date
      2007-06-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] 肝癌進展における解糖系酸素群の発現パターン2007

    • Author(s)
      濱口卓士
    • Organizer
      第16回日本癌病態治療研究会
    • Place of Presentation
      東京
    • Year and Date
      2007-06-27
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] HCV関連肝細胞癌におけるHSP70およびMHC Class I発現の検討2007

    • Author(s)
      吉田 晋
    • Organizer
      第28回日本癌免疫外科学会
    • Place of Presentation
      東京
    • Year and Date
      2007-05-25
    • Related Report
      2007 Annual Research Report
  • [Presentation] 多群対応Fisher 比を用いた遺伝子選択によるマクロアレイデータの可視化2006

    • Author(s)
      宮本貴宣
    • Organizer
      第65回日本癌学会学術総
    • Place of Presentation
      横浜
    • Year and Date
      2006-09-29
    • Related Report
      2008 Final Research Report
  • [Presentation] ID2 発現はHCV陽性肝細胞癌の門脈浸潤に関連する2006

    • Author(s)
      恒富亮一
    • Organizer
      第65回日本癌学会学術総
    • Place of Presentation
      横浜
    • Year and Date
      2006-09-29
    • Related Report
      2008 Final Research Report
  • [Presentation] 肝細胞癌のプロテオーム解析と臨床応用2006

    • Author(s)
      高島元成
    • Organizer
      日本ヒトプロテオーム機構第4回大会
    • Place of Presentation
      横浜
    • Year and Date
      2006-09-29
    • Related Report
      2008 Final Research Report
  • [Presentation] 肝細胞癌のプロテオーム解析と臨床応用2006

    • Author(s)
      高島元成
    • Organizer
      第61回日本消化器外科学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2006-07-13
    • Related Report
      2008 Final Research Report
  • [Presentation] Dual functions of Eno1 on Vizbility and proliferation of hepatosellular carcinoma cells according to glucose concentration2006

    • Author(s)
      Hamaguchi T.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      京都
    • Year and Date
      2006-06-22
    • Related Report
      2008 Final Research Report
  • [Presentation] ID2 as a possible gene involved in invasion of hapatitis Cvirus-related hepatocellular carcinoma2006

    • Author(s)
      Tsunedomi R.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      京都
    • Year and Date
      2006-06-22
    • Related Report
      2008 Final Research Report
  • [Presentation] 肝細胞ガンにおけるID2発現と転移・浸潤能2006

    • Author(s)
      恒富亮一
    • Organizer
      第42回日本肝臓学会総会
    • Place of Presentation
      京都
    • Year and Date
      2006-05-25
    • Related Report
      2008 Final Research Report
  • [Presentation] ID2 as a possible gene involved in invasion of hapatitis C virus-related hepatocellular carcinoma2006

    • Author(s)
      Tsunedomi R.
    • Organizer
      American Association for Cancer Research 97th Annual Meeting
    • Place of Presentation
      米国
    • Year and Date
      2006-04-03
    • Related Report
      2008 Final Research Report
  • [Presentation] Supervised visualization of microarray date by statistical pattern recognition2005

    • Author(s)
      Miyamato T.
    • Organizer
      American Association for Cancer Research 97th Annual Meeting
    • Place of Presentation
      米国
    • Year and Date
      2005-04-03
    • Related Report
      2008 Final Research Report
  • [Book] 消化器外科学レビュー2002009

    • Author(s)
      吉村清、岡正朗
    • Total Pages
      270
    • Publisher
      総合医学社
    • Related Report
      2008 Final Research Report
  • [Book] 消化器外科レビュー20072008

    • Author(s)
      硲 彰一
    • Total Pages
      260
    • Publisher
      総合医学社
    • Related Report
      2007 Annual Research Report
  • [Book] 消火器外科レビュー20062006

    • Author(s)
      硲 彰一
    • Total Pages
      253
    • Publisher
      総合医学社
    • Related Report
      2006 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi