| Project/Area Number |
18390416
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Okayama University |
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Principal Investigator |
HIROHATA Satoshi Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Department of Molecular Biology and Biochemistry, Assistant Professor (90332791)
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| Co-Investigator(Kenkyū-buntansha) |
NINOMIYA Yoshifumi Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Department of Molecular Biology and Biochemistry, Professor (70126241)
NISHIDA Keiichiro Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Human morphology, Associate Professor (80284058)
NARUSE Keiji Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Cardiovasular physiology, Professor (40252233)
OGAWA Hiroko Okayama University, Medical School, Visiting scientist (70423283)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥17,180,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2007: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2006: ¥9,900,000 (Direct Cost: ¥9,900,000)
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| Keywords | arthritis / aggrecanase / extracellular matrix |
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| Research Abstract |
1 Inducible mechanism for aggrecanase
Chondrocytes were isolated from new born rat knee joint. Mechanical stress was performed and the expressions of aggrecanases were examined. ADAMTS5 was not increased by mechanical stress, while mRNAs of ADAMTS1,4, and ADAMTS9 were increased by mechanical stress. Then, the
2 Distribution of ADAMTS9 in cartilage
We produced osteoarthritis model in Wister rats. When the cartilage destruction was not so severe, the expression of ADAMTS9 was already induced. Then we examined the expression of ADAMTS9 in developmental knee joint. The knee joint was taken at 0, 7, and 14 weeks after birth in ICR mice, respectively. The expression of ADAMTS9 was observed in mature chondrocyte layer (partially) as well as hypertrophic zone. From these experiments, it is suggested that ADAMTS9 is related to the maturation of chondrocytes in knee joint.
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