Research Project
Grant-in-Aid for Scientific Research (B)
1. Analysis of alteration of gene expression after spinal cord injuryGenes of which expression were altered in the injured spinal cord were analyzed by the DNA array method. The 46 genes including inflammatory cytokines or lymphokines were upregulated and 18 genes involving ion transporter or amino acid transporter were downregulated. We focused on and analyzed some genes related to p75NTR signaling.2 Evaluation of the peptides that suppress the p75NTR-induced negative signals.a) A peptide expected to inhibit the binding between RhoGDI and p75, and b) the peptide expected to inhibit the binding between sortilin and pro-brain-derived neurotrophic factor (BDNF) were examined their activities on the locomotion activity after spinal cord injury. However, both peptides failed to improve the locomotion activity. Various proteases induced in the injury site of the spinal cord may be responsible for the degradation of the peptides.3 Amelioration of the spinal cord injury in p75NTR gene-modifie … More d animalsThe improvement of locomotion activity after spinal cord injury was compared between p75NTR gene-disrupted mouse (KO mouse) and wild mouse. KO mouse gave higher scores of the locomotion activity than wild mouse for the first 8 days, but the difference was disappeared thereinafter, suggesting that p75NTR signal disturbs the restoration at early stages of the damage.4 Development of the conditional p75NTR transgenic (Tg) mouseThe vector DNA containing a stop codon between loxp genes and the p75NTR gene in the down stream was constructed, and transferred to fertilized eggs. Because the introduced p75NTR gene does not express as long as stop codon between loxp is not deleted, the maintenance of the animal is easy. If the transgenic mice expressing cre enzyme gene in a region-specicic manner were hybridized with this p75NTR Tg mouse, the p75NTR gene would express simultaneously in the cre enzyme expressing regions. This Tg animals would become useful tool to investigate p75NTR function in the future. Less
All 2008 2007 2006
All Journal Article (51 results) (of which Peer Reviewed: 27 results) Presentation (58 results) Book (1 results)
J Neurosci Res 86
Pages: 1789-1800
Pages: 720-725
Biochem Biophys Res Commun 352
Pages: 360-365
Biochem Biophys Res Commun 356
Pages: 919-924
Biochem Biophys Res Commun 353
Pages: 1056-1062
Biomed Res 28
Pages: 139-146
130004470678
Pages: 261-266
130004903740
Evid Based Complement Altern Med website
Pages: 295-299
Brain and Nerve 59
Pages: 1333-1339
Evid Based Complement Altern Med, Advance Access published on October 29
Evid Based Complement Altern Med (website)
biochem Biophys Res Commun 352
J Neurosci 26
Pages: 3335-3344
Biochem Biophys Res Commun 344
Pages: 941-947
Biosci Biotechnol Biochem 70
Pages: 897-906
Acta Histochem Cytochem 39
Pages: 1-7
J Lipid Res 47
Pages: 1434-1443
J Histochem Cytochem 54
Pages: 1061-1071
Pages: 13218-13230
Chincal Neuroscience(月刊臨床神経科学) 24
Pages: 618-619
110006203385
J Neurosci 26 (51)
Clinical Neuroscience 24 (6)