Amelioration of spinal cord injury by the regulation of neurotmphin receptor p75-mediated signaling

• Project/Area Number: 18390420
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: FURUKAWA Shoei Gifu Pharmaceutical University, Faculty of Pharmaceutical Science, Professor (90159129)
• Co-Investigator(Kenkyū-buntansha): FUKUMITSU Hidefumi Gifu Pharmaceutical University, Faculty of Pharmaceutical Science, Assistant Professor (00308280) ; NOMOTO Hiroshi Gifu Pharmaceutical University, Faculty of Pharmaceutical Science, Associate Professor (80164747) ; FURUKAWA Yoshiko Matsuyama University, Faculty of Pharmaceutical Science, Professor (20219108)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥15,660,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2006: ¥12,800,000 (Direct Cost: ¥12,800,000)
• Keywords: p75NTR / neurotronhin recentor / spinal cord injury / apoptosis / genetically modified animal / recovery of locomotion activity / nerve growth factor / brain-derived neurotrophic factor / p75^<NTR> / 軸索再生 / プロNGF / ソーチリン / DNAアレイ解析

Research Abstract

1. Analysis of alteration of gene expression after spinal cord injury
Genes of which expression were altered in the injured spinal cord were analyzed by the DNA array method. The 46 genes including inflammatory cytokines or lymphokines were upregulated and 18 genes involving ion transporter or amino acid transporter were downregulated. We focused on and analyzed some genes related to p75NTR signaling.
2 Evaluation of the peptides that suppress the p75NTR-induced negative signals.
a) A peptide expected to inhibit the binding between RhoGDI and p75, and b) the peptide expected to inhibit the binding between sortilin and pro-brain-derived neurotrophic factor (BDNF) were examined their activities on the locomotion activity after spinal cord injury. However, both peptides failed to improve the locomotion activity. Various proteases induced in the injury site of the spinal cord may be responsible for the degradation of the peptides.
3 Amelioration of the spinal cord injury in p75NTR gene-modifie d animals
The improvement of locomotion activity after spinal cord injury was compared between p75NTR gene-disrupted mouse (KO mouse) and wild mouse. KO mouse gave higher scores of the locomotion activity than wild mouse for the first 8 days, but the difference was disappeared thereinafter, suggesting that p75NTR signal disturbs the restoration at early stages of the damage.
4 Development of the conditional p75NTR transgenic (Tg) mouse
The vector DNA containing a stop codon between loxp genes and the p75NTR gene in the down stream was constructed, and transferred to fertilized eggs. Because the introduced p75NTR gene does not express as long as stop codon between loxp is not deleted, the maintenance of the animal is easy. If the transgenic mice expressing cre enzyme gene in a region-specicic manner were hybridized with this p75NTR Tg mouse, the p75NTR gene would express simultaneously in the cre enzyme expressing regions. This Tg animals would become useful tool to investigate p75NTR function in the future.

Research Products

• [Journal Article] Adenovirus-mediated retrngrade transfer of neurotrophin-3 gene enhances survival of anterior horn neurons of twy/twy mice with chronic mechanical compression of the spinal cord (2008)
  • Author(s): Uchida K, Nakajima H, Inukai T, Takamura T, Kobayashi S, Furukawa S, Baba H
  • Journal Title: J Neurosci Res 86 Pages: 1789-1800
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Ebselen,a redox regulator containing a selenium atom,induces neurofilament M expression in cultured rat pheochromocytoma PC12 cells via activation of mitogen-activated protein kinase (2008)
  • Author(s): Nishina A, Sekiguchi A, He Y, Koketsu M, Furukawa S
  • Journal Title: J Neurosci Res 86 Pages: 720-725
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Adenovirus-mediated retrograde transfer of neurotrophin-3 gene enhances survival of anterior horn neurons of twy/twy mice with chronic mechanical compression of the spinal cord.- (2008)
  • Author(s): Uchida, K, Nakajima, H, Inukai, T, Takamura, T, Kobayashi, S, Furukawa, S, Baba, H
  • Journal Title: J Neurosci Res 86 Pages: 1789-1800
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ebselen, a redox regulator containing a selenium atom, induces neurofilament M expression in cultured rat pheochromocytoma PC12 cells via activation of mitogen-activated protein kinase. (2008)
  • Author(s): Nishina, A, Sekiguchi, A, He, Y, Koketsu, M, Furukawa, S
  • Journal Title: J Neurosci Res 86 Pages: 720-725
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Adenovirus-mediated retrograde transfer of neurotrophin-3 gene enhances survival of anterior horn neurons of twy/twy mice with chronic mechanical compression of the spinal cord. (2008)
  • Author(s): Uchida K, Nakajima H, Inukai T, Takamura T, Kobayashi S, Furukawa S, Baba H
  • Journal Title: J Neurosci Res 86 Pages: 1789-1800
  • Peer Reviewed
• [Journal Article] Ebselen, a redox regulator containing a selenium atom, induces neurofilament Mexpression in cultured rat pheochromocytoma PC12 cells via activation of mitogen-activated protein kinase. (2008)
  • Author(s): Nishina A, Sekiguchi A, He Y, Koketsu M, Furukawa S
  • Journal Title: J Neurosci Res 86 Pages: 720-725
  • Peer Reviewed
• [Journal Article] Selenazoles(selenium compounds)facilitate survival of cultured rat pheochfomocytoma PC12 cells after serum-deprivation and stimulate their neuronal differentiation via activation of Akt and mitogen-activated protem kinase,respectively (2007)
  • Author(s): Nishina A, Sekiguchi A, Fukumoto R, Koketsu M, Furukawa S
  • Journal Title: Biochem Biophys Res Commun 352 Pages: 360-365
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Pro-region of neurotrophins determines the processing efficiency (2007)
  • Author(s): Nomoto H, Takaiwa M, Mouri A, Furukawa S
  • Journal Title: Biochem Biophys Res Commun 356 Pages: 919-924
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Processing of nerve growth factor:The role of basic amino acid clusters in the pro-region (2007)
  • Author(s): Mouri A, Nomoto H, Furukawa S
  • Journal Title: Biochem Biophys Res Commun 353 Pages: 1056-1062
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Royal jelly-induced neurite outgrowth from rat pheochromocytoma PC12 cells requires integrin signal independent of activation of extracellular signal-regulated kinases (2007)
  • Author(s): Hattori N, Nomoto H, Fukumitsu H, Mishma S, Furukawa S
  • Journal Title: Biomed Res 28 Pages: 139-146
  • NAID: 130004470678
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Royal jelly and its unique fatty acid,10-hydroxy-trans-2-decenoic acid,promote neurogenesis by neural stem/progenitor cells in vitro (2007)
  • Author(s): Hattori N, Nomoto H, Fukumitsu H, Mishma S, Furukawa S
  • Journal Title: Biomed Res 28 Pages: 261-266
  • NAID: 130004903740
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] AMP N_1-oxide,a unique compound of royal jelly,induces neurite outgrowth from PC12 cells via signaling by protein kinase A independent of that by mitogen-activated protein kinase (2007)
  • Author(s): Hattori N, Nomoto H, Fukumitsu H, Mishima S, Furukawa S
  • Journal Title: Evid Based Complement Altern Med website
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] AMP N_1-oxide potentiates astrogenesis by cultured neural stem/progenitor cells through STAT3 activation (2007)
  • Author(s): Hattori N, Nomoto H, Fukumitsu H, Mishma S, Furukawa S
  • Journal Title: Biomed Res 28 Pages: 295-299
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 脊髄損傷修復と神経栄養因子-FGF-2に注目して- (2007)
  • Author(s): 古川 昭栄, 古川 美子
  • Journal Title: Brain and Nerve 59 Pages: 1333-1339
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Selenazoles (selenium compounds) facilitate survival of cultured rat pheochromocytoma PC12 cells after serum-deprivation and stimulate their neuronal differentiation via activation of Akt and mitogen-activated protein kinase, respectively (2007)
  • Author(s): Nishina, A, Sekiguchi, A, Fukumoto, R, Koketsu, M, Furukawa, S
  • Journal Title: Biochem Biophys Res Commun 352 Pages: 360-365
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Pro-region of neurotronhins determines the nrocessine efficiency_ (2007)
  • Author(s): Nomoto, H, Takaiwa, M, Mouri, A, Furukawa, S
  • Journal Title: Biochem Biophys Res Commun 356 Pages: 919-924
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Processing of nerve growth factor: The role of basic amino acid clusters in the pro-region (2007)
  • Author(s): Mouri, A, Nomoto, H, Furukawa, S
  • Journal Title: Biochem Biophys Res Commun 353 Pages: 1056-1062
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Royal jelly-induced neurite outgrowth from rat pheochromocytoma PC12 cells requires integrin signal independent of activation of extracellular signal-regulated kinases. (2007)
  • Author(s): Hattori, N, Nomoto, H, Fukumitsu, H, Mishima, S, Furukawa, S
  • Journal Title: Biomed Res 28 Pages: 139-146
  • NAID: 130004470678
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Royal jelly and its unique fatty acid, 10-hydroxy-trans-2-decenoic acid, promote neurogenesis by neural stem/progenitor cells in vitro. (2007)
  • Author(s): Hattori, N, Nomoto, H, Fukumitsu, H, Mishima, S, Furukawa, S
  • Journal Title: Biomed Res 28 Pages: 261-266
  • NAID: 130004903740
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] AMP N_1-oxide, a unique compound of royal jelly, induces neurite outgrowth from PC12 cells via signaling by protein kinase A independent of that by mitogen-activated protein kinase (2007)
  • Author(s): Hattori, N, Nomoto, H, Fukumitsu, H, Mishima, S, Furukawa, S
  • Journal Title: Evid Based Complement Altern Med, Advance Access published on October 29
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] AMP N-oxide potentiates astrogenesis by cultured neural stem/progenitor cells through STAT3 activation. (2007)
  • Author(s): Hattori, N, Nomoto, H, Fukumitsu, H, Mishima, S, Furukawa, S
  • Journal Title: Biomed Res 28 Pages: 295-299
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Roles of neurotrophic factors in regeneration of spinal cord injury. Focus on FGF-2 (2007)
  • Author(s): Furukawa, S, Furukawa, Y
  • Journal Title: Brain and Nerve 59 Pages: 1333-1339
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Pro-region of neurotrophins determines the processing efficiency. (2007)
  • Author(s): Nomoto H, Takaiwa M, Mouri A, Furukawa S
  • Journal Title: Biochem Biophys Res Commun 356 Pages: 919-924
  • Peer Reviewed
• [Journal Article] Processing of nerve growth factor: The role of basic amini acid clusters in the pro-region. (2007)
  • Author(s): Mouri A, Nomoto H, Furukawa S
  • Journal Title: Biochem Biophys Res Commun 353 Pages: 1056-1062
  • Peer Reviewed
• [Journal Article] Royal jelly-induced neurite outgrowth from rat pheochromocytome PC12 cells requires integrin signal independent of activation of extracellular signal-regulated kinases. (2007)
  • Author(s): Hattori N, Nomoto H, Fukumitsu H, Mishima S, Furukawa S
  • Journal Title: Biomed Res 28 Pages: 139-146
  • Peer Reviewed
• [Journal Article] Royal jelly and its unique fatty acid, 10-hydroxy-trans-2-decenoic acid, promote neurogenesis by neural stem/progenitor cells in vitro. (2007)
  • Author(s): Hattori N, Nomoto H, Fukumitsu H, Mishima S, Furukawa S
  • Journal Title: Biomed Res 28 Pages: 261-266
  • NAID: 130004903740
  • Peer Reviewed
• [Journal Article] AMP N_1-oxide, a unique compound of royal jelly, induces neurite outgrowth from PC12 cells via signaling by protein kinase A independent of that by mitogen-activated protein kinase. (2007)
  • Author(s): Hattori N, Nomoto H, Fukumitsu H, Mishima S, Furukawa S
  • Journal Title: Evid Based Complement Altern Med (website)
  • Peer Reviewed
• [Journal Article] AMP N_1-oxide potentiates astrogenesis by cultured neural stem/progenitor cells through STAT3 activation. (2007)
  • Author(s): Hattori N, Nomoto H, Fukumitsu H, Mishima S, Furukawa S
  • Journal Title: Biomed Res 28 Pages: 295-299
  • Peer Reviewed
• [Journal Article] 脊髄損傷修復と神経栄養因子-FGF-2に注目して- (2007)
  • Author(s): 古川昭栄, 古川美子
  • Journal Title: Brain and Nerve 59 Pages: 1333-1339
  • Peer Reviewed
• [Journal Article] Processing of nerve growth factor : The role of basic amino acid clusters in the pro-region. (2007)
  • Author(s): Mouri A, Nomoto H, Furukawa S.
  • Journal Title: Biochem Biophys Res Commun 353 Pages: 1056-1062
• [Journal Article] selenazoles (selenium compounds) facilitate survival of cultured rat pheochromocytoma PC12 cells after serum-deprivation and stimulate their neuronal differentiation via activation of Akt and mitogen-activated protein kinase, respectively. (2007)
  • Author(s): Nishina A, Sekiguchi A, Fukumoto R, Koketsu M, Furukawa S.
  • Journal Title: biochem Biophys Res Commun 352 Pages: 360-365
• [Journal Article] An analog of a dipeptide-like structure of FK506 increases glial cell line-derived neurotrophic factor expression through cAMP response element-binding protein activated by heat shock protein 90/Akt signaling pathway (2006)
  • Author(s): Cen X, Nitta A, Ohya S, Zhao Y, Ozawa N, Mouri A, Wang L, Suzuki M, Saito K, Ito Y, Kawagoe T, Noda Y, Ito Y, Furukawa S, Nabesbima T
  • Journal Title: J Neurosci 26 Pages: 3335-3344
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Autocrine activation of cultured macrophages by brain-derived neurotrophic factor (2006)
  • Author(s): Asami T, Ito T, Fukumitsu K, Nomoto H, Furukawa Y, Furukawa S
  • Journal Title: Biochem Biophys Res Commun 344 Pages: 941-947
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Identification of AMP N_1-oxide in royal jelly as a neurotrophic component for cultured rat pheochromocytoma PC12 cells (2006)
  • Author(s): Hattori N, Nomoto H, Mishima S, Inagaki S, Goto M, Sako M, Furukawa S
  • Journal Title: Biosci Biotechnol Biochem 70 Pages: 897-906
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Comparison of FGF1(aFGF)expression between the dorsal motor nucleus ofvagus and the hypoglossal nucleus of rat (2006)
  • Author(s): Toyoda K, OkanoH, Bamba H, Hisa Y, Oomura Y, Imamura T, Furukawa S, Tooyama I
  • Journal Title: Acta Histochem Cytochem 39 Pages: 1-7
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Lysophosphatidylethanolamine in Grifola frondosa as a neurotrophic activator via activation of MAP kinase (2006)
  • Author(s): Nishna A, Kimura H, Sekiguchi A, Fukumoto R, Nakajima S, Furukawa S
  • Journal Title: J Lipid Res 47 Pages: 1434-1443
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Localization of fibroblast growth factor-1 in cholinergic neurons innervating the rat larynx (2006)
  • Author(s): Okano H, Toyoda K, Bamba H, Hisa Y, Oomura Y, Imamura T, Furukawa S, Kimura H, Tooyama I
  • Journal Title: J Histochem Cytochem 54 Pages: 1061-1071
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Brain-derived neurotrophic factor participates in determination of neuronal laminar fate in the developing mouse cerebral cortex (2006)
  • Author(s): Fukumitsu H, Ohtsuka M, Murai R, Nakamura H, Itch K, Furukawa S
  • Journal Title: J Neurosci 26 Pages: 13218-13230
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 神経保護作用薬(7)神経栄養因子によるニューロン死の制御 (2006)
  • Author(s): 古川 昭栄, 古川 美子
  • Journal Title: Chincal Neuroscience(月刊臨床神経科学) 24 Pages: 618-619
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] An analog of a dipeptide-like structure of FK506 increases glial cell line-derived neurotrophic factor expression through cAMP response element-binding protein activated by heat shock protein 90/Akt signaling pathway. (2006)
  • Author(s): Cen, X, Nitta, A, Ohya, S, Zhao, Y, Ozawa, N, Mouri, A, Wang, L, Suzuki ,M, Saito, K, Ito, Y, Kawagoe, T, Noda, Y, Ito, Y, Furukawa, S, Nabeshima, T
  • Journal Title: J Neurosci 26 Pages: 3335-3344
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Autocrine activation of cultured macrophages by brain-derived neurotrophic factor. (2006)
  • Author(s): Asami, T, Ito, T, Fukumitsu, H., Nomoto, H., Furukawa, Y., Furukawa, S
  • Journal Title: Biochem Biophys Res Commun 344 Pages: 941-947
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of AMP N_1-oxide in royal jelly as a neurotrophic component for cultured rat pheochromocytoma PC12 cells (2006)
  • Author(s): Hattori, N, Nomoto, H, Mishima, S, Inagaki, S, Goto, M, Sako, M, Furukawa S
  • Journal Title: Biosci Biotechnol Biochem 70 Pages: 897-906
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparison of FGF1 (aFGF) expression between the dorsal motor nucleus of vagus and the hypoglossal nucleus of rat (2006)
  • Author(s): Toyoda, K, Okano, H, Bamba, H, Hisa, Y, Oomura, Y, Imamura, T, Furukawa, S, Tooyama, I
  • Journal Title: Acta Histochem Cytochem 39 Pages: 1-7
  • NAID: 110006203385
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Lysophosphatidylethanolamine in Grifola frondosa as a neurotrophic activator via activation of MAP kinase. (2006)
  • Author(s): Nishina, A, Kimura, H, Sekiguchi, A, Fukumoto, R, Nakajima, S, Furukawa, S
  • Journal Title: J Lipid Res 47 Pages: 1434-1443
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Localization of fibroblast growth factor-1 in cholinergic neurons innervating the rat larynx. (2006)
  • Author(s): Okano, H, Toyoda, K, Bamba, H, Hisa, Y, Oomura, Y, Imamura, T, Furukawa, S, Kimura. H, Tooyama, I
  • Journal Title: J Histochem Cytochem 54 Pages: 1061-1071
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Brain-derived neurotrophic factor participates in determination of neuronal laminar fate in the developing mouse cerebral cortex. (2006)
  • Author(s): Fukumitsu, H, Ohtsuka, M, Murai, R, Nakamura, H, Itoh, K, Furukawa, S
  • Journal Title: J Neurosci 26 (51) Pages: 13218-13230
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Drugs with neuroprotective activity (7) Protection against neuronal death by neurotrophic factors. (2006)
  • Author(s): Furukawa, S, Furukawa, Y
  • Journal Title: Clinical Neuroscience 24 (6) Pages: 618-619
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Brain-derived neurotrophic factor participates in determination of neuronal laminar fate in the developing mouse cerebral cortex. (2006)
  • Author(s): Fukumitsu H, Ohtsuka M, Murai R, Nakamura H, Itoh K, Shoei Furukawa.
  • Journal Title: J Neurosci 26 Pages: 13218-13230
• [Journal Article] Lysophosphatidylethanolamine in Grifola frondosa as a neurotrophic activator via activation of MAP kinase. (2006)
  • Author(s): Nishina A, Kimura H, Sekiguchi A, Fukumoto R, Nakajima S, Furukawa S.
  • Journal Title: J Lipid Res 47 Pages: 1434-1443
• [Journal Article] Identification of AMP N_1-oxide in royal jelly as a neurotrophic component for cultured rat pheochromocytoma OC12 cells. (2006)
  • Author(s): Hattori N, Nomoto H, Mishima S, Inagaki S, Goto M, Sako M, Furukawa S.
  • Journal Title: Biosci Biotechnol Biochem 70 Pages: 897-906
• [Journal Article] Autocrine activation of cultured macrophages by brain-derived neurotrophic factor. (2006)
  • Author(s): Asami T, Ito T, Fukumitsu H, Nomoto H, Furukawa Y, Furukawa S.
  • Journal Title: Biochem Biophys Res Commun 344 Pages: 941-947
• [Presentation] 脳内MAPキナーゼの活性化と抗うつ作用 (2008)
  • Author(s): 牧野 晃久、福光 秀文、古川 昭栄
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 4・メチルカテコールによる神経幹細胞保護作用 (2008)
  • Author(s): 古川 美子、浦野 友美、南村 美里、中島 光業、加来 鉄平、古川 昭栄
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] A relationship between activation of MAP kinase of the brain and anti-depression effect (2008)
  • Author(s): Makino, A, Fukumitsu, H, Furukawa, S
  • Organizer: The 128th Annual Meeting of The Pharmaceutical Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Protective effect of 4-methylcatechol against cell death of neural stem cells (2008)
  • Author(s): Furukawa, Y, Urano, T, Minamimura, M, Nakajima, M, Kaku, T, Furukawa S
  • Organizer: The 128th Annual Meeting of The Pharmaceutical Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Amelioration of the ability of memory and learning by royal jelly (2008)
  • Author(s): Hattori, N, Ohta, S, Sakamoto, T, Furukawa, S, Mishima, S
  • Organizer: The 128th Annual Meeting of The Pharmaceutical Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 4-メチルカテコールによる神経幹細胞保護作用 (2008)
  • Author(s): 古川美子、浦野友美、南村美里、中島光業、加来鉄平、古川昭栄
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-27
• [Presentation] ローヤルゼリーのマウス学習記憶障害改善作用 (2008)
  • Author(s): 服部徳子、大田象三、坂本貴、古川昭栄、三島敏
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-27
• [Presentation] セレンを分子中に含有するレドックス抑制剤エブセレンは、PC12細胞のマップキナーゼの活性化を介してニューロフィラメントMの産生を亢進する (2007)
  • Author(s): 仁科 淳良、関口 昭博、纐纈 守、古川 昭栄
  • Organizer: 第80回日本生化学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 生合成された神経成長因子(NGF)と受容体の細胞内での相互作用 (2007)
  • Author(s): 野元 裕、浴永 圭吾、加藤 由佳、古川 昭栄
  • Organizer: 第80回日本生化学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Ebselen, a redox regulator containing a selenium atom, induces neurofilament M expression in cultured rat pheochromocytoma PC12 cells via activation of mitogen-activated protein kinase (2007)
  • Author(s): Nishina, A., Sekiguchi, A., Koketsu, M., Furukawa, S
  • Organizer: The 80th Annual Meeting of the ` Japanese Biochemical Society
  • Place of Presentation: Yokohama
  • Year and Date: 2007-12-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Intracellular interaction between synthesized nerve growth factor and its receptors (2007)
  • Author(s): Nomoto, H, Ekinaga, K, Kato, Y, Furukawa, S
  • Organizer: The 80th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Yokohama
  • Year and Date: 2007-12-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] マウス大脳皮質構築過程の神経細胞層形成に対するニュートロフィン・3の影響 (2007)
  • Author(s): 大塚 正成、福光 秀文、古川 昭栄
  • Organizer: 第50回日本神経化学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] BDNFによる神経幹細胞から分化した神経前駆細胞への生存保護効果 (2007)
  • Author(s): 久戸瀬 哲也、福光 秀文、古川 昭栄
  • Organizer: 第50回日本神経化学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 大脳皮質形成過程における幹細胞因子の役割 (2007)
  • Author(s): 宗宮 仁美、福光 秀文、国貞 隆弘、古川 昭栄
  • Organizer: 第50回日本神経化学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] NMDA誘発マウス網膜障害モデルにおける外側外状体の経時的部位的変化 (2007)
  • Author(s): 伊藤 保志、嶋澤 雅光、井口 勇太、福光 秀文、古川 昭栄、新家 眞、原英 彰
  • Organizer: 第50回目本神経化学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effect of neurotrophin-3 on the neuronal laminar formation during development of the mouse cerebral cortex (2007)
  • Author(s): Otsuka, M, Fukumitsu, H, Furukawa, S
  • Organizer: The 50th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: Yokohama
  • Year and Date: 2007-09-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Survival promoting activity by BDNF of neuronal progenitors differentiated from neural stem cell (2007)
  • Author(s): Kudose, T, Fukumitsu, H, Furukawa, S
  • Organizer: The 50th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: Yokohama
  • Year and Date: 2007-09-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Roles of stem cell factor on the development of the cerebral cortex (2007)
  • Author(s): Soumiya, H, Fukumitsu, H, Kunisada, T, Furukawa, S
  • Organizer: The 50th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: Yokohama
  • Year and Date: 2007-09-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Araie M, Hara H. A time-dependent change in the lateral genuculate body of animal model of the NMDA-induced degeneration of the retina (2007)
  • Author(s): Ito, Y, Shimazawa, M, Inokuchi, Y, Fukumitsu, H, Furukawa, S
  • Organizer: The 50th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: Yokohama
  • Year and Date: 2007-09-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] マウス大脳皮質構築過程の神経細胞層形成に対するニュートロフィン-3の影響 (2007)
  • Author(s): 大塚正成、福光秀文、古川昭栄
  • Organizer: 第50回日本神経化学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
• [Presentation] NMDA誘発マウス網膜障害モデルにおける外側外状体の経時的部位的変化 (2007)
  • Author(s): 伊藤保志、嶋澤雅光、井口勇太、福光秀文、古川昭栄、新家眞、原英彰
  • Organizer: 第50回日本神経化学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
• [Presentation] 脳由来神経栄養因子による大脳皮質神経細胞の層特異的性質の決定 (2007)
  • Author(s): 大塚 正成、福光 秀文、古川 昭栄
  • Organizer: 神経組織の成長・再生・移植研究会
  • Place of Presentation: 岡山
  • Year and Date: 2007-05-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Decision of laminar fate of the cerebral cortex by brain-derived neurotrophic factor (2007)
  • Author(s): Otsuka, M, Fukumitsu, H, Furukawa, S
  • Organizer: The 22th Annual Meeting of the Japanese Society for Neural Growth, Regeneration and Plantation
  • Place of Presentation: Okayama
  • Year and Date: 2007-05-26
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 疎水性ジペプチドによる神経栄養因子誘導と神経疾患治療薬への可能性 (2007)
  • Author(s): 新田 淳美, 古川 昭栄, 鍋島 俊隆
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山
  • Year and Date: 2007-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 神経栄養因子(NGF)の細胞内での作用について (2007)
  • Author(s): 野元 裕, 加藤 白佳, 野津 尚子, 古川 昭栄
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山
  • Year and Date: 2007-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] マウス大脳皮質形成過程の層形成に及ぼすニュートロフィン-3の作用 (2007)
  • Author(s): 大塚 正成, 福光 秀文, 古川 昭栄
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山
  • Year and Date: 2007-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 培養神経幹細胞に及ぼすエストロゲンの影響 (2007)
  • Author(s): 古川 美子, 岡田 牧子, 中島 光業, 古川 昭栄
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山
  • Year and Date: 2007-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 増殖中の神経系前駆細胞の性状に及ぼす脳由来神経栄養因子の作用 (2007)
  • Author(s): 福光 秀文, 坂井 美香, 久戸瀬 哲也, 古川 昭栄
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山
  • Year and Date: 2007-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Induction of neurotrophic factors by a hydrophobic dipeptide and its possible application to the neurological disorders as a therapeutic reagent (2007)
  • Author(s): Nitta, A, Furukawa, S, Nabeshima, T
  • Organizer: The 127th Annual Meeting of The Pharmaceutical Society of Japan
  • Place of Presentation: Toyama
  • Year and Date: 2007-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Intracellular action of nerve growth factor (2007)
  • Author(s): Nomoto, H, Katoh, Y, Notsu, H, Furukawa, S
  • Organizer: The 127th Annual Meeting of The Pharmaceutical Society of Japan
  • Place of Presentation: Toyama
  • Year and Date: 2007-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Decision of laminar fate of the cerebral cortex by neurotronhin-3- (2007)
  • Author(s): Otsuka, M, Fukumitsu, H, Furukawa, S
  • Organizer: The 127th Annual Meeting of The Pharmaceutical Society of Japan
  • Place of Presentation: Toyama
  • Year and Date: 2007-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effects of estrogens on cultured neural stem/progenitor cells (2007)
  • Author(s): Furukawa, Y, Okada, M, Nakajima, M, Furukawa, S
  • Organizer: The 127th Annual Meeting of The Pharmaceutical Society of Japan
  • Place of Presentation: Toyama
  • Year and Date: 2007-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effects of brain-derive dneurotrophic factor on proliferating neural stem/orogenitor cells (2007)
  • Author(s): Fukumitsu, H, Sakai, M, Kudose, T, Furukawa, S
  • Organizer: The 127th Annual Meeting of The Pharmaceutical Society of Japan
  • Place of Presentation: Toyama
  • Year and Date: 2007-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ローヤルゼリーのマウス学習記憶障害改善作用 (2007)
  • Author(s): 服部 徳子、大田 象三、坂本 貴、古川 昭栄、三島 敏
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-03-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Actions of royal jelly on nervous system (2007)
  • Author(s): Furukawa S
  • Organizer: The second International Symposium of Japanese Apitherapy Society
  • Place of Presentation: Narita,Japan
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Regenerative actions of Royal jelly on nervous system (2007)
  • Author(s): Furukawa S
  • Organizer: The first Romanian Apitherapy Society Symposium, Workshop and Expo with lnternational Participation
  • Place of Presentation: Cluj-Napoca,Romania
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Actions of royal jelly on nervous system (2007)
  • Author(s): Furukawa, S
  • Organizer: The second International Symposium of Japanese Apitherapy Society
  • Place of Presentation: Narita, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Regenerative actions of Royal jelly on nervous system (2007)
  • Author(s): Furukawa, S
  • Organizer: The first Romanian Apitherapy Society Symposium, Workshop and Expo with International Participation
  • Place of Presentation: Cluj-Napoca, Romania
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Actions of royal jelly on nervous system. (2007)
  • Author(s): Furukawa S
  • Organizer: The second International Symposium of Japanese Apitherapy Society
  • Place of Presentation: Narita, Japan
• [Presentation] Regenerative actions of Royal jelly on nervous system. (2007)
  • Author(s): Furukawa S
  • Organizer: The first Romanian Apitherapy Society Symposium, Workshop and Expo with International Participation
  • Place of Presentation: Cluj-Napoca, Romania
• [Presentation] Brain-derived neurotrophic factor-dependent regulation of survival and/or proliferation of cortical progenitor cells (2006)
  • Author(s): Fukumitsu H, Sakai M, Kudose T, Furukawa S
  • Organizer: Annual neuroscience meeting 2006
  • Place of Presentation: Atlanta
  • Year and Date: 2006-10-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] BDNF influences the cortical laminar fate decision during development of the mouse cerebral cortex (2006)
  • Author(s): Otsuka M, Fukumitsu H, Furukawa S
  • Organizer: Annual neuroscience meeting 2006
  • Place of Presentation: Atlanta
  • Year and Date: 2006-10-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Brain-derived neurotrophic factor-dependent regulation of survival and/or proliferation of cortical progenitor cells (2006)
  • Author(s): Fukumitsu, H, Sakai, M, Kudose, T, Furukawa, S
  • Organizer: Annual neuroscience meeting
  • Place of Presentation: Atlanta
  • Year and Date: 2006-10-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] BDNF influences the cortical laminar fate decision during development of the mouse cerebral cortex (2006)
  • Author(s): Otsuka, M, Fukumitsu, H, Furukawa, S
  • Organizer: Annual neuroscience meeting
  • Place of Presentation: Atlanta
  • Year and Date: 2006-10-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Leu-lle increases GDNF through CREB activation by Hsp90/Akt signaling pathway (2006)
  • Author(s): 新田 淳美, Cen Xiaobo, 大谷 晋, 小沢 直哉, 丹羽 美苗, 古川 昭栄, 鍋島 俊隆
  • Organizer: 第49回日本神経化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Neurotrophin-3 increases cortical progenitor cells during laminlar formation (2006)
  • Author(s): 大塚 正成, 福光 秀文, 古川 昭栄
  • Organizer: 第49回日本神経化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Royal jelly enhances neuronal differentiation of cultured neural stem cells (2006)
  • Author(s): 服部 徳子, 荒木 陽子, 坂本 貴, 三島 敏, 野元 裕, 古川 昭栄
  • Organizer: 第49日本神経化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Mechanism of the protective action of 4-methylcatechol on cultured neural stem cells (2006)
  • Author(s): 浦野 友美, 古川 美子, 古川 昭栄
  • Organizer: 第49回日本神経化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] BDNF-dependent regulation of survival and/or proliferation of cortical neural stem/progenitor cells (2006)
  • Author(s): 福光 秀文, 坂井 美香, 古川 昭栄
  • Organizer: 第49回目本神経化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Identification of lysophosphatidylethanolamine in grifola frondosa as a molecule with neuroprotective and/or neurotrophic activities toward cultured PC12 cells via activation ofMAP kinase (2006)
  • Author(s): Nishina A, Kimura H, Sekiguchi A, Fuklumoto R, Nakajima S, Furukawa S
  • Organizer: 第79回日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2006-06-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Identification of lysophosphatidylethanolamine in grifola frondosa as a molecule with neuroprotective and/or neurotrophic activities toward cultured PC12 cells via activation of MAP kinase. (2006)
  • Author(s): Nishina, A, Kimura, H, Sekiguchi, A, Fukumoto, R, Nakajima, S, Furukawa, S
  • Organizer: The 79th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 脳由来神経栄養因子による神経前駆細胞への分化誘導とその生存効果 (2006)
  • Author(s): 福光 秀文, 坂井 美香, 古川 昭栄
  • Organizer: 神経組織の成長・再生・移植研究会第21回学術集会
  • Place of Presentation: 東京
  • Year and Date: 2006-05-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Stimulation of differentiation from neural stem cells to neuronal progenitor cells and maintenance of their survival by brain-derived neurotrophic factor. (2006)
  • Author(s): Fukumitsu, H, Sakai, M., Furukawa, S
  • Organizer: The 21th Annual Meeting of the Japanese Society for Neural Growth, Regeneration and Plantation
  • Place of Presentation: Tokyo
  • Year and Date: 2006-05-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Leu-lle increases GDNF through CREB activation by Hsp90/Akt signaling pathway. The 49th Annual Meeting of the Japanese Society for Neurochemistry (2006)
  • Author(s): Nitta, A, Cen Xiaobo, Ohtani, S, Ozawa, N, Niwa, M, Furukawa, S, Nabeshima, T
  • Organizer: The 49th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: Nagoya
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Neurotrophin-3 increases cortical progenitor cells during laminar formation. (2006)
  • Author(s): Ohtsuka, M, Fukumitsu, H, Furukawa, S
  • Organizer: The 49th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: Nagoya
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Royal jelly enhances neuronal differentiation of cultured neural stem cells (2006)
  • Author(s): Hattori, N, Araki, Y, Sakamoto, T, Mishima, S, Nomoto, H, Furukawa, S
  • Organizer: The 49th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: Nagoya
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Mechanism of the protective action of 4-methvlcatechol on cultured neural stem cells. (2006)
  • Author(s): Urano, T, Furukawa, Y, Furukawa, S
  • Organizer: The 49th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: Nagoya
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] BDNF-dependent regulation of survival and/or proliferation of cortical neural stem/progenitor cells (2006)
  • Author(s): Fukumitsu, H, Sakai, M, Furukawa, S
  • Organizer: The 49th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: Nagoya
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 神経成長因子(NGF)と神経保護作用.細胞増殖因子と再生医療松本邦夫、田畑泰彦監修 (2006)
  • Author(s): 古川 昭栄, 古川 美子
  • Publisher: メジカルレビュー
  • Description: 「研究成果報告書概要(和文)」より