• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on the role of lysophosphatidic acid in the seminal fluids and its related molecules in the development of prostate cancer

Research Project

Project/Area Number 18390438
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionKagawa University

Principal Investigator

KAKEHI Yoshiyuki  Kagawa University, Faculty of Medicine, Professor (20214273)

Co-Investigator(Kenkyū-buntansha) WU Xiuxian  Kagawa University, Faculty of Medicine, Associate (10346645)
TAOKA Rikiya  Kagawa University, Faculty of Medidne, Associate (10403784)
INUI Mashashi  Kagawa University, Faculty of Medicine, Assistant Professor (40314918)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥12,290,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2007: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2006: ¥8,000,000 (Direct Cost: ¥8,000,000)
KeywordsLysophosohatidic acid (LPA) / Prostate cancer / Benign prostate hyperplasia / Autotaxin / Acylglycerol kinase / Lipid mediator / Acylglycerol Kinase / リゾフォスファチジン酸 / lyso PLD / 前立腺癌 / アシルグリセロールキナー / 精漿
Research Abstract

This study was carried out to elucidate the role of lysophosphatidic acid (LPA) and LPA-relating molecules in the development and progression of prostate cancer. Prostate tissue samples were surgically obtained from 10 patients with localized prostate cancer and 7 patients with invasive bladder cancer. Cancer cells, high grade intraepithelial neoplasia (HGPIN), benign hyperplastic glands, normal glands, and their corresponding stromal cells were isolated by laser capture microdissection. Normal glands and its corresponding stroma from the prostate of bladder cancer patients were referred as a control. mRNA levels of three LPA receptors, LPA1, LPA2, LPA3, two LPA-synthetic enzymes, autotaxin (ATX), acylglycerol kinase (AGK), and a LPA-degradation enzyme, prostatic acid phosphatase (PAP), were quantitatively assessed. Gene expression levels were also evaluated in human prostate cancer LNCaP, PC-3, and DU-145 cells.LPA1 mRNA level was significantly decreased in HGPIN and cancer epithelia as compared to the benign glands. LPA3 mRNA level was elevated in cancer epithelia as compared to control benign glands. LPA3, AGK, and PAP gene expressions were predominant in LNCaP cells while LPA1 and ATX gene expressions were predominant in PC-3 and Du-145 cells. In BPH, elevated expression of AGK in stroma and PAP in epithelia was simultaneously found. LPA stimuli via LPA3 may play a pivotal role in the development of prostate cancer. Switching of predominant type of LPA receptors (LPA3 to LPA1) may be involved in the progression and/or androgen independence.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (16 results)

All 2008 2007 2006

All Journal Article (13 results) (of which Peer Reviewed: 4 results) Presentation (3 results)

  • [Journal Article] Complication rates of ultrasound-guided prostate biopsy:a nation-wide survey in Japan2008

    • Author(s)
      Kakehi Y, ほか
    • Journal Title

      International Journal of Urology 15

      Pages: 319-321

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Prospective evaluation of selection criteria for active surveillance in Japanese patients with stage T1cN0M0 prostate cancer2008

    • Author(s)
      Kakehi Y, ほか
    • Journal Title

      Japanese Journal of Clinical Oncology 38

      Pages: 122-128

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Complication rates of ultrasound-guided prostate biopsy : a nation-wide survey in Japan2008

    • Author(s)
      Kakehi, Y, et. al.
    • Journal Title

      International Journal of Urology 15

      Pages: 319-321

    • NAID

      10021277523

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Prospective evaluation of selection criteria for active surveillance in Japanese patients with stage T1cN0M0 prostate cancer2008

    • Author(s)
      Kakehi, Y, et. al.
    • Journal Title

      Japanese Journal of Clinical Oncology 38

      Pages: 122-128

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Complication rates of ultrasound guided prostate biopsy a nation-wide survey in Japan2008

    • Author(s)
      Kakehi Y, ほか
    • Journal Title

      International Journal of Urology 15

      Pages: 319-321

    • NAID

      10021277523

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Health related quality of life in Japanese men with localized prostate cancer treated with current multiple modalities assessed by a newly developed Japanese version of the Expanded Prostate Cancer Index Composite2007

    • Author(s)
      Kakehi Y, ほか
    • Journal Title

      Journal of Urology 177

      Pages: 1856-1861

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Health related quality of life in Japanese men with localized prostate cancer treated with current multiple modalities assessed by a newly developed Japanese version of the Expanded Prostate Cancer Index Composite2007

    • Author(s)
      Kakehi, Y, et. al.
    • Journal Title

      Journal of Urology 177

      Pages: 1856-1861

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Health-related QOL of Japanese men with localized prostate cancer treated with current multiple modalities assessed by a newly developed Japanense version of EPIC (the Expanded Prostate Cancer Index Composite).2007

    • Author(s)
      Kakehi Y, et al.
    • Journal Title

      Journal of Urology 177・5(印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Human agonistic antibody to tumor necrosis factor-related apotosis-inducing ligand receptor 2 (Trail-R2) induces cytotoxicity and apoptosis in prostatic cancer and bladder cancer cells.2007

    • Author(s)
      Shimada O, Kakehi Y, et al.
    • Journal Title

      Urology 69

      Pages: 395-421

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 臨床研究の新しい潮流-戦略研究の推進へ向けて 小病巣・高分子前立腺癌の治療方針に関する研究-すぐに切らなくてもよい前立腺癌を選別できるか?2007

    • Author(s)
      筧 善行
    • Journal Title

      医学の歩み 219・12

      Pages: 331-336

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Monoclonal antibody to tumor necrosis factor-related apoptosis-induciing ligand receptor2 (TRAIL-R2) induces apoptosis in primary renal cell carcinoma cells in vitro and inhibits tumor growth in vivo.2006

    • Author(s)
      Zeng Y, Kakehi Y, et al.
    • Journal Title

      International Journal of Oncology 28

      Pages: 421-430

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 新しい前立腺腫瘍マーカーEPCA.2006

    • Author(s)
      植月祐次, 筧 善行
    • Journal Title

      Biotherapy 20・4

      Pages: 427-432

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 「EBMがもたらしたもの、めざすもの」EBMは臨床現場を変えることができたか.泌尿器科領域2006

    • Author(s)
      筧 善行
    • Journal Title

      EBMジャーナル 7・1

      Pages: 154-159

    • Related Report
      2006 Annual Research Report
  • [Presentation] Autotaxin Immunohistochemical Expression Is Closely Linked to the Histopathologic Pattern in Prostate Cancer.2007

    • Author(s)
      ノウ モハメド、呉 秀賢、乾 政志、筧 善行, ほか
    • Organizer
      第95回日本泌尿器科学会総会
    • Place of Presentation
      神戸
    • Year and Date
      2007-04-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Autotaxin Immunohistochemical Expression Is Closely Linked to the Histopathologic Pattern in Prostate Cancer2007

    • Author(s)
      Nouh, Mohammed, Xiuxian, Wu, Masashi, Inui, kakehi, Y
    • Organizer
      The 95th Annual Meeting of the Japanese Urological Association
    • Year and Date
      2007-04-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Autotaxin Immunohistochemical Expression Is Closely Linked to the Histopathologic Pattern in Prostate Cancer2007

    • Author(s)
      ノウ モハメド、呉 秀賢、乾 政志、筧 善行, ほか
    • Organizer
      第95回日本泌尿器科学会総会
    • Place of Presentation
      神戸
    • Year and Date
      2007-04-14
    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi