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Identification and functional analyses of proteins which are involved in the sterility and sperm production in testis

Research Project

Project/Area Number 18390441
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionTokyo Metropolitan Organization for Medical Research

Principal Investigator

HARA Takahiko  Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 副参事研究員 (80280949)

Co-Investigator(Kenkyū-buntansha) KIYONO Mari  財団法人東京都医学研究機構, 東京都臨床医学総合研究所, 研究員 (60376621)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2008: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2007: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2006: ¥5,200,000 (Direct Cost: ¥5,200,000)
Keywords精巣 / 精子 / 精原細胞 / セルトリ細胞 / サイクリンD2 / リポカリン2
Research Abstract

精原細胞から分泌されたサイトカインは、隣接して存在するセルトリ細胞のNF-κB経路を活性化して、抗菌物質リポカリン2をコードする遺伝子の転写を高める。これが精巣を細菌感染から防御する仕組みであることを証明した。次に、精子数に影響を与える膜タンパク質S76の機能を解明するために、組織特異的な遺伝子破壊を可能とさせるS76-floxedマウス系統を新たに作出した。最後に、繊毛タンパク質CMG-1、およびRNAヘリカーゼDDX1が精原細胞の増殖分化に働く遺伝子群の転写を正に調節する、という新事実を見出した。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (29 results)

All 2009 2008 2007 2006 Other

All Journal Article (17 results) (of which Peer Reviewed: 11 results) Presentation (9 results) Remarks (3 results)

  • [Journal Article] DDX1 is required for testicular tumorigenesis, partially through the transcriptional activation of 12p stem cell genes.2009

    • Author(s)
      K. Tanaka, S. Okamoto, Y. Ishikawa, H. Tamura, and T. Hara.
    • Journal Title

      Oncogene, advance online publication April 27

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells.2009

    • Author(s)
      S. Ohata, M. Nawa, T. Kasama, T. Yamasaki, K. Sawanoboria, S. Hata, T. Nakamura, Y. Asaoka, T. Watanabe, H. Okamoto, T. Hara, S. Terai, I. Sakaida, T. Katada, and H. Nishina.
    • Journal Title

      Biochem. Biophys. Res. Commun. 379

      Pages: 817-823

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] DDX1 is required for testicular tumorigenesis, partially through the transcriptional activation of 12p stem cell genes2009

    • Author(s)
      K. Tanaka, S. Okamoto, Y. Ishikawa, H. Tamura, T. Hara
    • Journal Title

      Oncogene (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells2009

    • Author(s)
      S. Ohata, M. Nawa, 8 authors, T. Hara, S. Terai, I. Sakaida, T. Katada, H. Nishina
    • Journal Title

      Biochem. Biophys. Res. Commun. 379

      Pages: 817-823

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acceleration of chronic myeloproliferation by enforced expression of Meis1 or Meis3 in Icsbp-deficient bone marrow cells.2008

    • Author(s)
      T. Hara, M. Schwieger, R. Kazama, S. Okamoto, K. Minehata, M. Ziegler, J. Lohler, and C. Stocking.
    • Journal Title

      Oncogene 27

      Pages: 3865-3869

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acceleration of chronic myeloproliferation by enforced expression of Meis1 or Meis3 in Icsbp-deficient bone marrow cells2008

    • Author(s)
      T. Hara, M. Schwieger, R. Kazama, S. Okamoto, K. Minehata, M. Ziegler, J. Lohler, C. Stocking
    • Journal Title

      Oncogene 27

      Pages: 3865-3869

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acceleration of chronic myeloproliferation by enforced expression of Meisl or Meis3 in Icsbp-deficient bone marrow cells.2008

    • Author(s)
      T. Hara, M. Schwieger, et. al.
    • Journal Title

      Oncogene (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial bottleneck occurs without reduction of mtDNA content in mouse female germ cells.2007

    • Author(s)
      L. Cao, H. Shitara, T. Horii, Y. Nagao, H. Imai, K. Abe, T. Hara, J. Hayashi, and H. Yonekawa.
    • Journal Title

      Nat. Genet. 39

      Pages: 386-390

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial bottleneck occurs without reduction of mtDNA content in mouse female germ cells.2007

    • Author(s)
      L. Cao, H. Shitara, et. al.
    • Journal Title

      Nature Genetics 39

      Pages: 386-390

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ヒトES細胞は上胚盤葉由来幹細胞EpiSCsに相当する2007

    • Author(s)
      原 孝彦
    • Journal Title

      実験医学 25

      Pages: 2166-2167

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Mitochondrial bottleneck occurs without reduction of mtDNA content in mouse female germ cells2007

    • Author(s)
      L.Cao, H.Shitara, (4 authors), T.Hara, J.Hayashi, H.Yonekawa
    • Journal Title

      Nature Genetics 39

      Pages: 386-390

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Crucial role of the small GTPase ARF6 in hepatic cord formation during liver development.2006

    • Author(s)
      T. Suzuki, Y. Kanai, T. Hara, J. Sasaki, T. Sasaki, M. Kohara, T. Maehama, C. Taya, H. Shitara, H. Yonekawa, M. A. Frohman, T. Yokozeki, and Y. Kanaho.
    • Journal Title

      Mol. Cell Biol. 26

      Pages: 6149-6156

    • Related Report
      2008 Final Research Report
  • [Journal Article] Capillary morphogenesis gene (CMG)-1 is among the genes differentially expressed in mouse male germ line stem cells and embryonic stem cells.2006

    • Author(s)
      R. Fujino, Y. Ishikawa, K. Tanaka, M. Kanatsu-Shinohara, K. Tamura, H. Kogo, T. Shinohara and T. Hara.
    • Journal Title

      Mol. Reprod. Dev. 73

      Pages: 955-966

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spermatogonial cell-mediated activation of an IκBζ-independent NF-кB pathway in Sertoli cells induces transcription of the lipocalin-2 gene.2006

    • Author(s)
      R. Fujino, K. Tanaka, M. Morimatsu, K. Tamura, H. Kogo, and T. Hara.
    • Journal Title

      Mol. Endocrinol. 20

      Pages: 904-915

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spermatogonial cell-mediated activation of an IκBζ-independent NF-κB pathway in Sertoli cells induces transcription of the lipocalin-2 gene2006

    • Author(s)
      R.Fujino, K.Tanaka, (3 authors), T.Hara
    • Journal Title

      Molecular Endocrinology 20

      Pages: 904-915

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Capillary morphogenesis gene (CMG)-1 is among the genes differentially expressed in mouse male germ line stem cells and embryonic stem cells2006

    • Author(s)
      R.Fujino, Y.Ishikawa, (5 authors), T.Hara
    • Journal Title

      Molecular Reproduction and Development 72

      Pages: 955-966

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Crucial role of the small GTPase ARF6 in hepatic cord formation during liver development2006

    • Author(s)
      T.Suzuki, Y.Kanai, T.Hara, (9 authors), Y.Kanaho
    • Journal Title

      Molecular and Cellular Biology 26

      Pages: 6149-6156

    • Related Report
      2006 Annual Research Report
  • [Presentation] DDX1遺伝子の過剰発現が精巣腫瘍形成を促進する2008

    • Author(s)
      田中貴代子, 原孝彦
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] CMG-1は精母細胞のCyclin-D2および接着関連遺伝子の転写を制御する2008

    • Author(s)
      大林謙一郎, 田中貴代子, 藤野隆介, 田村和広, 原孝彦
    • Organizer
      第6回幹細胞シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 精巣腫瘍形成におけるDDX1の役割2008

    • Author(s)
      田中貴代子, 原孝彦
    • Organizer
      第6回幹細胞シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] Ddx1遺伝子の精巣形成過程における発現と機能解析2007

    • Author(s)
      大林謙一郎、田中貴代子、藤野隆介、田村和広、向後博司、原孝彦
    • Organizer
      第30回日本分子生物学会年会BMB2007
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Pre-leukemic amplification of granulocytes by enforced expression of Meis1 or Meis3 in hematopoietic stem cells of ICSBP-deficient mice.2007

    • Author(s)
      T. Hara, M. Schwieger, R. Kazama, S. Okamoto, and C. Stocking.
    • Organizer
      5th Annual Meeting of International Society for Stem Cell Research (ISSCR)
    • Place of Presentation
      Cairns, Australia
    • Related Report
      2008 Final Research Report
  • [Presentation] Meis1/3遺伝子はICSBP欠損マウスの顆粒球分化を促進する2007

    • Author(s)
      原孝彦、Maike Schwieger、風間律子、岡本士毅、Carol Stocking
    • Organizer
      第5回幹細胞シンポジウム
    • Place of Presentation
      淡路島
    • Related Report
      2008 Final Research Report
  • [Presentation] Ddx1遺伝子の精巣形成過程における発現と機能解析2007

    • Author(s)
      田中 貴代子、中山 由紀、原 孝彦
    • Organizer
      第30回日本分子生物学会年会-BMB2007
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Physiological function of the small G protein ARF6 in hepatic cord formation and liver development.2006

    • Author(s)
      T. Suzuki, Y. Kanai,Y, T. Hara, J. Sasaki, T. Sasaki, M. Kohara, T. Maehama, T. Yokozeki, and Y. Kanaho.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-06-18
    • Related Report
      2008 Final Research Report
  • [Presentation] A spermatogonial stem cell-derived factor induces the transcription of lipocalin-2/24p3 gene in Sertoli cells by an IкBκζ-independent NF-κB pathway.2006

    • Author(s)
      T. Hara, R. Fujino, K. Tanaka, M. Morimatsu, K. Tamura, and H. Kogo.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.rinshoken.or.jp/org/TB/index-jp.htm

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.rinshoken.or.jp/org/TB/index-jp.htm

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.rinshoken.or.jp/org/TB/index-jp.htm

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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