| Project/Area Number |
18390444
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Akita University |
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Principal Investigator |
TANAKA Toshinobu Akita University, School of Medicine, Professor (40002216)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Jun Akita University, School of Medicine, Associate professor (80250877)
KAWAMURA Kazuhiro Akita University, School of Medicine, Associate professor (10344756)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥14,220,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥720,000)
Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2006: ¥11,100,000 (Direct Cost: ¥11,100,000)
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| Keywords | oocyte maturation / DNA microarray / paracrine factor / granulosa cell / IVF-ET |
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| Research Abstract |
Using the DNA microarray analyses, we found some candidates for novel oocyte maturation factors. Final candidates were chosen based on the localization of the ligands in ovarian somatic cells and their cognate receptors in oocytes and/or cumulus cells. We could get approximately 20 candidates in the assay. Then, we have performed functional analyses for oocyte nuclear maturation to the final candidates. Immature oocytes were treated with the ligands to examine their roles in germinal vesicle breakdown (GVBD) and extrusion of first polar body. We found factors could induce GVBD, extrusion of first polar body, or both GVBD and extrusion of first polar body. Furthermore, we examined their contribution to cytoplasmic maturation and found several factors could induce cytoplasmic maturation. Among novel identified factors, we reported grail-cell line derived neurotrophic factor. Some reports for other factors are submitted to journals and are under review. The novel oocyte maturation factors
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identified based on animal studies were further explored in human oocyte maturation by examining their expression and effects on oocyte maturation. Using ovaries and surplus unfertilized oocytes obtained from patients underwent surgical treatment and IVF-ET, respectively, the expression of the ligands and their receptors was determined by RT-PCR and immunohistochemistry. We found the novel oocyte maturation factors in human cumulus and granulosa cells or theca cells and their receptors in oocytes. Furthermore, we examined the association of oocyte maturation with the levels of novel oocyte maturation factors in human follicular fluid and cumulus cells obtained from patients underwent IVF-ET. Although we could find positive tendencies in the association, they were not reached significant levels due to small number of samples. Thus, we try to get more samples for this study. Because we have no patients who could receive full informed consent, studies on genetical abnormality in patients have not performed. We expect to find such patient in future. We believe that our findings lead identification of novel oocyte maturation factors and their regulations in human, and shed a light on the clinical application for establishment of in vitro maturation of oocytes and development of novel methods for obtaining good-quality mature oocytes in IVF-ET treatment. Less
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