• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on in-vitro culture system for oocyte maturation and embryo growth originated from cryopreserved ovaries

Research Project

Project/Area Number 18390453
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionHyogo College of Medicine

Principal Investigator

KAYAMA Koji  Hyogo College of Medicine, 医学部, 教授 (00068496)

Co-Investigator(Kenkyū-buntansha) AKATANI Akiko  兵庫医科大学, 医学部, 准教授 (50212402)
OGASAWARA Toshitada  兵庫医科大学, 医学部, 講師 (30213995)
KASUMI Hiroyuki  兵庫医科大学, 医学部, 講師 (00289068)
KUMAMOTO Kanako  兵庫医科大学, 医学部, ポストドクター (10469322)
Research Collaborator MOCHIDA Nahoko  
GIANNINA Calongos  
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥16,710,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2006: ¥8,000,000 (Direct Cost: ¥8,000,000)
Keywords卵巣バンク / 生殖補助医療 / ガラス化保存法 / 不妊症 / 卵巣障害 / 卵母細胞 / 卵胞発育 / IVG / IVM
Research Abstract

ガラス化法を応用して、卵巣組織の低温保存法の至適条件を検討し、複数の動物種に有効な条件を確立した。低温保存後、組織を融解し、前胞状期卵胞を培養により発育・成熟させ、体外受精により新鮮組織と遜色のない良好胚を得ることに成功した。偽妊娠マウスに胚を移植することにより、健康な産仔の獲得にも成功した。さらに発育段階の早い卵胞についても効率的な培養法を開発した。この結果は、卵巣の生殖資源としての利用ならびに難治性不妊症の治療法の開発に応用可能である。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (63 results)

All 2009 2008 2007 2006

All Journal Article (20 results) (of which Peer Reviewed: 15 results) Presentation (37 results) Book (6 results)

  • [Journal Article] Harmful effects of anti-zona pellucida antibodies in folliculogenesis, oogenesis and fertilization.2009

    • Author(s)
      Calongos, G., Hasegawa, A., Komori, S., Koyama, K.
    • Journal Title

      J. Reprod. Immunol. 79

      Pages: 148-155

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional Aspects of CD52 in Reproduction2009

    • Author(s)
      Koyama K, Hasegawa A
    • Journal Title

      J Reprod Immunol. (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Harmful effects of anti-zona pellucida antibodies in folliculogenesis, oogenesis, and fertilization.2009

    • Author(s)
      Calongos G, Hasegawa A, Komori S, Koyama K.
    • Journal Title

      J Reprod Immunol. 79

      Pages: 148-55

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of urinary and recombinant follicle- stimulating hormone in in vitro growth, maturation, and fertilization of mouse preantral follicles.2008

    • Author(s)
      Calongos, G., Hasegawa, A., Komori, S., Koyama, K.
    • Journal Title

      Fertil. Steril. 89

      Pages: 1482-1489

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 卵子の体外発育と体外成熟2008

    • Author(s)
      赤谷(長谷川)昭子, 香山浩二
    • Journal Title

      産婦人科治療 9

      Pages: 12-17

    • Related Report
      2008 Final Research Report
  • [Journal Article] 卵巣組織の凍結保存と生殖補助医療への応用2008

    • Author(s)
      赤谷(長谷川)昭子, 香山浩二
    • Journal Title

      兵庫医大医学会雑誌 33

      Pages: 55-60

    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Journal Article] Comparison of urinary and recombinant follicle-stimulating hormone in in-vitro growth, maturation, and fertilization of mouse preantral follicles.2008

    • Author(s)
      Calongos G, Hasegawa A, Komori S, Koyama K.
    • Journal Title

      Fertil Steril. 89

      Pages: 1482-9

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CD52 is synthesized in cumulus cells and secreted into the cumulus matrix during ovulation.2008

    • Author(s)
      Hasegawa A, Takenobu, T, Kasumi, H, Komori, S, Koyama K
    • Journal Title

      Am. J. Reprod. Immunol. 60

      Pages: 187-91

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of zona pellucida proteins to ovarian folliculogenesis. Reprod.2007

    • Author(s)
      Hasegawa, A., Kanazawa, N., Koyama, K.
    • Journal Title

      Immunol. Biol. 221

      Pages: 1-7

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ovarian Tissue Banking.2007

    • Author(s)
      Hasegawa, A., Mochida, N. Kasumi H., Komori, S, Koyama, K.
    • Journal Title

      J.Mamm. Ova Res. 24

      Pages: 8-13

    • NAID

      10019488037

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro growth and maturation of mouse oocyte-granulosa cell complex from cryopreserved ovaries and achievement of pup birth.2007

    • Author(s)
      Hasegawa, A., Koyama, K.
    • Journal Title

      Repro. Med. Biol. 6

      Pages: 77-83

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 卵巣の凍結保存2007

    • Author(s)
      香山浩二
    • Journal Title

      医学の歩み 223

      Pages: 115-121

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of zona proteins to oocyte growth2007

    • Author(s)
      Hasegawa, A., Koyama, K.
    • Journal Title

      Society of Reproduction Fertility(Suppl.) 63

      Pages: 229-235

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro growth and maturation of mouse oocyte-granulosa cell complex from cryopreserved ovaries and achievement of pup birth2007

    • Author(s)
      Hasegawa, A., Koyama, K.
    • Journal Title

      Reproductive Medicine and Biology 6

      Pages: 77-83

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ovarian Tissue Banking2007

    • Author(s)
      Hasegawa, A., Koyama, K.
    • Journal Title

      Journal of Ova Research 24

      Pages: 8-13

    • NAID

      10019488037

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 卵子の体外発育と体外成熟2007

    • Author(s)
      長谷川 昭子, 香山 浩二
    • Journal Title

      産産婦人科治療 96

      Pages: 12-17

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 卵子の体外発育と体外成熟2007

    • Author(s)
      香山 浩二
    • Journal Title

      医学のあゆみ 223

      Pages: 115-121

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Pup birth from mouse oocytes in preantral follicles derived from vitrified and warmed ovaries followed by in vitro growth, in vitro maturation and in vitro fertilization.2006

    • Author(s)
      Hasegawa, A., Sawai, H., Mochida, N, Ogasawara, T., Koyama, K.
    • Journal Title

      Fertil. Steril. 86

      Pages: 1182-1192

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Premature ovarian failure syndrome may be induced by autoimmune reaction to zona pellucida proteins.2006

    • Author(s)
      Koyama, K., Hasegawa, A.
    • Journal Title

      Reproductive Medicine and Endocrinology 3

      Pages: 94-97

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pub birth from mouse oocytes in preantral follicles derived from vitrified and warmed ovaries followed by in vitro growth, in vitro maturation, and in vitro fertilization2006

    • Author(s)
      Hasegawa A., Mochida N., Ogasawara T., Koyama K.
    • Journal Title

      Fertility and Sterility 86・3

      Pages: 1182-1192

    • Related Report
      2006 Annual Research Report
  • [Presentation] 初期卵胞発育培養法に関する検討2009

    • Author(s)
      赤谷(長谷川)昭子
    • Organizer
      (シンポジウム)第53回日本生殖医学会総会・学術講演会
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] Molecular mechanisms of ovarian folliculogenesis2009

    • Author(s)
      Hasegawa, A. Koyama, K
    • Organizer
      International Congress on Bio-immunoregulatory Mechanisms Associated with Reproductive Organs
    • Place of Presentation
      New Delhi (India)
    • Related Report
      2008 Annual Research Report
  • [Presentation] Role of zona pellucida in folliculogenesis and post-fertilization events2009

    • Author(s)
      Hasegawa, A. Koyama, K
    • Organizer
      VI European Congress of Reproductive Immunology
    • Place of Presentation
      Moscow (Russia)
    • Related Report
      2008 Annual Research Report
  • [Presentation] 2段階IVG(In VitroGrowth)によるマウス初期卵胞の発育誘導2008

    • Author(s)
      持田菜穂子, 赤谷(長谷川)昭子, 熊本香名子, 小森慎二, 香山浩二
    • Organizer
      第23回日本生殖免疫学会総会・学術集会
    • Place of Presentation
      富山
    • Related Report
      2008 Final Research Report
  • [Presentation] マウス初期発育卵胞の2段階IVG(In Vitro Growth)による発育誘導と卵母細胞の成熟2008

    • Author(s)
      持田菜穂子, 赤谷(長谷川)昭子, 小森慎二, 香山浩二
    • Organizer
      第53回日本生殖医学会総会・学術講演会
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] Development of early growing mouse follicles by the two-step culture method.2008

    • Author(s)
      Wada, R., Hasegawa, A., Mochida, N., Komori, S. and Koyama, K.
    • Organizer
      6th European Congress of Reproductive Immunology
    • Place of Presentation
      Moscow, Russian Federation
    • Related Report
      2008 Final Research Report
  • [Presentation] A two-step culture system for development of mouse early preantral follicles.2008

    • Author(s)
      Kinuta, T., Komori, S., Hasegawa A., Mochida, N. and Koyama, K.
    • Organizer
      41st Annual Meeting of the Society for the Study of Reproduction (SSR 2008)
    • Place of Presentation
      Hawaii, USA.
    • Related Report
      2008 Final Research Report
  • [Presentation] 透明帯の卵胞発育および受精における役割-透明帯欠損マウスを用いた検討-2008

    • Author(s)
      赤谷(長谷川)昭子, 楊 信志, 小森慎二, 香山浩二
    • Organizer
      第49回日本哺乳動物卵子学会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] マウス初期発育卵胞の2段階IVG(In vitro growth)による発育誘導と卵母細胞の成熟2008

    • Author(s)
      持田菜穂子, 赤谷(長谷川)昭子, 小森慎二, 香山浩二
    • Organizer
      第49回日本哺乳動物卵子学会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] Development of early growing mouse follicles by the two-step culture method2008

    • Author(s)
      Wada, R. Hasegawa, A., et al.
    • Organizer
      VI European Congress of Reproductive Immunology
    • Place of Presentation
      Moscow (Russia)
    • Related Report
      2008 Annual Research Report
  • [Presentation] 2-3層の顆粒膜細胞で覆われた初期二次卵胞卵母細胞の発育及び成熟培養2008

    • Author(s)
      長谷川昭子, 霞弘之, 他
    • Organizer
      第60回日本産科婦人科学会学術講演会
    • Place of Presentation
      横浜
    • Related Report
      2008 Annual Research Report
  • [Presentation] 抗透明帯抗体の卵胞発育におよぼす影響とその障害メカニズムについて2008

    • Author(s)
      Calongos Giannina, 長谷川昭子, 他
    • Organizer
      第60回日本産科婦人科学会学術講演会
    • Place of Presentation
      横浜
    • Related Report
      2008 Annual Research Report
  • [Presentation] マウス初期発育卵胞の2段階IVG(In vitro growth)による発育誘導と卵母細胞の成熟2008

    • Author(s)
      持田菜穂子, 長谷川昭子, 他
    • Organizer
      第49回哺乳動物卵子学会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] 透明帯の卵胞発育および受精における役割 : 透明帯欠損マウスを用いた検討2008

    • Author(s)
      長谷川昭子, 楊信志, 他
    • Organizer
      第49回哺乳動物卵子学会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] 初期卵胞発育培養法に関する検討2008

    • Author(s)
      長谷川昭子(シンポジウム)
    • Organizer
      第53回日本生殖医学会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] マウス初期発育卵胞の2段階IVGによる発育誘導と卵母細胞の成熟2008

    • Author(s)
      持田菜穂子, 長谷川昭子, 他
    • Organizer
      第53回日本生殖医学会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] 2段階IVGによるマウス初期卵胞の発育誘導2008

    • Author(s)
      持田菜穂子, 長谷川昭子, 他
    • Organizer
      第23回日本生殖免疫学会
    • Place of Presentation
      富山
    • Related Report
      2008 Annual Research Report
  • [Presentation] マウス初期発育卵胞の2段階IVGによる発育と成熟および卵子受精能2007

    • Author(s)
      持田菜 穂子, 長谷川 昭子, 他
    • Organizer
      第48回哺乳動物卵子学会
    • Place of Presentation
      甲府
    • Year and Date
      2007-05-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] マウス凍結保存卵巣由来未発育卵母細胞の培養による発育誘導と産仔への発生について2007

    • Author(s)
      赤谷(長谷川)昭子, 霞弘之, 持田菜穂子, 小森慎二, 香山浩二
    • Organizer
      第132回日本生殖医学会関西支部集談会・第34回関西アンドロロジーカンファレンス
    • Place of Presentation
      大阪
    • Year and Date
      2007-03-03
    • Related Report
      2008 Final Research Report
  • [Presentation] Preantral follicleの体外発育と成熟 : 動物実験を中心に2007

    • Author(s)
      赤谷(長谷川) 昭子, 香山浩二
    • Organizer
      第2回日本生殖医療エンジニアリング研究会学術集会,シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2007-02-25
    • Related Report
      2008 Final Research Report
  • [Presentation] 卵巣組織の凍結保存と生殖医療への応用2007

    • Author(s)
      赤谷(長谷川)昭子, 香山浩二
    • Organizer
      第7回RMB(生殖医学・生物学)研究会シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2007-01-13
    • Related Report
      2008 Final Research Report
  • [Presentation] Possible role of zona pellucida proteins in ovarian follicle development.2007

    • Author(s)
      Hasegawa, A., Mochida, N., Kasumi, H., Komori, S., Koyama, K.
    • Organizer
      (Invited) X international congress of reproductive immunology
    • Place of Presentation
      Croatia
    • Related Report
      2008 Final Research Report
  • [Presentation] Early follicle development using a two-step culture system.2007

    • Author(s)
      Hasegawa, A., Mochida, N., Komori, S. and Koyama, K.
    • Organizer
      The international Ovarian Conference 2007
    • Place of Presentation
      Hakone
    • Related Report
      2008 Final Research Report
  • [Presentation] Comparison of urinary and recombinant FSH in-vitro growth, maturation and fertilization of mouse preantral follicles.2007

    • Author(s)
      Calongos, G., Hasegawa, A. Komori, S. and Koyama, K.
    • Organizer
      The International Ovarian Conference 2007
    • Place of Presentation
      Hakone
    • Related Report
      2008 Final Research Report
  • [Presentation] マウス初期発育卵胞の2段階IVF ( In Vitro Growth )による発育と成熟2007

    • Author(s)
      持田菜穂子, 赤谷(長谷川)昭子, 霞 弘之, 小森慎二, 香山浩二
    • Organizer
      第25回日本受精着床学会総会・学術講演会
    • Place of Presentation
      仙台
    • Related Report
      2008 Final Research Report
  • [Presentation] Live birth from parenteral follicle oocytes in cryopreserved mouse ovaries after IVG, IVM and IVF2007

    • Author(s)
      Horiuchi, I., Hasegawa, A., Kasumi, H., Mochida, N., Komori, S. and Koyama, K.
    • Organizer
      X International Congress of Reproductive Immunology
    • Place of Presentation
      Opatija, Croatia
    • Related Report
      2008 Final Research Report
  • [Presentation] マウス初期発育卵胞の2段階IVG(In vitro growth)による発育と成熟および卵子受精能2007

    • Author(s)
      持田菜穂子, 赤谷(長谷川)昭子, 霞弘之, 小森慎二, 香山浩二
    • Organizer
      第48回日本哺乳動物卵子学会
    • Place of Presentation
      甲府
    • Related Report
      2008 Final Research Report
  • [Presentation] 透明帯タンパクの卵胞および卵母細胞発育における役割2007

    • Author(s)
      赤谷(長谷川)昭子, 持田菜穂子, 霞 弘之, 小森慎二, 香山浩二
    • Organizer
      第48回日本哺乳動物卵子学会
    • Place of Presentation
      甲府
    • Related Report
      2008 Final Research Report
  • [Presentation] Possible role zona pellucida proteins in ovarian follicle development2007

    • Author(s)
      Hasegawa, A., Koyama, K
    • Organizer
      X International Congress of Reproductive Immunology
    • Place of Presentation
      Opatiya(Croatia)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Presence and function of CD52 in female reprodutive tracts2007

    • Author(s)
      Hasegawa, A., Koyama, K
    • Organizer
      27th Annual Meeting of America Society for Repro ductive Immunology
    • Place of Presentation
      Toronto(Canada)
    • Related Report
      2007 Annual Research Report
  • [Presentation] 顆粒膜細胞におけるCD52の発現2007

    • Author(s)
      武信 尚史, 長谷川 昭子, 他
    • Organizer
      第59回日本産科婦人科学会学術講演会
    • Place of Presentation
      京都
    • Related Report
      2007 Annual Research Report
  • [Presentation] マウスIn vitro 卵胞発育におけるUrinary FSHとrecombinant FSHの比較2007

    • Author(s)
      Calongos Giannina, 長谷川 昭子, 他
    • Organizer
      第59回日本産科婦人科学会学術講演会
    • Place of Presentation
      京都
    • Related Report
      2007 Annual Research Report
  • [Presentation] Early follicle development using a two-step culture system2007

    • Author(s)
      Hasegawa, A., Mochida, N., et. al.
    • Organizer
      The International Conference 2007
    • Place of Presentation
      箱根
    • Related Report
      2007 Annual Research Report
  • [Presentation] Comparison of urinary and recombinant FSH in in-vitro growth,maturation and fertilization of mouse preantral folliciles2007

    • Author(s)
      Calongos, G, Hasegawa, A., et. al.
    • Organizer
      The International Conference 2007
    • Place of Presentation
      箱根
    • Related Report
      2007 Annual Research Report
  • [Presentation] 卵丘細胞におけるCD52の性状分析2007

    • Author(s)
      武信 尚史, 長谷川 昭子, 他
    • Organizer
      第回日本生殖免疫学会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] 卵巣組織の凍結保存2006

    • Author(s)
      赤谷(長谷川)昭子, 持田菜穂子, 香山浩二
    • Organizer
      (シンポジウム)第24回日本ヒト細胞学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 生殖補助医療に用いられる培養液の検討2006

    • Author(s)
      赤谷(長谷川)昭子, 持田菜穂子, ジャニーナ・カロンゴス, 香山浩二
    • Organizer
      (シンポジウム)第47回日本哺乳動物卵子学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Book] The role of male reproductive tract CD52 (mrt-CD52) in reproduction. "Gamete Biology : Emerging Frontiers in Fertility and Contraceptive Development" (ed. by Gupta SK, Koyama K, Murray JF)2007

    • Author(s)
      Koyama, K., Ito K., Hasegawa, A.
    • Publisher
      Soc Reprod Fertil Suppl.
    • Related Report
      2008 Final Research Report
  • [Book] Contribution of zona proteins to oocyte growth. "Gamete Biology : Emerging Frontiers in Fertility and Contraceptive Development" (ed. by Gupta SK, Koyama K, Murray JF),2007

    • Author(s)
      Hasegawa, A., Koyama, K.
    • Publisher
      Soc Reprod Fertil Suppl.
    • Related Report
      2008 Final Research Report
  • [Book] 受精, 「図説よくわかる臨床不妊学入門」, (柴原浩章・森本義晴・京野廣一 : 監修)2007

    • Author(s)
      赤谷(長谷川)昭子, 香山浩二
    • Publisher
      中外出版
    • Related Report
      2008 Final Research Report
  • [Book] コメディカルのための臨床不妊学入門2007

    • Author(s)
      長谷川 昭子, 香山 浩二(共著)
    • Total Pages
      316
    • Publisher
      中外出版
    • Related Report
      2007 Annual Research Report
  • [Book] 体外受精への移行タイミング, 「図説よくわかる臨床不妊学入門」, (柴原浩章・森本義晴・京野廣一 : 監修)2006

    • Author(s)
      香山浩二
    • Publisher
      中外出版
    • Related Report
      2008 Final Research Report
  • [Book] 精子免疫, 新編「精子学」, (毛利秀雄・星元紀 監修)2006

    • Author(s)
      香山浩二
    • Publisher
      東京大学出版
    • Related Report
      2008 Final Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi