Direct functional analysis of prestin transfected inner ear using invivo patch method

• Project/Area Number: 18390454
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Hirosaki University
• Principal Investigator: KAKEHATA Seiji Hirosaki University, 大学院・医学研究科, 准教授 (90261619)
• Co-Investigator(Kenkyū-buntansha): UENO Shinya 弘前大学, 大学院・医学研究科, 教授 (00312158) ; MARUYA Shin-ichiro 国際医療福祉大学, 大学病院, 講師 (90396408) ; NAMBA Atsushi 弘前大学, 医学部附属病院, 助教 (50361027) ; KITANI Rei 弘前大学, 医学部附属病院, 医員 (80436047)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,570,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥2,370,000); Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2007: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2006: ¥6,300,000 (Direct Cost: ¥6,300,000)
• Keywords: 内耳再生 / コルチ器 / 外有毛細胞 / prestin / 運動能 / パッチクランプ法 / キャパシタンス / ソノポレーション法

Research Abstract

C57BL/6Jマウス内耳外有毛細胞(OHC)におけるprestin 発現様式は、発達段階早期にOHC 細胞膜に発現してからmaturation とともに機能を獲得するという、これまでラットなどで知られていたものとは全く異なる、新しい様式であることがわかった。また、正常聴力獲得のために必要なprestin 電荷密度は9,400 e-/□m2 以上であることが示唆された。
Gjb2 優性ネガティブ変異遺伝子改変マウスにおいても同様の発現様式であることが確認された。

Research Products

• [Journal Article] (corresponding author) Developmental expression of the outer hair cell motor, prestin, in the mouse (2007)
  • Author(s): Abe T, Kakehata S, Kitani R, Maruya SI, Navaratnam D, Santos-Sacchi J, Shinkawa H
  • Journal Title: J Membr Biol 215 Pages: 49-56
  • Peer Reviewed
• [Journal Article] Developmental expression of the outer hair cell motor, prest in, in the mouse. (2007)
  • Author(s): Abe T, Kakehata S, et.al(corresponding author)
  • Journal Title: Journal of Membrane Biology 215 Pages: 49-56
  • Peer Reviewed
• [Journal Article] Tuning in to the amazing outer hair cell:membrane wizardry with a twist and shout. (2007)
  • Author(s): He D, Zhen J, Kalinec F, Kakehata S. Santos-Sacchi J.
  • Journal Title: Journal of Membrane Biology 209 Pages: 119-134
• [Journal Article] Tuning in to the amazingouter hair cell : membrane wizardry with atwist and shout (2006)
  • Author(s): He DZ, Zheng J, Kalinec F, Kakehata S, Santos-Sacchi J
  • Journal Title: J Membr Biol 209(2-3) Pages: 119-34
  • Peer Reviewed
• [Journal Article] CochlearOuter Hair Cells in a Dominant-NegativeConnexin26 Mutant Mouse Preserved Non-Linear Capacitance in spite ofImpaired Distortion Product OtoacousticEmission
  • Author(s): Minekawa A, Abe T, Inoshita A, Iizuka T, Kakehata S, Narui Y, Koike T, Kamiya K, Okamura H, Shinkawa H, Ikeda K
  • Journal Title: Neuroscience (in press)
  • Peer Reviewed
• [Presentation] DevelopmentalExpression of the Outer Hair CellCapacitance in a Dominant-NegativeConnexin26 Mutant Mouse. 32nd MidWinterMeeting of Association for (2009)
  • Author(s): Abe T, Kakehata S, et.al.
  • Organizer: Research in Otolaryngology
  • Place of Presentation: Baltimore, MD, USA
• [Presentation] CochlearOuter Hair Cells in a Dominant-Negative Connexin26 Mutant Mouse PreservedNon-Linear Capacitance in spite ofImpaired Distortion Product OtoacousticEmission. (2009)
  • Author(s): Minekawa A, Abe T, Inoshita A, Iizuka T,Kakehata S, Narui Y, Koike T, Kamiya K, Okamura H, Shinkawa H, Ikeda K
  • Organizer: 32nd MidWinter Meeting ofAssociation for Research in Otolaryngology
  • Place of Presentation: Baltimore, MD, USA
• [Presentation] Developmental Expression of the Outer Hair Cell Capacit ance in a Dominant-Negative Connexin26 Mutant Mouse (2009)
  • Author(s): Abe T, Kakehata S, et.al.
  • Organizer: 32nd MidWinter Meeting of Association for Research in Otolaryngology
  • Place of Presentation: Baltimore, MD, USA
• [Presentation] Cochlear Outer Hair Cells in a Dominant-Negative Connexin26 Mutant Mouse Preserved Non-Linear Capacitance with Impaired Distortion Product Otoacoustic Emission (2009)
  • Author(s): Abe T, Kakehata S, et.al.
  • Organizer: 32nd MidWinter Meeting of Association for Research in Otolaryngology
  • Place of Presentation: Baltimore, MD, USA
• [Presentation] Gjb2変異マウスにおける蝸牛外有毛細胞の運動能の解析 (2008)
  • Author(s): 阿部尚央、欠畑誠治, 他
  • Organizer: 第18回日本耳科学会総会学術講演会
  • Place of Presentation: 神戸
  • Year and Date: 2008-10-16
• [Presentation] 幼若期のGjb2変異マウスの歪耳音響放射の測定および外有毛細胞の機能評価について (2008)
  • Author(s): 峯川、阿部尚央、欠畑誠治, 他
  • Organizer: 第18回日本耳科学会総会学術講演会
  • Place of Presentation: 神戸
  • Year and Date: 2008-10-16
• [Presentation] DirectEffects of Cholesterol and Mβcd On OuterHair Cell Motility and Capacitance (2008)
  • Author(s): Kitani R, Kakehata S, Murakoshi M, WadaH, Maruya S, Abe T, Shinkawa H
  • Organizer: 31st MidWinter Meeting of Association for Research in Otolaryngology
  • Place of Presentation: Phoenix, Arizona, USA
• [Presentation] The direct effects ofcholesterol on outer hair cell motility (2006)
  • Author(s): Kitani R, Kakehata S, Murakoshi M, WadaH, Shinkawa H
  • Organizer: The 2nd Shanghai International Conference on Physiological Biophysics-Audition andVision
  • Place of Presentation: Shanghai, China
• [Presentation] The effects ofcholesterol on outer hair cell motility (2006)
  • Author(s): Kitani R, Kakehata S, Murakoshi M, Wada H, Abe T, Shinkawa H
  • Organizer: INNER EAR BIOLOGY Workshop
  • Place of Presentation: Montpellier, France