Systematical investigations on the laryngeal functions and disorders

• Project/Area Number: 18390461
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: HISA Yasuo Kyoto Prefectural University of Medicine, 医学研究科, 教授 (50181087)
• Co-Investigator(Kenkyū-buntansha): BAMBA Hitoshi 京都府立医科大学, 医学研究科, 講師 (30360035) ; SHIMADA Taketoshi 京都府立医科大学, 医学研究科, 助教 (30275226)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥15,590,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥1,590,000); Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2007: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2006: ¥8,700,000 (Direct Cost: ¥8,700,000)
• Keywords: 喉頭 / 疑核運動神経細胞異所性局在 / 神経栄養因子 / 時計遺伝子 / Fibroblast growth factor-1 / TAR DNA binding protein 43 / アセチルコリン基転移酵兼 / 腺分泌 / 筋萎縮性側索硬化症(ALS) / TAR DNA binding protein43(TDP43) / 迷走神経背側核 / アセチルコリン基転移酵素 / 選択的スプライシング / 喉頭腺分泌 / 視交叉上核 / Fibroblast growth factor-l / Shaking Rat Kawasaki / 疑核運動神経細胞 / 神経調節 / 運動神経細胞異所性局在

Research Abstract

喉頭神経調節の仕組みとその障害における現状での重要課題として考えられた、延髄における疑核異所性局在、喉頭神経系におけるアセチルコリン合成酵素の関与、喉頭神経系における神経栄養因子の関与、脳幹運動神経細胞における軸索損傷時のTDP43の変化、そして、喉頭における概日リズムと時計遺伝子の関与について、現時点で考えうる手法を駆使して解明した。

Research Products

• [Journal Article] Axotomy alters alternative splicing Of choline acetyltransferase in the rat dorsal motor nucleus of the vagus nerve (2009)
  • Author(s): Saito A, Sato T, Okano H, Toyoda K, Barnba H, Kimura S, Bellier J-P, Matsuo A, Kimura H, Hisa Y, Tooyama I
  • Journal Title: J Comp Neur 513(2) Pages: 237-248
  • Peer Reviewed
• [Journal Article] Axotomy alters alternative splicing of choline acetyltransferase in the rat dorsal motor nucleus of the vagus nerve. (2009)
  • Author(s): Saito A, Sato T, Okano H, Toyoda K, Bamba H, Kimura S, Bellier J-P, Matsuo A, Kimura H, Hisa Y
  • Journal Title: J Comp Neurol 513 Pages: 237-248
  • Peer Reviewed
• [Journal Article] Circadian gene expression in the murine larynx (2008)
  • Author(s): Nishio T, Bando H, Bamba H, Hisa Y, Okamura H
  • Journal Title: Auris Nasus Lar 35(4) Pages: 539-544
  • Peer Reviewed
• [Journal Article] 気道時計遺伝子発現と迷走神経による制御 (2008)
  • Author(s): 久育男, 板東秀樹, 西尾健志, 馬場均
  • Journal Title: 耳展 51(5) Pages: 278-285
  • NAID: 130004546238
  • Peer Reviewed
• [Journal Article] Circadian gene expression in the murine larynx. (2008)
  • Author(s): Nishio T, Bando H, Bamba H, Hisa Y, Okamura H.
  • Journal Title: Auris Nasus Larynx 35 Pages: 539-544
  • Peer Reviewed
• [Journal Article] 気道時計遺伝子発現と迷走神経による制御 (2008)
  • Author(s): 久 育男, 板東秀樹, 西尾健志, 馬場均
  • Journal Title: 耳展 51 Pages: 278-285
  • NAID: 130004546238
  • Peer Reviewed
• [Journal Article] Low expression of FGFl(fibroblast growth factor-1)in rat parasympathetic preganglionic neurons (2007)
  • Author(s): Saito A, Okano H, Bamba H, Hisa Y, Oomura Y, Imamura T, Tooyama I
  • Journal Title: Histol Histopathol 22(12) Pages: 1327-1335
  • Peer Reviewed
• [Journal Article] Vagal regulation of respiratory clocks in mice (2007)
  • Author(s): Bando H, Nishio T, van der Horst GT, Masubuchi S, Hisa Y, Okamura H
  • Journal Title: J Neurosci 27(16) Pages: 4359-4365
  • Peer Reviewed
• [Journal Article] Ambiguus motoneurons innervating laryngeal and esophageal muscles are malpositioned in the Reelin-deficient mutant rat, shaking Rat Kawasaki (2007)
  • Author(s): Hirasugl K, Hisa Y, Setsu T, Terashima T
  • Journal Title: Acta Otolaryngol 127(2) Pages: 213-220
  • Peer Reviewed
• [Journal Article] Low expression of FGF1(fibroblast growth factor-1)in rat parasympathetic preganglionic neurons. (2007)
  • Author(s): Saito A, Okano H, Bamba H, Hisa Y, Oomura Y, Imamura T, Tooyama I.
  • Journal Title: Histol Histopathol 22 Pages: 1327-1335
  • Peer Reviewed
• [Journal Article] Vagal regulation of respiratory clocks in mice. (2007)
  • Author(s): Bando H, Nishio T, van der Horst GT, Masubuchi S, Hisa Y, Okamura H.
  • Journal Title: J Neurosci 27 Pages: 4359-4365
  • Peer Reviewed
• [Journal Article] Ambiguus motoneurons innervating laryngeal and esophageal muscles are malpositioned in the Reelin-deficient mutant rat, Shaking Rat Kawasaki (2007)
  • Author(s): Hirasugi K, Hisa Y, Setsu T, Terashima T.
  • Journal Title: Acta Otolaryngol 127 Pages: 213-220
  • Peer Reviewed
• [Journal Article] Vagal regulation of respiratory clocks in mice. (2007)
  • Author(s): Bando H, Nishio T, van der Horst GT, Masubuchi S, Hisa Y, Okamura H
  • Journal Title: J Neurosci. 27・16 Pages: 4359-65
• [Journal Article] Ambiguous motoneurons innervating laryngeal and esophageal muscles are malpositioned in the Reelin-deficient mutant rat, Shaking Rat Kawasaki. (2007)
  • Author(s): Hirasugi K, Hisa Y, Setsu T, Terashima T.
  • Journal Title: Acta Otolaryngol 127・2 Pages: 213-20
• [Presentation] Axonal strangulation induced peripheral accumulation and nuclear reduction of TDP43 in bralnstem motor neurons (2008)
  • Author(s): Satoh T,Takeuchi S,Saitoh A,Hisa Y, Tooyama I, Urushitani M
  • Organizer: Neuroscience 2008(The 38th Annual Meeting of the Soclety for Neuroscience)
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2008-11-15
• [Presentation] Axonal strangulation induced peripheral accumulation and nuclear reduction of TDP43 in brainstem motor neurons. (2008)
  • Author(s): Satoh T, Takeuchi S, Saitoh A, Hisa Y, Tooyama I, Urushitani M.
  • Organizer: Neuroscience 2008 (The 38th Annual Meeting of the Society for Neuroscience)
  • Place of Presentation: Washington DC, U.S.A.
  • Year and Date: 2008-11-15
• [Presentation] Vagal regulation of respiratory clocks in mice (2008)
  • Author(s): Bando H, Nishio T, Hirota R, Bamba H, Okamura H, Hisa Y
  • Organizer: 15th World Congress for Bronchoesopagology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-04-01
• [Presentation] Symposlum 4, Recent advances ln head and neck surgery(chairperson) (2007)
  • Author(s): Hisa Y
  • Organizer: The lnternational Colloge of Surgeons of the 17th oint Congress of Asia & Pacific Federations & the 53rd Annual Congress Of the Japan sectlon
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2007-06-09
• [Presentation] Circadian rhythm in the larynx and trachea under the control of molecular clock (2006)
  • Author(s): Hisa Y, Bando H, Nishio T, Koike S, Bamba H
  • Organizer: Collegium Oto-Rhino-Laryngologicum Amicitiae Sacrum
  • Place of Presentation: Moscow, Russia
  • Year and Date: 2006-08-30
• [Presentation] Locaization of fibroblast growth factor-1 in cholinergic neurons innervating the rat larynx(poster) (2006)
  • Author(s): Okano H, Toyoda K, Bamba H, Hisa Y, Oomura Y, Imamura T, Furukawa S, Kimura H, Tooyama I
  • Organizer: The 7th Joint Meeting of the Histochemical Society & the Japan Society Of Histochemistry and Cytochemistry
  • Place of Presentation: Hawail, USA
  • Year and Date: 2006-08-24
• [Presentation] Localization of fibroblast growth factor-1(FGF1)in cholinergic neurons innervating the rat larynx(poster) (2006)
  • Author(s): Okano H, ToyOda K, Bamba H, Hisa Y, Oomura Y, Imamura T, Furukawa S, Kimura H, Tooyama I
  • Organizer: The 7th Joint meeting of the Histochemical Society and the Japan Soclety of Histochemlstry and Cytochemistry
  • Place of Presentation: Hawali, USA
  • Year and Date: 2006-08-24
• [Presentation] Circadian rhythm in the laryngeal function under the control of molecular clock(poster) (2006)
  • Author(s): Bando H, Nishio T, Okamura H, Hirota R, Bamba H, Hisa Y
  • Organizer: The 5th lnternational Conference on Voice Physiology and Blomechanics
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2006-07-12