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The role of innate immunity in choroidal neovascularization

Research Project

Project/Area Number 18390469
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKyushu University

Principal Investigator

SONODA Koh-hei  Kyushu University, Graduate School of Medical Science, Assistant Professor (10294943)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Hiroki  Saga University, Faculty of Medicine, Professor (40260715)
EGASHIRA Kensuke  KYUSHU UNIVERSITY, Graduate School of Medical Science, Associate Professor (60260379)
YONEMITSU Yoshikazu  Chiba University, Graduate School of Medical Science, Professor (40315065)
HATA Yasuaki  KYUSHU UNIVERSITY, Graduate School of Medical Science, Associate Professor (90346776)
ISHIBASHI Tatsuro  KYUSHU UNIVERSITY, Graduate School of Medical Science, Professor (30150428)
池田 康博  九州大学, 医学研究院, 助教 (20380389)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥11,220,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2007: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2006: ¥5,500,000 (Direct Cost: ¥5,500,000)
Keywordscorneal transplantation / NKT cells / cytokine / tolerance / DNA chip / gene transfer / immune therapy
Research Abstract

1: The establishment of subretinal scaring model in mice.
Methods
Under the microscope, we inoculated peritoneal exudate cells (PECs). PECs were obtained from peritoneal washes of either B6 mice, three days after they were inoculated intraperitoneally (i.p.) with 2.5ml of an aged solution of 3% thioglycollate. Seven days later, the subretinal scaring was observed.
Evaluation
We stained scaring parts by Glia1 Fibriary Acidic Protein (GFAP), the marker of glia1 cells. Then we measure the area and valuate scaring.
2: Identification of local accumulating cells and the screening of cytokine and chemokines produced by these cells.
Inflammatory macrophages and transformed retinal pigment epithelium (RPE). We screened specific cytokines and chemokines by RNAse protection assay and multiplex protein beads assay. We found both macrophages and RPE predominantly produced IL-6 and MCP-1.
3: The suppression of subretinal scaring by blocking of IL-6.
Systemic treatment of IL-6 blocking antibody reduced the size of scaring. Also we inoculated IL-10-trsformed RPEs into the vitreous cavity for therapeutic purpose, and confirmed the reduction of scaring.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (4 results)

All 2008 2007

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] 眼炎症と自然免疫2008

    • Author(s)
      園田 康平
    • Journal Title

      日本眼科学会雑誌 112

      Pages: 279-298

    • NAID

      10019478273

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The association of ocular inflammation and innate immune response2008

    • Author(s)
      Sonoda, KH
    • Journal Title

      Nippon Ganka Gakkai Zasshi 112(3)

      Pages: 279-97

    • NAID

      10021292215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 眼炎症と自然免疫2007

    • Author(s)
      園田 康平
    • Organizer
      第111回日本眼科学会総会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2007-04-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The association of ocular inflammation and innate immune response2007

    • Author(s)
      Sonoda, KH
    • Organizer
      The 111th Annual meeting of the Japanese Ophthalmology Society Osaka
    • Place of Presentation
      Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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