The role of innate immunity in choroidal neovascularization

• Project/Area Number: 18390469
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kyushu University
• Principal Investigator: SONODA Koh-hei Kyushu University, Graduate School of Medical Science, Assistant Professor (10294943)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Hiroki Saga University, Faculty of Medicine, Professor (40260715) ; EGASHIRA Kensuke KYUSHU UNIVERSITY, Graduate School of Medical Science, Associate Professor (60260379) ; YONEMITSU Yoshikazu Chiba University, Graduate School of Medical Science, Professor (40315065) ; HATA Yasuaki KYUSHU UNIVERSITY, Graduate School of Medical Science, Associate Professor (90346776) ; ISHIBASHI Tatsuro KYUSHU UNIVERSITY, Graduate School of Medical Science, Professor (30150428) ; 池田 康博 九州大学, 医学研究院, 助教 (20380389)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥11,220,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥1,320,000); Fiscal Year 2007: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2006: ¥5,500,000 (Direct Cost: ¥5,500,000)
• Keywords: corneal transplantation / NKT cells / cytokine / tolerance / DNA chip / gene transfer / immune therapy

Research Abstract

1: The establishment of subretinal scaring model in mice.
Methods
Under the microscope, we inoculated peritoneal exudate cells (PECs). PECs were obtained from peritoneal washes of either B6 mice, three days after they were inoculated intraperitoneally (i.p.) with 2.5ml of an aged solution of 3% thioglycollate. Seven days later, the subretinal scaring was observed.
Evaluation
We stained scaring parts by Glia1 Fibriary Acidic Protein (GFAP), the marker of glia1 cells. Then we measure the area and valuate scaring.
2: Identification of local accumulating cells and the screening of cytokine and chemokines produced by these cells.
Inflammatory macrophages and transformed retinal pigment epithelium (RPE). We screened specific cytokines and chemokines by RNAse protection assay and multiplex protein beads assay. We found both macrophages and RPE predominantly produced IL-6 and MCP-1.
3: The suppression of subretinal scaring by blocking of IL-6.
Systemic treatment of IL-6 blocking antibody reduced the size of scaring. Also we inoculated IL-10-trsformed RPEs into the vitreous cavity for therapeutic purpose, and confirmed the reduction of scaring.

Research Products

• [Journal Article] 眼炎症と自然免疫 (2008)
  • Author(s): 園田 康平
  • Journal Title: 日本眼科学会雑誌 112 Pages: 279-298
  • NAID: 10019478273
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The association of ocular inflammation and innate immune response (2008)
  • Author(s): Sonoda, KH
  • Journal Title: Nippon Ganka Gakkai Zasshi 112(3) Pages: 279-97
  • NAID: 10021292215
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 眼炎症と自然免疫 (2007)
  • Author(s): 園田 康平
  • Organizer: 第111回日本眼科学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2007-04-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The association of ocular inflammation and innate immune response (2007)
  • Author(s): Sonoda, KH
  • Organizer: The 111th Annual meeting of the Japanese Ophthalmology Society Osaka
  • Place of Presentation: Japan
  • Description: 「研究成果報告書概要(欧文)」より