The Clarification of the expression mechanism of functional molecules on human lymphatic endothelium in oral cavity

• Project/Area Number: 18390483
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Hokkaido University
• Principal Investigator: YOSHIDA Shigemitsu Hokkaido University, 名誉教授 (80174928)
• Co-Investigator(Kenkyū-buntansha): ISHISAKI Akira 岩手医科大学, 歯学部, 教授 (20356439) ; DOMON Takanori 北海道大学, 大学院・歯学研究科, 教授 (50217618) ; ASHIKAGA Yuichi 北海道大学, 大学院・歯学研究科, 助教 (70372258) ; INOUE Kiichiro 北海道大学, 大学院・歯学研究科, 助教 (30168439) ; KUROSHIMA Shinichiro 北海道大学, 大学院・歯学研究科, 助教 (40443915)
• Co-Investigator(Renkei-kenkyūsha): SAWA Yoshihiko 福岡歯科大学, 歯学部, 教授 (70271666)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥17,560,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥1,860,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2006: ¥9,500,000 (Direct Cost: ¥9,500,000)
• Keywords: リンパ管内皮細胞 / 白血球接着因子 / ケモカイン / 口腔組織 / 毛細リンパ管 / Toll様受容体 / 転写因子 / リンホトキシン / 口腔健常組織 / 口腔炎症組織

Research Abstract

We investigated some proteins which play an important role in the immune system to clarify immune mechanisms via intraoral and extraoral lymphatic vessels. We showed that intraoral lymphatic vessels may differently play site-specific roles in immune systems, and extraoral lymphatic vessels have intracellular networks through these proteins. We discovered that systemic lymphatic vessels contribute to not only substance transport, but also immune mechanisms.

Research Products

• [Journal Article] Vascular cell-like potential of undifferentiated ligament fibroblasts to construct vascular cell-specific marker-positive blood vessel structures in a PI3K-activation-dependent manner (2010)
  • Author(s): Okubo N, Ishisaki A, Iizuka T, Tamura M, Kitagawa Y.
  • Journal Title: J. Vasc. Res. 47 Pages: 369-383
  • NAID: 120002400574
  • Peer Reviewed
• [Journal Article] Vascular cell-like potential of undifferentiated ligament fibroblasts to construct vascular cell-specific marker-positive blood vessel structures in a PI3K-activation-dependent manner (2010)
  • Author(s): Okubo, N., Ishisaki, A., Iizuka, T., Tamura, M., Kitagawa, Y.
  • Journal Title: Journal of Vascular Research 47 Pages: 369-383
  • NAID: 120002400574
  • Peer Reviewed
• [Journal Article] Synergistic effect of dexamethasone and prolactin on VEGF expression bovine mammary epithelial cells via p44/p42 MAP kinase (2009)
  • Author(s): Nakajima K, Nakamura M, Ishisaki A, Kozakai T.
  • Journal Title: Asian-Australasian Journal of Animal Sciences 22 Pages: 788-795
  • Peer Reviewed
• [Journal Article] 骨における血管新生と線溶系 (総説) (2009)
  • Author(s): 帖佐直幸, 石崎明
  • Journal Title: 日本血栓止血学会誌 20 Pages: 12-17
  • Peer Reviewed
• [Journal Article] 歯根膜由来幹細胞様細胞による3次元培養下における血管内皮細胞マーカー陽性血管様構造の形成 (2009)
  • Author(s): 大久保直登, 帖佐直幸, 客本斉子, 高橋典子, 加茂政晴, 石崎明
  • Journal Title: 口腔組織培養学会誌 19 Pages: 29-30
  • Peer Reviewed
• [Journal Article] Synergistic effect of dexamethasone and prolactin on VEGF expression bovine mammary epithelial cells via p44/p42 MAP kinase (2009)
  • Author(s): Nakajima, K., Nakamura, M., Ishisaki, A., Kozakai, T.
  • Journal Title: Asian-Australasian Journal of Animal Sciences 22 Pages: 788-795
  • Peer Reviewed
• [Journal Article] 骨における血管新生と線溶系(総説) (2009)
  • Author(s): 帖佐直幸, 石崎明
  • Journal Title: 日本血栓止血学会誌 20 Pages: 12-17
  • Peer Reviewed
• [Journal Article] 歯根膜由来幹細胞様細胞による3次元培養下における血管内皮細胞マーカー陽性血管様構造の形成 (2009)
  • Author(s): 大久保直登、帖佐直幸、客本斉子、高橋典子、加茂政晴、石崎明
  • Journal Title: 口腔組織培養学会誌 19 Pages: 29-30
  • Peer Reviewed
• [Journal Article] Leukocyte adhesion molecule and chemokine production through lipoteichoic acid recognition by toll-like receptor 2 in cultured human lymphatic endothelium (2008)
  • Author(s): Sawa Y, Tsuruga E, Iwasawa K, Ishikawa H, Yoshida S.
  • Journal Title: Cell Tissue Res. 333(2) Pages: 237-252
  • Peer Reviewed
• [Journal Article] LPS-induced IL-6, IL-8, VCAM-1, and ICAM-1 expression in human lymphatic endothelium (2008)
  • Author(s): Sawa Y, Ueki T, Hata M, Iwasawa K, Tsuruga E, Kojima H, Ishikawa H, Yoshida S.
  • Journal Title: J Histochem Cytochem 56(2) Pages: 97-109
  • Peer Reviewed
• [Journal Article] Distribution and organization of lymphatic vessels in the mouse gingiva : An immunohistochemical study with LYVE-1 (2008)
  • Author(s): Ushijima N, Inoue K, Domon T, Yoshida S.
  • Journal Title: Arch. Oral Boil. 53(7) Pages: 652-658
  • Peer Reviewed
• [Journal Article] Expression of CCL21, TLR2, and TLR4 on human lingual lymphatic endothelium (2008)
  • Author(s): Taishi Y, Kuroshima S, Domon T, Yoshida S, Kitagawa Y.
  • Journal Title: Hokkaido J. Dent. Sci. 29(2) Pages: 197-208
  • NAID: 10026161701
  • Peer Reviewed
• [Journal Article] Expression of CCL21, TLR2, and TLR4 on human lingual lymphatic endothelium (2008)
  • Author(s): Taishi Y, Kuroshima S, Domon T, Yoshida S, Kitagawa Y
  • Journal Title: Hokkaido J. Dent. Sci. 29(2) Pages: 197-208
  • NAID: 10026161701
  • Peer Reviewed
• [Journal Article] Expression of CCL21, TLR2, and TLR4 on human lingual lymphatic endothelium (2008)
  • Author(s): Taishi Y, Kuroshima S, Domon T, Yoshida S, Kitagawa Y.
  • Journal Title: Hokkaido Journal of Dental Science 29 Pages: 197-208
  • NAID: 10026161701
  • Peer Reviewed
• [Journal Article] Distribution and organization of lymphatic vessels in the mouse gingiva : An immunohistochemical study with LYVE-1. (2008)
  • Author(s): Ushijima N, Inoue K, Domon T, Yoshida S.
  • Journal Title: Archives of Oral Biology 53 Pages: 652-658
  • Peer Reviewed
• [Journal Article] Leukocyte adhesion molecule and chemokine production through lipoteichoic acid recognition by toll-like receptor 2 in cultured human lymphatic endothelium (2008)
  • Author(s): Sawa Y, Tsuruga E, Iwasawa K, Ishikawa H, Yoshida S
  • Journal Title: Cell and Tissue Research 333 Pages: 237-252
  • Peer Reviewed
• [Journal Article] LPS-induced IL-6, IL-8, VCAM-1, and ICAM-1 expression in human lymphatic endothelium. (2008)
  • Author(s): Sawa Y, Ueki T, Hata M, Iwasawa K, Tsuruga E, Kojima H, Ishikawa H, Yoshida S
  • Journal Title: The journal of histochemistry and cytochemistry 56(2) Pages: 97-109
  • Peer Reviewed
• [Journal Article] Effects of TNF-alpha on leukocyte adhesion molecule expressions in cultured human lymphatic endothelium (2007)
  • Author(s): Sawa Y, Sugimoto Y, Ueki T, Ishikawa H, Sato A, Nagato T, Yoshida S.
  • Journal Title: J Histochem Cytochem. 55(7) Pages: 721-33
  • Peer Reviewed
• [Journal Article] Effects of TNF-{alpha} on Leukocyte Adhesion Molecule Expressions in Cultured Human Lymphatic Endothelium. (2007)
  • Author(s): Sawa Y, Sugimoto Y, Ueki T, Ishikawa H, Sato A, Nagato T, Yoshida S
  • Journal Title: The journal of histochemistry and cytochemistry (Epub ahead of print)
• [Presentation] ヒト扁平上皮癌細胞におけるガレクチン-1とEGFRの相互作用によるアノイキス抑制作用 (2009)
  • Author(s): 加茂政晴, 陳明珠, 客本斉子, 帖佐直幸, 高橋典子, 大久保直登, 石崎明
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] ヒト扁平上皮癌細胞におけるガレクチン-1とEGFRの相互作用によるアノイキス抑制作用 (2009)
  • Author(s): 加茂政晴、陳明珠、客本斉子、帖佐直幸、高橋典子、大久保直登、石崎明
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市,パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] 間葉系幹細胞におけるPDGFレセプターを介したVCAM1の発現解析 (2009)
  • Author(s): 西平宗功, 大久保直登, 高橋典子, 客本斉子, 加茂政晴, 長谷川智一, 杉山芳樹, 石崎明, 帖佐直幸
  • Organizer: 日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] 間葉系幹細胞におけるPDGFレセプターを介したVCAM1の発現解析 (2009)
  • Author(s): 西平宗功、大久保直登、高橋典子、客本斉子、加茂政晴、長谷川智一、杉山芳樹、石崎明、帖佐直幸
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸市,神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] 歯根膜由来幹細胞による血管内皮細胞マーカー陽性血管構造の形成 (2009)
  • Author(s): 大久保直登, 田村正人, 飯塚正, 加茂政晴, 客本斉子, 帖佐直幸, 高橋典子, 北川善政, 石崎明
  • Organizer: 歯科基礎医学会学会
  • Place of Presentation: 朱鷺メッセ : 新潟コンベンションセンター
  • Year and Date: 2009-09-10
• [Presentation] 歯根膜由来幹細胞による血管内皮細胞マーカー陽性血管構造の形成 (2009)
  • Author(s): 大久保直登、田村正人、飯塚正、加茂政晴、客本斉子、帖佐直幸、高橋典子、北川善政、石崎明
  • Organizer: 第51回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 新潟市,朱鷺メッセ:新潟コンベンションセンター
  • Year and Date: 2009-09-10
• [Presentation] ヒト舌リンパ管における Toll-like receptor 2および4の発現 (2008)
  • Author(s): 太子芳仁, 黒嶋伸一郎, 土門卓文, 吉田重光, 北川善政
  • Organizer: 歯科基礎医学会
  • Place of Presentation: TOC有明
  • Year and Date: 2008-09-25
• [Presentation] ヒト舌リンパ管におけるToll-like receptor 2および4の発現 (2008)
  • Author(s): 太子芳仁、黒嶋伸一郎、土門卓文、吉田重光、北川善政
  • Organizer: 歯科基礎医学会
  • Place of Presentation: TOC有明
  • Year and Date: 2008-09-25
• [Presentation] ヒト舌リンパ管におけるToll-like receptor 2および4の発現 (2008)
  • Author(s): 太子芳仁、黒嶋伸一郎、土門卓文、吉田重光、北川善政
  • Organizer: 歯科基礎医学会
  • Place of Presentation: TOC有明コンベンションホール
  • Year and Date: 2008-09-25
• [Presentation] ヒト歯肉リンパ管における Toll-like receptor 2および4の発現 (2008)
  • Author(s): 黒嶋伸一郎, 太子芳仁, 吉田重光, 井上農夫男
  • Organizer: 歯科基礎医学会
  • Place of Presentation: TOC有明
  • Year and Date: 2008-09-23
• [Presentation] ヒト歯肉リンパ管におけるToll-like receptor 2および4の発現 (2008)
  • Author(s): 黒嶋伸一郎、太子芳仁、吉田重光、井上農夫男
  • Organizer: 歯科基礎医学会
  • Place of Presentation: TOC有明
  • Year and Date: 2008-09-23
• [Presentation] ヒト歯肉リンパ管におけるToll-like receptor 2および4の発現 (2008)
  • Author(s): 黒嶋伸一郎、太子芳仁、吉田重光、井上農夫男
  • Organizer: 歯科基礎医学会
  • Place of Presentation: TOC有明コンベンションホール
  • Year and Date: 2008-09-23
• [Presentation] マウス歯肉における毛細リンパ管の免疫組織学的検索 (2007)
  • Author(s): 牛島夏未, 井上貴一朗, 土門卓文, 高橋智美, 本間善幸, 飯塚正, 吉田重光
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2007-08-31
• [Presentation] マウス歯肉におけるリンパ管分布 (2006)
  • Author(s): 牛島夏未, 土門卓文, 井上貴一朗, 吉田重光
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 鶴見大学記念館
  • Year and Date: 2006-09-22