Protective immunity against group Astreptococcus infection following immunization with fibronectin-binding proteins
| Project/Area Number |
18390489
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
KAWABATA Shigetada Osaka University, Department of Oral and Molecular Microbiology, Professor (50273694)
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| Co-Investigator(Kenkyū-buntansha) |
TERAO Yutaka Osaka University, Department of Oral and Molecular Microbiology, Associate Professor (50397717)
NEKATA Masanobu Osaka University, Depoartment of Oral and Molecular Miaobiology, Assistant Professor (90444497)
SUMITOMO Tomoko Osaka University, Department of Oral and Molecular Microbiology, Assistant Professor (50423421)
OKAMOTO Shigefumi National Institute of Biomedical Innovation, Division of Biomedical Research, Researcher (50311759)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,940,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2006: ¥12,000,000 (Direct Cost: ¥12,000,000)
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| Keywords | Streptococcus pyogenes / fibronectin-binding protein / FbaA / FbaB / group A streptococci / vaccine / cell-associated protein / potective antigen / フィブロネクチン結合タンパク / 菌体表層タンパク |
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| Research Abstract |
Background. Surface-associated fibronectin (Fn) -binding proteins of group A Streptococcus pyogenes play important roles in the bacterial invasion of epithelial cells. The aim of this study was to examine the functional domain and protective antigenicity of the Fn-binding proteins FbaA and FbaB.
Methods. To investigate the functional domain of FbaA or FbaB and their localization on S. pyogenes, a series of recombinant GST-truncated FbaA or FbaB proteins were employed for immunofluorescent microscopy, ligand blot, and Biacore analyses. Mice were immunized with the truncated proteins to determine the immunogenic domains that contribute to protection against S. pyogenes infection.
Results. Ligand blot and Biacore analyses revealed that the FbaA fragments harboring a proline-rich repeat domain (RD), but not the N- and C-terminal regions, possessed Fn-binding activity. Immunofluorescent microscopy findings showed that the N-terminus and RD were exposed to external regions, suggesting that the RD serves as an Fn-binding element on live organisms. Specific antibodies were efficiently induced in N-terminus- and RD-immunized mice and demonstrated bactericidal activity against S. pyogenes in vitro. FbaA-immunized mice survived significantly longer as compared to the GST-immunized group following infection with M1 and M49 strains expressing FbaA. In addition, FbaB-immunized mice also survived longer than control mice after challenge with M3 strain expressing FbaB.
Conclusion. The Fn-binding proteins, FbaA and FbaB, are potential vaccine candidates for S. pyogenes infection.
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Report
(3 results)
Research Products
(50 results)
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[Journal Article] Group A streptococcal cysteine protease degrades C3(C3b)and contributes to evasion of innate immunity.2008
Author(s)
Terao, Y., Mori, Y., Yamaguchi, M., Shimizu, Y., Ooe, K., Hamada, S. Kawabata, S.
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Journal Title
Journal of Biological Chemistry 283
Pages: 6253-6260
Description
「研究成果報告書概要(和文)」より
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Peer Reviewed
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[Journal Article] Molecular and biological characterization of histidine triad protein in group A streptococci.2008
Author(s)
Kunitomo, E., Terao, Y., Okamoto, S., Rikimaru, T., Hamada, S., Kawabata, S.
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Journal Title
Microbes and Infections 10
Pages: 414-423
Description
「研究成果報告書概要(和文)」より
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Peer Reviewed
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[Journal Article] The Streptococcus pyogenes serotype M49 Nra-Ralp3 transcriptional regulatory network and its control of virulence factor expression from the novel eno ralp3 epf sagA pathogenicity region2008
Author(s)
Kreikemeyer, B., Nakata, M., Miller, T., Hildisch, H., Kourakos, V., Standar, K., Kawabata, S., Glocker, M. 0., Podbielski, A
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Journal Title
Infection and Immunity 75
Pages: 5698-5710
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] 「研究成果報告書概要(欧文)」より2008
Author(s)
Terao, Y., Mori, Y., Yamaguchi, M., Shimizu, Y., Ooe, K., Hamada, S., Kawabata, S
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Journal Title
The Journal of Biological Chemistry 283
Pages: 6253-6260
Related Report
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[Journal Article] Molecular and biological characterization of histidine triad protein in group A streptococci2008
Author(s)
Kunitomo, E., Terao, Y., Okamoto, S., Rikimaru, T., Hamada, S., Kawabata, S
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Journal Title
Microbes and Infection 10
Pages: 414-423
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Group A streptococcal cysteine protease degrades C3 (C3b) and contributes to evasion of innate immunity.2008
Author(s)
Terao, Y., Mori, Y., Yamaguchi, M., Shimizu, Y., Ooe, K., Hamada, S. and Kawabata S.
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Journal Title
Journal of Biological Chemistry (印刷中)
Related Report
Peer Reviewed
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[Journal Article] Molecular and biological characterization of histidine triad protein in group A streptococci.2008
Author(s)
Kunitomo, E., Terao, Y., Okamoto, S., Rikimaru, T., Hamada, S., Kawabata, S.
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Journal Title
Microbes and Infections (印刷中)
Related Report
Peer Reviewed
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[Journal Article] Calcineurin potentiates the activation of procaspase-3 by accelerating its proteolytic maturation.2007
Author(s)
Saeki, M., Irie, Y. Ni, L. Itsuki, Y. Terao, Y., Kawabata, S., Kamisaki, Y.
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Journal Title
Journal of Biological Chemistry 282
Pages: 11786-11794
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] The Streptococcus pyogenes serotype M49 Nra-Ralp3 transcriptional regulatory network and its control of virulence factor expression from the novel eno ralp3 epf sagA pathogenicity region.2007
Author(s)
Kreikemeyer, B., Nakata, M., Koller, T., Hildisch, H., Kourakos, V., Standar, K., Kawabata, S., Glocker, M. O. Podbielski, A.
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Journal Title
Infection and Immunity 75
Pages: 5698-5710
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
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[Journal Article] Calcineurin potentiates the activation of procaspase-3 by accelerating its proteolytic maturation2007
Author(s)
Saeki, M., Irie, Y., Ni, L., Itsuki, Y., Terao, Y., Kawabata, S., Kamisaki, Y
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Journal Title
The Journal of Biological Chemistry 282
Pages: 11786-11794
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] The Streptococcus pyogenes serotype M49 Nra-Ralp3 transcriptional regulatory network and its control of virulence factor expression from the novel eno ralp3 epf sagA pathogenicity region.2007
Author(s)
Kreikemeyer, B., Nakata, M., Koller, T., Hildisch, H., Kourakos, V., Standar, K., Kawabata, S., Glocker, M.O. and Podbielski, A.
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Journal Title
Infection and Immunity 75
Pages: 5698-5710
Related Report
Peer Reviewed
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[Journal Article] Role of Streptococcus sanguinis sortase A in bacterial colonization.2006
Author(s)
Yamaguchi, M., Terao, Y., Ogawa, T., Takahashi, T., Hamada, S., Kawabata, S.
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Journal Title
Microbes and Infection 8
Pages: 2791-2796
Description
「研究成果報告書概要(和文)」より
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Peer Reviewed
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[Journal Article] Role of Streptococcus sanguinis sortase A in bacterial colonization2006
Author(s)
Yamaguchi, M., Terao, Y., Ogawa, T., Takahashi, T., Hamada, S., Kawabata, S
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Journal Title
Microbes and Infection 8
Pages: 2791-2796
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Plasmin(ogen)-and fibronectin-binding protein of Streptococcus pneumoniae provides bacterial plasmin activity2007
Author(s)
Yamaguchi, M., Terao, Y., Mori, Y., Hamada, S., Kawabata, S.
Organizer
107th General Meeting American Society for Microbiology
Place of Presentation
Toronto, Canada
Description
「研究成果報告書概要(和文)」より
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[Presentation] Role of Streptococcus pyogenes FCT-region sortase and other components in pilus assembly and virulence.2007
Author(s)
Nakata, M., Ribardo, D. A., Mclver, K. S., Kawabata, S., Podbielski, A., Kreikemeyer, B.
Organizer
107th General Meeting American Society for Microbiology
Place of Presentation
Toronto, Canada
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Plasmin(ogen)- and fibronectin-binding protein of Streptococcus pneumoniae provides bacterial plasmin activity2007
Author(s)
Yamaguchi, M., Terao, Y., Mori, V., Hamada, S., Kawabata, S
Organizer
107th General Meeting American Society for Microbiology
Place of Presentation
Toronto, Canada
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Role of Streptococcus pyogenes FCT-region sortase and other components in pilus assembly and virulence2007
Author(s)
Nakata, M., Ribardo, D.A., Mclver, K.S., Kawabata, S., Podbielski, A., Kreikemeyer, B
Organizer
107th General Meeting American Society for Microbiology
Place of Presentation
Toronto, Canada
Description
「研究成果報告書概要(欧文)」より
Related Report
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